Characterization of the Sensitizing Potential of Chemicals by In Vitro Analysis of Dendritic Cell Activation and Skin Penetration

Anti-inflammatory refers to group of medication that is utilized for curing pain and inflammation. Many synthetic products are used as anti-inflammatory agents but the effects caused by them is not satisfactory. Cordis obliqua is an anti-inflammatory herb that is enormously involved in pain reduction. The present paper aims to develop a topical gel formulation comprising herbs i.e. Cordis obliqua and curcumin with potent anti-inflammatory activity. The gel formulation was then subjected to characterization studies which involve pH, viscosity, excrudability, spreadability and in vitro analysis. pH of gel formulation was found to be 7.2±1.01 which is almost equivalent to the skin pH. In viscosity test, it was observed that viscosity of gel formulation was found to be effective. Moreover, spreadability co-efficient of the formulation satisfactory which indicates that sanitizer gel easily adhers on the skin and covers the bacteria residing on the skin, if any.

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Heat-shock protein 70-dependent dendritic cell activation by 5-aminolevulinic acid-mediated photodynamic treatment of human glioblastoma spheroids in vitro

British Journal of Cancer ◽

10.1038/bjc.2011.327 ◽

2011 ◽

Vol 105 (7) ◽

pp. 961-969 ◽

Cited By ~ 40

Author(s):

N Etminan ◽

C Peters ◽

D Lakbir ◽

E Bünemann ◽

V Börger ◽

...

Keyword(s):

Dendritic Cell ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 70 ◽

Cell Activation ◽

Aminolevulinic Acid ◽

Photodynamic Treatment ◽

Dendritic Cell Activation ◽

Human Glioblastoma

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In Vitro Analysis of the General Properties and Junctional Receptor Characteristics of Skeletal Muscle Membranes. Isolation, Purification, and Partial Characterization of Sarcolemmal Fragments

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.71.6.2435 ◽

1974 ◽

Vol 71 (6) ◽

pp. 2435-2439 ◽

Cited By ~ 16

Author(s):

B. W. Festoff ◽

W. K. Engel

Keyword(s):

Skeletal Muscle ◽

Partial Characterization ◽

General Properties ◽

Vitro Analysis ◽

In Vitro Analysis

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Anti–IL6-receptor-alpha (tocilizumab) does not inhibit human monocyte-derived dendritic cell maturation or alloreactive T-cell responses

Blood ◽

10.1182/blood-2011-06-363390 ◽

2011 ◽

Vol 118 (19) ◽

pp. 5340-5343 ◽

Cited By ~ 29

Author(s):

Brian C. Betts ◽

Erin T. St Angelo ◽

Michael Kennedy ◽

James W. Young

Keyword(s):

Cell Proliferation ◽

T Cells ◽

T Cell ◽

Dendritic Cell ◽

Cell Activation ◽

Human Monocyte ◽

T Cell Proliferation ◽

Dendritic Cell Activation ◽

Alloreactive T Cell

Abstract Significant comorbidites and lethality complicate GVHD and its treatment. Targeting the cytokine milieu may improve GVHD control; and IL6 is an attractive candidate, given its role in dendritic cell activation and T-cell differentiation. Tocilizumab is a humanized mAb to IL6-receptor-α (IL6R-α), which is Food and Drug Administration–approved for treatment of rheumatoid arthritis. Mouse transplant models have demonstrated that IL6 blockade also improves GVHD scores and survival. Definitive immunologic effects of IL6 inhibition have not emerged given inconsistent alterations in regulatory T cells (Tregs) and suppression of T-cell proliferation. Despite on-target suppression of IL6R-α signaling in human monocyte-derived dendritic cells (moDCs) and T cells, our data show no effect on moDC maturation/activation, alloreactive T-cell proliferation, Treg expansion, or allogeneic Th1/Th17 responses in vitro. These findings merit attention in any clinical trials of tocilizumab for GVHD prevention or treatment and provide a rationale for evaluating more specific inhibitors of downstream JAK2/STAT3 signaling as well.

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CXCL8 Associated Dendritic Cell Activation Marker Expression and Recruitment as Indicators of Favorable Outcomes in Colorectal Cancer

Frontiers in Immunology ◽

10.3389/fimmu.2021.667177 ◽

2021 ◽

Vol 12 ◽

Author(s):

Enhao Li ◽

Xiaobao Yang ◽

Yuzhang Du ◽

Guanzheng Wang ◽

David W. Chan ◽

...

Keyword(s):

Colorectal Cancer ◽

Dendritic Cell ◽

Cell Activation ◽

Suppressor Cells ◽

The Cancer Genome Atlas ◽

Dendritic Cell Activation ◽

Antitumor Effects ◽

Activation Markers

Accumulating evidence suggests that tumor-infiltrating immune cells (TICs) in the tumor microenvironment (TME) serve as promising therapeutic targets. CXCL8 (IL-8) may also be a potential therapeutic target in cancer. CXCL8 is a potent chemotactic factor for neutrophils, myeloid-derived suppressor cells (MDSCs) and monocytes, which are considered immunosuppressive components in cancer-bearing hosts. Here, we identified the TME-related gene CXCL8 in a high-ImmuneScore population that contributed to better survival in colorectal cancer (CRC) patients from The Cancer Genome Atlas (TCGA) database. An integrated gene profile and functional analysis of TIC proportions revealed that the dendritic cell (DC) activation markers CD80, CD83, and CD86 were positively correlated with CXCL8 expression, suggesting that CXCL8 may be functional as antitumor immune response status in the TME. The gene signature was further validated in independent GSE14333 and GSE38832 cohorts from the Gene Expression Omnibus (GEO). To test the differential contributions of immune and tumor components to progression, three CRC cell lines, CT26, MC38 and HCT116, were used. In vitro results suggested no significant growth or survival changes following treatment with an inhibitor of the CXCL8 receptor (CXCR1/2) such as reparixin or danirixin. In vivo treatment with danirixin (antagonists of CXCR2) promoted tumor progression in animal models established with CT26 cells. CXCR2 antagonism may function via an immune component, with CXCR2 antagonist treatment in mice resulting in reduced activated DCs and correlating with decreased Interferon gamma (IFN-γ) or Granzyme B expressed CD8+ T cells. Furthermore, CXCL8 induced DC migration in transwell migration assays. Taken together, our data suggested that targeting the CXCL8-CXCR2 axis might impede DC activation or recruitment, and this axis could be considered a favorable factor rather than a target for critical antitumor effects on CRC.

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