scholarly journals Reference transcriptome data in silkworm Bombyx mori

2019 ◽  
Author(s):  
Kakeru Yokoi ◽  
Takuya Tsubota ◽  
Jianqiang Sun ◽  
Akiya Jouraku ◽  
Hideki Sezutsu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Reference Genome ◽  
Enrichment Analysis ◽  
Silk Gland ◽  
Transcriptome Data ◽  
Data Set ◽  
Reference Transcriptome ◽  
Genome Data ◽  
Silk Glands ◽  
Sequence Region

AbstractThe silkworm Bombyx mori has long been used in the silk industry and utilized in studies of physiology, genetics, molecular biology, and pathology. We recently reported high quality reference genome data for B. mori. In the present study, we constructed a reference transcriptome data set using the reference genome data and RNA-seq data of 10 tissues from P50T strain larvae. Reference transcriptome data contained 51,926 transcripts, with 39,619 having one or more coding sequence region. The abundance of each transcript in the 10 tissues as well as 5 tissues from other strain larvae was estimated, and hierarchical clustering was performed. The results obtained showed that data on abundance were highly reproducible and there here is a little difference of transcriptome abundance between the two strain larvae. New isoforms of silk-related genes were searched for in the reference transcriptomes, and the longest isoform of sericin-1 possessing a long exon was identified. We also extracted transcripts that were strongly expressed in one or more parts of the silk glands. An enrichment analysis performed using the functional annotation data of the transcripts provided novel insights into the functions of the silk gland parts. Therefore, the reference transcriptome data set obtained has extended B. mori genomic and transcriptomic insights and may contribute to advances in entomologic and vertebrate research, including that on humans.

scholarly journals A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori

2022 ◽  
Vol 12 ◽  
Author(s):  
Zhiqian Li ◽  
Lang You ◽  
Qichao Zhang ◽  
Ye Yu ◽  
Anjiang Tan
Keyword(s):  
Bombyx Mori ◽  
Recombinant Proteins ◽  
Expression System ◽  
Silk Gland ◽  
Fusion Expression ◽  
Transcription Activator ◽  
Genomic Locus ◽  
Silk Proteins ◽  
Silk Glands ◽  
New Strategy

The domesticated silkworm, Bombyx mori, is an economically important insect that synthesizes large amounts of silk proteins in its silk gland to make cocoons. In recent years, germline transformation strategies advanced the bioengineering of the silk gland as an ideal bioreactor for mass production of recombinant proteins. However, the yield of exogenous proteins varied largely due to the random insertion and gene drift caused by canonical transposon-based transformation, calling for site-specific and stable expression systems. In the current study, we established a targeted in-fusion expression system by using the transcription activator-like effector nuclease (TALEN)-mediated targeted insertion to target genomic locus of sericin, one of the major silk proteins. We successfully generated chimeric Sericin1-EGFP (Ser-2A-EGFP) transformant, producing up to 3.1% (w/w) of EGFP protein in the cocoon shell. With this strategy, we further expressed the medically important human epidermal growth factor (hEGF) and the protein yield in both middle silk glands, and cocoon shells reached to more than 15-fold higher than the canonical piggyBac-based transgenesis. This natural Sericin1 expression system provides a new strategy for producing recombinant proteins by using the silkworm silk gland as the bioreactor.

scholarly journals Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259870
Author(s):  
Yan Ma ◽  
Qiwei Sun ◽  
Lihua Huang ◽  
Qin Luo ◽  
Wenhui Zeng ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Bombyx Mori ◽  
Large Scale ◽  
Expression Patterns ◽  
Silk Gland ◽  
Cellular Functions ◽  
Chromosomal Distribution ◽  
Genome Data ◽  
Silkworm Genome ◽  
Full Length Transcript

Transcription factors (TFs) are key proteins that modulate gene transcription and thereby lead to changes in the gene expression profile and the subsequent alteration of cellular functions. In the silk gland (SG) of silkworm Bombyx mori, an important silk-producing insect, TFs are of vital importance in the regulation of silk protein synthesis in this organ. However, which TFs exist and express in the SG remains largely unknown. Here, we report the large-scale identification of TFs in the SG based on available full-length transcript sequences and the most recent version of silkworm genome data. In total, 348 candidate TFs were identified by strict filtration and were classified into 56 TF families. Chromosomal distribution, motif composition, and phylogenetic relationship analyses revealed the typical characteristics of these TFs. In addition, the expression patterns of 348 TFs in various tissues of B. mori, especially the SG of fourth-molt (4LM) and day-3 and day-4 fifth-instar (5L3D and 5L4D) larvae, were investigated based on public RNA-seq data and gene microarray data, followed by spatiotemporal verification of TF expression levels by quantitative real-time PCR (qRT-PCR). This report describes the first comprehensive analysis of TFs in the B. mori SG. The results can serve as a baseline for further studies of the roles of TFs in the B. mori SG.

TREHALASE ACTIVITY IN THE SILK GLANDS OF THE SILKWORM, BOMBYX MORI (LEPIDOPTERA: BOMBYCIDAE)

1975 ◽  
Vol 107 (12) ◽  
pp. 1311-1314 ◽  
Author(s):  
Shuya Shimada
Keyword(s):  
Bombyx Mori ◽  
Divalent Cations ◽  
Silk Gland ◽  
Trehalase Activity ◽  
Gland Cells ◽  
Silk Glands ◽  
Tunica Propria ◽  
Silkworm Bombyx Mori ◽  
Cu And Zn ◽  
Tunica Intima

Abstract(1) Crude extracts prepared from the silk glands of the silkworm, Bombyx mori L. contain trehalase activity. (2) Trehalase in the silk glands has a pH of 5.5 and a Km of 0.71 mM. The activity of the enzyme is inhibited by divalent cations such as Mn, Cu, and Zn. (3) By histochemical methods, it is shown that trehalase is localized in the periphery of the silk gland cells, especially in the tunica propria and tunica intima. (4) Trehalase activity is low in fifth instar and increases greatly in spinning stages, after which the activity decreases.

Expression of autophagy-related genes in the anterior silk gland of the silkworm (Bombyx mori) during metamorphosis

2011 ◽  
Vol 89 (11) ◽  
pp. 1019-1026 ◽  
Author(s):  
Qingrong Li ◽  
Xiaojuan Deng ◽  
Zhijun Huang ◽  
Sichun Zheng ◽  
Gianluca Tettamanti ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Morphological Characteristics ◽  
Time Frame ◽  
Silk Gland ◽  
Marker Genes ◽  
Reverse Transcription Pcr ◽  
Evolutionarily Conserved ◽  
Silk Glands ◽  
Autophagic Process ◽  
Silkworm Bombyx Mori

Programmed cell death (PCD) is a genetically regulated process of cell elimination and is evolutionarily conserved in eukaryotes. Degeneration of larval tissues during metamorphosis in insects is a result of PCD triggered by ecdysteroids and autophagic process has been shown to be involved in the degeneration of silk gland of the silkworm ( Bombyx mori L., 1758). However, experimental evidence for the expression of autophagy marker genes remains insufficient. In this study, expression of the autophagy-related genes BmAtg5, BmAtg6, BmAtg8, and BmAtg12 was determined in the anterior silk glands of larvae and pupae during larval to pupal transformation by using reverse-transcription PCR, quantitative real-time PCR, and Western blot analyses. The results indicated that BmAtg5, BmAtg6, BmAtg8, and BmAtg12 had the highest expression levels on the 3rd day of the wandering stage or at the prepupal stage, and the time frame of the expression of these genes was coincident with the morphological characteristics of autophagy in the silk gland during the larval to pupal transformation. This study demonstrated that the autophagy-related genes are involved in the degeneration of the anterior silk gland of B. mori.

scholarly journals Reference Transcriptome Data in Silkworm Bombyx mori

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 519
Author(s):  
Kakeru Yokoi ◽  
Takuya Tsubota ◽  
Akiya Jouraku ◽  
Hideki Sezutsu ◽  
Hidemasa Bono
Keyword(s):  
Expression Profiles ◽  
Complex Structure ◽  
Complete Sequence ◽  
Silk Gland ◽  
Transcriptome Data ◽  
Silkworm Larvae ◽  
Tandem Gene Duplication ◽  
Reference Transcriptome ◽  
Basic Studies ◽  
Entomological Research

Herein, we performed RNA-seq analysis of ten major tissues/subparts of silkworm larvae. The sequences were mapped onto the reference genome assembly and the reference transcriptome data were successfully constructed. The reference data provided a nearly complete sequence for sericin-1, a major silk gene with a complex structure. We also markedly improved the gene model for other genes. The transcriptomic expression was investigated in each tissue and a number of transcripts were identified that were exclusively expressed in tissues such as the testis. Transcripts strongly expressed in the midgut formed tight genomic clusters, suggesting that they originated from tandem gene duplication. Transcriptional factor genes expressed in specific tissues or the silk gland subparts were also identified. We successfully constructed reference transcriptome data in the silkworm and found that a number of transcripts showed unique expression profiles. These results will facilitate basic studies on the silkworm and accelerate its applications, which will contribute to further advances in lepidopteran and entomological research as well as the practical use of these insects.

Integrated Quantitative Analysis of the Phosphoproteome and Transcriptome in Tamoxifen-resistant Breast Cancer*

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Breast Cancer ◽  
High Rate ◽  
System Level ◽  
Clinical Samples ◽  
Transcriptome Data ◽  
Data Set ◽  
Reporter Assays ◽  
Treated Breast ◽  
Mcf 7 ◽  
Resistant Cells

Quantitative phosphoproteome and transcriptome analysisof ligand-stimulated MCF-7 human breast cancer cells wasperformed to understand the mechanisms of tamoxifen resistanceat a system level. Phosphoproteome data revealed thatWT cells were more enriched with phospho-proteins thantamoxifen-resistant cells after stimulation with ligands.Surprisingly, decreased phosphorylation after ligand perturbationwas more common than increased phosphorylation.In particular, 17?-estradiol induced down-regulation inWT cells at a very high rate. 17?-Estradiol and the ErbBligand heregulin induced almost equal numbers of up-regulatedphospho-proteins in WT cells. Pathway and motifactivity analyses using transcriptome data additionallysuggested that deregulated activation of GSK3? (glycogensynthasekinase 3?) and MAPK1/3 signaling might be associatedwith altered activation of cAMP-responsive elementbindingprotein and AP-1 transcription factors intamoxifen-resistant cells, and this hypothesis was validatedby reporter assays. An examination of clinical samples revealedthat inhibitory phosphorylation of GSK3? at serine 9was significantly lower in tamoxifen-treated breast cancerpatients that eventually had relapses, implying that activationof GSK3? may be associated with the tamoxifen-resistantphenotype. Thus, the combined phosphoproteomeand transcriptome data set analyses revealed distinct signal

Role of Bm30kc6 gene in cell apoptosis and the silk gland degradation signaling pathway in Bombyx mori L.

2020 ◽  
Vol 105 (3) ◽  
Author(s):  
Ying Xiao ◽  
Lei‐lei Li ◽  
Asma Bibi ◽  
Ning Zhang ◽  
Ting Chen ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Silk Gland ◽  
Bombyx Mori L

Sage controls silk gland development by regulating Dfd in Bombyx mori

2021 ◽  
pp. 103568
Author(s):  
Sihan Hou ◽  
Cuicui Tao ◽  
Hongguo Yang ◽  
Tingcai Cheng ◽  
Chun Liu
Keyword(s):  
Bombyx Mori ◽  
Silk Gland ◽  
Gland Development
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