The effect of Propionibacterium acidipropionici, with or without Lactobacillus plantarum, on the fermentation and aerobic stability of wheat, sorghum and maize silages

Abstract The objective of the present study was to evaluate the use of bacterial additive (Lactobacillus plantarum and Propionibacterium acidipropionici) on chemical composition, in vitro gas production, pH, losses, aerobic stability, and microbial population of corn, pearl millet, and sorghum silages in plastic bags silos (without vacuum). The experiment was carried out in a randomized block design, in a 2 × 3 factorial scheme, with or without additive ([Control] without additive and Lactobacillus plantarum [2.5 × 1010 cfu/g] and Propionibacterium acidipropionici [2.5 × 1010 cfu/g] Biomax corn, Lallemand, Saint-Simon, France [LP]) and three crops of agricultural interest; pearl millet, sorghum, and corn, with four replicates per treatment. We performed chemical analyses and in vitro gas production to determine the nutritional value of the silages. We also evaluated the aerobic stability, ammoniacal nitrogen (NH3), pH, and microbial population of the silages. The additive increased the crude protein content (P = 0.0062) in corn and sorghum and decreased the LIG content (P = 0.0567). The gas production was not affected (P > 0.05) by the additive and neither between crops. In aerobic stability, we observed that the additive affected the temperature of the sorghum silage (P = 0.0123). The additive decreased NH3 (P = 0.0095) content. The additive increased (P = 0.0441) the lactic acid bacteria population in the pearl millet, corn, and sorghum silages. Thus, the bacterial additive did not improve the fermentation profile and nutritional value of corn, pearl millet, and sorghum silages in plastic bag silos.

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Aerobic stability in corn silage (Zea maysL.) ensiled with different microbial additives

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v39i4.34426 ◽

2017 ◽

Vol 39 (4) ◽

pp. 357 ◽

Cited By ~ 1

Author(s):

Valter Harry Bumbieris Junior ◽

Vinícius André De Pietro Guimarães ◽

Ana Paula De Souza Fortaleza ◽

Fernando Luiz Massaro Junior ◽

Gabriella Jorgette de Moraes ◽

...

Keyword(s):

Zea Mays ◽

Bacillus Subtilis ◽

Experimental Design ◽

Lactobacillus Plantarum ◽

Corn Silage ◽

Control Treatment ◽

Propionibacterium Acidipropionici ◽

Aerobic Stability ◽

Corn Hybrid ◽

Plot Design

This study aimed to assess different microbial additives, regarding the efficiency of aerobic stability in corn silages. The corn hybrid used for silages production was the DKB 310. The treatments consisted of: 1) control treatment without any microbial additive; 2) Treatment with LPPA composed of: Lactobacillus plantarum CCT 0580 3.1 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 3.1 x 1010 CFU g-1; 3) Treatment with Inoculum, composed of Bacillus subtilis CCT 0089 3.0 x 109 CFU g-1, Lactobacillus plantarum CCT 0580 1.2 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 1.5 x 1010 CFU g-1 and 4) Treatment LB, composed only of Lactobacillus buchneri CCT 3746 2.6 x 1010 CFU g-1. The experimental design was completely randomized with five replicates for each treatment. For the parameters evaluated daily, we used the split plot design, in which the different silages were assigned to the plots and the time of exposure to air was assigned to the subplots. There was no difference of additives on silage pH in any of the evaluation days. The control silage reached higher temperatures indicating greater vulnerability. All additives had no aerobic instability. Silages made with the use of microbial additives were effective in maintaining the aerobic stability.

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High-moisture corn grain silage inoculated with Propionibacterium acidipropionici and Lactobacillus plantarum in different storage times

Research Society and Development ◽

10.33448/rsd-v10i1.10371 ◽

2021 ◽

Vol 10 (1) ◽

pp. e0410110371

Author(s):

Rafael Frank ◽

Marcela Abbado Neres ◽

Tatiane Fernandes ◽

Rodrigo André Schöne ◽

André Sanches de Avila ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

High Moisture ◽

Propionibacterium Acidipropionici ◽

Colony Forming Unit ◽

Ph Values ◽

Aerobic Stability ◽

Storage Times ◽

And Storage ◽

Corn Grain ◽

High Moisture Corn

The aim of this study was to evaluate the chemical composition, growth of microorganisms, and the aerobic stability of high-moisture corn grain silage inoculated with Lactobacillus plantarum + Propionibacterium acidipropionici. The experimental design was completely randomized in a 2 x 5 factorial arrangement with four replications (50 experimental units) and the treatments were: use or not of microbial inoculant (2 x 105 colony forming unit (CFU) g-1 Lactobacillus plantarum + Propionibacterium acidipropionici) and the storage length for 0, 1, 3, 7, and 14 d. Aerobic stability of silage was evaluated at 28 and 56 d of storage length. The lactic acid bacteria population was influenced by storage length and the greatest values were estimated at 8 d of storage length. Regardless of inoculant application, no enterobacteria were present from 3 d post-ensiling. With respect to mold growth, an interaction between inoculant and storage length was observed wherein molds were most abundant after 3 d of storage in silage that received inoculant. However, at 14 d of storage the use of bacterial inoculant reduced the occurrence of molds. pH values obtained after 3 d of ensiling were less than 4.0 for all the treatments. The high-moisture corn grain silage possessed good fermentative quality. Further, adequate pH values were achieved from the third day of ensilage and were not influenced by the presence of bacterial inoculant.

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Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽

10.3390/pr9050879 ◽

2021 ◽

Vol 9 (5) ◽

pp. 879

Author(s):

Seong-Shin Lee ◽

Jeong-Seok Choi ◽

Dimas Hand Vidya Paradhipta ◽

Young-Ho Joo ◽

Hyuk-Jun Lee ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Dry Matter ◽

Lactobacillus Brevis ◽

Neutral Detergent Fiber ◽

Dry Matter Digestibility ◽

Fiber Digestibility ◽

Aerobic Stability ◽

Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

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Lactobacillus plantarum and molasses alter dynamic chemical composition, microbial community and aerobic stability of mixed (amaranth and rice straw) silage

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.11171 ◽

2021 ◽

Author(s):

Lin Mu ◽

Zhan Xie ◽

Longxing Hu ◽

Guihua Chen ◽

Zhifei Zhang

Keyword(s):

Microbial Community ◽

Chemical Composition ◽

Lactobacillus Plantarum ◽

Rice Straw ◽

Aerobic Stability

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Perdas de silagens de cana-de-açúcar tratadas com aditivos químicos e bacterianos

Revista Brasileira de Zootecnia ◽

10.1590/s1516-35982007000900008 ◽

2007 ◽

Vol 36 (6 suppl) ◽

pp. 2000-2009 ◽

Cited By ~ 26

Author(s):

Gustavo Rezende Siqueira ◽

Ricardo Andrade Reis ◽

Ruben Pablo Schocken-Iturrino ◽

Aureliano José Vieira Pires ◽

Thiago Fernandes Bernardes ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Propionibacterium Acidipropionici ◽

Lactobacillus Buchneri

Objetivou-se avaliar silagens de cana-de-açúcar tratadas com aditivos químicos (uréia 1,5%; benzoato de sódio 0,1%; ou hidróxido de sódio (NaOH) 1% na matéria natural) combinados ou não com inoculantes (Propionibacterium acidipropionici + Lactobacillus plantarum ou Lactobacillus buchneri). Foram avaliadas 12 silagens: uma controle, sem inoculante e sem aditivo químico; três sem inoculante, mas com um dos aditivos; uma com P. acidipropionici + L. plantarum, sem aditivo químico; três com P. acidipropionici + L. plantarum e um dos aditivos; uma com L. buchneri, sem aditivo químico; e três com L. buchneri e um dos aditivos. Os dados foram analisados em esquema fatorial 4 × 3 com três repetições para cada tratamento. Foram determinadas as perdas ocorridas durante o processo fermentativo nas formas de gases e de efluentes e a recuperação da MS. Durante a exposição aeróbia, determinaram-se a recuperação da MS e a estabilidade aeróbia medida pela variação da temperatura. A associação de L. buchneri e NaOH reduziu as perdas por gases e efluentes e elevou a recuperação da MS. No período após abertura, destacou-se a atuação do benzoato de sódio em manter o pH com variação de apenas 0,1 unidade em cinco dias de exposição aeróbia e dos inoculantes L. buchneri e P. acidipropionici + L. plantarum em prolongar o tempo para elevação da temperatura de 34 horas nas silagens controle para 54 e 50 horas, respectivamente. A ensilagem de cana-de-açúcar requer a inclusão de algum aditivo eficiente no controle das perdas quantitativas durante a fermentação e a exposição aeróbia.

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Influência da queima e aditivos químicos e bacterianos na composição química de silagens de cana-de-açúcar

Archivos de Zootecnia ◽

10.21071/az.v58i221.5319 ◽

2007 ◽

Vol 58 (221) ◽

pp. 43-54

Author(s):

G. R. Siqueira ◽

R. A. Reis ◽

R. P. Schocken-Iturrino ◽

T. F. Bernardes ◽

A. J. V. Pires ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Propionibacterium Acidipropionici ◽

Lactobacillus Buchneri

Objetivou-se avaliar o efeito da queima e do tratamento com aditivos químicos (uréia, benzoato de sódio e hidróxido de sódio) e inoculantes microbiológicos (Propionibacterium acidipro-pionici + Lactobacillus plantarum e Lactobacillus buchneri) na ensilagem da cana-de-açúcar, utilizando o esquema fatorial 2 (cana-de-açúcar crua e queimada) x 6 (cinco aditivos uréia, benzoato de sódio, hidróxido de sódio, Propionibacterium acidipropionici + Lactobacillus plantarum e Lactobacillus buchneri mais o grupo controle). Avaliou-se a composição química da forragem antes de ensilar e após a abertura dos silos. Observou-se concentração dos teores de FDN em todas as silagens, de 51,3% (antes da ensilagem) para 67,8% após a abertura dos silos. Maiores recuperações da matéria seca digestível verdadeira, 83,6 e 79,8% foram observadas nas silagens de cana-de-açúcar queimada e tratadas com NaOH ou com L. buchneri, respectivamente. Observou-se elevação da recuperação da matéria seca digestível de 45% nas silagens de cana crua controle para 74,3% nas silagens tratadas com L. buchneri. Nas silagens de cana queimada as melhores recuperações dessa fração foram observadas nas silagens tratadas com NaOH (83,6%) e nas com L. buchneri (79,8%). Os aditivos NaOH e L. buchneri foram os mais eficientes em controlar as perdas qualitativas durante o processo fermentativo da ensilagem da cana-de-açúcar crua ou queimada.

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Effects of natamycin and Lactobacillus plantarum on the chemical composition, microbial community, and aerobic stability of Hybrid pennisetum at different temperatures

RSC Advances ◽

10.1039/d0ra00028k ◽

2020 ◽

Vol 10 (15) ◽

pp. 8692-8702 ◽

Cited By ~ 1

Author(s):

Assar Ali Shah ◽

Chen Qian ◽

Juanzi Wu ◽

Zhiwei Liu ◽

Salman Khan ◽

...

Keyword(s):

Microbial Community ◽

Chemical Composition ◽

Lactobacillus Plantarum ◽

Aerobic Stability ◽

Different Temperatures ◽

Fermentation Quality ◽

Hybrid Pennisetum

The inoculation of L. plantarum and natamycin influenced the fermentation quality. Natamycin and L. plantarum reduced the undesirable microbial community. During ensiling process, the LA and LABs was significantly enhanced.

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