Detection of MGMT methylation status using a Lab-on-Chip compatible isothermal amplification method

Author(s):  
Myesha Jahin ◽  
Benji Fenech-Salerno ◽  
Nicolas Moser ◽  
Pantelis Georgiou ◽  
James Flanagan ◽  
...  
2008 ◽  
Vol 01 (02) ◽  
pp. 257-265 ◽  
Author(s):  
GUOLIANG HUANG ◽  
XIAOYONG YANG ◽  
JIANG ZHU ◽  
SHUKUAN XU ◽  
CHENG DENG ◽  
...  

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method. Compared with the widely utilized polymerase chain reaction (PCR), LAMP has higher speed and efficiency as well as lower requirement for system temperature control because the whole amplification process is isothermal and no efforts are needed to switch between different temperatures. In this paper, we designed and fabricated different kinds of polycarbonate (PC) microfluid chips, explored appropriate reaction condition for LAMP in microenvironment (1 nL → 10 μL), and developed a microfluidic isothermal amplification detection system. The DNA optimal amplification temperature is obtained; the starting time of exponential amplification of DNA is put forward farther. The optimal condition of DNA amplification in microenvironment, with a little reaction materials and early starting exponential amplification time of DNA are very important for clinic DNA detection and the application of Lab-on-a-Chip.


Lab on a Chip ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 2296-2305 ◽  
Author(s):  
Siyuan Wang ◽  
Ning Liu ◽  
Lingyan Zheng ◽  
Gaozhe Cai ◽  
Jianhan Lin

Rapid screening of foodborne pathogens is key to prevent food poisoning.


2021 ◽  
pp. 2004101
Author(s):  
Marco Giacometti ◽  
Francesca Milesi ◽  
Pietro Lorenzo Coppadoro ◽  
Alberto Rizzo ◽  
Federico Fagiani ◽  
...  
Keyword(s):  

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1113
Author(s):  
Mohammed Asadullah Khan ◽  
Jürgen Kosel

An integrated polymer-based magnetohydrodynamic (MHD) pump that can actuate saline fluids in closed-channel devices is presented. MHD pumps are attractive for lab-on-chip applications, due to their ability to provide high propulsive force without any moving parts. Unlike other MHD devices, a high level of integration is demonstrated by incorporating both laser-induced graphene (LIG) electrodes as well as a NdFeB magnetic-flux source in the NdFeB-polydimethylsiloxane permanent magnetic composite substrate. The effects of transferring the LIG film from polyimide to the magnetic composite substrate were studied. Operation of the integrated magneto hydrodynamic pump without disruptive bubbles was achieved. In the studied case, the pump produces a flow rate of 28.1 µL/min. while consuming ~1 mW power.


Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ali Rostamian ◽  
Ehsan Madadi-Kandjani ◽  
Hamed Dalir ◽  
Volker J. Sorger ◽  
Ray T. Chen

Abstract Thanks to the unique molecular fingerprints in the mid-infrared spectral region, absorption spectroscopy in this regime has attracted widespread attention in recent years. Contrary to commercially available infrared spectrometers, which are limited by being bulky and cost-intensive, laboratory-on-chip infrared spectrometers can offer sensor advancements including raw sensing performance in addition to use such as enhanced portability. Several platforms have been proposed in the past for on-chip ethanol detection. However, selective sensing with high sensitivity at room temperature has remained a challenge. Here, we experimentally demonstrate an on-chip ethyl alcohol sensor based on a holey photonic crystal waveguide on silicon on insulator-based photonics sensing platform offering an enhanced photoabsorption thus improving sensitivity. This is achieved by designing and engineering an optical slow-light mode with a high group-index of n g  = 73 and a strong localization of modal power in analyte, enabled by the photonic crystal waveguide structure. This approach includes a codesign paradigm that uniquely features an increased effective path length traversed by the guided wave through the to-be-sensed gas analyte. This PIC-based lab-on-chip sensor is exemplary, spectrally designed to operate at the center wavelength of 3.4 μm to match the peak absorbance for ethanol. However, the slow-light enhancement concept is universal offering to cover a wide design-window and spectral ranges towards sensing a plurality of gas species. Using the holey photonic crystal waveguide, we demonstrate the capability of achieving parts per billion levels of gas detection precision. High sensitivity combined with tailorable spectral range along with a compact form-factor enables a new class of portable photonic sensor platforms when combined with integrated with quantum cascade laser and detectors.


2021 ◽  
Vol 23 (2) ◽  
Author(s):  
Subhan Shaik ◽  
Aarthi Saminathan ◽  
Deepak Sharma ◽  
Jagdish A Krishnaswamy ◽  
D Roy Mahapatra

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 65
Author(s):  
Puneet Manocha ◽  
Gitanjali Chandwani

Molecular communication is a bioinspired communication that enables macro-scale, micro-scale and nano-scale devices to communicate with each other. The molecular communication system is prone to severe signal attenuation, dispersion and delay, which leads to performance degradation as the distance between two communicating devices increases. To mitigate these challenges, relays are used to establish reliable communication in microfluidic channels. Relay assisted molecular communication systems can also enable interconnection among various entities of the lab-on-chip for sharing information. Various relaying schemes have been proposed for reliable molecular communication systems, most of which lack practical feasibility. Thus, it is essential to design and develop relays that can be practically incorporated into the microfluidic channel. This paper presents a novel design of passive in-line relay for molecular communication system that can be easily embedded in the microfluidic channel and operate without external energy. Results show that geometric modification in the microfluidic channel can act as a relay and restore the degraded signal up-to 28%.


2021 ◽  
Vol 8 (14) ◽  
pp. 2170087
Author(s):  
Marco Giacometti ◽  
Francesca Milesi ◽  
Pietro Lorenzo Coppadoro ◽  
Alberto Rizzo ◽  
Federico Fagiani ◽  
...  
Keyword(s):  

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