This paper describes a new concept to monitor the temperature of water utilizing the acoustic resonance, which occurs when ultrasound passes through a thin layer. In the ultrasonic transmission system that comprises of the reflection plate, thin film, and water, the reflection coefficient of the ultrasound at the plate/film/water interface depends on the frequency and takes its minimum value at the resonant frequency. Notably, this is closely related to the acoustic impedance of the water; moreover, it is a known fact that the acoustic impedance of the water demonstrates temperature dependence. Against this background, the present study aims to develop a technique in order to monitor the temperature of water utilizing the aforementioned correlation between the reflection coefficient and water temperature. First, a theoretical model was developed to determine the acoustic impedance of water from the difference in the amplitude spectra of echoes reflected at the back of the plate in the cases both with and without the film. It was found that the ratio of the amplitude spectrum of the echo recorded in the case with the film to that in the case without the film clearly decreased with a drop in water temperature. From this, we obtained the equation for determining water temperature experimentally. Finally, the temperature of water, which was brought down by air or ice cooling, was monitored by the proposed method. It was found that the behavior of temperature determined by the proposed method was congruent with that which was measured by a thermocouple.