Replacing fish oil with palm oil: Effects on mRNA expression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia ( Oreochromis niloticus )

2018 ◽  
Vol 24 (6) ◽  
pp. 1822-1833 ◽  
Author(s):  
Christian Larbi Ayisi ◽  
Jin‐Liang Zhao ◽  
Xue‐Ming Hua ◽  
Andrews Apraku
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Fish Oil ◽  
Mrna Expression ◽  
Palm Oil ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Fatty Acid Transport ◽  
Acid Transport ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 28
Author(s):  
Min Tian ◽  
Jiaming Chen ◽  
Zhihui Wu ◽  
Hanqing Song ◽  
Fei Yang ◽  
...  
Keyword(s):  
Small Intestine ◽  
Fatty Acid ◽  
Soybean Oil ◽  
Mrna Expression ◽  
Transport System ◽  
Palm Oil ◽  
Control Group ◽  
Fatty Acid Transport ◽  
Acid Transport ◽  
Fatty Acid Binding

(1) Background: Nutritional strategies to enhance gut function and reduce the piglet diarrhea rate are critical to increase the growth performance of piglets. The purpose of this study was to investigate whether dietary fat types and/or fat microencapsulation techniques are involved in regulating the fatty acid transport system and the mechanical and immunological barriers of the small intestine. (2) Methods: Three hundred twenty-four weaning piglets were randomly divided into three groups fed a soybean oil diet (SBO, control group, 6.0% soybean oil), palm oil diet (PO, 6.0% palm oil), or encapsulated palm oil diet (EPO, 7.5% encapsulated palm oil). (3) Results: A significantly lower mRNA expression of the claudin was observed in the duodenum and jejunum of the PO group than in the SBO group (p < 0.05). However, the mRNA expression and protein abundance of claudin and ZO-1 in the jejunum of the EPO group were higher (p < 0.05) than in the PO group. Porcine β-defensin (pBD) secretion was not significantly different between the SBO and PO groups (p > 0.05), while the pBD-2 levels were significantly different (p < 0.05). Compared with the PO group, the EPO group exhibited a significantly increased secretion of pBD-2 and pBD-129 in the small intestine (p < 0.05) and pBD-1 in the jejunum and ileum (p < 0.05). The protein abundances of apolipoprotein AIV (Apo AIV) and intestinal fatty acid binding protein (I-FABP) were significantly lower in the PO group than in the SBO group (p < 0.05). Simultaneously, the protein abundances of fatty acid transport protein 4 (FATP4), fatty acid translocase (CD36), and I-FABP were higher in the EPO group than in the PO group. Furthermore, the low digestibility of palm oil (PO group) might negatively regulate intestinal tight junctions, fatty acid transporters, lipoproteins, and β-defensin through the activation of the AMPK/mTORC1 and AMPK/Sirt1/NF-κB pathways. (4) Conclusions: In summary, microencapsulation techniques might alleviate the negative effects of palm oil and help to improve the intestinal fatty acid transport system and barrier function.

scholarly journals Effects of replacing fish oil with palm oil in diets of Nile tilapia (Oreochromis niloticus) on muscle biochemical composition, enzyme activities, and mRNA expression of growth-related genes

2019 ◽  
Vol 22 (1) ◽  
Author(s):  
Christian Larbi Ayisi ◽  
Jinliang Zhao ◽  
Chen Yame ◽  
Andrews Apraku ◽  
Grace Debra
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Mrna Expression ◽  
Protein Content ◽  
Palm Oil ◽  
Oreochromis Niloticus ◽  
Enzyme Activities ◽  
Muscle Protein ◽  
Crude Lipid ◽  
Significant Difference

Abstract Background Due to the continuous demand for fish coupled with decline in capture fisheries, there is the need to increase aquaculture production to meet the demand. Aquaculture is faced with high cost of feeding since fish oil and fish meal are expensive. In view of this, there are calls to explore alternatives that are cheap and reliable. Objectives This study on Oreochromis niloticus was conducted to evaluate the effects of replacing fish oil (FO) with palm oil (PO) at 0%, 25%, 50%, 75%, and 100% on muscle fatty acid and proximate composition as well as growth-related enzyme activities and mRNA expression. Methods Oreochromis niloticus were fed five experimental diets (33% crude protein and 10% crude lipid) for 8 weeks. Feed had variation in fish oil and palm oil contents. After the 8 weeks feeding trial, five fish were sampled from each tank (15 from each treatment) and euthanized using an excess dose of tricaine methane sulfonate (MS-222 at 200 mg/L). Fatty acid and enzyme activities were analyzed using standard protocols. Also, RT-qPCR was used to quantify the expression levels of selected growth-related genes. Results Fish fed 25% PO recorded the least muscle protein content and was significantly lower than the group fed 100% PO. Paired box protein 7 (Pax-7) enzyme activity was significantly higher in the group fed 50% PO compared to the groups fed 25% PO and 100% PO, while caplain-3 (Capn-3) was significantly lower in the group fed 0% PO compared to all other groups. There was a significant difference among treatments with respect to mRNA expression of Pax-7 and Capn-3. Group fed 25% PO had significantly lower mRNA expression of Pax-7, while the group fed 75% PO recorded significantly higher mRNA expression of Capn-3 compared to groups fed 0% PO, 25% PO, and 100% PO. Pearson’s correlation analysis revealed that Igf-I and Igf-II mRNA expression have significant correlation with n-3 polyunsaturated fatty acids content in muscle. Conclusion The results suggest muscle protein content could be modified if FO is replaced with PO. Also, mRNA expression of Pax-7 and Capn-3 is affected by replacing FO with PO.

scholarly journals Towards Sustainable Aquafeeds: Complete Substitution of Fish Oil with Marine Microalga Schizochytrium sp. Improves Growth and Fatty Acid Deposition in Juvenile Nile Tilapia (Oreochromis niloticus)

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0156684 ◽  
Author(s):  
Pallab K. Sarker ◽  
Anne R. Kapuscinski ◽  
Alison J. Lanois ◽  
Erin D. Livesey ◽  
Katie P. Bernhard ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Acid Deposition ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Marine Microalga ◽  
Nile Tilapia Oreochromis Niloticus
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