Scanning immuno-electron microscopy of human leukaemia and lymphoma cells: a comparative study of techniques using immunolatex spheres as markers

1981 ◽  
Vol 123 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Haim Gamliel ◽  
Rachel Leizerowitz ◽  
Dorit Gurfel ◽  
Aaron Polliack
Keyword(s):  
Electron Microscopy ◽  
Comparative Study ◽  
Lymphoma Cells ◽  
Immuno Electron Microscopy ◽  
Human Leukaemia

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

scholarly journals Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Daniele Tognetto ◽  
Chiara De Giacinto ◽  
Alberto Armando Perrotta ◽  
Tommaso Candian ◽  
Alessandro Bova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Femtosecond Laser ◽  
Comparative Study ◽  
Surface Irregularity ◽  
Significant Difference ◽  
Group 2 ◽  
Cut Surface ◽  
Scanning Electron ◽  
Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

scholarly journals Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using aqueous extract from Anacardium occidentale leaf: comparative study of different precursors

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Eric Kwabena Droepenu ◽  
Ebenezer Aquisman Asare ◽  
Boon Siong Wee ◽  
Rafeah Binti Wahi ◽  
Frederick Ayertey ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Comparative Study ◽  
Aqueous Extract ◽  
Zinc Chloride ◽  
Zinc Acetate ◽  
Zinc Acetate Dihydrate ◽  
Inhibition Zone ◽  
Anacardium Occidentale ◽  
Acetate Dihydrate

Abstract Background Various parts of Anacardium occidentale plant possess curative qualities like antidiabetic, anti-inflammatory, antibacterial, antifungal, and antioxidant. Aqueous extract of this plant leaf was used in biosynthesizing zinc oxide (ZnO) nanoaggregates using two precursors of zinc salt (zinc acetate dihydrate [Zn(CH3COO)2∙2H2O] and zinc chloride [ZnCl2]). The synthesized ZnO samples were used in a comparative study to investigate the antibacterial activity against selected Gram-positive and Gram-negative microbes [Staphylococcus aureus, Exiguobacterium aquaticum (Gram +ve) and Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii (Gram −ve)]. The synthesized ZnO nanoaggregates from the two precursors were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive x-ray spectroscopy (EDX) techniques. Results Micrographs of SEM and TEM confirmed nanoparticles agglomerated into aggregates. While spherical nanoaggregates were identified in samples prepared from Zn(CH3COO)2∙2H2O, flake-like structures were identified in samples synthesized from ZnCl2. Particle size determined by TEM was 107.03 ± 1.54 nm and 206.58 ± 1.86 nm for zinc acetate dihydrate and zinc chloride precursors respectively. ZnO nanoaggregate synthesized using zinc acetate as precursor gave higher antibacterial activity than its counterpart, zinc chloride with K. pneumonia recording the highest inhibition zone of 2.08 ± 0.03 mm (67.53%) whereas S. aureus recorded the least inhibition zone of 1.06 ± 0.14 mm (34.75%) for ZnO nanoaggregate from zinc chloride precursor. Also, antibacterial activity increases with increasing concentration of the extract in general. However, A. baumannii, E. aquaticum, and K. pneumoniae did not follow the continuity trend with regards to the 250 ppm and 500 ppm concentrations. Conclusion Biosynthesis of ZnO nanoaggregates using aqueous extract of A. occidentale leaf from zinc acetate dihydrate and zinc chloride as precursors was successful with the formation of nanospheres and nanoflakes. The study suggested that A. occidentale sp. could be an alternative source for the production of ZnO nanoparticles and are efficient antibacterial compounds against both Gram +ve and Gram −ve microbes with its promising effect against infectious bacteria.

scholarly journals Morphological Alterations and Increased S100B Expression in Epidermal Langerhans Cells Detected in Skin from Patients with Progressive Vitiligo

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 579
Author(s):  
Fei Yang ◽  
Lingli Yang ◽  
Lanting Teng ◽  
Huimin Zhang ◽  
Ichiro Katayama
Keyword(s):  
Electron Microscopy ◽  
Langerhans Cells ◽  
Critical Role ◽  
Light And Electron Microscopy ◽  
Morphological Alterations ◽  
Birbeck Granules ◽  
Immuno Electron Microscopy ◽  
Skin Biopsies ◽  
Epidermal Langerhans Cells

The role of Langerhans cells (LCs) in vitiligo pathogenesis remains unclear, with published studies reporting contradictory results regarding the quantity of LCs and no data on the features of LCs in vitiligo. Here, we aimed to analyze the presence, density, and morphological features of LCs in the epidermis of patients with vitiligo. Skin biopsies were stained for LCs using anti-CD1a/anti-langerin antibodies and analyzed by immunocytochemistry with light and electron microscopy. Compared with healthy controls, we detected significantly increased numbers of epidermal LCs in lesional skin from vitiligo in the progressive state. These LCs exhibited striking morphological alterations, including an elevated number of dendrites, with increased length and more branches than dendrites from controls. Ultrastructure examination via immuno-electron microscopy revealed markedly reduced Birbeck granules (BGs) and shorter BG rods in LCs from progressive vitiligo, with higher expression of langerin. Additionally, expression of S100B, the activity biomarker of vitiligo, was increased in these LCs. This work provides new insight on the cellular composition of LCs in vitiliginous skin, revealing altered morphology and increased LC numbers, with elevated S100B expression. Our data suggest LCs might play a critical role in vitiligo pathogenesis and thus may represent a novel therapeutic target for this disease.

Immuno-electron Microscopy of Hepatitis B Antigen in Liver

1972 ◽  
Vol 140 (4) ◽  
pp. 1334-1339 ◽  
Author(s):  
M. A. Gerber ◽  
F. Schaffner ◽  
F. Paronetto
Keyword(s):  
Electron Microscopy ◽  
Hepatitis B ◽  
Immuno Electron Microscopy ◽  
Hepatitis B Antigen

scholarly journals Functional Entry of Baculovirus into Insect and Mammalian Cells Is Dependent on Clathrin-Mediated Endocytosis

2006 ◽  
Vol 80 (17) ◽  
pp. 8830-8833 ◽  
Author(s):  
Gang Long ◽  
Xiaoyu Pan ◽  
Richard Kormelink ◽  
Just M. Vlak
Keyword(s):  
Electron Microscopy ◽  
Mammalian Cells ◽  
Low Ph ◽  
Coated Pits ◽  
Immuno Electron Microscopy ◽  
Endocytosis Pathway ◽  
Ph Dependent ◽  
Budded Virus

ABSTRACT Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits. This insight is primarily based on (immuno)electron microscopy studies but requires biochemical support to exclude the use of other pathways. Here, we demonstrate using various inhibitors that functional entry of baculoviruses into insect and mammalian cells is primarily dependent on clathrin-mediated endocytosis. Our results further suggest that caveolae are somehow involved in baculovirus entry in mammalian cells. A caveolar endocytosis inhibitor, genistein, enhances baculovirus transduction in these cells considerably.

A Comparative Study of Trichomes in Angophora Cav. And Eucalyptus L'hérit. - A Question of Homology

1984 ◽  
Vol 32 (5) ◽  
pp. 561 ◽  
Author(s):  
PY Ladiges
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Study ◽  
Light Microscopy ◽  
Evolutionary Trends ◽  
Oil Glands ◽  
Ancestral Condition ◽  
Scanning Electron

The trichomes of Angophora and Eucalyptus are illustrated from scanning electron microscopy and light microscopy, and evolutionary trends are discussed. Bristle glands of Angophora and Eucalyptus subgen. Blakella and Corymbia are emergent oil glands of varying lengths. Emergent oil glands occur in all other Eucalyptus subgenera but they are most conspicuous in Blakella, Corymbia and Angophora, in which they are characterized by four cap cells each ornamented with micropapillae. Hairs in Angophora are unique, being multicellular; they are also uniseriate and scattered on the epidermis. In contrast, hairs in Eucalyptus are simple extensions, short or long, of the cells on the sides of or the cap cells of the emergent oil glands, and they are not homologous with those of Angophora. Eucalyptus setosa (subgen. Blakella) and E. brockwayi (subgen. Symphyomyrtus) are two exceptions, having unicellular hairs on the epidermis, not associated with oil glands. It is suggested that this is an ancestral condition (or secondary reversal to it).

Sign in / Sign up

Export Citation Format

Share Document