Leaf Diseases Classification on Peanut Leaves Based on Texture and Colour Features

Peanuts are a food crop commodity that Indonesians widely consume as a vegetable fat and protein source. However, the quality and quantity of peanut productivity may decline, one of which is due to plant diseases. Efforts that can be made to maintain peanut productivity are the application of technology to detect peanut plant diseases early; thus, disease control can be carried out earlier. This study presents a technology development application, particularly digital image processing, to identify disease features of infected peanut leaves based on GLCM texture features and colour features in the HSV colour space and classified using the SVM method. The development of the SVM method that is applied is the Multiclass SVM with the DAGSVM strategy, which can classify more than two classes. Based on the experimental results, it confirms that the combination of HSV colour features and GLCM texture features with an angular orientation of 0 degrees and classified by the Multiclass SVM method with polynomial kernels produces the highest accuracy, i.e. 99.1667% for leaf spot class, 97.5% for leaf rust class, 98.8333% for eyespot class, 100% for normal leaf class and 100% for other leaf class.

FOLIAR BIOCHEMICAL CHANGES DURING GALL FORMATION IN MITRAGYNA PARVIFOLIA

Mitragyna parvifolia is a medicinally important plant which is native to India and Shrilanka. This plant is widely used by tribal people of Rajasthan and other Ayurvedic practitioners. Normal leaf and leaf gall of Mitragyna parvifolia were collected and categorized in normal (healthy leaf) and galled leaf. In the present investigation an attempt was made to study the changes of some metabolites during the gall formation on leaf of Mitragyna parvifolia attacked by gall insect. Total soluble sugar, total phenol and peroxidase activities were found high in gall leaves as compared to healthy leaves but chlorophyll contents and polyphenol oxidase activities were recorded high in normal leave and protein contents were slightly high in normal leaves as compared to infected or gall leaves.

Optimal Duration of Drought Stress Near Harvest for Promoting Bioactive Compounds and Antioxidant Capacity in Kale with or without UV-B Radiation in Plant Factories

Among abiotic stresses, both drought and UV-B radiation effectively trigger the accumulation of secondary metabolites, and can be widely applied in plant factories. The objectives of this study were to investigate antioxidant accumulation under drought stress alone, or in combination with UV-B radiation near harvest, and to determine an optimal treatment time for maximum antioxidant production. Kale (Brassica oleracea L. var. acephala) plants were grown in a plant factory and harvested at 42 days after transplanting. The single and combination treatments lasted for 7 to 1 days and 4 to 2 days before harvest, respectively. The results of both Fv/Fm (maximal photochemical efficiency in photosystem II) and leaf water potential could ensure the function of photosynthesis and maintain normal leaf moisture in single drought treatments of less than 4 days. The total phenolic and flavonoid contents and antioxidant activities were significantly increased in both single and combination treatments for 3 to 4 days, compared to other treatments. The supplementary UV-B treatments showed no extra formation of antioxidants compared to the single drought treatments. As a result, drought for 3 days before harvest could achieve the highest potential value of kale as a source of natural antioxidants.

Isolation, identification and quantification of gallic acid (gallotannins) through HPTLC in leaf galls of Madhuca longifolia (Koenig) j.f. Macb

Plant galls (cecidia) are pathologically produced cells, tissues, or organs of plants that have developed by hypertrophy and hyperplasia of plant tissues under the effect of gall causing organisms. Madhuca longifolia (Koenig) j.f Macb. is an economically and medicinally very important plant of the family Sapotaceae. It is a tropical mixed deciduous tree. Various galls due to insect infestation are found on almost all tree species. Leaf galls of Madhuca longifolia induced by insects of order Diptera and Hymenoptera represent a unique pattern of chemical perturbations which normally do not occur in normal leaf. During the present investigation, an accurate, fast and easy HPTLC method was followed for quantification of gallic acid occur in the normal leaf and dried leaf galls of the Madhuca longifolia plant. The protocol followed in this study resulted in an intense peak and was able to give a good resolution of gallic acid from normal leaf and galled tissues of Madhuca longifolia (Koenig) j.f. Macb. Adaxial leaf gall induced by insect Mohwadiplosis Orientalis Rao belonging to Diptera class, Marginal gall induced by another Dipteran and vein gall by chalcidoiid Hymenopteran class of insect. Variation in Gallic acid present in normal leaf and leaf galls of Madhuca longifolia was critically reported. It was found that Gallic acid content increased almost two folds in gall tissues as compared to normal leaf tissue. Galled leaf (Dipteran adaxial gall) had a maximum amount of Gallic acid (344.4 ng) while in normal leaf and another leaf galls had less amount of gallic acid was found (180ng). This study is of practical importance because gallic acid is the most important active phenolic acid, which may be correlated with post-infection biochemical defense. Compound gallic acid has been reported for the first time from leaf galls of Madhuca longifolia (Koenig) j. f. Macb. Plant in the study.

Inheritance of a Novel Heterozygous Peanut Mutant, 5-Small Leaflet

ABSTRACT An unusual 5-Small Leaflet mutant plant was found within the ‘Georgia Green' runner-type peanut (Arachis hypogaea L.) cultivar. Subsequent selfing has not established a true-breeding 5-Small Leaflet genotype. It continues to segregate normal and 5-Small Leaflet plants but with a reduced number of normal leaf plants upon selection for 5-Small Leaflet phenotypes after several self-generations. F1, F2, F3, and F4 data suggests that the 5-Small Leaflet trait is dominant or possibly pseudo-dominant. Likewise, the 5-Small Leaflet mutant can only be used as a pollen parent in crosses, and it has approximately a 1:1 ratio of elongated to normal stigmas, respectively, on individual plants. This is an example of a novel heterozygous peanut mutant plant found within the cultivated allotetraploid peanut.

Handheld Device Based on Image Processing Technique for Detecting Multiple Diseases of Apple Leaves

The present invention discloses a handheld device for multiple disease detection from apple leaf and method thereof. An algorithm is developed in combination with image processing and gray level co occurrence matrix for the classification of normal leaf and diseased apple leaf. The device performs image processing by segmentation of the image and then by using extracted features. The classification and detection of these diseases impart an early solution to the farmers leading less harm to the apple crops. Conventional Detection of the diseases through naked eye can sometimes be faulty. Therefore the device of present invention helps farmers to detect the accurate diseases and provide timely solutions for the same. The present invention increases the throughput and reduces subjectiveness of the previously used conventional methods by proving early and precise disease detection from apple leaves.

Revolute-Leaf, a New Completely Dominant Mutant in Peanut

ABSTRACT A Revolute-Leaf mutant plant was discovered in an advanced Georgia peanut (Arachis hypogaea L.) breeding line, GA 112702. The mutant had leaf margins that curve downward on each of the four small light-green leaflets. Two cross combinations were used to determine the inheritance of this new mutant. F1, F2, and F3 segregation data strongly supported a single completely dominant gene, designated Rev, controlling the inheritance of the Revolute-Leaf mutant. The F2:3 homozygous revolute-leaf individual plants had shorter mainstem heights, narrower leaflet length and width, narrower canopy width, reduced pod and seed weights, but similar SMK percentages compared to the F2:3 homozygous normal leaf plants resulting from the same closely related cross combination (GA 112702 x Revoluted-Leaf mutant).