scholarly journals CrystalMation: Capacity, reproducibility and efficiency of a fully integrated automatic high-throughput crystallization platform

2007 ◽  
Vol 63 (a1) ◽  
pp. s117-s117 ◽  
Author(s):  
C. Mallett ◽  
M. Mickley ◽  
R. Mosko ◽  
D. Robbins ◽  
C. Sterling ◽  
...  
Keyword(s):  
High Throughput ◽  
Fully Integrated

scholarly journals Fully integrated high-throughput methodology for the study of Ni- and Cu-supported catalysts for glucose hydrogenation

2019 ◽  
Vol 338 ◽  
pp. 72-80 ◽  
Author(s):  
L. Silvester ◽  
F. Ramos ◽  
J. Thuriot-Roukos ◽  
S. Heyte ◽  
M. Araque ◽  
...  
Keyword(s):  
High Throughput ◽  
Supported Catalysts ◽  
Fully Integrated

scholarly journals Instrumental setup for high-throughput small- and wide-angle solution scattering at the X33 beamline of EMBL Hamburg

2012 ◽  
Vol 45 (3) ◽  
pp. 489-495 ◽  
Author(s):  
Clement E. Blanchet ◽  
Alexey V. Zozulya ◽  
Alexey G. Kikhney ◽  
Daniel Franke ◽  
Peter V. Konarev ◽  
...  
Keyword(s):  
High Throughput ◽  
Digital Camera ◽  
Sample Volume ◽  
Wide Angle ◽  
Pixel Detector ◽  
Fully Integrated ◽  
X Ray Scattering ◽  
Angle Scattering ◽  
Rapid Switching ◽  
User Friendly

A setup is presented for automated high-throughput measurements of small-angle X-ray scattering (SAXS) from macromolecular solutions on the bending-magnet beamline X33 of EMBL at the storage ring DORIS-III (DESY, Hamburg). A new multi-cell compartment allows for rapid switching between in-vacuum and in-air operation, for digital camera assisted control of cell filling and for colour sample illumination. The beamline is equipped with a Pilatus 1 M-W pixel detector for SAXS and a Pilatus 300 k-W for wide-angle scattering (WAXS), and results from the use of the Pilatus detectors for scattering studies are reported. The setup provides a broad resolution range from 100 to 0.36 nm without the necessity of changing the sample-to-detector distance. A new optimized robotic sample changer is installed, permitting rapid and reliable automated sample loading and cell cleaning with a required sample volume of 40 µl. All the devices are fully integrated into the beamline control software system, ensuring fully automated and user-friendly operation (attended, unattended and remote) with a throughput of up to 15 measurements per hour.

scholarly journals SERS-based droplet microfluidics for high-throughput gradient analysis

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 674-681 ◽  
Author(s):  
Jinhyeok Jeon ◽  
Namhyun Choi ◽  
Hao Chen ◽  
Joung-Il Moon ◽  
Lingxin Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Gradient Analysis ◽  
Simultaneous Detection ◽  
Droplet Microfluidics ◽  
Fully Integrated

We report a fully integrated SERS-based microdroplet platform for simultaneous detection of various concentrations of a reagent.

scholarly journals A Single-Bead-Based, Fully Integrated Microfluidic System for High-Throughput CD4+T Lymphocyte Enumeration

2017 ◽  
Vol 23 (2) ◽  
pp. 134-143 ◽  
Author(s):  
Xianbo Qiu ◽  
Junhui Zhang ◽  
Shisong Gong ◽  
Dong Wang ◽  
Shan Qiao ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
High Throughput ◽  
Point Of Care ◽  
Low Cost ◽  
Ccd Camera ◽  
Microfluidic System ◽  
Magnetic Control ◽  
Magnetic Actuation ◽  
Fully Integrated ◽  
Single Bead

A single-bead-based, fully integrated microfluidic system has been developed for high-throughput CD4+T lymphocyte enumeration at point-of-care testing. Instead of directly counting CD4+T lymphocytes, CD4+T lymphocyte enumeration is achieved by quantitatively detecting CD4 antigen from the lysed blood sample with a functionalized polycarbonate single bead based on chemiluminescence. To implement the sandwiched chemiluminescence immunoassay with reduced nonspecific binding, a streamlined microfluidic chip with multiple reaction chambers is developed to allow each reaction step to be completed in an independent chamber where reagent is pre-stored. With simple magnetic control, the single bead with an embedded ferrous core can be consecutively transported between each of two adjacent chambers for different reactions. Meanwhile, enhanced mixing can be achieved by moving the single bead back and forth inside one chamber with magnetic actuation. High-throughput detection can be performed when a linear actuation stage is adopted to introduce synchronous magnetic control to multiple single beads in parallel microfluidic chips. A sensitive charge-coupled device (CCD) camera is adopted for high-throughput chemiluminescence detection from multiple single beads. Experimental results show that with the fully integrated microfluidic system, easy-to-operate, accurate, low-cost, immediate, and high-throughput CD4+T lymphocyte enumeration can be successfully achieved at resource-poor settings.

INSIDE INDUSTRY

2016 ◽  
Vol 20 (07) ◽  
pp. 44-52
Keyword(s):  
Drinking Water ◽  
Health System ◽  
High Throughput ◽  
Safe Drinking Water ◽  
Health Index ◽  
Antimalarial Treatment ◽  
Future Health ◽  
Fully Integrated ◽  
Dengue Prevention

P&G Children's Safe Drinking Water Program Celebrates 10 Billion Litre Milestone in Singapore. New In-Vitro Studies Reinforce Efficacy of BETADINE® Skin Cleanser against Viruses that Cause Hand Foot & Mouth Disease. Singapore Ranked Fifth in its Readiness to Achieve a Fully Integrated Health System Reveals First Future Health Index. SCIEX Announces High Throughput, Industrialised Omics Solutions. Novartis Expands Partnership with Medicines for Malaria Venture to Develop Next-Generation Antimalarial Treatment. ADVA Launches Dengue Mission Buzz Barometer Tool to Boost Critical Dengue Prevention Awareness and Preparedness Amongst ASEAN Community. Innovative Diagnostics Awarded Unprecedented Westgard Sigma Certification in Singapore.

scholarly journals Analytical Evaluation of the Ideal Strategy for High-Throughput Flow Injection Analysis by Tandem Mass Spectrometry in Routine Newborn Screening

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 473
Author(s):  
Ilaria Cicalini ◽  
Silvia Valentinuzzi ◽  
Damiana Pieragostino ◽  
Ada Consalvo ◽  
Mirco Zucchelli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Newborn Screening ◽  
High Throughput ◽  
Flow Injection ◽  
Tandem Mass ◽  
Fully Integrated ◽  
System A ◽  
Sample Extract ◽  
Expanded Newborn Screening

The introduction of tandem mass spectrometry (MS/MS) to clinical laboratories and the advent of expanded newborn screening (NBS) were crucial changes to public health programs worldwide. Speed, robustness, accuracy, selectivity, and specificity of analysis are all requirements of expanded NBS and are needed to minimize false positive results risks, to possibly eliminate false negatives, and to improve the positive predictive value of NBS. In this study, we firstly evaluated the analytical performances of the RenataDX Screening System, a fully integrated flow-injection MS/MS (FIA-MS/MS) IVD system for high-throughput dried blood spot (DBS) analysis in a routine NBS laboratory. Since a choice of several commercial NBS kits is available, we sought to compare NeoBaseTM 2 (PerkinElmer®) and MassChrom® (Chromsystems) non-derivatized kits on the RenataDX platform by evaluating their analytical performances. Moreover, we verified the degree of correlation between data obtained by the two different NBS MS/MS kits by FIA-MS/MS of over 500 samples. Our data suggest that both methods correlate well with clinically insignificant differences that do not impact the NBS result. Finally, while NeoBase™ 2 offers an easier and faster sample preparation, MassChrom® provides a cleaner sample extract which empirically should improve instrument reliability.

scholarly journals High Throughput Screening Using Fully Integrated Robotic Screening

1997 ◽  
Vol 2 (3) ◽  
pp. 133-135 ◽  
Author(s):  
Martyn Banks ◽  
Alastair Binnie ◽  
Simon Fogarty
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Fully Integrated

scholarly journals Cyto-Mine: An Integrated, Picodroplet System for High-Throughput Single-Cell Analysis, Sorting, Dispensing, and Monoclonality Assurance

2020 ◽  
Vol 25 (2) ◽  
pp. 177-189 ◽  
Author(s):  
Dimitris Josephides ◽  
Serena Davoli ◽  
William Whitley ◽  
Raphael Ruis ◽  
Robert Salter ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Line ◽  
High Throughput ◽  
Cell Sorting ◽  
High Throughput Screening ◽  
Single Cell Analysis ◽  
Microtiter Plate ◽  
Cell Analysis ◽  
Fully Integrated ◽  
Clonal Cell Lines

The primary goal of bioprocess cell line development is to obtain high product yields from robustly growing and well-defined clonal cell lines in timelines measured in weeks rather than months. Likewise, high-throughput screening of B cells and hybridomas is required for most cell line engineering workflows. A substantial bottleneck in these processes is detecting and isolating rare clonal cells with the required characteristics. Traditionally, this was achieved by the resource-intensive method of limiting dilution cloning, and more recently aided by semiautomated technologies such as cell sorting (e.g., fluorescence-activated cell sorting) and colony picking. In this paper we report on our novel Cyto-Mine Single Cell Analysis and Monoclonality Assurance System, which overcomes the limitations of current technologies by screening hundreds of thousands of individual cells for secreted target proteins, and then isolating and dispensing the highest producers into microtiter plate wells (MTP). The Cyto-Mine system performs this workflow using a fully integrated, microfluidic Cyto-Cartridge. Critically, all reagents and Cyto-Cartridges used are animal component-free (ACF) and sterile, thus allowing fast, robust, and safe isolation of desired cells.

scholarly journals High-Throughput Screening: Update on Practices and Success

2006 ◽  
Vol 11 (7) ◽  
pp. 864-869 ◽  
Author(s):  
Sandra Fox ◽  
Shauna Farr-Jones ◽  
Lynne Sopchak ◽  
Amy Boggs ◽  
Helen Wang Nicely ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
New Technologies ◽  
Assay Development ◽  
Target Validation ◽  
Fully Integrated ◽  
Drug Candidates ◽  
Secondary Screening ◽  
Biotechnology Companies

High-throughput screening (HTS) has become an important part of drug discovery at most pharmaceutical and many biotechnology companies worldwide, and use of HTS technologies is expanding into new areas. Target validation, assay development, secondary screening, ADME/Tox, and lead optimization are among the areas in which there is an increasing use of HTS technologies. It is becoming fully integrated within drug discovery, both upstream and downstream, which includes increasing use of cell-based assays and high-content screening (HCS) technologies to achieve more physiologically relevant results and to find higher quality leads. In addition, HTS laboratories are continually evaluating new technologies as they struggle to increase their success rate for finding drug candidates. The material in this article is based on a 900-page HTS industry report involving 54 HTS directors representing 58 HTS laboratories and 34 suppliers.

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