Investigation of Fungus Growth and Moss Growth on Different Wall Care Putty Materials Along with Different Walling Materials

Author(s):  
S. A. A. Gunawardanaa ◽  
G.A.H.H. Galkanda ◽  
R. U. Halwatura
Keyword(s):  
2014 ◽  
Vol 1000 ◽  
pp. 154-157
Author(s):  
Zuzana Rácová ◽  
Petra Hrochová ◽  
Pavla Ryparová

Dry rot fungus (Serpula lacrymans) is wood-decaying fungus. It grows frequently in our territory and it causes big damages on structures. Remediation of damaged structures is very difficult, sometimes impossible, therefore it is necessary to study preventive protection against dry rot fungus. PVA nanofibred fabrics with synthetic and natural biocidal additives were used for this experiment. Filter papers soaked in dopes with biocidal substances were other materials used for this experiment. Pieces of nanofiber fabrics and pieces of filter papers soaked in dopes were placed to Petri dishes with broth. Small cuts of dry rot fungus were placed around them. This experiment was performed in conditions, which promote the growth of dry rot fungus. Growth of dry rot fungus was studied.


Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 143
Author(s):  
Neringa Rasiukevičiūtė ◽  
Aušra Brazaitytė ◽  
Viktorija Vaštakaitė-Kairienė ◽  
Alma Valiuškaitė

The study aimed to evaluate the effect of different photon flux density (PFD) and light-emitting diodes (LED) wavelengths on strawberry Colletotrichum acutatum growth characteristics. The C. acutatum growth characteristics under the blue 450 nm (B), green 530 nm (G), red 660 nm (R), far-red 735 nm (FR), and white 5700 K (W) LEDs at PFD 50, 100 and 200 μmol m−2 s−1 were evaluated. The effect on C. acutatum mycelial growth evaluated by daily measuring until five days after inoculation (DAI). The presence of conidia and size (width and length) evaluated after 5 DAI. The results showed that the highest inhibition of fungus growth was achieved after 1 DAI under B and G at 50 μmol m−2 s−1 PFD. Additionally, after 1–4 DAI under B at 200 μmol m−2 s−1 PFD. The lowest conidia width was under FR at 50 μmol m−2 s−1 PFD and length under FR at 100 μmol m−2 s−1 PFD. Various LED light wavelengths influenced differences in C. acutatum colonies color. In conclusion, different photosynthetic photon flux densities and wavelengths influence C. acutatum growth characteristics. The changes in C. acutatum morphological and phenotypical characteristics could be related to its ability to spread and infect plant tissues. This study’s findings could potentially help to manage C. acutatum by LEDs in controlled environment conditions.


Author(s):  
Rini Yanti ◽  
Hermina Nurdiawati ◽  
Puji Wulandari ◽  
Yudi Pranoto ◽  
Muhammad Nur Cahyanto

Turmeric rhizomes are commonly used in the culinary, pharmaceutical, herbal medicine, and beverage industries. On the contrary, turmeric leaves are underutilized.  The aims of this study were to extract the essential oil from turmeric leaves, characterize the chemical composition of the oil, and determine its antifungal activities against aflatoxin-producing fungi. Steam distillation was used to extract the essential oil from turmeric leaves. The properties of the oil were identified using GC-MS. Antimicrobial activities against Aspergillus flavus and Aspergillus parasiticus were determined. Spores of the fungi were inoculated into potato dextrose agar plates supplemented with various quantities of turmeric leaves essential oil and incubated at 30°C for 7 days. The oil's primary constituents were α-phelandrene(46.70 %), followed by α-terpinolene (17.39 %), 1,8-cineole (8.78 %), benzene (4.24 %), and 2-β pinene (3.64 %). At low (<1%) concentrations, the oil delayed mycelia formation and at high concentrations it significantly inhibit fungal growth (at 1%) and completely inhibit colony formation (at 2%) Additionally, the result show that turmeric leaves oil can inhibited fungus growth at the lowest concentration (0.25 %) when compared to the control over a seven-day incubation period.


2021 ◽  
Vol 9 (2) ◽  
pp. 269
Author(s):  
Rizka Diah Permana ◽  
Hasan Ashari Oramahi ◽  
Farah Diba

The efficacy of liquid smoke obtained from medang wood (Cinnamomum sp.)against Schizophyllum commune fungus was evaluated. This study aims to evaluate the antifungal properties of liquid smoke from medang against S. commune fungus. Potato Dextrose Agar (PDA) medium was used to determine the efficacy of the liquid smoke of medang wood on S. commune fungus growth. Three kinds of liquid smoke were obtained from the pyrolysis of medang wood at 370, 400, and 430°C. The efficacy of liquid smoke from medang wood for antifungal is a factorial 3 by 4 in a completely randomized design; the first factor was pyrolysis temperature of liquid smoke from medang (370, 400, and 430°C), and the second factor was the treatment of concentration of liquid smoke from medang wood (0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0%, v/v). The results showed that pyrolysis temperature affected liquid smoke from medang, and the concentration of liquid smoke was significantly different for inhibition of fungal growth. The results indicated that the pyrolysis temperature of liquid smoke production and the concentration of liquid smoke had a significant effect on S. commune fungus growth inhibition. Medang wood liquid smoke effectively inhibited the growth of S. commune fungus about 98.57% at a concentration of 2.5% with liquid smoke pyrolysis temperature used is 430°C.Keywords: efficacy, inhibition of fungal growth, liquid smoke, medang wood, Schizophyllum commune


1962 ◽  
Vol 42 (2) ◽  
pp. 302-307 ◽  
Author(s):  
N. J. Whitney ◽  
C. G. Mortimore

In bio-assay tests ether extracts of the roots and of the stalks of both resistant and susceptible corn inbreds inhibited the growth of Fusarium moniliforme Sheld. and Gibberella zeae (Schw.) Petch, the level of inhibition varying over the season. However, the extracts of the resistant and susceptible inbreds did not differ consistently in their ability to inhibit fungus growth on any given date. Thus, this bio-assay method cannot be used to evaluate inbreds for resistance to root and stalk rot.


2013 ◽  
Vol 43 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Rosane Fátima Baldiga Tonin ◽  
Aveline Avozani ◽  
Anderson Luiz Durante Danelli ◽  
Erlei Melo Reis ◽  
Sandra Maria Zoldan ◽  
...  

Black root rot, caused by Macrophomina phaseolina (Tass.) Goid., is the most common root disease in soybean fields. This study aimed to determine the in vitro mycelial sensitivity, measured by the IC50 (concentration to inhibit 50% of the fungus mycelial growth) of a M. phaseolina isolate obtained from soybean, to different fungicides (thiram, iprodione, carbendazim, pyraclostrobin, fluquinconazol, tolyfluanid, metalaxyl and penflufen + trifloxystrobin), at six concentrations (0.01 mg L-1, 0.10 mg L-1, 1.00 mg L-1, 10.00 mg L-1, 20.00 mg L-1 and 40.00 mg L-1 of the active ingredient). The 0.00 mg L-1 concentration represented the control, without fungicide addition. The mycelial growth evaluation was performed with the aid of a digital pachymeter, by measuring the colonies diameter, when the fungus growth in the control treatment reached the Petri dish edge. The experimental design was completely randomized, with four replications. Concerning the fungitoxicity of active ingredients, a variation from non-toxic to highly fungitoxic was observed to the M. phaseolina isolate, with IC50 values ranging from 0.23 mg L-1 to > 40.00 mg L-1, being carbendazim the most efficient one (IC50 = 0.23 mg L-1). The fungus showed insensitivity to the active ingredients of fluquinconazole, metalaxyl, thiram and tolyfluanid.


2020 ◽  
Vol 6 (4) ◽  
pp. 342
Author(s):  
Rostislav Zemek ◽  
Jana Konopická ◽  
Zain Ul Abdin

Buxus sp. is an important native and ornamental tree in Europe threatened by a serious invasive pest Cydalima perspectalis. The larvae of this moth are able to defoliate box trees and cause their death. The development of novel biopesticides targeting this pest might help protect Buxus trees grown wildly or in city parks. Laboratory experiments were conducted to assess the efficacy of entomopathogenic fungus Isaria fumosorosea strain CCM 8367 against C. perspectalis. The last-instar larvae of the box tree moth were treated by the suspension of fungus conidia at concentrations ranging from 1 × 104 to 1 × 108 spores per 1 mL. Fungus infection was observed mostly in pupae, but the maximum mortality did not exceed 60%, indicating a very low susceptibility of C. perspectalis to I. fumosorosea. Furthermore, a number of ungerminated fungal conidia were found on larval cuticles using a low-temperature scanning electron microscopy. Our data also reveal that the hydroalcoholic extract from B. sempervirens leaves significantly inhibits both the germination of I. fumosorosea conidia and fungus growth. It can be speculated that the strain CCM 8367 of I. fumosorosea is not a potent biocontrol agent against C. perspectalis and low virulence of the fungus might be due to the accumulation of host plant phytochemicals having antimicrobial activity in larval cuticle of the pest.


2020 ◽  
Vol 80 (2) ◽  
pp. 460-464 ◽  
Author(s):  
A. L. B. Dias ◽  
W. C. Sousa ◽  
H. R. F. Batista ◽  
C. C. F. Alves ◽  
E. L. Souchie ◽  
...  

Abstract Essential oils (EO) from aromatic and medicinal plants generally perform a diverse range of biological activities because they have several active constituents that work in different mechanisms of action. EO from Citrus peel have an impressive range of food and medicinal uses, besides other applications. EO from Citrus reticulata, C. sinensis and C. deliciosa were extracted from fruit peel and analyzed by GC-MS. The major constituent of EO under evaluation was limonene, whose concentrations were 98.54%, 91.65% and 91.27% for C. sinensis, C. reticulata and C. deliciosa, respectively. The highest potential of inhibition of mycelial growth was observed when the oil dose was 300 μL. Citrus oils inhibited fungus growth in 82.91% (C. deliciosa), 65.82% (C. sinensis) and 63.46% (C. reticulata). Anti-Sclerotinia sclerotiorum activity of 90% pure limonene and at different doses (20, 50, 100, 200 and 300 μL) was also investigated. This monoterpene showed to be highly active by inhibiting 100% fungus growth even at 200 and 300 μL doses. This is the first report of the in vitro inhibitory effect of natural products from these three Citrus species and its results show that there is good prospect of using them experimentally to control S. sclerotiorum, in both greenhouse and field conditions.


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