The fixation of nitrogen by
Azotobacter
and
Pseudomonas
depends upon the presence of a fermentable carbohydrate as a source of energy. Although a large number of carbohydrates can be utilised by these organisms there is a considerable variation in their efficiency, and for effective fixation it is necessary to work with a sugar specially suitable for the organism under investigation. It is generally recognised that mannite is the most efficient kind for
Azotobacter
and maltose for
Pseudomonas
. Unfortunately mannite is practically useless for
Pseudomonas
and maltose for
Azotobacter
. Further it was found when working with a mixed culture of
Azotobacter
and
Pseudomonas
that the mixture of carbohydrates (mannite and maltose) was not satisfactory. It was desirable, therefore, to obtain a carbohydrate which would be equally efficient as a source of energy for both organisms. This has been obtained in dextrin. In order to compare the influence of dextrin with mannite and maltose upon
Azotobacter
and
Pseudomonas
respectively, six series of Erlenmeyer flasks, four flasks in each series, were arranged. All the flasks contained 100 c. c. of a nutrient solution consisting of di-potassium phosphate 0·2 grm., magnesium sulphate 0·02 grm., basic slag 0·4 grm., in 100 c. c. distilled water.