Preparation of low degree of substitution octenyl succinic starch ester by response surface methodology and its property analysis

This work shows an optimized enzymatic hydrolysis of high molecular weight potato galactan yielding pectic galactan-oligosaccharides (PGOs), where endo-β-1,4-galactanase (galactanase) from Cellvibrio japonicus and Clostridium thermocellum was used. For this, response surface methodology (RSM) by central composite design (CCD) was applied. The parameters varied were temperature (°C), pH, incubation time (min), and enzyme/substrate ratio (U/mg). The optimized conditions for the production of low degree of polymerization (DP) PGOs were obtained for each enzyme by spectrophotometric assay and confirmed by chromatography. The optimal conditions predicted for the use of C. japonicus galactanase to obtain PGOs of DP = 2 were T = 51.8 °C, pH 5, E/S = 0.508 U/mg, and t = 77.5 min. For DP = 3, they were T = 21 °C, pH 9, E/S = 0.484 U/mg, and t = 12.5 min; and for DP = 4, they were T = 21 °C, pH 5, E/S = 0.462 U/mg, and t = 12.5 min. The efficiency results were 51.3% for substrate hydrolysis. C. thermocellum galactanase had a lower yield (35.7%) and optimized conditions predicted for PGOs of DP = 2 were T = 60 °C, pH 5, E/S = 0.525 U/mg, and time = 148 min; DP = 3 were T = 59.7 °C, pH 5, E/S = 0.506 U/mg, and time = 12.5 min; and DP = 4, were T = 34.5 °C, pH 11, E/S = 0.525 U/mg, and time = 222.5 min. Fourier transformed infrared (FT-IR) and nuclear magnetic resonance (NMR) characterizations of PGOs are presented.

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Optimasi Sintesis Karboksi Metil Selulosa (CMC) dari Pelepah Kelapa Sawit Menggunakan Response Surface Methodology (RSM)

Jurnal Agritech ◽

10.22146/agritech.25363 ◽

2017 ◽

Vol 37 (2) ◽

pp. 158

Author(s):

M. Khoiron Ferdiansyah ◽

Djagal Wiseso Marseno ◽

Yudi Pranoto

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Methyl Cellulose ◽

Degree Of Substitution ◽

Raw Material ◽

Carboxy Methyl Cellulose ◽

Optimum Conditions ◽

Material Synthesis ◽

Verification Test ◽

Carboxy Methyl

Palm midrib contain 89.63 % of cellulose. Cellulose is the main raw material synthesis of carboxy methyl cellulose (CMC). The purpose of this research was to determine the optimum conditions of carboxy methyl cellulose (CMC) synthesis from palm midrib. In this research, the concentration of NaOH, NaMCA weight, and the temperature of carboxymethylation reaction were examined. The response optimized on the CMC was the degree of substitution (DS). The optimum conditions of CMC synthesis from palm midrib cellulose was obtained from 10 % of NaOH, 4.57 g of NaMCA, and the reaction temperature of 46.59 °C. Response Surface Methodology calculation showed that CMC with optimum condition had the degree of substitution (DS) value of 0.83, while in the verification test the DS value was 0.75. ABSTRAKPelepah kelapa sawit mempunyai kandungan selulosa sebesar 89,63 %. Selulosa merupakan bahan baku utama sintesis karboksi metil selulosa (CMC). Tujuan dari penelitian ini adalah untuk mengetahui kondisi optimum sintesis CMC dari pelepah kelapa sawit. Faktor yang diteliti pada penelitian ini adalah konsentrasi NaOH, berat NaMCA, dan suhu reaksi karboksimetilasi. Respon yang dioptimasi pada CMC yang dihasilkan adalah derajat substitusi (DS). Kondisi optimum sintesis CMC dari selulosa pelepah kelapa sawit didapatkan dengan konsentrasi NaOH 10 %, berat NaMCA 4,57 g, dan suhu reaksi 46,59 °C. Hasil dari perhitungan RSM menunjukkan CMC dengan kondisi optimum memiliki nilai DS sebesar 0,83 sedangkan uji verifikasi menunjukkan nilai DS sebesar 0,75.

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Optimasi Sintesis Methyl Cellulose (MC) dari Biji Salak (Salacca edulis Reinw) Pondoh Super

Journal of Science and Application Technology ◽

10.35472/jsat.v5i1.389 ◽

2021 ◽

Vol 5 (1) ◽

pp. 78

Author(s):

Amalia Wahyuningtyas ◽

Agus Setyoko ◽

Sri Anggrahini ◽

Djagal Wiseso Marseno

Keyword(s):

Response Surface Methodology ◽

Optimum Condition ◽

Response Surface ◽

Dimethyl Sulfate ◽

Methyl Cellulose ◽

Temperature Treatment ◽

Food Additive ◽

Degree Of Substitution ◽

Crystalline Region ◽

Fruit Species

Snake fruit (Salacca zalacca) is one of the typical fruits from Indonesia. Pondoh is one of the snake fruit species largely found in Yogyakarta. The kernels of snake fruit are wastes, but contains a lot of cellulose. Cellulose can be processed into methyl cellulose And be used as food additive. This study aimed to understand the optimized the optimizing synthesis of methyl cellulose through the concentration of NaOH, dimethyl sulfate and reaction temperature. The study was conducted by extracting cellulose using 4 % NaOH concentrations. Dehemicellulose and bleaching were done to dilute hemicellulose and lignin. The optimization of methyl cellulose was done using Response Surface Methodology with various concentrations of NaOH (10, 15, 20, 25, 30%), dimethyl sulfate (2, 3, 4, 5, 6 ml), and temperature (45, 50, 55, 60, 65°C). NaOH could change cellulose crystalline region and formed Na-cellulose. Dimethyl sulfate can be acted as substitution agent, while temperature controlling plays role to obtain appropriate conditions for reaction. The results showed that the use of NaOH concentration, dimethyl sulfate and temperature affected the degree of substitution, OHC and lightness. The addition of NaOH and dimethyl sulfate in producing methyl cellulose had more effect on the increasing of degree of substitution than temperature treatment. The optimum condition of methyl cellulose were 0.91% NaOH; 3.52 ml of dimethyl sulfate, at 46.51°C temperature.

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Preparation and Optimization of Water-Soluble Cationic Sago Starch with a High Degree of Substitution Using Response Surface Methodology

Polymers ◽

10.3390/polym12112614 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2614

Author(s):

Nur’Izzah Md Nasir ◽

Emilia Abdulmalek ◽

Norhazlin Zainuddin

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Reaction Times ◽

Degree Of Substitution ◽

Water Soluble ◽

Trimethylammonium Chloride ◽

Sago Starch ◽

X Ray Diffraction ◽

High Degree ◽

Central Composite

Modification and characterizations of cationic sago starch with 3-chloro-2-hydroxypropyl trimethylammonium chloride (CHPTAC) prepared via etherification reaction was reported in this study. The optimization of cationic sago starch modification was performed by utilizing the combination of response surface methodology and central composite design (RSM/CCD). The effect of each variable and the interaction between the three variables, the concentration of CHPTAC, concentration of the catalyst NaOH, and the reaction times on the degree of substitution (DS) of the product were investigated and modeled. Moderate conditions were employed and a water-soluble cationic sago starch with high DS value was obtained. Based on RSM, the highest DS = 1.195 was obtained at optimum conditions: 0.615 mol of CHPTAC concentration (CHPTAC/SS = 5), 30% w/v NaOH, and 5 h reaction time, at 60 °C reaction temperature. Furthermore, the cationic sago starch was characterized using Fourier transform infrared spectroscopy, FTIR, X-ray diffraction, XRD, and field emission scanning electron microscopy, FESEM.

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