Nanotopographic Biomaterials for Isolation of Circulating Tumor Cells

2014 ◽  
Vol 5 (4) ◽  
Author(s):  
Weiyi Qian ◽  
Yan Zhang ◽  
Andrew Gordon ◽  
Weiqiang Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Cancer Metastasis ◽  
Early Cancer ◽  
Tumor Mass ◽  
Early Cancer Diagnosis ◽  
Capture And Release ◽  
Increasing Demand ◽  
Detection Technologies

Circulating tumor cells (CTCs) shed from the primary tumor mass and circulating in the bloodstream of patients are believed to be vital to understand of cancer metastasis and progression. Capture and release of CTCs for further enumeration and molecular characterization holds the key for early cancer diagnosis, prognosis and therapy evaluation. However, detection of CTCs is challenging due to their rarity, heterogeneity and the increasing demand of viable CTCs for downstream biological analysis. Nanotopographic biomaterial-based microfluidic systems are emerging as promising tools for CTC capture with improved capture efficiency, purity, throughput and retrieval of viable CTCs. This review offers a brief overview of the recent advances in this field, including CTC detection technologies based on nanotopographic biomaterials and relevant nanofabrication methods. Additionally, the possible intracellular mechanisms of the intrinsic nanotopography sensitive responses that lead to the enhanced CTC capture are explored.

scholarly journals Current detection technologies for circulating tumor cells

2017 ◽  
Vol 46 (8) ◽  
pp. 2038-2056 ◽  
Author(s):  
Zheyu Shen ◽  
Aiguo Wu ◽  
Xiaoyuan Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Early Cancer ◽  
Cancer Recurrence ◽  
Early Cancer Diagnosis ◽  
Chemotherapeutic Efficacy ◽  
Anti Cancer ◽  
Current Detection ◽  
Detection Technologies

CTC detection can be used for early cancer diagnosis, earlier evaluation of cancer recurrence and chemotherapeutic efficacy, and choice of individual sensitive anti-cancer drugs.

Engineered red blood cells for capturing circulating tumor cells with high performance

Nanoscale ◽  
2018 ◽  
Vol 10 (13) ◽  
pp. 6014-6023 ◽  
Author(s):  
Dao-Ming Zhu ◽  
Lei Wu ◽  
Meng Suo ◽  
Song Gao ◽  
Wei Xie ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
High Performance ◽  
Early Cancer ◽  
Treatment Monitoring ◽  
Prognostic Evaluation ◽  
Early Cancer Diagnosis

Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation.

scholarly journals Nanotechnology-Based Strategies for Early Cancer Diagnosis Using Circulating Tumor Cells as a Liquid Biopsy

2018 ◽  
Vol 2 (1) ◽  
pp. 21-41 ◽  
Author(s):  
Qinqin Huang ◽  
Yin Wang ◽  
Xingxiang Chen ◽  
Yimeng Wang ◽  
Zhiqiang Li ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Liquid Biopsy ◽  
Early Cancer ◽  
Early Cancer Diagnosis

High Sensitivity Detection of Tumor Cells in Biological Samples using Multivalent Aptamer Strand Displacement Strategy

The Analyst ◽  
2022 ◽  
Author(s):  
Jieru Xu ◽  
Jiahui Xiang ◽  
Jialing Chen ◽  
Tao Wan ◽  
Hongli Deng ◽  
...  
Keyword(s):  
Cell Surface ◽  
Tumor Cells ◽  
Cancer Diagnosis ◽  
Biological Samples ◽  
Cell Membranes ◽  
Early Cancer ◽  
High Sensitivity ◽  
Strand Displacement ◽  
Early Cancer Diagnosis ◽  
High Sensitivity Detection

Monitoring the cell surface-expressed nucleolin facilitates early cancer diagnosis. Herein, we developed multivalent aptamer displacement strand duplex strategy on the cell membranes utilizing a multi-receptor co-recognition design for improving sensitivity...

scholarly journals FlowCell-enriched circulating tumor cells as a predictor of lung cancer metastasis

Human Cell ◽  
2021 ◽  
Author(s):  
Yan Lu ◽  
Yushuang Zheng ◽  
Yuhong Wang ◽  
Dongmei Gu ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metastasis ◽  
Lung Tumor ◽  
Early Stage ◽  
High Rate ◽  
Early Stage Lung Cancer ◽  
Lung Cancer Metastasis ◽  
Number Of Patients

AbstractLung cancer is the most fetal malignancy due to the high rate of metastasis and recurrence after treatment. A considerable number of patients with early-stage lung cancer relapse due to overlooked distant metastasis. Circulating tumor cells (CTCs) are tumor cells in blood circulation that originated from primary or metastatic sites, and it has been shown that CTCs are critical for metastasis and prognosis in various type of cancers. Here, we employed novel method to capture, isolate and classify CTC with FlowCell system and analyzed the CTCs from a cohort of 302 individuals. Our results illustrated that FlowCell-enriched CTCs effectively differentiated benign and malignant lung tumor and the total CTC counts increased as the tumor developed. More importantly, we showed that CTCs displayed superior sensitivity and specificity to predict lung cancer metastasis in comparison to conventional circulating biomarkers. Taken together, our data suggested CTCs can be used to assist the diagnosis of lung cancer as well as predict lung cancer metastasis. These findings provide an alternative means to screen early-stage metastasis.

Biomimetic recognition strategy for efficient capture and release of circulating tumor cells

2021 ◽  
Vol 188 (6) ◽  
Author(s):  
Ji Zheng ◽  
Dayong Li ◽  
Jin Jiao ◽  
Chengjie Duan ◽  
Youjing Gong ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Capture And Release

scholarly journals Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2723
Author(s):  
Yu-Ping Yang ◽  
Teresa M. Giret ◽  
Richard J. Cote
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Ex Vivo ◽  
Detection Sensitivity ◽  
Clinical Utilization ◽  
Diagnosis And Prognosis ◽  
Early Cancer Diagnosis ◽  
Potential Marker ◽  
Potential Applications ◽  
Downstream Analysis

Circulating tumor cells (CTCs) have been recognized as a major contributor to distant metastasis. Their unique role as metastatic seeds renders them a potential marker in the circulation for early cancer diagnosis and prognosis as well as monitoring of therapeutic response. In the past decade, researchers mainly focused on the development of isolation techniques for improving the recovery rate and purity of CTCs. These developed techniques have significantly increased the detection sensitivity and enumeration accuracy of CTCs. Currently, significant efforts have been made toward comprehensive molecular characterization, ex vivo expansion of CTCs, and understanding the interactions between CTCs and their associated cells (e.g., immune cells and stromal cells) in the circulation. In this review, we briefly summarize existing CTC isolation technologies and specifically focus on advances in downstream analysis of CTCs and their potential applications in precision medicine. We also discuss the current challenges and future opportunities in their clinical utilization.

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