Effects of heat shock and ambient temperature on female soldier production in a polyembryonic parasitic wasp

sHSP (small heat-shock protein) IbpB (inclusion-body-binding protein B) from Escherichia coli is known as an ATP-independent holding chaperone which prevents the insolubilization of aggregation-prone proteins by forming stable complexes with them. It was found that the chaperone function of IbpB is greatly modulated by the ambient temperature, i.e. when the temperature increases from normal to heat-shock, the chaperone activity of IbpB is dramatically elevated to a level that allows it to effectively bind the aggregation-prone client proteins. Although it is generally believed that the release and refolding of the client protein from the sHSPs depends on the aid of the ATP-dependent chaperones such as Hsp (heat-shock protein) 70 and Hsp100 when the ambient temperature recovers from heat-shock to normal, the behaviour of the sHSPs during this recovery stage has not yet been investigated. In the present study, we examined the behaviour and properties of IbpB upon temperature decrease from heat-shock to normal. We found that IbpB, which becomes functional only under heat-shock conditions, retains the chaperone activity for an extended period of time after the heat-shock stress condition is removed. A detail comparison demonstrates that such preconditioned IbpB is distinguished from the non-preconditioned IbpB by a remarkable conformational transformation, including a significant increase in the flexibility of the N- and C-terminal regions, as well as enhanced dynamic subunit dissociation/reassociation. Intriguingly, the preconditioned IbpB displayed a dramatic decrease in its surface hydrophobicity, suggesting that the exposure of hydrophobic sites might not be the sole determinant for IbpB to exhibit chaperone activity. We propose that the maintenance of the chaperone activity for such ‘holdases’ as sHSPs would be important for cells to recover from heat-shock stress.

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Comparison of morphology, development and expression patterns of hsf and hsp11.0 of Cotesia chilonis under normal and high temperature

PeerJ ◽

10.7717/peerj.11353 ◽

2021 ◽

Vol 9 ◽

pp. e11353

Author(s):

Fu-Jing He ◽

Feng Zhu ◽

Ming-Xing Lu ◽

Yu-Zhou Du

Keyword(s):

High Temperature ◽

Heat Shock ◽

Developmental Stages ◽

Expression Patterns ◽

Parasitic Wasp ◽

Developmental Duration ◽

Developmental Indicators ◽

Rice Pest ◽

And Control ◽

Cotesia Chilonis

Cotesia chilonis (Munakata) is the dominant parasitic wasp of the rice pest, Chilo suppressalis (Walker), and is a valuable parasitic wasp for the prevention and control of C. suppressalis. In this study, developmental indicators and expression of Cchsp11.0 (heat shock protein 11.0) and Cchsf (heat shock factor) were compared for C. chilonis at 27 °C and 36 °C. Developmental duration, morphology, emergence rate, and number of C. chilonis offspring were shortened at 36 °C while the ratio of females to males increased. Cchsp11.0 and Cchsf were highly expressed in the 1st instar stage at 36 °C, and Cchsp11.0 expression gradually decreased as C. chilonis matured; Cchsf expression was not correlated with Cchsp11.0 expression. Compared with 27 °C, the expression pattern of Cchsp11.0 and Cchsf was also not consistent, and Cchsp11.0 expression increased significantly at the adult stage. In conclusion, mildly high temperatures impact growth, development and reproduction of C. chilonis and stimulate the expression of Cchsp11.0 and Cchsf, and Cchsp11.0 and Cchsf play different roles in different developmental stages of C. chilonis at normal and high temperature.

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Genetic selection for growth performance and thermal tolerance under high ambient temperature after two generations using heat shock protein 90 expression as an index

Animal Production Science ◽

10.1071/an17746 ◽

2019 ◽

Vol 59 (4) ◽

pp. 628 ◽

Cited By ~ 3

Author(s):

Lamiaa M. Radwan ◽

Mahmoud. Y. Mahrous

Keyword(s):

Heat Stress ◽

Heat Shock ◽

Heat Shock Protein ◽

Ambient Temperature ◽

Rhode Island ◽

Genetic Selection ◽

Heat Shock Protein 90 ◽

High Ambient Temperature ◽

Two Generations ◽

Selection For

Genetic selection for productive performance in high ambient temperatures was performed on two chicken strains, Rhode Island Red and Sinai, for two generations, and the heritable responses to tolerance were estimated using heat shock protein 90 (HSP90) gene expression. The results are summarised as follows: (1) heat stress negatively affected some economic traits, mainly bodyweight. This effect was more pronounced in the parent stock than in the first generation (F1) and second generation (F2). (2) This effect was modulated by the chicken strain, and the decreased bodyweight was more pronounced in RI strain than in the Sinai strain, indicating that the Sinai strain is more tolerant to high ambient temperature. (3) The offspring (F1 and F2) of both strains were more tolerant to high ambient temperature; this trend was also true for the parents of these two strains. (4) HSP90 mRNA expression was the same in both strains under normal conditions in all three generations. (5) Under high ambient temperature conditions, the Sinai strain (all generations) showed significantly increased HSP90 mRNA expression compared with the Rhode Island Red strain. These findings suggest that heat tolerance is passed from parents to offspring. We recommended that selection for heat-stress tolerance be applied to producing commercial strains reared in hot climate conditions.

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Low ambient temperature reveals distinct mechanisms for MDMA-induced serotonergic toxicity and astroglial Hsp27 heat shock response in rat brain

Neurochemistry International ◽

10.1016/j.neuint.2011.06.017 ◽

2011 ◽

Vol 59 (5) ◽

pp. 695-705 ◽

Cited By ~ 3

Author(s):

Csaba Ádori ◽

Rómeó D. Andó ◽

Tamás Balázsa ◽

Csaba Sőti ◽

Szilvia Vas ◽

...

Keyword(s):

Heat Shock ◽

Ambient Temperature ◽

Rat Brain ◽

Heat Shock Response ◽

Shock Response ◽

Low Ambient Temperature

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SEM of non-coated hepatocellular cytoskeleton of perfused livers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111641 ◽

1984 ◽

Vol 42 ◽

pp. 306-307

Author(s):

S.W. French ◽

N.C. Benson ◽

C. Davis-Scibienski

Keyword(s):

Ambient Temperature ◽

Critical Point ◽

Protease Inhibitors ◽

Metal Deposition ◽

Heat Damage ◽

Detergent Extraction ◽

Cytoskeletal Elements ◽

Triton X ◽

Excised Tissue

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.

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Formation of Dislocation Channels in Neutron Irradiated Molybdenum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100096618 ◽

1972 ◽

Vol 30 ◽

pp. 672-673

Author(s):

S. Mahajan

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Ambient Temperature ◽

Room Temperature ◽

Channel Formation ◽

Early Stages ◽

Transmission Electron ◽

Three Stages ◽

Two Stages ◽

Polycrystalline Molybdenum

The evolution of dislocation channels in irradiated metals during deformation can be envisaged to occur in three stages: (i) formation of embryonic cluster free regions, (ii) growth of these regions into microscopically observable channels and (iii) termination of their growth due to the accumulation of dislocation damage. The first two stages are particularly intriguing, and we have attempted to follow the early stages of channel formation in polycrystalline molybdenum, irradiated to 5×1019 n. cm−2 (E > 1 Mev) at the reactor ambient temperature (∼ 60°C), using transmission electron microscopy. The irradiated samples were strained, at room temperature, up to the macroscopic yield point.Figure 1 illustrates the early stages of channel formation. The observations suggest that the cluster free regions, such as A, B and C, form in isolated packets, which could subsequently link-up to evolve a channel.

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Crystallographic Characterization of Dislocation Loops in Irradiated Tungsten

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100101190 ◽

1968 ◽

Vol 26 ◽

pp. 290-291

Author(s):

Robert C. Rau

Keyword(s):

Electron Microscope ◽

Ambient Temperature ◽

Dislocation Loops ◽

Polycrystalline Specimen ◽

Post Irradiation ◽

Neutron Fluences

Previous work has shown that post-irradiation annealing, at temperatures near 1100°C, produces resolvable dislocation loops in tungsten irradiated to fast (E > 1 MeV) neutron fluences of about 4 x 1019 n/cm2 or greater. To crystallographically characterize these loops, tilting experiments were carried out in the electron microscope on a polycrystalline specimen which had been irradiated to 1.5 × 1021 n/cm2 at reactor ambient temperature (∼ 70°C), and subseouently annealed for 315 hours at 1100°C. This treatment produced large loops averaging 1000 Å in diameter, as shown in the micrographs of Fig. 1. The orientation of this grain was near (001), and tilting was carried out about axes near [100], [10] and [110].

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