scholarly journals The formation of perinucleolar bodies is important for normal leaf development and requires the zinc‐finger DNA‐binding motif in Arabidopsis ASYMMETRIC LEAVES2

2019 ◽  
Vol 101 (5) ◽  
pp. 1118-1134 ◽  
Author(s):  
Lilan Luo ◽  
Sayuri Ando ◽  
Yuki Sakamoto ◽  
Takanori Suzuki ◽  
Hiro Takahashi ◽  
...  
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Leaf Development ◽  
Binding Motif ◽  
Normal Leaf

scholarly journals Comparison of the aflR gene sequences of strains in Aspergillus section Flavi

Microbiology ◽  
2006 ◽  
Vol 152 (1) ◽  
pp. 161-170 ◽  
Author(s):  
Chao-Zong Lee ◽  
Guey-Yuh Liou ◽  
Gwo-Fang Yuan
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Aspergillus Parasiticus ◽  
Regulatory Gene ◽  
Aflatoxin Production ◽  
Agricultural Economics ◽  
Binding Motif ◽  
Fermented Foods ◽  
Pcr Products ◽  
Aspergillus Nomius

Aflatoxins are polyketide-derived secondary metabolites produced by Aspergillus parasiticus, Aspergillus flavus, Aspergillus nomius and a few other species. The toxic effects of aflatoxins have adverse consequences for human health and agricultural economics. The aflR gene, a regulatory gene for aflatoxin biosynthesis, encodes a protein containing a zinc-finger DNA-binding motif. Although Aspergillus oryzae and Aspergillus sojae, which are used in fermented foods and in ingredient manufacture, have no record of producing aflatoxin, they have been shown to possess an aflR gene. This study examined 34 strains of Aspergillus section Flavi. The aflR gene of 23 of these strains was successfully amplified and sequenced. No aflR PCR products were found in five A. sojae strains or six strains of A. oryzae. These PCR results suggested that the aflR gene is absent or significantly different in some A. sojae and A. oryzae strains. The sequenced aflR genes from the 23 positive strains had greater than 96·6 % similarity, which was particularly conserved in the zinc-finger DNA-binding domain. The aflR gene of A. sojae has two obvious characteristics: an extra CTCATG sequence fragment and a C to T transition that causes premature termination of AFLR protein synthesis. Differences between A. parasiticus/A. sojae and A. flavus/A. oryzae aflR genes were also identified. Some strains of A. flavus as well as A. flavus var. viridis, A. oryzae var. viridis and A. oryzae var. effuses have an A. oryzae-type aflR gene. For all strains with the A. oryzae-type aflR gene, there was no evidence of aflatoxin production. It is suggested that for safety reasons, the aflR gene could be examined to assess possible aflatoxin production by Aspergillus section Flavi strains.

scholarly journals The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor with a Novel Conserved Auxiliary DNA-Binding Motif†

Biochemistry ◽  
2008 ◽  
Vol 47 (44) ◽  
pp. 11415-11423 ◽  
Author(s):  
Ricardo Medina ◽  
Timothy Buck ◽  
Sayyed K. Zaidi ◽  
Angela Miele-Chamberland ◽  
Jane B. Lian ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Dna Binding ◽  
Zinc Finger ◽  
Histone Gene ◽  
Binding Motif ◽  
Zinc Finger Transcription Factor ◽  
Cell Cycle Regulator

An Accessory DNA Binding Motif in the Zinc Finger Protein Adr1 Assists Stable Binding to DNA and Can Be Replaced by a Third Finger†

Biochemistry ◽  
2000 ◽  
Vol 39 (3) ◽  
pp. 567-574 ◽  
Author(s):  
Elton T. Young ◽  
Nataly Kacherovsky ◽  
Cheng
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Binding Motif ◽  
Finger Protein

A model for the tertiary structure of the 28 residue DNA-binding motif (‘zinc finger’) common to many eukaryotic transcriptional regulatory proteins

1988 ◽  
Vol 2 (3) ◽  
pp. 209-218 ◽  
Author(s):  
Toby J. Gibson ◽  
Johan P.M. Postma ◽  
Raymond S. Brown ◽  
Patrick Argos
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Tertiary Structure ◽  
Regulatory Proteins ◽  
Binding Motif ◽  
Transcriptional Regulatory

scholarly journals GATA2 Zinc Finger Mutations Affect DNA-Binding and Promote Granulopoietic Differentiation

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 2779-2779
Author(s):  
Georg Leubolt ◽  
Belay Tizazu ◽  
Sayantanee Dutta ◽  
Monica Cusan ◽  
Theresa Sippenauer ◽  
...  
Keyword(s):  
Dna Binding ◽  
Board Of Directors ◽  
Zinc Finger ◽  
Transcriptional Activation ◽  
Research Funding ◽  
Luciferase Reporter ◽  
Binding Motif ◽  
Advisory Committees ◽  
Myeloid Malignancies ◽  
The Impact

Abstract GATA2 Zinc-Finger (ZF) mutations are associated with specific subgroups of myeloid malignancies. Alterations of the N-terminal ZF1 were identified in AML patients with biallelic CEBPA mutations, whereas the C-terminal ZF2 is typically affected by germline mutations, predisposing to MDS and AML, or by somatic lesions in CML blast crisis. Nevertheless, the context-dependent mechanism underlying GATA2 ZF2 mutations remains mostly unclear. Here, we set out to study the functional consequences of GATA2 ZF mutations in myeloid malignancies. In particular, we performed DNA-pulldown experiments, using FLAG-tagged full length GATA2 ZF WT and mutant proteins after expression in HEK293T cells and incubation of the cell lysates with biotinylated oligonucleotides encoding the binding-motif GATC. These experiments showed disruption of DNA binding for all GATA2 ZF mutants tested in vitro - regardless of the mutant positions within the ZF domains (Figure 1 A). Moreover, we studied the impact of GATA2 ZF mutations on the protein-interaction with Friend of GATA Protein 1 (FOG1; HGNC symbol ZFPM1). The influence of FOG1 on GATA2-dependent transcriptional activation was evaluated using a GATA-specific luciferase reporter. All GATA2 mutants tested were able to activate this reporter, although to variable extent. While co-expression of FOG1 overall counteracted GATA2-dependent transcriptional activation, this effect was significantly reduced for the GATA2 mutants L321F (located in ZF1) and T354M (located in ZF2). This suggested that both ZF domains are involved in the FOG1-interaction. To gain insights into the influence of GATA2 ZF1 mutation on hematopoiesis we performed colony forming cell (CFC)-assays. Lin- primary murine bone marrow cells expressing GATA2 WT or mutants were plated in methylcellulose supplemented with cytokines. Consistent with previous reports, GATA2 WT expression led to a reduced colony number, while this effect was decreased for both mutants A318T (ZF1) and L359V (ZF2). In particular, a higher number of CFU-G colonies were observed for the GATA2 mutant-expressing cells indicating a lineage shift towards granulopoiesis (Figure 1 B). In summary, we have shown that GATA2 mutations influence DNA-binding, protein-interactions and myeloid differentiation. Our findings further suggest that GATA2 ZF1 mutations may contribute to myeloid leukemogenesis through increased proliferation of granulocytic progenitors. Understanding the oncogenic collaboration of GATA2 mutations with other driver genes in distinct patient subgroups is a challenge ahead. Disclosures Hiddemann: Celgene: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; F. Hoffman-La Roche: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Janssen: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Bayer: Consultancy, Research Funding.

scholarly journals In vitro identification of DNA-binding motif for the new zinc finger protein AtYY1

2012 ◽  
Vol 44 (6) ◽  
pp. 483-489 ◽  
Author(s):  
Xueping Wu ◽  
Yongsheng Cheng ◽  
Tian Li ◽  
Zhao Wang ◽  
Jin-Yuan Liu
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Binding Motif ◽  
Finger Protein

scholarly journals Genome-Wide Binding Analyses of HOXB1 Revealed a Novel DNA Binding Motif Associated with Gene Repression

2021 ◽  
Vol 9 (1) ◽  
pp. 6
Author(s):  
Narendra Pratap Singh ◽  
Bony De Kumar ◽  
Ariel Paulson ◽  
Mark E. Parrish ◽  
Carrie Scott ◽  
...  
Keyword(s):  
Dna Binding ◽  
Target Genes ◽  
Embryonic Stem ◽  
Binding Motif ◽  
Binding Assays ◽  
Histone Marks ◽  
Genome Wide ◽  
Hox Cofactors

Knowledge of the diverse DNA binding specificities of transcription factors is important for understanding their specific regulatory functions in animal development and evolution. We have examined the genome-wide binding properties of the mouse HOXB1 protein in embryonic stem cells differentiated into neural fates. Unexpectedly, only a small number of HOXB1 bound regions (7%) correlate with binding of the known HOX cofactors PBX and MEIS. In contrast, 22% of the HOXB1 binding peaks display co-occupancy with the transcriptional repressor REST. Analyses revealed that co-binding of HOXB1 with PBX correlates with active histone marks and high levels of expression, while co-occupancy with REST correlates with repressive histone marks and repression of the target genes. Analysis of HOXB1 bound regions uncovered enrichment of a novel 15 base pair HOXB1 binding motif HB1RE (HOXB1 response element). In vitro template binding assays showed that HOXB1, PBX1, and MEIS can bind to this motif. In vivo, this motif is sufficient for direct expression of a reporter gene and over-expression of HOXB1 selectively represses this activity. Our analyses suggest that HOXB1 has evolved an association with REST in gene regulation and the novel HB1RE motif contributes to HOXB1 function in part through a repressive role in gene expression.

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