Reply to “Misuse of molecular tools results in misleading dates for the ancestor of the Indo‐Pacific humpback dolphin” by Chen

2022 ◽  
Vol 38 (1) ◽  
pp. 395-399
Author(s):  
Liyuan Zhao ◽  
Watchara Sakornwimon ◽  
Wenzhi Lin ◽  
Peijun Zhang ◽  
Rachawadee Chantra ◽  
...  
Keyword(s):  
Molecular Tools

Molecular Mechanisms Underlying the Functions of Cellular Markers Associated with the Phenotype of Cancer Stem Cells

2019 ◽  
Vol 14 (5) ◽  
pp. 405-420 ◽  
Author(s):  
Eduardo Alvarado-Ortiz ◽  
Miguel Á. Sarabia-Sánchez ◽  
Alejandro García-Carrancá
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Molecular Mechanisms ◽  
Tumor Biology ◽  
Molecular Tools ◽  
Recent Past ◽  
Functional Roles ◽  
Cellular Population ◽  
Cellular Markers ◽  
A Minor

Cancer Stem Cells (CSC) generally constitute a minor cellular population within tumors that exhibits some capacities of normal Stem Cells (SC). The existence of CSC, able to self-renew and differentiate, influences central aspects of tumor biology, in part because they can continue tumor growth, give rise to metastasis, and acquire drug and radioresistance, which open new avenues for therapeutics. It is well known that SC constantly interacts with their niche, which includes mesenchymal cells, extracellular ligands, and the Extra Cellular Matrix (ECM). These interactions regularly lead to homeostasis and maintenance of SC characteristics. However, the exact participation of each of these components for CSC maintenance is not clear, as they appear to be context- or cell-specific. In the recent past, surface cellular markers have been fundamental molecular tools for identifying CSC and distinguishing them from other tumor cells. Importantly, some of these cellular markers have been shown to possess functional roles that affect central aspects of CSC. Likewise, some of these markers can participate in regulating the interaction of CSC with their niche, particularly the ECM. We focused this review on the molecular mechanisms of surface cellular markers commonly employed to identify CSC, highlighting the signaling pathways and mechanisms involved in CSC-ECM interactions, through each of the cellular markers commonly used in the study of CSC, such as CD44, CD133, CD49f, CD24, CXCR4, and LGR5. Their presence does not necessarily implicate them in CSC biology.

Applying Molecular Tools for Monitoring Inhibition of Nitrification by Heavy Metals

2015 ◽  
Vol 2015 (10) ◽  
pp. 2825-2826 ◽  
Author(s):  
Vikram Kapoor ◽  
Xuan Li ◽  
Christopher A Impellitteri ◽  
Kartik Chandran ◽  
Jorge W Santo Domingo
Keyword(s):  
Heavy Metals ◽  
Molecular Tools ◽  
Inhibition Of Nitrification

Molecular Tools Developed for Disease Resistant Genes in Wheat, Barley, Lentil and Chickpea: AReview

2018 ◽  
Vol 36 (1) ◽  
pp. 050-056 ◽  
Author(s):  
Aladdin Hamawieh ◽  
◽  
Fida Alo ◽  
Seid Ahmed ◽  
◽  
...  
Keyword(s):  
Molecular Tools ◽  
Resistant Genes ◽  
Disease Resistant

scholarly journals Measurements Methods for the Development of MicroRNA-Based Tests for Cancer Diagnosis

2021 ◽  
Vol 22 (3) ◽  
pp. 1176
Author(s):  
Francesca Precazzini ◽  
Simone Detassis ◽  
Andrea Selenito Imperatori ◽  
Michela Alessandra Denti ◽  
Paola Campomenosi
Keyword(s):  
Expression Profiling ◽  
Clinical Stage ◽  
Response To Therapy ◽  
Clinical Settings ◽  
Molecular Tools ◽  
Altered Expression ◽  
Circulating Micrornas ◽  
Non Invasive ◽  
Mirna Expression Profiling ◽  
Technological Limitations

Studies investigating microRNAs as potential biomarkers for cancer, immune-related diseases, or cardiac pathogenic diseases, among others, have exponentially increased in the last years. In particular, altered expression of specific miRNAs correlates with the occurrence of several diseases, making these molecules potential molecular tools for non-invasive diagnosis, prognosis, and response to therapy. Nonetheless, microRNAs are not in clinical use yet, due to inconsistencies in the literature regarding the specific miRNAs identified as biomarkers for a specific disease, which in turn can be attributed to several reasons, including lack of assay standardization and reproducibility. Technological limitations in circulating microRNAs measurement have been, to date, the biggest challenge for using these molecules in clinical settings. In this review we will discuss pre-analytical, analytical, and post-analytical challenges to address the potential technical biases and patient-related parameters that can have an influence and should be improved to translate miRNA biomarkers to the clinical stage. Moreover, we will describe the currently available methods for circulating miRNA expression profiling and measurement, underlining their advantages and potential pitfalls.

scholarly journals Fraud in highly appreciated fish detected from DNA in Europe may undermine the Development Goal of sustainable fishing in Africa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carmen Blanco-Fernandez ◽  
Alba Ardura ◽  
Paula Masiá ◽  
Noemi Rodriguez ◽  
Laura Voces ◽  
...  
Keyword(s):  
Sustainable Consumption ◽  
Pcr Amplification ◽  
Molecular Tools ◽  
Food Authenticity ◽  
Production And Consumption ◽  
Fish Samples ◽  
Sustainable Seafood ◽  
The Relationship ◽  
Unregulated Fishing ◽  
High Effort

AbstractDespite high effort for food traceability to ensure safe and sustainable consumption, mislabeling persists on seafood markets. Determining what drives deliberate fraud is necessary to improve food authenticity and sustainability. In this study, the relationship between consumer’s appreciation and fraudulent mislabeling was assessed through a combination of a survey on consumer’s preferences (N = 1608) and molecular tools applied to fish samples commercialized by European companies. We analyzed 401 samples of fish highly consumed in Europe and worldwide (i.e. tuna, hake, anchovy, and blue whiting) through PCR-amplification and sequencing of a suite of DNA markers. Results revealed low mislabeling rate (1.9%), with a higher mislabeling risk in non-recognizable products and significant mediation of fish price between consumer´s appreciation and mislabeling risk of a species. Furthermore, the use of endangered species (e.g. Thunnus thynnus), tuna juveniles for anchovy, and still not regulated Merluccius polli hake as substitutes, points towards illegal, unreported and/or unregulated fishing from African waters. These findings reveal a worrying intentional fraud that hampers the goal of sustainable seafood production and consumption, and suggest to prioritize control efforts on highly appreciated species.

scholarly journals Detection of Rickettsia raoultii in Dermacentor reticulatus and Haemaphysalis inermis ticks in Slovakia

Biologia ◽  
2021 ◽  
Author(s):  
Basma Ouarti ◽  
Basma El Hamzaoui ◽  
Michal Stanko ◽  
Maureen Laroche ◽  
Oleg Mediannikov ◽  
...  
Keyword(s):  
Potential Role ◽  
Animal Health ◽  
Molecular Study ◽  
Dermacentor Reticulatus ◽  
Experimental Models ◽  
Molecular Tools ◽  
Future Studies ◽  
Rickettsia Raoultii ◽  
Pathogenic Agents ◽  
Transmission Models

AbstractTicks are vector arthropods responsible for the transmission of several pathogenic agents that affect both human and animal health worldwide. In this study our objective was to analyse, using molecular tools, the bacterial community of Dermacentor reticulatus and Haemaphysalis inermis ticks collected in south-eastern Slovakia. Using real-time PCR, we identified the presence of Rickettsia spp. DNA at levels of 14/59 (23.72 %) and 29/173 (16.76 %) in D. reticulatus and H. inermis, respectively. In addition, using standard PCR and sequencing, we identified the presence of Rickettsia raoultii DNA in 13 ticks belonging to the two investigated species. Rickettsia raoultii blast results revealed an average identification percentage of 99.62 %. Following the results of this molecular study there is a possibility that D. reticulatus and H. inermis play a potential role in the transmission of R. raoultii. To prove the possibility of validity of this hypothesis, we suggest performing experimental models in future studies. Our results can serve as preliminary data for future transmission models.

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