Detection of Lung Cancer with Phase-Contrast X-ray Imaging Using Synchrotron Radiation

For the extraction of the best possible X-ray diffraction data from macromolecular crystals, accurate positioning of the crystals with respect to the X-ray beam is crucial. In addition, information about the shape and internal defects of crystals allows the optimization of data-collection strategies. Here, it is demonstrated that the X-ray beam available on the macromolecular crystallography beamline P14 at the high-brilliance synchrotron-radiation source PETRA III at DESY, Hamburg, Germany can be used for high-energy phase-contrast microtomography of protein crystals mounted in an optically opaque lipidic cubic phase matrix. Three-dimensional tomograms have been obtained at X-ray doses that are substantially smaller and on time scales that are substantially shorter than those used for diffraction-scanning approaches that display protein crystals at micrometre resolution. Adding a compound refractive lens as an objective to the imaging setup, two-dimensional imaging at sub-micrometre resolution has been achieved. All experiments were performed on a standard macromolecular crystallography beamline and are compatible with standard diffraction data-collection workflows and apparatus. Phase-contrast X-ray imaging of macromolecular crystals could find wide application at existing and upcoming low-emittance synchrotron-radiation sources.

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Medical Applications with Synchrotron Radiation in Japan

Journal of Synchrotron Radiation ◽

10.1107/s0909049597018682 ◽

1998 ◽

Vol 5 (3) ◽

pp. 326-332 ◽

Cited By ~ 11

Author(s):

Tohoru Takeda ◽

Yuji Itai ◽

Kazuyuki Hyodo ◽

Masami Ando ◽

Takao Akatsuka ◽

...

Keyword(s):

Synchrotron Radiation ◽

Coronary Arteries ◽

Phase Contrast ◽

Coronary Arteriography ◽

Medical Applications ◽

Imaging Method ◽

Two Dimensional ◽

X Ray ◽

X Ray Imaging ◽

X Ray Computed

In Japan, various medical applications of synchrotron X-ray imaging, such as angiography, monochromatic X-ray computed tomography (CT), radiography and radiation therapy, are being developed. In particular, coronary arteriography (CAG) is quite an important clinical application of synchrotron radiation. Using a two-dimensional imaging method, the first human intravenous CAG was carried out at KEK in May 1996; however, further improvements of image quality are required in clinical practice. On the other hand, two-dimensional aortographic CAG revealed canine coronary arteries as clearly as those on selective CAG, and coronary arteries less than 0.2 mm in diameter. Among applications of synchrotron radiation to X-ray CT, phase-contrast X-ray CT and fluorescent X-ray CT are expected to be very interesting future applications of synchrotron radiation. For actual clinical applications of synchrotron radiation, a medical beamline and a laboratory are now being constructed at SPring-8 in Harima.

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Experience with Imaging by Using of Microfocus X-Ray Source

Journal of Electrical Engineering ◽

10.2478/v10187-010-0042-3 ◽

2010 ◽

Vol 61 (5) ◽

pp. 287-290 ◽

Cited By ~ 2

Author(s):

Zdenko Zápražný ◽

Dušan Korytár ◽

František Dubecký ◽

Vladimír Áč ◽

Zbigniew Stachura ◽

...

Keyword(s):

Synchrotron Radiation ◽

Phase Contrast ◽

Ccd Camera ◽

X Ray ◽

X Ray Imaging ◽

Radiation Sources ◽

Set Up ◽

High Level ◽

Very High

Experience with Imaging by Using of Microfocus X-Ray SourceIn this paper we present the current work and experience with using microfocus x-ray generator and commercial CCD camera for x-ray imaging purpose. There is a need in laboratories for the development of imaging methods approaching synchrotron radiation sources, where the brilliance of radiation is on very high-level. Generally, there is no continuous access to synchrotron facilities. Several synchrotron radiation laboratories allocate the access via a proposal system. Thus the time for synchrotron radiation experiments seldom exceeds more than 1-2 weeks per year, which restricts its application to a few selected experiments. Even in future, the routine characterization of samples will be performed mainly at the experimenters home laboratories [10]. In this contribution we show that with the present set-up it is possible to achieve the spatial resolution down to μm and with the appropriate geometry a phase contrast images are observable.

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Faculty Opinions recommendation of Performance of ultralow-dose CT with iterative reconstruction in lung cancer screening: limiting radiation exposure to the equivalent of conventional chest X-ray imaging.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726104848.793525333 ◽

2016 ◽

Author(s):

Stefan Bielack

Keyword(s):

Lung Cancer ◽

Cancer Screening ◽

Radiation Exposure ◽

Iterative Reconstruction ◽

Lung Cancer Screening ◽

Ultralow Dose ◽

X Ray ◽

X Ray Imaging ◽

Chest X Ray

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Principles of Different X-ray Phase-Contrast Imaging: A Review

Applied Sciences ◽

10.3390/app11072971 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2971

Author(s):

Siwei Tao ◽

Congxiao He ◽

Xiang Hao ◽

Cuifang Kuang ◽

Xu Liu

Keyword(s):

Biological Samples ◽

Phase Contrast ◽

Recent Progress ◽

Phase Contrast Imaging ◽

Contrast Imaging ◽

X Ray ◽

X Ray Imaging ◽

Internal Structures ◽

X Ray Absorption ◽

Made In

Numerous advances have been made in X-ray technology in recent years. X-ray imaging plays an important role in the nondestructive exploration of the internal structures of objects. However, the contrast of X-ray absorption images remains low, especially for materials with low atomic numbers, such as biological samples. X-ray phase-contrast images have an intrinsically higher contrast than absorption images. In this review, the principles, milestones, and recent progress of X-ray phase-contrast imaging methods are demonstrated. In addition, prospective applications are presented.

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