Effect of cell‐free spent media prepared from Aggregatibacter actinomycetemcomitans on the growth of Candida albicans and Streptococcus mutans in co‐species biofilms

2020 ◽  
Vol 128 (5) ◽  
pp. 395-404 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Spent Media

scholarly journals Evaluación de la actividad antimicrobiana de los dentífricos basados en propóleos en patógenos orales

2020 ◽  
Vol 33 (2) ◽  
pp. 12-22
Author(s):  
Tatiana Cerveira Valois de Sá ◽  
Valerio Monteiro-Neto ◽  
Cadidja Dayane Sousa do Carmo ◽  
Cecilia Claudia Costa Ribeiro ◽  
Claudia Maria Coelho Alves
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Lactobacillus Acidophilus ◽  
Fusobacterium Nucleatum ◽  
Aggregatibacter Actinomycetemcomitans

Introducción y objetivo: La incorporación de propóleos en los dentífricos tiene como objetivo ayudar de manera más efectiva al control y la prevención de patologías orales a través de la eliminación de los patógenos presentes en la biopelícula. Sin embargo, se sabe poco sobre la eficacia antimicrobiana de diferentes productos en el mercado de microorganismos para estas patologías. El objetivo de este estudio fue investigar la acción antimicrobiana de tres dentífricos que contienen propóleos sobre los patógenos orales. Material y métodos: Se utilizó el método de difusión en agar para analizar tres dentífricos basados en propóleos, que incluyen: Noplak Max®, Protta® y Forever Bright®. Se utilizó un dentífrico sin propóleos (Malvatrikids ®) como control negativo. Los controles positivos fueron 0,2% de clorhexidina diluida adicionalmente al 30% para igualar la concentración de clorhexidina de uno de los dentífricos evaluados, y el extracto de propóleos (Apis Flora®) al 11%. Para la determinación de la actividad antimicrobiana se utilizaron las cepas de Fusobacterium nucleatum y Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, Streptococcus mutans, Lactobacillus acidophilus y Candida albicans. Resultados: De los dentífricos probados, Protta® y Forever Bright® mostraron acción inhibitoria contra S. mutans, E. faecalis y microorganismos de C. albicans. El dentífrico Noplak® mostró baja actividad antimicrobiana, limitándose a S. mutans y E. faecalis. Cuando hubo un efecto antimicrobiano, los diámetros de los halos de inhibición del crecimiento variaron de 9mm a 28,83mm. Conclusión: El uso de un dentífrico que contiene propóleos para su uso eventual como complemento terapéutico en odontología está justificado, considerando las actividades farmacológicas.

scholarly journals Cell Toxicity and inhibitory effects of Cyperus rotundus extract on Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Candida albicans

2018 ◽  
Vol 28 (4) ◽  
Author(s):  
Moein Khojaste ◽  
Mohsen Yazdanian ◽  
Elahe Tahmasebi ◽  
Mehdi Shokri ◽  
Behzad Houshmand ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Antimicrobial Effect ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Cyperus Rotundus ◽  
Tooth Decay ◽  
Gingival Fibroblast ◽  
Alcoholic Extract ◽  
Aqueous Extracts

Periodontitis and tooth decay are common dental diseases. There are some bacterial risk factors such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Candida albicans complications, tubers of Cyperus rotundus was used to determine the antimicrobial effect of fungi. Considering the increasing resistance of bacteria and fungi to antibiotics and their alcoholic and aqueous extracts and essential oil on Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Candida albicans, as well as an examination cytotoxicity on gingival fibroblast cells. Alcoholic and aqueous extracts and essential oil prepared from tubers of Cyperus rotundus. After bacterial and fungal cultures, the inhibition zone, Minimum inhibitory concentrations (MIC), and Minimum Bactericidal Concentration (MBC), were studied. Cytotoxicity was also evaluated using the Methyl tetrazolium test (MTT).The results showed that Cyperus rotundus alcoholic extract had the greatest effect on inhibition of growth and death of Streptococcus mutans, Aggregatibacter actinomycetemcomitans. According to the present study, Cyperus rotundus extract can be considered as a suitable candidate for the treatment and prevention of periodontitis and tooth decay.

Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin

2021 ◽  
Author(s):  
Chiaki Tsutsumi-Arai ◽  
Yuki Arai ◽  
Chika Terada-Ito ◽  
Takahiro Imamura ◽  
Seiko Tatehara ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Denture Base ◽  
Blue Led ◽  
Led Light ◽  
Base Resin ◽  
Denture Base Resin

scholarly journals Effect of a Lactobacillus Salivarius Probiotic on a Double-Species Streptococcus Mutans and Candida Albicans Caries Biofilm

Nutrients ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 1242 ◽  
Author(s):  
Wirginia Krzyściak ◽  
Dorota Kościelniak ◽  
Monika Papież ◽  
Palina Vyhouskaya ◽  
Katarzyna Zagórska-Świeży ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Lactobacillus Salivarius

Evaluation of Antibacterial and Antifungal Properties of ZnO and Silver Nanoparticles against Candida Albicans and Streptococcus Mutans in Acrylic Resin

2021 ◽  
Author(s):  
Amin Khaleghi ◽  
Amin Archin ◽  
Mojtaba Azarian Borujeni
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Zno Nanoparticles ◽  
Acrylic Resin ◽  
Spherical Particles ◽  
Significance Level ◽  
Ultrasonic Apparatus ◽  
Experimental Laboratory ◽  
Antibacterial And Antifungal ◽  
Post Hoc

Introduction: This study aimed to evaluate the antibacterial and antifungal effect of Ag and zinc oxide nanoparticles to complete denture acrylic resin. Materials & Methods: In this experimental laboratory study, 144 samples were evaluated. The powder of acrylic resin (PMMA Ivoclar Vivadent, Lichenstein SR Triplex Hot) was mixed homogeneously in the ultrasonic apparatus with Ag (purity: 99.99%, size: 20nm, spherical) and ZnO (purity: 99%, size: 10-30 nm, nearly spherical) particles with mass fraction 0.5, 1 ,2 and 5. The microorganisms of this study were Candida albicans and Streptococcus mutans. The growth rate of microorganisms was measured by spectrophotometer based on turbidity and readings at 600 nm. Data were analyzed by ANOVA and Tukey post hoc tests. The significance level was set at 0.05. Results: Increasing Ag and ZnO nanoparticles to acrylic resin reduced the growth of Candida Albicans and Streptococcus Mutans. Even the concentration of 0.5% significantly reduced the growth of microorganisms. Complete growth inhibition was in the concentration of 5% after 24 and 48 hours. Conclusion: Increasing the Ag and ZnO nanoparticles to acrylic resin reduced the growth of microorganisms.

scholarly journals Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1645 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Positive Correlation ◽  
Unstimulated Saliva ◽  
Student’S T ◽  
Total Bacteria ◽  
Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

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