ANTIOXIDANT POTENTIAL AND OXIDATIVE DNA DAMAGE PREVENTIVE ACTIVITY OFCHRYSANTHEMUM INDICUMEXTRACTS

2012 ◽  
Vol 37 (4) ◽  
pp. 440-448 ◽  
Author(s):  
TRISHNA DEBNATH ◽  
HAI LAN JIN ◽  
MD ABUL HASNAT ◽  
YUNSUK KIM ◽  
NADIRA BINTE SAMAD ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Antioxidant Potential ◽  
Preventive Activity

Oxidative DNA Damage Preventive Activity and Antioxidant Potential ofStevia rebaudiana(Bertoni) Bertoni, a Natural Sweetener

2007 ◽  
Vol 55 (26) ◽  
pp. 10962-10967 ◽  
Author(s):  
Srijani Ghanta ◽  
Anindita Banerjee ◽  
Avijit Poddar ◽  
Sharmila Chattopadhyay
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Antioxidant Potential ◽  
Preventive Activity

scholarly journals Oxidative DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Mehar Darukhshan Kalim ◽  
Dipto Bhattacharyya ◽  
Anindita Banerjee ◽  
Sharmila Chattopadhyay
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Antioxidant Potential ◽  
Preventive Activity

scholarly journals Assessment of Free Radical Scavenging Potential and Oxidative DNA Damage Preventive Activity ofTrachyspermum ammiL. (Carom) andFoeniculum vulgareMill. (Fennel) Seed Extracts

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nandini Goswami ◽  
Sreemoyee Chatterjee
Keyword(s):  
Dna Damage ◽  
Free Radicals ◽  
Free Radical ◽  
Oxidative Dna Damage ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Dpph Radical ◽  
Preventive Activity ◽  
Dpph Radical Scavenging ◽  
Seed Extracts

Oxidation of biomolecules such as carbohydrates, proteins, lipids, and nucleic acids results in generation of free radicals in an organism which is the major cause of onset of various degenerative diseases. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. The present study was designed to examine the free radical scavenging potential and oxidative DNA damage preventive activity of traditionally used spicesTrachyspermum ammiL. (carom) andFoeniculum vulgareMill. (fennel). The aqueous, methanolic, and acetonic extracts ofT. ammiandF. vulgareseeds were prepared using soxhlet extraction assembly and subjected to qualitative and quantitative estimation of phytochemical constituents. Free radical scavenging potential was investigated using standard methods, namely, DPPH radical scavenging assay and ferric reducing antioxidant power assay along with the protection against oxidative DNA damage. The results stated that acetonic seed extracts (AAcSE and FAcSE) of both the spices possessed comparatively high amount of total phenolics whereas methanolic seed extracts (AMSE and FMSE) were found to have highest amount of total flavonoids. At 1 mg/mL concentration, highest DPPH radical scavenging activity was shown by FMSE (96.2%), AAcSE was recorded with highest FRAP value (2270.27 ± 0.005 μmol/L), and all the seed extracts have been shown to mitigate the damage induced by Fenton reaction on calf thymus DNA. Therefore, the study suggests thatT. ammiandF. vulgareseed extracts could contribute as a highly significant bioresource of antioxidants to be used in our day-to-day life and in food and pharmaceutical industry.

scholarly journals Antioxidant potential of Eclipta alba, a traditional medicinal herb attenuates oxidative DNA damage in vitro

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Purushothaman Ayyakkannu ◽  
Ganesh A ◽  
Meenatchi Packirisamy ◽  
Sundaram Ramalingam ◽  
Venkataramanan S
Keyword(s):  
Dna Damage ◽  
Plasmid Dna ◽  
Oxidative Dna Damage ◽  
Antioxidant Activities ◽  
Medicinal Herb ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Documentation System ◽  
Eclipta Alba

Abstract. Purushothaman A, Ganesh A, Meenatchi P, Sundaram R, Venkataramanan. 2020. Antioxidant potential of Eclipta alba, a traditional medicinal herb attenuates oxidative DNA damage in vitro. Nusantara Bioscience 12: 73-78. The plant Eclipta alba (L.) Hassk. is an important plant used in the traditional Ayurvedic, Unani systems of holistic health and herbal medicine of the East. This study aimed to evaluate the antioxidant and DNA damage protection activities of ethanolic extract of E. alba. Quantitative analysis of total phenolic content (TPC) and identification of bioactive components using Gas Chromatography-Mass Spectroscopy (GC-MS) was performed to provide scientific basis for traditional usage of this plant. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH·), hydrogen peroxide (H2O2) and Superoxide radicals (O2•-). DNA damage protective activity of ethanol extract of E. alba was checked on pBluescript M13+ plasmid DNA. The Plasmid DNA was oxidized with H2O2 + UV treatment in the absence and presence of different concentrations of E. alba extract (75, 150, and 300 μg/mL). Electrophoresis was performed using 1% agarose at 40 V for 3 h in the presence of ethidium bromide. Gel was scanned on a Gel documentation system. Bands on the gels corresponding to supercoiled circular, circular relaxed, and linearized DNA were quantified. The results of preliminary phytochemical screening of E. alba extract showed the presence of flavonoids, saponins, steroids, terpenoids, and tannins. The extract was found to have rich phenolics content of 26.38 ± 2.45 milligram of gallic acid equivalents (mg GAE/g). The extract exhibited excellent antioxidant activities. GC-MS analysis of the extract confirmed the presence of major active principles. Furthermore, the extract significantly inhibited DNA damage induced by reactive oxygen species (ROS). Altogether, the results of current study revealed that E. alba is a potential source of antioxidants and provides pharmacological credibility to the ethnomedicinal use of this plant in traditional system of medicine, also justifying its therapeutic application in oxidative damage induced diseases such as cancer, diabetes, and neurological disorders.

scholarly journals In vitro Antioxidant Potential and Oxidative DNA Damage Protecting Activity of the Ethanol Extracts of Cacalia firma Komar

2011 ◽  
Vol 54 (4) ◽  
pp. 258-264 ◽  
Author(s):  
Jin-Ha Lee ◽  
Bong-Gyeong Lee ◽  
Ae-Ri Park ◽  
Kye-Jhae Lee ◽  
Dae-Woon Choi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Antioxidant Potential ◽  
Ethanol Extracts

Acrylamide Exposure and Oxidative DNA Damage, Lipid Peroxidation and Fasting Plasma Glucose Alteration: Association and Mediation Analyses in Chinese Urban Adults

2020 ◽  
Author(s):  
Bin Wang ◽  
Weihong Qiu ◽  
Shijie Yang ◽  
Limin Cao ◽  
Chunmei Zhu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Plasma Glucose ◽  
Fasting Plasma Glucose ◽  
Oxidative Dna Damage ◽  
Adult Population ◽  
Prostaglandin F2α ◽  
Mediation Analyses ◽  
Mediating Role ◽  
Fasting Plasma

<a><b>OBJECTIVE: </b></a>Acrylamide exposure from daily-consumed food has raised global concern.<b> </b>We aimed to assess the exposure-response relationships of internal acrylamide exposure with oxidative DNA damage, lipid peroxidation and fasting plasma glucose (FPG) alteration, and investigate the mediating role of oxidative DNA damage and lipid peroxidation in the association of internal acrylamide exposure with FPG. <p><b>RESEARCH DESIGN AND METHODS:</b> FPG and urinary biomarkers of oxidative DNA damage (8-hydroxy-deoxy-guanosine, 8-OHdG), lipid peroxidation (8-iso-prostaglandin-F2α, 8-iso-PGF2α) and acrylamide exposure (N-acetyl-S-(2-carbamoylethyl)-L-cysteine, AAMA; N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine, GAMA) were measured for 3,270 general adults from the Wuhan-Zhuhai cohort. The associations of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG were assessed by linear mixed models. The mediating roles of 8-OHdG and 8-iso-PGF2α were evaluated by mediation analysis.</p> <p><b>RESULTS:</b> We found significant linear positive dose-response relationships of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG (except GAMA with FPG), and 8-iso-PGF2α with FPG. Each 1-unit increase in log-transformed level of AAMA, ΣUAAM (AAMA+GAMA) or 8-iso-PGF2α was associated with a 0.17-, 0.15- or 0.23-mmol/L increase in FPG, respectively (<i>P </i>or/and<i> P trend</i><0.05). Each 1% increase in AAMA, GAMA or ΣUAAM was associated with a 0.19%, 0.27% or 0.22% increase in 8-OHdG, respectively, and a 0.40%, 0.48% or 0.44% increase in 8-iso-PGF2α, respectively (<i>P </i>and<i> P trend</i><0.05). Increased 8-iso-PGF2α rather than 8-OHdG significantly mediated 64.29% and 76.92% of the AAMA and ΣUAAM associated-FPG increases, respectively.</p> <p><b>CONCLUSIONS:</b> Exposure of general adult population to acrylamide was associated with FPG elevation, oxidative DNA damage and lipid peroxidation, which in turn partly mediated acrylamide-associated FPG elevation.<b></b></p>

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