Akt activation‐dependent protective effect of wild ginseng adventitious root protein against UVA ‐induced NIH‐3T3 cell damage

Nowadays, because of the industrialization, a lot of contaminant were available ; the consequences of this availability are apparition of diseases including neurodegeneration. Neurodegenerative diseases of the human brain comprise a variety of disorders that affect an increasing percentage of the population. This study is based on the effect of the Boswellic resin, which is from a medicinal plant and known for its antioxidant effects on nerve cell damage. The objective of this work was to evaluate the in vitro and in vivo effects of the Boswellic resin on anticholinesterase activity and Alzheimer’s disease (AD) induced by D-galactose and aluminum tetrachloride in Swiss mice. Chemical composition of the resin essential oil was identified by the CG-MS analysis. The antioxidant activity was also assessed by the DMPD and metal chelation methods. In order to understand the mechanism of memory improvement, the acetylcholinesterase, AChE, and butyrylcholinesterase, BChE, inhibitory assays were performed. In vivo part of the study was achieved on Swiss mice divided into four groups: control, AD model, treated AD, and treated control group. The identification of chemical composition by CG-MS reach the 89.67% of the total extract compounds presented some very important molecules (p-Cymene, n-Octyl acetate, α-Pinene…). The present study proves that Boswellic resin improves memory and learning in treated Alzheimer’s group, modulates the oxidative stress and be involved in the protective effect against amyloid deposition and neurodegeneration, and stimulates the immune system in mice’s brain.

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The potent protective effect of wild ginseng (Panax ginseng C.A. Meyer) against benzo[α]pyrene-induced toxicity through metabolic regulation of CYP1A1 and GSTs

Journal of Ethnopharmacology ◽

10.1016/j.jep.2007.05.014 ◽

2007 ◽

Vol 112 (3) ◽

pp. 568-576 ◽

Cited By ~ 47

Author(s):

Sang Il Gum ◽

Sung Jun Jo ◽

Sang Hyun Ahn ◽

Sang Geon Kim ◽

Jin-Taek Kim ◽

...

Keyword(s):

Protective Effect ◽

Panax Ginseng ◽

Metabolic Regulation ◽

Wild Ginseng

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Protective effect of triterpenes against diabetes-induced β-cell damage: An overview of in vitro and in vivo studies

Pharmacological Research ◽

10.1016/j.phrs.2018.10.004 ◽

2018 ◽

Vol 137 ◽

pp. 179-192 ◽

Cited By ~ 8

Author(s):

Sihle E. Mabhida ◽

Phiwayinkosi V. Dludla ◽

Rabia Johnson ◽

Musawenkosi Ndlovu ◽

Johan Louw ◽

...

Keyword(s):

Protective Effect ◽

Cell Damage ◽

In Vivo Studies ◽

Β Cell

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The Cytoprotective and Cell Recovery Properties of Apple Extracts on H2O2 induced-NIH3T3 Cells: An Anti Aging Candidate

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev9iss2pp78-85 ◽

2018 ◽

Vol 9 (2) ◽

pp. 78 ◽

Cited By ~ 1

Author(s):

Nunuk Aries Nurulita ◽

Anjar Mahardian Kusuma ◽

Darsini Darsini ◽

Weny Delvia ◽

Veby Tri Yulianti

Keyword(s):

Free Radicals ◽

Vitamin C ◽

Protective Effect ◽

Cell Damage ◽

Cell Aging ◽

Fibroblast Cells ◽

Cell Recovery ◽

High Concentration ◽

High Potency ◽

Nih3t3 Cells

Apple contains high concentration of phenolic compounds that protect cells from oxidative stress. The prolong exposure of free radicals may induce cell damage and premature cell aging. Both local and imported apple contain flavonoid, saponin, tannin, steroid, and terpenoid. The extract of local and imported apples showed low toxicity on NIH3T3 fibroblast cells, with IC50 value of 529 and 463 µg/mL, respectively. Both apple extracts (50 – 250 µg /mL) protected three-day-H2O2 induced-cell damage and cell death. Protective effect was observed as the viability increase of treated cells compared to untreated ones. The protective effect of both extracts were higher than the effect of vitamin C as standard antioxidant at this study. Both apple extracts could reverse cell damage caused by three-hour-high concentration H2O2 exposure, similar with vitamin C. Low concentration of both extracts (50 µg /mL) induced the increase of fibroblast cells’ proliferation kinetics. The extract of imported apple showed higher properties of protective, cell recovery and proliferation of fibroblast cells tha local apple, but not statistically significance. This study concludes that the extract of local and imported apples have high potency in cytoprotective effect and cell recovery of damaged cells caused by free radicals induction. Both apple extracts have high potency to be developed the candidate of antiaging and cells’ regeneration agent.Key words: antiaging, cell recovery, cytoprotective, NIH3T3 cells

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Calcium-dependent opening of a non-specific pore in the mitochondrial inner membrane is inhibited at pH values below 7. Implications for the protective effect of low pH against chemical and hypoxic cell damage

Biochemical Journal ◽

10.1042/bj2780715 ◽

1991 ◽

Vol 278 (3) ◽

pp. 715-719 ◽

Cited By ~ 104

Author(s):

A P Halestrap

Keyword(s):

Protective Effect ◽

Adenine Nucleotide ◽

Cell Damage ◽

Liver Mitochondria ◽

Low Ph ◽

Hypoxic Cell ◽

Inner Membrane ◽

Calcium Dependent ◽

Mitochondrial Inner Membrane ◽

The Matrix

1. The rate of opening of the Ca(2+)-induced non-specific, cyclosporin A-inhibited, pore of the mitochondrial inner membrane of rat heart and liver mitochondria at pH 6.0 was less than 10% of that at pH 7.4. 2. The effect could not be explained by inhibition of Ca2+ uptake into the mitochondria, or of the matrix peptidyl-prolyl cis-trans isomerase (PPIase), or of the Ca(2+)-induced conformational change of the adenine nucleotide translocase. 3. It is suggested that the proposed interaction of matrix PPIase with the ‘c’ conformation of the adenine nucleotide carrier in the presence of Ca2+ [Griffiths & Halestrap (1991) Biochem. J. 274, 611-614] is inhibited by low pH. 4. The relevance of this to the protective effect of low pH on hypoxic and chemical-induced cell damage is discussed.

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l-carnitine protects human hepatocytes from oxidative stress-induced toxicity through Akt-mediated activation of Nrf2 signaling pathway

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2015-0305 ◽

2016 ◽

Vol 94 (5) ◽

pp. 517-525 ◽

Cited By ~ 9

Author(s):

Jinlian Li ◽

Yanli Zhang ◽

Haiyun Luan ◽

Xuehong Chen ◽

Yantao Han ◽

...

Keyword(s):

Protective Effect ◽

Signaling Pathway ◽

Nuclear Translocation ◽

Cell Damage ◽

Binding Activity ◽

Akt Pathway ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Akt Inhibitor ◽

Nrf2 Signaling Pathway

In our previous study, l-carnitine was shown to have cytoprotective effect against hydrogen peroxide (H2O2)-induced injury in human normal HL7702 hepatocytes. The aim of this study was to investigate whether the protective effect of l-carnitine was associated with the nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) pathway. Our results showed that pretreatment with l-carnitine augmented Nrf2 nuclear translocation, DNA binding activity and heme oxygenase-1 (HO-1) expression in H2O2-treated HL7702 cells, although l-carnitine treatment alone had no effect on them. Analysis using Nrf2 siRNA demonstrated that Nrf2 activation was involved in l-carnitine-induced HO-1 expression. In addition, l-carnitine-mediated protection against H2O2 toxicity was abrogated by Nrf2 siRNA, indicating the important role of Nrf2 in l-carnitine-induced cytoprotection. Further experiments revealed that l-carnitine pretreatment enhanced the phosphorylation of Akt in H2O2-treated cells. Blocking Akt pathway with inhibitor partly abrogated the protective effect of l-carnitine. Moreover, our finding demonstrated that the induction of Nrf2 translocation and HO-1 expression by l-carnitine directly correlated with the Akt pathway because Akt inhibitor showed inhibitory effects on the Nrf2 translocation and HO-1 expression. Altogether, these results demonstrate that l-carnitine protects HL7702 cells against H2O2-induced cell damage through Akt-mediated activation of Nrf2 signaling pathway.

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Protective Effect of Astaxanthin on Blue Light Light-Emitting Diode-Induced Retinal Cell Damage via Free Radical Scavenging and Activation of PI3K/Akt/Nrf2 Pathway in 661W Cell Model

Marine Drugs ◽

10.3390/md18080387 ◽

2020 ◽

Vol 18 (8) ◽

pp. 387 ◽

Cited By ~ 1

Author(s):

Chao-Wen Lin ◽

Chung-May Yang ◽

Chang-Hao Yang

Keyword(s):

Antioxidant Enzymes ◽

Free Radical ◽

Protective Effect ◽

Blue Light ◽

Nuclear Translocation ◽

Light Exposure ◽

Cell Damage ◽

Radical Scavenging ◽

Photoreceptor Cells ◽

Light Emitting

Light-emitting diodes (LEDs) are widely used and energy-efficient light sources in modern life that emit higher levels of short-wavelength blue light. Excessive blue light exposure may damage the photoreceptor cells in our eyes. Astaxanthin, a xanthophyll that is abundantly available in seafood, is a potent free radical scavenger and anti-inflammatory agent. We used a 661W photoreceptor cell line to investigate the protective effect of astaxanthin on blue light LED-induced retinal injury. The cells were treated with various concentrations of astaxanthin and then exposed to blue light LED. Our results showed that pretreatment with astaxanthin inhibited blue light LED-induced cell apoptosis and prevented cell death. Moreover, the protective effect was concentration dependent. Astaxanthin suppressed the production of reactive oxygen species and oxidative stress biomarkers and diminished mitochondrial damage induced by blue light exposure. Western blot analysis confirmed that astaxanthin activated the PI3K/Akt pathway, induced the nuclear translocation of Nrf2, and increased the expression of phase II antioxidant enzymes. The expression of antioxidant enzymes and the suppression of apoptosis-related proteins eventually protected the 661W cells against blue light LED-induced cell damage. Thus, our results demonstrated that astaxanthin exerted a dose-dependent protective effect on photoreceptor cells against damage mediated by blue light LED exposure.

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Antioxidant Effects and Cytoprotective Potentials of Herbal Tea against H2O2-Induced Oxidative Damage by Activating Heme Oxygenase1 Pathway

BioMed Research International ◽

10.1155/2020/7187946 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Ke-Xin Zhang ◽

Jian-Bin Tan ◽

Cheng-Liang Xie ◽

Rong-Bo Zheng ◽

Xiao-Dan Huang ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Effect ◽

Functional Foods ◽

Cell Damage ◽

Damage Model ◽

Enrichment Analysis ◽

Protective Effects ◽

Herbal Tea ◽

Antioxidant Effects

Herbal tea with antioxidant ingredients has gained increasing attention in the field of functional foods due to their amelioration potential in aging-related diseases. Wanglaoji herbal tea (WHT) is a kind of traditional beverage made from herbal materials. This study was performed to investigate its antioxidant activity and identify its protective effect on a H2O2-induced cell damage model. In this study, we identified six kinds of phenolic acids with antioxidant activity in WHT, among which rosmarinic acid had the highest content and the highest contribution ratio to the antioxidant activity of WHT. Moreover, compared with the H2O2-induced damage group, the WHT treatment group can significantly increase the viability of cells and decrease the ratio of senescence-associated β-galactosidase-positive cells, intracellular malondialdehyde levels, and the percentage of G1 phase. Furthermore, enrichment analysis of differentially expressed genes revealed that heme oxygenase1 (HMOX1) was a key gene for protective effect of WHT on oxidative stress-induced cell damage. Thus, WHT exerted protective effects not only by scavenging reactive oxygen species but also by inducing the expression of cytoprotective genes by activating the HMOX1 pathway, which showed that WHT had a potential of promoting health by reducing oxidative stress-induced cell damage.

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