Effect of thermal treatment on the antioxidant activity of egg white hydrolysate and the preparation of novel antioxidant peptides

2021 ◽  
Author(s):  
Yue Chen ◽  
Peng Han ◽  
Bin Ma ◽  
Xuefen Wang ◽  
Meihu Ma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Thermal Treatment ◽  
Egg White ◽  
Antioxidant Peptides

scholarly journals Designing Biodegradable and Active Multilayer System by Assembling an Electrospun Polycaprolactone Mat Containing Quercetin and Nanocellulose between Polylactic Acid Films

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1288
Author(s):  
Carol López de Dicastillo ◽  
Luan Garrido ◽  
Eliezer Velásquez ◽  
Adrián Rojas ◽  
Rafael Gavara
Keyword(s):  
Antioxidant Activity ◽  
Thermal Treatment ◽  
Polylactic Acid ◽  
Active Compound ◽  
Cellulose Nanocrystals ◽  
Barrier Properties ◽  
Electrospinning Process ◽  
Multilayer Systems ◽  
Innovative Strategy ◽  
Packaged Foods

The design of multilayer systems is an innovative strategy to improve physical properties of biodegradable polymers and introduce functionality to the materials through the incorporation of an active compound into some of these layers. In this work, a trilayer film based on a sandwich of electrospun polycaprolactone (PCL) fibers (PCLé) containing quercetin (Q) and cellulose nanocrystals (CNC) between extruded polylactic acid (PLA) films was designed with the purpose of improving thermal and barrier properties and affording antioxidant activity to packaged foods. PCLé was successfully electrospun onto 70 µm-thick extruded PLA film followed by the assembling of a third 25 µm-thick commercial PLA film through hot pressing. Optical, morphological, thermal, and barrier properties were evaluated in order to study the effect of PCL layer and the addition of Q and CNC. Bilayer systems obtained after the electrospinning process of PCL onto PLA film were also evaluated. The release of quercetin from bi- and trilayer films to food simulants was also analyzed. Results evidenced that thermal treatment during thermo-compression melted PCL polymer and resulted in trilayer systems with barrier properties similar to single PLA film. Quercetin release from bi- and trilayer films followed a similar profile, but achieved highest value through the addition of CNC.

scholarly journals Encapsulation and release of egg white protein in alginate microgels: Impact of pH and thermal treatment

2019 ◽  
Vol 120 ◽  
pp. 305-311 ◽  
Author(s):  
Yujie Su ◽  
Luping Gu ◽  
Zipei Zhang ◽  
Cuihua Chang ◽  
Junhua Li ◽  
...  
Keyword(s):  
Thermal Treatment ◽  
Egg White ◽  
Egg White Protein

Naturally occurring low molecular peptides identified in egg white show antioxidant activity

2020 ◽  
Vol 138 ◽  
pp. 109766
Author(s):  
Jiexia Zheng ◽  
Tingting Bu ◽  
Ling Liu ◽  
Guoqing He ◽  
Shanshan Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Egg White ◽  
Naturally Occurring

scholarly journals Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 34 ◽  
Author(s):  
Bin Jiang ◽  
Jiaxin Na ◽  
Lele Wang ◽  
Dongmei Li ◽  
Chunhong Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase Extraction ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Two Phase ◽  
Aqueous Two Phase Extraction

At present, peptides are separated by molecular exclusion chromatography and liquid chromatography. A separation method is needed in any case, which can be scaled up for industrial scale. In this study, aqueous two-phase extraction (ATPE) and aqueous two-phase flotation (ATPF) were applied to separate and enrich antioxidant peptides from trypsin hydrolysates of whey protein isolates (WPI). The best experimental conditions were investigated, and the results were evaluated using the 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity of the peptides-per-unit concentration and the recovery rate (Y) of peptides in the top phase of both ATPE and ATPF. Under optimal conditions, the Y and ABTS free radical scavenging activity per unit concentration in top phase of ATPE could reach 38.75% and 12.94%, respectively, and in ATPF could reach 11.71% and 29.18%, respectively. The purified peptides were characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and reversed-phase high-performance liquid chromatography (RP-HPLC). PeptideCutter and PeptideMass were applied to analyze and calculate the peptide sequencing. KILDKVGINYWLAHK, VGINYWLAHKALCSEK, and TPEVDDEALEKFDKALK sequences having antioxidant activity were detected in the top phase of ATPE, and VGINYWLAHKALCSEK, KILLDKVGINYWLAHK, ILLDKVGINYWLAHK, IIAEKTKIPAVFK, KIIAEKTKIPAVFK, and VYVEELKPTPEGDLEILLQK sequences having antioxidant activity were detected in the top phase of ATPF. In conclusion, antioxidant peptides were successfully separated from the WPI hydrolysate by ATPE and ATPF; compared with ATPE, ATPF has superior specificity in separating antioxidant peptides.

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