Changes with Time in the Oral Microflora and Dental Caries Induction in Hyposalivated Rats Fed on Sucrose Diet

Aim. To investigate the effect of consumption of diets with different content of fat and sugar on the state of the periodontium of rats.Methods. The rats received a semi-synthetic fat-free diet (FFD), a fat diet (5 % sunflower oil), and a sucrose diet (50 % sucrose) for 30 days. Determined the degree of periodontal atrophy, tooth decay and in the gum homogenate - the activity of elastase, catalase, urease, lysozyme and the content of malondialdehyde (MDA).Results. Sugar diet increases the degree of periodontal atrophy and tooth decay, but decreases elastase activity in the gums and increases catalase activity. Fat diet does not affect periodontal atrophy and tooth decay, but it reduces catalase activity.Conclusion. The increase in the intensity of caries and periodontal atrophy under the influence of a sucrose diet is probably due to the stimulation of the formation of lactic acid. Fat nutrition does not affect the intensity of dental caries and the degree of periodontal atrophy.

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Antimicrobial and Antibiofilm Activity of Citral and its Derivative against Microflora from Dental Plaque

Asian Journal of Organic & Medicinal Chemistry ◽

10.14233/ajomc.2020.ajomc-p259 ◽

2020 ◽

Vol 5 (2) ◽

pp. 120-126

Author(s):

S.V. Ambade ◽

N.M. Deshpande ◽

S.S. Kale ◽

V.N. Ambade

Keyword(s):

Dental Caries ◽

Essential Oil ◽

Dental Plaque ◽

Biofilm Formation ◽

Periodontal Diseases ◽

Bacterial Species ◽

Antibiofilm Activity ◽

Oral Microflora ◽

Lemon Grass ◽

First Time

Dental biofilms inhabit the oral cavity in form of dental plaque which then causes dental caries and periodontal diseases worldwide. Lemon grass essential oil (LGEO) has been reported to exhibit antimicrobial and antibiofilm activity against. This study represents the potential of citral and derivatives as antimicrobial and antibiofilm agent against dental microflora. Three bacterial species chiefly responsible for biofilm formation, and five prime colonizer of dental plaque were selected to represent dental microflora. Citral and its derivative viz. citral semicarbazone, exhibited antimicrobial and antibiofilm activity against the selected organisms. For the first time, any citral derivative has ever demonstrated to exhibit antimicrobial and antibiofilm activity against the oral microflora. However, study could not established citral or its derivatives as more effective, powerful and better herbal material as compared to LGEO to control the oral microflora associated with dental plaque.

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Identification of Microbial and Proteomic Biomarkers in Early Childhood Caries

International Journal of Dentistry ◽

10.1155/2011/196721 ◽

2011 ◽

Vol 2011 ◽

pp. 1-13 ◽

Cited By ~ 13

Author(s):

Thomas C. Hart ◽

Patricia M. Corby ◽

Milos Hauskrecht ◽

Ok Hee Ryu ◽

Richard Pelikan ◽

...

Keyword(s):

Dental Caries ◽

Predictive Models ◽

Early Childhood Caries ◽

Caries Experience ◽

Oral Flora ◽

Proteomic Data ◽

Oral Microflora ◽

Combined Data ◽

Childhood Caries

The purpose of this study was to provide a univariate and multivariate analysis of genomic microbial data and salivary mass-spectrometry proteomic profiles for dental caries outcomes. In order to determine potential useful biomarkers for dental caries, a multivariate classification analysis was employed to build predictive models capable of classifying microbial and salivary sample profiles with generalization performance. We used high-throughput methodologies including multiplexed microbial arrays and SELDI-TOF-MS profiling to characterize the oral flora and salivary proteome in 204 children aged 1–8 years (n=118caries-free,n=86caries-active). The population received little dental care and was deemed at high risk for childhood caries. Findings of the study indicate that models incorporating both microbial and proteomic data are superior to models of only microbial or salivary data alone. Comparison of results for the combined and independent data suggests that the combination of proteomic and microbial sources is beneficial for the classification accuracy and that combined data lead to improved predictive models for caries-active and caries-free patients. The best predictive model had a 6% test error, >92% sensitivity, and >95% specificity. These findings suggest that further characterization of the oral microflora and the salivary proteome associated with health and caries may provide clinically useful biomarkers to better predict future caries experience.

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The effect of differing high-carbohydrate diets on dental caries in the albino rat

British Journal Of Nutrition ◽

10.1079/bjn19660032 ◽

1966 ◽

Vol 20 (2) ◽

pp. 317-323 ◽

Cited By ~ 41

Author(s):

R. M. Green ◽

R. L. Hartles

Keyword(s):

Dental Caries ◽

Normal Growth ◽

Dietary Treatment ◽

The Other ◽

Albino Rats ◽

Maize Starch ◽

Albino Rat ◽

Group I ◽

Sucrose Diet ◽

Group 2

1. Fifty-six albino rats were distributed between four dietary groups in fourteen randomized blocks of four animals. Each block consisted of siblings and each member of the block received a different dietary treatment. 2. The diets permitted normal growth and the only variations were: group I contained 67% sucrose, group 2 an equivalent amount of uncooked maize starch and groups 3 and 467 % of finely ground Thin Wine and Morning Coffee biscuits respectively. 3. After 31 days on the diet, the animals were killed and assessed for dental caries. The rats of group I had significantly more caries than the other groups. The rats of group 2 had significantly less caries than the other groups. The biscuit diets produced significantly less caries than the sucrose diet, but significantly more caries than the starch diet.

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