scholarly journals The detection and phylogenetic analysis of porcine deltacoronavirus from Guangdong Province in Southern China

2017 ◽  
Vol 65 (1) ◽  
pp. 166-173 ◽  
Author(s):  
K. Mai ◽  
J. Feng ◽  
G. Chen ◽  
D. Li ◽  
L. Zhou ◽  
...  
Zootaxa ◽  
2017 ◽  
Vol 4337 (3) ◽  
pp. 423 ◽  
Author(s):  
YI-TAO LIN ◽  
WEI-TAO FENG ◽  
FAN XIN ◽  
LV ZHANG ◽  
YU ZHANG ◽  
...  

Two new species of Macrostomum (Platyhelminthes: Macrostomorpha), M. shiyanensis n. sp. and M. lankouensis n. sp. are described from Shiyan Reservoir and Lankou Town. Morphological characteristics of the penis stylets and sperm, as well as the phylogenetic analysis using concatenated sequences of 18S and 28S rDNA genes support the establishment of these two new species and indicate that they are closely related to M. sinensis and M. heyuanensis described from different regions of Guangdong province. Finally, the progress of molecular phylogenetics of Macrostomum worldwide is also discussed. 


PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253622
Author(s):  
Feng Wen ◽  
Jing Yang ◽  
Anqi Li ◽  
Zhonggui Gong ◽  
Lulu Yang ◽  
...  

Porcine epidemic diarrhea virus (PEDV), a leading cause of piglet diarrhea outbreaks, poses a significant danger to the swine industry. The aim of this study was to investigate the epidemic characteristics of PEDV that was circulating in Guangdong province, one of China’s major pig producing provinces. Clinical samples were collected from eight pig farms in Guangdong province between 2018 and 2019 and tested for the major porcine enteric pathogens, including PEDV, transmissible gastroenteritis virus (TGEV), Swine enteric coronavirus (SeCoV), Swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine deltacoronavirus (PDCoV), and porcine rotavirus (RV). As a result, only PEDV and RV were detected at a rate of 47.0% (16/34) and 18.6% (8/34), respectively. Coinfectoin with PEDV and RV occurred at a rate of PEDV 12.5% (2/16). Subsequently, the full-length S gene sequences of 13 PEDV strains were obtained, and phylogenetic analysis suggested the presence of GII-c group PEDV strains in this region (non-S-INDEL). Two novel common amino acid insertions (55T/IG56 and 551L) and one novel glycosylation site (1199G+) were detected when the CV777 and ZJ08 vaccine strains were compared. Furthermore, intragroup recombination events in the S gene regions 51–548 and 2478–4208 were observed in the PEDV strains studied. In summary, the observations provide current information on the incidence of viral agents causing swine diarrhea in southern China and detailed the genetic characteristics and evolutionary history of the dominant PEDV field strains. Our findings will aid in the development of an updated vaccine for the prevention and control of PEDV variant strains.


Phytotaxa ◽  
2018 ◽  
Vol 364 (3) ◽  
pp. 227 ◽  
Author(s):  
GENG-SHEN WANG ◽  
YU SONG ◽  
JING-WEI LI ◽  
SHI-YAO XIA ◽  
LI-HONG QIU

Two new species of Lactarius from Dinghu Mountain, Guangdong Province in southern China were described based on both morphological characters and phylogenetic analysis of internal transcribed spacer (ITS) sequences. Lactarius verrucosporus sp. nov. is characterized by reddish brown pileus with white and decurved margin, pale cream lamellae, globose to subglobose basidiospores with isolated warts, thin pileipellis composed of globose subterminal cells and inflated terminal elements. Lactarius nigricans sp. nov. is recognized by greyish brown pileus, beige lamellae, amyloid basidiospores with high ridges, subcylindrical to subfusiform caulocystidia, thick pileipellis with long terminal cells. Both molecular and morphological analysis consistently confirm that L. verrucosporus and L. nigricans belong to Lactarius subgenera Russularia and Plinthogalus, respectively, and both species are distinct from all described species of these two subgenera. The morphological differences of these two novel species with their closely related taxa are discussed.


Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 728
Author(s):  
Bao-Gui Jiang ◽  
Ai-Qiong Wu ◽  
Jia-Fu Jiang ◽  
Ting-Ting Yuan ◽  
Qiang Xu ◽  
...  

A novel Borrelia species, Candidatus Borrelia javanense, was found in ectoparasite ticks, Amblyomma javanense, from Manis javanica pangolins seized in anti-smuggling operations in southern China. Overall, 12 tick samples in 227 (overall prevalence 5.3%) were positive for Candidatus B. javanense, 9 (5.1%) in 176 males, and 3 (5.9%) in 51 females. The phylogenetic analysis, based on the 16S rRNA gene and the flagellin gene sequences of the Borrelia sp., exhibited strong evidence that Candidatus B. javanense did not belong to the Lyme disease Borrelia group and the relapsing fever Borrelia group but another lineage of Borrelia. The discovery of the novel Borrelia species suggests that A. javanense may be the transmit vector, and the M. javanica pangolins should be considered a possible origin reservoir in the natural circulation of these new pathogens. To our knowledge, this is the first identification of a novel Borrelia species agent in A. javanense from pangolins. Whether the novel agent is pathogenic to humans is unknown and needs further research.


Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 666 ◽  
Author(s):  
Juyang Liao ◽  
Linping Zhang ◽  
Yan Liu ◽  
Qiaoyun Li ◽  
Danxia Chen ◽  
...  

Food supply from forests is a fundamental component of forest ecosystem services, but information relating to suitability for human consumption and sustainable utilization of non-timber forest products (NTFPs) in developing countries is lacking. To address this gap in knowledge, diverse datasets of edible plants and macro-fungi were obtained from field collections, historical publications, and community surveys across seven cities in Guangdong Province (GP), southern China. Seven edible parts and five food categories of plant species were classified according to usage and specific nutrient components. Edible plant species were also categorized into different seasons and life forms. Our results show that at least 100 plant species (with 64 plant species producing fruit) and 20 macro-fungi were commonly used as edible forest products in subtropical GP. There were 55 and 57 species providing edible parts in summer and autumn, respectively. Many edible plants had multiple uses. Tree and herbaceous species made up the majority of the total. Our study provides evidence that both edible plants and macro-fungi can enrich the food supply for residents in rural and urban areas by acting as supplemental resources. We therefore suggest that, in spite of the prevalence of imported foods due to modern infrastructure, edible NTFPs from subtropical forests might be leveraged to support the increasing demand for food in an era of rapid urbanization and global change.


2019 ◽  
Author(s):  
Hai-Yan Ye ◽  
Fan-Fan Xing ◽  
Jin Yang ◽  
Simon Kam-Fai Lo ◽  
Ricky Wing-Tong Lau ◽  
...  

Abstract Background: Brucellosis is one of the most widespread zoonosis in the world. In China, 90% of human brucellosis occurs in six northern agricultural provinces. However, there is a recent increase in the trend of human brucellosis in southern provinces with limited cases reported in the literature. Our study aims to describe the clinical features and epidemiology of brucellosis in a tertiary hospital in southern China. Methods: A retrospective case series of brucellosis was conducted between January 1, 2014 and October 31. 2018. Cases were identified based on positive Brucella serology by tube agglutination test, or positive culture from clinical specimen identified by Vitek 2 and MALDL-TOF MS. Clinical details of brucellosis including patients’ occupation, risk factors, and complications were analyzed. Clinical characteristics between patients from Guangdong and other provinces were also compared. Results: A total of 13 cases of laboratory-confirmed brucellosis were identified. 7 (53.8%) of the patients were male, 6 (46.2%) were female, with age ranging from 29 to 73 years old (median age: 51 years). Five patients (38.5%) were from Guangdong province, while the remaining patients (61.5%) were from other provinces. The commonest risk factors of acquisition were consumption of undercooked meat and goat placenta. Patients from Guangdong province were found to be more likely to have prior placenta consumption and handling of animal meat. The commonest clinical presentations were fever, osteoarticular pain, urinary symptoms, splenomegaly, and lymphadenopathy. Spondylodiscitis/ peripheral joint arthritis (5 patients, 38.5%) was the most prevalent complication, while extra-osteoarticular complications including abdominal aortitis, hepatosplenic abscess, chest wall abscess, and epididymo-orchitis were observed in 4 other patients. Furthermore, it was demonstrated that MALDI-TOF MS is reliable in Brucella identification after additional of reference spectra with standard Brucella strain. Conclusions: Brucellosis, previously thought to be only found in northern China, is now increasingly seen in highly cosmopolitan part of southern China. MALDI-TOF MS in hospitals in China should include reference spectra with standard Brucella strain to aid bacterial identification in routine clinical practice. In addition to tuberculosis, typhoid fever and typhus, brucellosis should be considered in patients with fever of unknown origin in this locality.


MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 103-139
Author(s):  
Guo-Jie Li ◽  
Shou-Mian Li ◽  
Bart Buyck ◽  
Shi-Yi Zhao ◽  
Xue-Jiao Xie ◽  
...  

Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R. straminella, R. subpectinatoides and R. succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R. indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.


2020 ◽  
Author(s):  
Jianbo Li ◽  
Weihua Fan ◽  
Yueshan Zhou ◽  
Linmei Wu ◽  
Wei Liu ◽  
...  

Abstract Background: Dental caries of deciduous teeth (Early Childhood Caries, ECC) has become a crucial oral health problem over the decades in China. The aim of this study was to examine the prevalence and severity of ECC among preschool children from Guangdong Province, Southern China. In addition, to assess the association of ECC with reported oral health-related behaviors.Methods: A cross-sectional survey was carried out in Guangdong Province by means of an equal-sized, stratified, multistage random sampling method. According to the standard for clinical dentition examination of the WHO 2013 criteria, the presence of ECC was determined by the dmft (decayed-missing-filled tooth) index using a CPI (Community Periodontal Index) probe. A questionnaire about caries-related factors was completed by each of the participants’ parents or grandparents through a face-to-face and one-on-one interview. Then, t-test, Chi2 tests, One-Way ANOVA served for statistical analysis, and logistic regression analysis as well as covariance analysis were executed to identify potential associated factors for ECC.Results: There were 2592 3- to 5-year-old preschool children. The prevalence (% dmft >0) of ECC was 68.3 (95% CI: 66.5-70.1), the mean dmft was 4.36 (95% CI: 4.17-4.55), and the filled rate was 1.2%. In multivariable modeling, associated factors for both prevalence and mean dmft were older age, rural areas, consumption of sweets before sleep, dental visit history, low household income, and low parental education level. Initiating toothbrushing after 3 years of age and being exclusively/ predominantly breastfed indicated only the prevalence; being female and frequently consuming sweetened milk/powdered milk indicated only the mean dmft.Conclusions: The preschool children in Guangdong Province experienced a significant amount of ECC. More attention should be given to children’s dental health for deciduous teeth. Associated factors for ECC included demographics, oral health measures, dietary factors, and socioeconomic factors.


Author(s):  
Udon Pongkawong ◽  
◽  
Jatupol Kampuansai ◽  
Rossarin Pollawatn ◽  
Arunothai Jampeetong ◽  
...  

Abstract “Dok Hin” is the Thai local name for Selaginella species that form rosettes. They commonly distributes in Siberia, Manchuria, southern China, Japan, the Philippines and Thailand. Morphology of Dok Hin is very resemble leading to misidentification. So, exactly number of species of Dok Hin in Thailand and their differences in morphological characteristics is not well understood. Thus, revision of morphological characters and phylogenetic confirmation of the taxonomic identification are needed. This study aims to examine morphological charateristics and phylogenetic patterns in eight populations of the Dok Hin in Northern Thailand. Morphology of Dok Hin from each populations was quantitatively examined using 15 vegetative and 6 reproductive characters meanwhile phylogenetic analyses was explored by DNA barcode ITS2. The results of the phylogenetic analysis revealed the existence of two species of Dok Hin, S. tamariscina and S. pulvinata. Selaginella tamariscina can be distinguished from S. pulvinata by its presence of a pseudotrunk above ground and ridges of dorsal leaves. On the other hand, the results of phylogenetic analysis indicated the differences among populations of S. pulvinata as well. Chiang Mai populations of S. pulvinata was characterized by peculiar set of characters long leaves and leaf apices look like caudate, while the rest of their populations have shorter leaves and leaf apices look like aristate. It indicates that S. pulvinata has genetic and phenotypic divergence among populations. However, additional studies of Dok Hin populations in other parts of Thailand and studies on different genetic markers are necessary to confirm the taxonomic status of S. pulvinata. Keywords: Dok Hin, Morphometric, Phylogeny, Pseudotrunk, Resurrection plant


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