Identification and application of RAPD markers for anthracnose resistance in water yam (Dioscorea alata)

Anthracnose, caused by Colletotrichum gloeosporioides, is one of the major constraints limiting water yam (Dioscorea alata) production in the tropics. In this region, yam anthracnose control is mostly achieved by the deployment of moderately resistant yam genotypes. Therefore, screening for new sources of anthracnose resistance is an important aspect of yam research in the tropics. The reliability and applicability of different yam anthracnose rating parameters has not been fully examined. Disease severity on detached leaves in the laboratory and leaf severity, lesion size, and spore production on whole plants in the greenhouse were used to screen an F1 yam population and correlate screening results with field evaluations. Anthracnose lesion size had the smallest predicted residual means but whole-plant severity and detached-leaf severity had the best variance homogeneity and relatively small predicted residual means. The concordance correlation coefficient (rc) and κ statistic were used to determine the agreement between anthracnose rating parameters and field evaluations. Detached-leaf (rc = 0.95, κ = 0.81) and whole-plant (rc = 0.96, κ = 0.86) evaluations had high positive agreement with field evaluation but spore production (κ = 0.69) and lesion size (κ = 0.57) had moderate positive agreement. These results suggest that all the evaluated rating parameters can be used to successfully screen yam germplasm for anthracnose resistance but lesion size and spore production data may need to be transformed.

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A SNP-Based Linkage Map and QTL Identification for Resistance to Yam Anthracnose Disease (YAD) in Water Yam (Dioscorea alata)

10.21203/rs.3.rs-26806/v1 ◽

2020 ◽

Author(s):

Kwabena Darkwa ◽

Paterne AGRE ◽

Bunmi Olasanmi ◽

Olufisayo Kolade ◽

Pierre Mournet ◽

...

Keyword(s):

Disease Resistance ◽

Linkage Map ◽

High Density ◽

Genetic Maps ◽

Dioscorea Alata ◽

Valuable Insight ◽

Phenotypic Variance ◽

Anthracnose Resistance ◽

Anthracnose Disease ◽

Water Yam

Abstract Background: Yam anthracnose disease (YAD) caused by Colletotrichum gloeosporioides is the primary cause of yield loss in water yam (Dioscorea alata), the widely cultivated species of yam. Development of resistant cultivars have been a prime target for sustainable management of anthracnose in water yam. Molecular breeding tools are required to expedite the development of improved yam varieties. QTL analysis using high density genetic maps serve as a powerful tool to discover key locations of quantitave traits. This study aimed at tagging quantitative trait loci (QTL) for anthracnose disease resistance in a bi-parental mapping population of D. alata.Results: In this study, two contrasting parents for yam anthracnose disease reaction and their 204 full- sib offspring were used to develop a high-density genetic linkage map with 3,257 SNP markers by the GBS technique. The total length of the consensus map was 1460.94 cM with an average of 163 markers per chromosome. Four QTLs were detected for anthracnose disease resistance in 4 locations on 3 chromosomes. The proportion of phenotypic variance explained by these QTLs ranged from 10 to 13%. Plant defense response genes including GDSL-like Lipase/Acylhydrolase, Protein kinase domain and F-box protein were also detected within the QTL regions. Conclusion: The results from the present study provide valuable insight into the genetic architecture of anthracnose resistance in water yam. The candidate markers and putative genes identified herewith form a relevant resource to apply marker-assisted selection as alternative to a conventional labor-intensive screening for anthracnose resistance in water yam.

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A genetic linkage map of water yam (Dioscorea alata L.) based on AFLP markers and QTL analysis for anthracnose resistance

Theoretical and Applied Genetics ◽

10.1007/s00122-002-0912-6 ◽

2002 ◽

Vol 105 (5) ◽

pp. 726-735 ◽

Cited By ~ 41

Author(s):

H. Mignouna ◽

R. Mank ◽

T. Ellis ◽

N. van den Bosch ◽

R. Asiedu ◽

...

Keyword(s):

Linkage Map ◽

Qtl Analysis ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Aflp Markers ◽

Dioscorea Alata ◽

Anthracnose Resistance ◽

Water Yam

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Anatomical observation on release of tuber dormancy in water yam (Dioscorea alata L.)

African Journal of Agricultural Research ◽

10.5897/ajar12.1774 ◽

2013 ◽

Vol 8 (16) ◽

pp. 1365-1369

Author(s):

Shiwachi Hironobu ◽

Chen Shuwan ◽

Matsumoto Ryo ◽

Sanada Atusi ◽

Toyohara Hidekazu

Keyword(s):

Dioscorea Alata ◽

Tuber Dormancy ◽

Water Yam

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UTILIZATION OF WATER YAM (Dioscorea alata) AS A SOURCE OF ETHANOL

Journal of Chemical Society of Nigeria ◽

10.46602/jcsn.v46i1.577 ◽

2021 ◽

Vol 46 (1) ◽

Author(s):

A.M. Hashimi

Keyword(s):

Raw Materials ◽

Primary Alcohol ◽

Raw Material ◽

Dioscorea Alata ◽

Fermentable Sugar ◽

Iodine Solution ◽

Indigenous Production ◽

Job Opportunity ◽

Alternative Sources ◽

Water Yam

Ethanol (C2H5OH) is a primary alcohol which can be produced by fermentation, using any of the sources of carbohydrates such as sugarcane, cassava, grains and yam tubers among others. Production of ethanol fermented from renewable sources for fuel and fuel additives are known as bioethanol. Since the need of bioethanol is increasing, the production of bioethanol must be increased using cheaper and eco friendly raw materials. This study was conducted, using cheaper and eco friendly raw materials; water yam (Dioscorea alata) as alternative sources for ethanol production. The starch that was produced from water yam was hydrolysed into fermentable sugar, using acid hydrolysis. The fermentable sugar was converted into ethanol by fermentation process at pH 4.5 using fermenting brewer's yeast (Saccharomyces calsbergensis). Starch, glucose and ethanol which were obtained were analysed for physicochemical properties. Findings revealed that the starch sample obtained gave a blue-black colouration on addition of few drops of iodine solution. This confirmed that the carbohydrate content was mainly starch. On calculation of viability, findings further showed that water yam is a good raw material for production of ethanol, therefore indigenous production of ethanol obtained from water yam is economically viable and recommended. This will save the country from lot of money spends annually on the importation of ethanol and it can also provide job opportunity for many wandering and unemployed Nigerians.

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The sprouting process in yam (Dioscorea spp.) tuber pieces

The Journal of Agricultural Science ◽

10.1017/s0021859600086408 ◽

1973 ◽

Vol 81 (3) ◽

pp. 375-379 ◽

Cited By ~ 17

Author(s):

I. C. Onwueme

Keyword(s):

Dioscorea Alata ◽

Shoot Buds ◽

Shoot Bud ◽

Meristematic Cells ◽

Cell Divisions ◽

Large Tuber ◽

Extensive Cell ◽

Water Yam

SUMMARYThe progression of sprouting was observed in water-yam (Dioscorea alata) and whiteyam (D. rotundata) tuber pieces planted in moist sawdust. Soon after planting, white callus-like protuberances (sprouting loci) were seen on the tuber surface, followed by the appearance of one or more differentiated shoot buds on the sprouting locus. Sprouting loci appeared more readily on the upper and lower parts of each tuber piece than on the sides. Large tuber pieces sprouted more rapidly, had larger and more numerous sprouting loci, and gave rise to more shoot buds than small tuber pieces.Anatomical examination showed that the shoot bud resulted from the activity of a layer of meristematic cells lying close to the tuber surface. Extensive cell divisions commenced in this layer after planting, and some of the resulting cells soon differentiated into the shoot bud.

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Quantification of the aggressiveness of a foliar pathogen, Colletotrichum gloeosporioides, responsible for water yam (Dioscorea alata) anthracnose

European Journal of Plant Pathology ◽

10.1007/s10658-012-9986-4 ◽

2012 ◽

Vol 134 (2) ◽

pp. 267-279 ◽

Cited By ~ 3

Author(s):

Lise Frézal ◽

Loic Desquilbet ◽

Guy Jacqua ◽

Claire Neema

Keyword(s):

Colletotrichum Gloeosporioides ◽

Dioscorea Alata ◽

Foliar Pathogen ◽

Water Yam

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The Partial Characterization of a Badnavirus Infecting the Greater Asiatic or Water Yam (Dioscorea alata)

Journal of Phytopathology ◽

10.1111/j.1439-0434.1999.tb03829.x ◽

1999 ◽

Vol 147 (5) ◽

pp. 265-269 ◽

Cited By ~ 23

Author(s):

S. Phillips ◽

R. W. Briddon ◽

A. A. Brunt ◽

R. Hull

Keyword(s):

Partial Characterization ◽

Dioscorea Alata ◽

Water Yam

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Genome-Wide Association Analysis for Tuber Dry Matter and Oxidative Browning in Water Yam (Dioscorea alata L.)

Plants ◽

10.3390/plants9080969 ◽

2020 ◽

Vol 9 (8) ◽

pp. 969 ◽

Cited By ~ 1

Author(s):

Cobes Gatarira ◽

Paterne Agre ◽

Ryo Matsumoto ◽

Alex Edemodu ◽

Victor Adetimirin ◽

...

Keyword(s):

Association Analysis ◽

Dry Matter ◽

Significant Snps ◽

Genome Wide Association ◽

Enzymatic Oxidation ◽

Dioscorea Alata ◽

Quality Traits ◽

Genome Wide ◽

Tuber Dry Matter ◽

Water Yam

Yam (Dioscorea spp.) is a nutritional and medicinal staple tuber crop grown in the tropics and sub-tropics. Among the food yam species, water yam (Dioscorea alata L.) is the most widely distributed and cultivated species worldwide. Tuber dry matter content (DMC) and oxidative browning (OxB) are important quality attributes that determine cultivar acceptability in water yam. This study used a single nucleotide polymorphism (SNP) assay from a diversity arrays technology (DArT) platform for a genome-wide association study (GWAS) of the two quality traits in a panel of 100 water yam clones grown in three environments. The marker–trait association analysis identified significant SNPs associated with tuber DMC on chromosomes 6 and 19 and with OxB on chromosome 5. The significant SNPs cumulatively explained 45.87 and 12.74% of the total phenotypic variation for the tuber DMC and OxB, respectively. Gene annotation for the significant SNP loci identified important genes associated in the process of the proteolytic modification of carbohydrates in the dry matter accumulation pathway as well as fatty acid β-oxidation in peroxisome for enzymatic oxidation. Additional putative genes were also identified in the peak SNP sites for both tuber dry matter and enzymatic oxidation with unknown functions. The results of this study provide valuable insight for further dissection of the genetic architecture of tuber dry matter and enzymatic oxidation in water yam. They also highlight SNP variants and genes useful for genomics-informed selection decisions in the breeding process for improving food quality traits in water yam.

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