COMPARATIVE STUDY OF THE INTRACYTOPLASMIC INCLUSIONS IN LAFORA DISEASE AND TYPE IV GLYCOGENOSIS BY ELECTRON MICROSCOPY

Glycogenosis is defined as any condition in which the tissue concentration of glycogen is increased. There are currently ten recognized variants of glycogenosis that are heritable inborn errors of metabolism. The specific enzymatic defect in each of the variants is known or at least suspected. In all cases, the enzymatic defect prevents the proper metabolism or formation of the glycogen molecule. The clinical and histologic differences between the types of glycogenosis is important to a proper diagnosis after the presence of such a condition is realized. This study was initiated to examine the ultrastructure of the rare Type IV Glycogenosis (Amylopectinosis) of which there is very little morphologic characterization in the literature.Liver tissue was obtained by needle biopsy from a 12-month-old Oriental female who was originally admitted to the hospital after observation of poor development, loss of appetite, and hepatomegaly. The majority of the tissue was fixed for light microscopy in neutral buffered formalin and processed using routine and special staining procedures (reticulin, trichrome, iron, copper, PAS, PAS-diastase and PAS-pectinase.

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Faculty Opinions recommendation of Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.737913713.793574422 ◽

2020 ◽

Author(s):

Edward Egelman

Keyword(s):

Electron Microscopy ◽

Distinct Type ◽

Type Iv Pili ◽

Type Iv ◽

Cryo Electron Microscopy

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Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

Journal of Ophthalmology ◽

10.1155/2018/8620150 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Daniele Tognetto ◽

Chiara De Giacinto ◽

Alberto Armando Perrotta ◽

Tommaso Candian ◽

Alessandro Bova ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Femtosecond Laser ◽

Comparative Study ◽

Surface Irregularity ◽

Significant Difference ◽

Group 2 ◽

Cut Surface ◽

Scanning Electron ◽

Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

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Early stages of high-temperature oxidation of Ni- and Co-base model superalloys: A comparative study using rapid thermal annealing and advanced electron microscopy

Corrosion Science ◽

10.1016/j.corsci.2021.109744 ◽

2021 ◽

pp. 109744

Author(s):

Dorota Kubacka ◽

Martin Weiser ◽

Erdmann Spiecker

Keyword(s):

Electron Microscopy ◽

High Temperature ◽

Comparative Study ◽

Thermal Annealing ◽

Rapid Thermal Annealing ◽

High Temperature Oxidation ◽

Temperature Oxidation ◽

Early Stages

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Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using aqueous extract from Anacardium occidentale leaf: comparative study of different precursors

Beni-Suef University Journal of Basic and Applied Sciences ◽

10.1186/s43088-020-00091-7 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Eric Kwabena Droepenu ◽

Ebenezer Aquisman Asare ◽

Boon Siong Wee ◽

Rafeah Binti Wahi ◽

Frederick Ayertey ◽

...

Keyword(s):

Electron Microscopy ◽

Antibacterial Activity ◽

Comparative Study ◽

Aqueous Extract ◽

Zinc Chloride ◽

Zinc Acetate ◽

Zinc Acetate Dihydrate ◽

Inhibition Zone ◽

Anacardium Occidentale ◽

Acetate Dihydrate

Abstract Background Various parts of Anacardium occidentale plant possess curative qualities like antidiabetic, anti-inflammatory, antibacterial, antifungal, and antioxidant. Aqueous extract of this plant leaf was used in biosynthesizing zinc oxide (ZnO) nanoaggregates using two precursors of zinc salt (zinc acetate dihydrate [Zn(CH3COO)2∙2H2O] and zinc chloride [ZnCl2]). The synthesized ZnO samples were used in a comparative study to investigate the antibacterial activity against selected Gram-positive and Gram-negative microbes [Staphylococcus aureus, Exiguobacterium aquaticum (Gram +ve) and Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii (Gram −ve)]. The synthesized ZnO nanoaggregates from the two precursors were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive x-ray spectroscopy (EDX) techniques. Results Micrographs of SEM and TEM confirmed nanoparticles agglomerated into aggregates. While spherical nanoaggregates were identified in samples prepared from Zn(CH3COO)2∙2H2O, flake-like structures were identified in samples synthesized from ZnCl2. Particle size determined by TEM was 107.03 ± 1.54 nm and 206.58 ± 1.86 nm for zinc acetate dihydrate and zinc chloride precursors respectively. ZnO nanoaggregate synthesized using zinc acetate as precursor gave higher antibacterial activity than its counterpart, zinc chloride with K. pneumonia recording the highest inhibition zone of 2.08 ± 0.03 mm (67.53%) whereas S. aureus recorded the least inhibition zone of 1.06 ± 0.14 mm (34.75%) for ZnO nanoaggregate from zinc chloride precursor. Also, antibacterial activity increases with increasing concentration of the extract in general. However, A. baumannii, E. aquaticum, and K. pneumoniae did not follow the continuity trend with regards to the 250 ppm and 500 ppm concentrations. Conclusion Biosynthesis of ZnO nanoaggregates using aqueous extract of A. occidentale leaf from zinc acetate dihydrate and zinc chloride as precursors was successful with the formation of nanospheres and nanoflakes. The study suggested that A. occidentale sp. could be an alternative source for the production of ZnO nanoparticles and are efficient antibacterial compounds against both Gram +ve and Gram −ve microbes with its promising effect against infectious bacteria.

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A Comparative Study of Trichomes in Angophora Cav. And Eucalyptus L'hérit. - A Question of Homology

Australian Journal of Botany ◽

10.1071/bt9840561 ◽

1984 ◽

Vol 32 (5) ◽

pp. 561 ◽

Cited By ~ 14

Author(s):

PY Ladiges

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Comparative Study ◽

Light Microscopy ◽

Evolutionary Trends ◽

Oil Glands ◽

Ancestral Condition ◽

Scanning Electron

The trichomes of Angophora and Eucalyptus are illustrated from scanning electron microscopy and light microscopy, and evolutionary trends are discussed. Bristle glands of Angophora and Eucalyptus subgen. Blakella and Corymbia are emergent oil glands of varying lengths. Emergent oil glands occur in all other Eucalyptus subgenera but they are most conspicuous in Blakella, Corymbia and Angophora, in which they are characterized by four cap cells each ornamented with micropapillae. Hairs in Angophora are unique, being multicellular; they are also uniseriate and scattered on the epidermis. In contrast, hairs in Eucalyptus are simple extensions, short or long, of the cells on the sides of or the cap cells of the emergent oil glands, and they are not homologous with those of Angophora. Eucalyptus setosa (subgen. Blakella) and E. brockwayi (subgen. Symphyomyrtus) are two exceptions, having unicellular hairs on the epidermis, not associated with oil glands. It is suggested that this is an ancestral condition (or secondary reversal to it).

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A Comparative Study of Impact Effect of Composite Cylinders and Type IV Pressure Vessels

Emerging Materials Research ◽

10.1680/jemmr.20.00029 ◽

2021 ◽

Vol 10 (1) ◽

pp. 1-12

Author(s):

Naseer H Farhood ◽

Saravanan Karuppanan ◽

Hamdan H Ya ◽

Wisam A Abdul-Lateef

Keyword(s):

Comparative Study ◽

Pressure Vessels ◽

Impact Effect ◽

Type Iv ◽

Composite Cylinders

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Purification and Three-Dimensional Electron Microscopy Structure of the Neisseria meningitidis Type IV Pilus Biogenesis Protein PilG

Journal of Bacteriology ◽

10.1128/jb.00648-07 ◽

2007 ◽

Vol 189 (17) ◽

pp. 6389-6396 ◽

Cited By ~ 25

Author(s):

Richard F. Collins ◽

Muhammad Saleem ◽

Jeremy P. Derrick

Keyword(s):

Electron Microscopy ◽

Neisseria Meningitidis ◽

Metal Ion ◽

Polyacrylamide Gel Electrophoresis ◽

Three Dimensional ◽

Type Ii Secretion ◽

Type Ii ◽

Type Iv Pilus ◽

Type Iv ◽

Pilus Biogenesis

ABSTRACT Type IV pili are surface-exposed retractable fibers which play a key role in the pathogenesis of Neisseria meningitidis and other gram-negative pathogens. PilG is an integral inner membrane protein and a component of the type IV pilus biogenesis system. It is related by sequence to the extensive GspF family of secretory proteins, which are involved in type II secretion processes. PilG was overexpressed and purified from Escherichia coli membranes by detergent extraction and metal ion affinity chromatography. Analysis of the purified protein by perfluoro-octanoic acid polyacrylamide gel electrophoresis showed that PilG formed dimers and tetramers. A three-dimensional (3-D) electron microscopy structure of the PilG multimer was determined using single-particle averaging applied to samples visualized by negative staining. Symmetry analysis of the unsymmetrized 3-D volume provided further evidence that the PilG multimer is a tetramer. The reconstruction also revealed an asymmetric bilobed structure approximately 125 Å in length and 80 Å in width. The larger lobe within the structure was identified as the N terminus by location of Ni-nitrilotriacetic acid nanogold particles to the N-terminal polyhistidine tag. We propose that the smaller lobe corresponds to the periplasmic domain of the protein, with the narrower “waist” region being the transmembrane section. This constitutes the first report of a 3-D structure of a member of the GspF family and suggests a physical basis for the role of the protein in linking cytoplasmic and periplasmic protein components of the type II secretion and type IV pilus biogenesis systems.

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