scholarly journals CO-DIGESTION OF GREASY WASTE AND MBR SLUDGE USING BIO-AUGMENTED PSEUDOMONAS PUTIDA: EFFECT OF BUFFERING AGENTS

2021 ◽  
Vol 11 (4) ◽  
pp. 1-15
Author(s):  
Vongsadet Phommachanh ◽  
Wilai Chiemchaisri ◽  
Chart Chiemchaisri

This study aims to investigate the bio-augmentation of Pseudomonas putida for initializing fat, oil,and grease (FOG) biodegradation in a co-digestion of the greasy waste and the membranebioreactor (MBR) excess sludge. The MBR sludge could be an additional nitrogen sourceconcurrently as a waste matrix fluidizer. Three rounds of a fed-batch of an HRT of 56-71 dayswere carried out. The first (B1) and second (B2) batches using phosphate (Na2HPO4) solution as abuffering system. It showed that the digesters with bio-augmented P. putida could remove moreFOG than that of the control. In the third batch (B3), the buffering solution was changed tobicarbonate (NaHCO3) with the interval bio-augmentation of P. putida in different doses; the 250ml (B3, T-250) and the 1000 ml (B3, T-1000) of P. putida culture every two weeks dosing. Thehighest FOG removal was found in both conditions with the removal efficiency of 79.77%. Thisstudy suggests that bicarbonate was a suitable buffer in the co-digestion process for the intervalbio-augmentation of P. putida. Moreover, it showed that a higher interval dose (B3, T-1000) of P.putida gave a more methane production rate of 981.08 ml/g TVS. d compared to the lower dose ofB3(T-250) with 353.75 ml/g TVS. d.

2014 ◽  
Vol 69 (9) ◽  
pp. 1911-1918 ◽  
Author(s):  
R. Cano ◽  
A. Nielfa ◽  
A. Pérez ◽  
L. Bouchy ◽  
M. Fdz-Polanco

Grease waste (GW) is an adequate substrate for sewage sludge co-digestion since, coming from a waste water treatment plant, it has a high methane potential (489 NmLCH4/gVSin); however, no synergistic effect takes place when co-digesting with 52%VS grease. Conversely, thermal hydrolysis (TH) improves the anaerobic digestion of GW (43% higher kinetics) and biological sludge (29% more methane potential). Therefore, the application of TH to a co-digestion process was further studied. First, biochemical methane potential tests showed that the best configuration to implement the TH to the co-digestion process is pretreating the biological sludge alone, providing a 7.5% higher methane production (398 NmLCH4/gVSin), 20% faster kinetics and no lag-phase. Its implementation in a fed-batch operation resulted in considerable methane production (363 NmLCH4/gVSin) and TH improved the rheology and dewaterability properties of the digestate. This leads to important economical savings when combined with co-digestion, reducing final waste management costs and showing interesting potential for full-scale application.


Author(s):  
Tolulope Adeleye ◽  
Hyeongu Yeo ◽  
Hisham Hafez ◽  
Rajesh Seth ◽  
Nihar Biswas

The potential improvement in methane recovery and reaction kinetics from different mixes of potato peel (PP) and pig manure (PM) in a single stage anaerobic co-digestion/mono-digestion process was investigated in a laboratory study. The highest methane yield of 231 mL/g TCODadded was observed in the 50:50 mix of potato peel and pig manure. Compared to the mono-digested substrates, co-digestion of PP and PM at 75:25, 50:50 and 25:75 synergistically improved methane yield by 17%, 25% and 11%, respectively. The co-digested mixes also produced methane at a faster rate, with the fastest methane production rate occurring at the 50:50 mix. Thus, co-digestion of potato peel and pig manure enhanced the methane yield and reaction kinetics. Hence, co-digestion rather than mono-digestion should be actively considered when a carbon rich waste (such as potato peel) and nutrient rich waste (such as pig manure) are available within reasonable proximity.


2012 ◽  
Vol 118 (3) ◽  
pp. 210-212 ◽  
Author(s):  
Manju Puri ◽  
Poonam Taneja ◽  
Neha Gami ◽  
Harmeet S. Rehan

Author(s):  
Miguel Casallas-Ojeda ◽  
Sully Meneses-Bejarano ◽  
Ronald Urueña-Argote ◽  
Luis Fernando Marmolejo-Rebellón ◽  
Patricia Torres-Lozada

1997 ◽  
Vol 35 (8) ◽  
pp. 209-215 ◽  
Author(s):  
Shuzo Tanaka ◽  
Toshio Kobayashi ◽  
Ken-ichi Kamiyama ◽  
Ma. Lolita N. Signey Bildan

Effects of pretreatment on the anaerobic digestion of waste activated sludge (WAS) were investigated in terms of VSS solubilization and methane production by batch experiments. The methods of pretreatment studied are NaOH addition (chemical), heating (thermal) and heating with NaOH addition (thermochemical) to the domestic WAS and to the combined WAS from domestic, commercial and industrial wastewaters. The thermochemical pretreatment gave the best result among three methods in the combined WAS, i.e., the VSS was solubilized by 40-50% and the methane production increased by more than 200% over the control when the WAS was heated at 130°C for 5 minutes with the dose 0.3 g NaOH/g VSS. In the domestic WAS, the VSS solubilization rate was 70-80% but the increase of the methane production was about 30% after thermochemically pretreated. The domestic WAS consists of 41% protein, 25% lipid and 14% carbohydrate on COD basis, and the solubilization rate of protein, which is the largest constituent of the WAS, was 63% in the thermochemical pretreatment. Although the effect of the thermochemical pretreatment on the methane production was higher to the combined WAS than to the domestic WAS, the methane production rate was 21.9 ml CH4/g VSSWAS·day in the domestic WAS and 12.8 ml CH4/g VSSWAS·day in the combined WAS.


2019 ◽  
Vol 273 ◽  
pp. 425-430 ◽  
Author(s):  
Hairong Yuan ◽  
Ruolin Guan ◽  
Akiber Chufo Wachemo ◽  
Chao Zhu ◽  
Dexun Zou ◽  
...  

2000 ◽  
Vol 42 (10-11) ◽  
pp. 247-255 ◽  
Author(s):  
J. Paing ◽  
B. Picot ◽  
J. P. Sambuco ◽  
A. Rambaud

Sludge accumulation and the characteristics of anaerobic digestion in sludge had been investigated in a primary anaerobic lagoon. Methanogenic potential of sludge was evaluated by an anaerobic digestion test which measured the methane production rate. Sludge was sampled at several points in the lagoon to determine spatial variations and with a monthly frequency from the start-up of the lagoon to observe the development of anaerobic degradation. Maximum amounts of sludge accumulated near the inlet. The mean methane production of sludge was 2.9 ml gVS–1 d–1. Sludge near the outlet presented a greater methanogenic activity and a lesser concentration of volatile fatty acids than near the inlet. The different stages of anaerobic degradation were spatially separated, acidogenesis near the inlet and methanogenesis near the outlet. This staged distribution seemed to increase efficiency of anaerobic fermentation compared with septic tanks. Methane release at the surface of the lagoon was estimated to be very heterogeneous with a mean of 25 l m–2 d–1. The development of performance and sludge characteristics showed the rapid beginning of methanogenesis, three months after the start-up of the anaerobic lagoon. Considering the volume of accumulated sludge, it could however be expected that methanogenic activity would further increase.


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