scholarly journals The prpZ gene cluster encoding eukaryotic-type Ser/Thr protein kinases and phosphatases is repressed by oxidative stress and involved in Salmonella enterica serovar Typhi survival in human macrophages

2008 ◽  
Vol 281 (2) ◽  
pp. 160-166 ◽  
Author(s):  
Sébastien P. Faucher ◽  
Charles Viau ◽  
Pierre-Paul Gros ◽  
France Daigle ◽  
Hervé Le Moual
Keyword(s):  
Oxidative Stress ◽  
Gene Cluster ◽  
Protein Kinases ◽  
Salmonella Enterica ◽  
Human Macrophages ◽  
Salmonella Enterica Serovar Typhi

Protection against experimental salmonellosis by recombinant 49 kDa OMP of Salmonella enterica serovar Typhi: biochemical aspects

Biologia ◽  
2013 ◽  
Vol 68 (2) ◽  
Author(s):  
Jasmine Kaur ◽  
Swatantra Jain
Keyword(s):  
Oxidative Stress ◽  
Salmonella Enterica ◽  
Protective Immunity ◽  
Outer Membrane Proteins ◽  
Lethal Dose ◽  
Multidrug Resistant ◽  
Strong Immune Response ◽  
Salmonella Enterica Serovar Typhi ◽  
Biochemical Estimation ◽  
Reported Data

AbstractTyphoid fever is systemic illness caused by Salmonella enterica serovar Typhi (S. Typhi) in humans. Increasing multidrug resistant strains of S. Typhi and limited effect of available vaccines has necessitated exploring of new immunogens for protection against it. Earlier studies have shown that a crude preparation of outer membrane proteins (OMPs) of S. Typhi evokes strong immune response and induces a protective immunity against infection caused by diverse Gram-negative bacteria. In the present study we have evaluated the protective effect of a purified recombinant 49 kDa (r49kDa) OMP of S. Typhi alone or along with alum or complete Freund’s adjuvant, against a challenge by S. Typhi (0.4 × 50% lethal dose) by biochemical estimation of serum enzymes and oxidative stress enzymes in Swiss albino mice. There was a decrease in activity of alanine aminotransferase by 14.28%, 38.09%, 23.80%; aspartate aminotransferase by 6.25%, 25%, 16.25%; lipid peroxidation by 4.34%, 18.84%, 11.59%; and catalase by 8%, 14%, 10%, respectively, whereas increase in activity of reduced glutathione by 33.33%, 61.11%, 44.44%; glutathione peroxidase by 7%, 16%, 10%; and glutathione reductase by 8%, 20%, 12%, respectively, as compared to control animals challenged with bacteria without pre-immunization. The results indicated that immunization of mice with r49kDa OMP alone or in combination with adjuvants protected and normalized the liver. It reduces the development of oxidative stress in mice against Salmonella infection and the risk of getting typhoid. These results represent an additional supplement to our earlier reported data on protective immunity evoked by this protein.

scholarly journals Salmonella enterica Serovar Typhi Induces Host Metabolic Reprogramming to Increase Glucose Availability for Intracellular Replication

2021 ◽  
Vol 22 (18) ◽  
pp. 10003
Author(s):  
Jingting Wang ◽  
Shuai Ma ◽  
Wanwu Li ◽  
Xinyue Wang ◽  
Di Huang ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Salmonella Enterica ◽  
Metabolic Reprogramming ◽  
Host Cells ◽  
Uptake System ◽  
Intracellular Replication ◽  
Glucose Content ◽  
Human Macrophages ◽  
Salmonella Enterica Serovar Typhi ◽  
The Impact

Salmonella enterica serovar Typhi (S. Typhi) is a human-limited intracellular pathogen and the cause of typhoid fever, a severe systemic disease. Pathogen–host interaction at the metabolic level affects the pathogenicity of intracellular pathogens, but it remains unclear how S. Typhi infection influences host metabolism for its own benefit. Herein, using metabolomics and transcriptomics analyses, combined with in vitro and in vivo infection assays, we investigated metabolic responses in human macrophages during S. Typhi infection, and the impact of these responses on S. Typhi intracellular replication and systemic pathogenicity. We observed increased glucose content, higher rates of glucose uptake and glycolysis, and decreased oxidative phosphorylation in S. Typhi-infected human primary macrophages. Replication in human macrophages and the bacterial burden in systemic organs of humanized mice were reduced by either the inhibition of host glucose uptake or a mutation of the bacterial glucose uptake system, indicating that S. Typhi utilizes host-derived glucose to enhance intracellular replication and virulence. Thus, S. Typhi promotes its pathogenicity by inducing metabolic changes in host macrophages and utilizing the glucose that subsequently accumulates as a nutrient for intracellular replication. Our findings provide the first metabolic signature of S. Typhi-infected host cells and identifies a new strategy utilized by S. Typhi for intracellular replication.

scholarly journals Selective Capture of Salmonella enterica Serovar Typhi Genes Expressed in Macrophages That Are Absent from the Salmonella enterica Serovar Typhimurium Genome

2005 ◽  
Vol 73 (8) ◽  
pp. 5217-5221 ◽  
Author(s):  
Sébastien P. Faucher ◽  
Roy Curtiss ◽  
France Daigle
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Pathogenicity Islands ◽  
Human Macrophages ◽  
Salmonella Enterica Serovar Typhi ◽  
Serovar Typhimurium ◽  
Transcribed Sequences

ABSTRACT Thirty-six Salmonella enterica serovar Typhi-specific genes, absent from the Salmonella enterica serovar Typhimurium genome, that were expressed in human macrophages were identified by selective capture of transcribed sequences. These genes are located on 15 unique loci of the serovar Typhi genome, including Salmonella pathogenicity islands (SPI-7, SPI-8, and SPI-10) and bacteriophages (ST15, ST18, and ST35).

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