scholarly journals Ecophysiology of associative nitrogen fixation in a rhizosphere model in pure and mixed culture

1991 ◽  
Vol 85 (4) ◽  
pp. 279-292 ◽  
Author(s):  
C. Fritzsche ◽  
K. Huckfeldt ◽  
E.-G. Niemann
1939 ◽  
Vol 29 (2) ◽  
pp. 302-305 ◽  
Author(s):  
E. H. Richards

1. A study was made of nitrogen-fixation byAzotobacter chroococcumalone in a medium containing dextrose (which it can utilize) and in mixture with a coliform organism on a medium containing no carbohydrate except starch, whichAzotobactercannot utilize unless it be hydrolysed by the coliform organism or some other agency.2. The amount of nitrogen fixed in the mixed cultures was found to be maximal at two temperatures, and a discussion is given of the causes thought to be operative in producing the double maximum.


1988 ◽  
Vol 110 (2) ◽  
pp. 321-329 ◽  
Author(s):  
R. Rai

SummaryHigh-temperature-adapted strains RAU 1, RAU 2 and RAU 3 ofAzospirillum brasilenseC 7 were isolated from stepwise transfer to higher temperature (30 to 42 °C). One of the strains (RAU 1) showed more growth, greater nitrogenase and hydrogenase activities at 30 and 42 °C than parental and other temperature-adapted strains. This strain also showed growth and more nitrogenase activity from pH 6·5 to 8·0. Strain RAU 1 showed cross-resistance to penicillin (300/µg/ml) but not to streptomycin, kanamycin, viomycin and polymixin B at 30 and 42 °C. It was demonstrated in field plots in calcareous soil that seed inoculation with RAU 1 enhanced mineral uptake of cheena. Inoculation with RAU 1 led to a significant increase in associative nitrogen fixation, dry weight of roots, grain and straw yield of cheena compared with the uninoculated control with or without applied N, but the effect of seed inoculation with high-temperature-adapted strains was variable with different genotypes of cheena.


The fixation of nitrogen by Azotobacter and Pseudomonas depends upon the presence of a fermentable carbohydrate as a source of energy. Although a large number of carbohydrates can be utilised by these organisms there is a considerable variation in their efficiency, and for effective fixation it is necessary to work with a sugar specially suitable for the organism under investigation. It is generally recognised that mannite is the most efficient kind for Azotobacter and maltose for Pseudomonas . Unfortunately mannite is practically useless for Pseudomonas and maltose for Azotobacter . Further it was found when working with a mixed culture of Azotobacter and Pseudomonas that the mixture of carbohydrates (mannite and maltose) was not satisfactory. It was desirable, therefore, to obtain a carbohydrate which would be equally efficient as a source of energy for both organisms. This has been obtained in dextrin. In order to compare the influence of dextrin with mannite and maltose upon Azotobacter and Pseudomonas respectively, six series of Erlenmeyer flasks, four flasks in each series, were arranged. All the flasks contained 100 c. c. of a nutrient solution consisting of di-potassium phosphate 0·2 grm., magnesium sulphate 0·02 grm., basic slag 0·4 grm., in 100 c. c. distilled water.


2008 ◽  
Vol 7 ◽  
pp. 57-68
Author(s):  
V.V. Volkogon ◽  
O.M. Berdnikov ◽  
E.I. Volkogon ◽  
N.P. Shtan’ko

The paper covers research data on technology elaboration of new biological preparation Microhumin. It was shown that combination of biologically active biohumus extract with the suspension of active nitrogen fixing strain of azospirills in certain ratio results in creation of preparation performing additive stimulatory effect on growth and development of spring barley plants and activity of associative nitrogen fixation.


1994 ◽  
Vol 47 (3) ◽  
pp. 192 ◽  
Author(s):  
John J. Brejda ◽  
Robert J. Kremer ◽  
James R. Brown

2008 ◽  
Vol 6 ◽  
pp. 29-38
Author(s):  
V.V. Volkogon ◽  
O.I. Bakun ◽  
E.I. Volkogon ◽  
N.P. Shtanko ◽  
P.G. Dulnev

The influence of plant growth regulator triman-1 on nitrogen fixing bacteria and nitrogen fixation process in barley root zone was studied in the laboratory and field conditions. It was shown that triman- 1 enhances associative nitrogen fixation activity when mineral nitrogen fertilizers (N30) was used. The use of triman-1 increases efficiency of associative symbiosis more effectively with the use of carboammonium salts rather than with ammonium nitrate.


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