An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
Keyword(s):
The ability to directly measure acetylcholine (ACh) release is an essential first step towards understanding its physiological function. Here we optimized the GRABACh (GPCR-Activation–Based-ACh) sensor with significantly improved sensitivity and minimal downstream coupling. Using this sensor, we measured in-vivo cholinergic activity in both Drosophila and mice, revealing compartmental ACh signals in fly olfactory center and single-trial ACh dynamics in multiple regions of the mice brain under a variety of different behaviors
1991 ◽
Vol 181
(2)
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pp. 524-528
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2001 ◽
Vol 280
(5)
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pp. H2006-H2010
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