Production of Fluoropolymer Microchips for Droplet Microfluidics and DNA Amplification

2010 ◽  
Author(s):  
Stefano Begolo ◽  
Guillaume Colas ◽  
Laurent Malaquin ◽  
Jean-Louis Viovy
Keyword(s):  
Low Temperatures ◽  
Dna Amplification ◽  
Droplet Microfluidics ◽  
Cross Contamination ◽  
Uniform Pressure ◽  
Microfluidic Chips ◽  
Fabrication Technique ◽  
Flexible Membrane ◽  
Low Surface Energy ◽  
Solvent Bonding

In this paper we present a novel fabrication technique for production of monolithic microfluidic chips made from a fluoropolymer (Dyneon THV). This material retains numerous properties of commonly used fluoropolymers (low surface energy and compatibility with chemicals such as organic solvents or fluorinated oil), and is easily processable at relatively low temperatures (lower than 180°C). We used hot embossing to mold microstructures on flat sheets of this polymer. The microchips are sealed through a combination of thermal and solvent bonding by applying uniform pressure with a flexible membrane. These closed channels can be used for the production and circulation of aqueous droplets in fluorinated oil. This droplet microfluidic configuration is suitable for DNA amplification since it avoids cross contamination between adjacent droplets.

scholarly journals Low Distortion Solvent Bonding of Microfluidic Chips

2016 ◽  
Vol 141 ◽  
pp. 130-137 ◽  
Author(s):  
Shu Pei Ng ◽  
Florencia Edith Wiria ◽  
Nam Beng Tay
Keyword(s):  
Microfluidic Chips ◽  
Low Distortion ◽  
Solvent Bonding

scholarly journals Microfabrication of Nonplanar Polymeric Microfluidics

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 491
Author(s):  
Pin-Chuan Chen ◽  
Chung-Ying Lee ◽  
Lynh Duong
Keyword(s):  
Dimensional Space ◽  
Three Dimensional ◽  
Microlens Array ◽  
Microfluidic Chips ◽  
Pmma Substrate ◽  
In Vitro Characterization ◽  
First Case ◽  
Definition Of ◽  
Solvent Bonding

For four decades, microfluidics technology has been used in exciting, state-of-the-art applications. This paper reports on a novel fabrication approach in which micromachining is used to create nonplanar, three-dimensional microfluidic chips for experiments. Several parameters of micromachining were examined to enhance the smoothness and definition of surface contours in the nonplanar poly(methyl methacrylate) (PMMA) mold inserts. A nonplanar PMMA/PMMA chip and a nonplanar polydimethylsiloxane (PDMS)/PMMA chip were fabricated to demonstrate the efficacy of the proposed approach. In the first case, a S-shape microchannel was fabricated on the nonplanar PMMA substrate and sealed with another nonplanar PMMA via solvent bonding. In the second case, a PDMS membrane was casted from two nonplanar PMMA substrates and bonded on hemispherical PMMA substrate via solvent bonding for use as a microlens array (MLAs). These examples demonstrate the effectiveness of micromachining in the fabrication of nonplanar microfluidic chips directly on a polymeric substrate, as well as in the manufacture of nonplanar mold inserts for use in creating PDMS/PMMA microfluidic chips. This technique facilitates the creation of nonplanar microfluidic chips for applications requiring a three-dimensional space for in vitro characterization.

scholarly journals In Situ Fine‐Tuning of Microfluidic Chips by Swelling and Its Application to Droplet Microfluidics

2019 ◽  
Vol 4 (8) ◽  
pp. 1900232
Author(s):  
Juan H. González‐Estefan ◽  
Mathieu Gonidec ◽  
Thi Thiet Vu ◽  
Nathalie Daro ◽  
Guillaume Chastanet
Keyword(s):  
Droplet Microfluidics ◽  
Fine Tuning ◽  
Microfluidic Chips

scholarly journals Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Patrick Hardinge ◽  
Divesh K. Baxani ◽  
Thomas McCloy ◽  
James A. H. Murray ◽  
Oliver K. Castell
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Soft Matter ◽  
Low Cost ◽  
Diagnostic Technique ◽  
Dna Amplification ◽  
Droplet Microfluidics ◽  
Nucleic Acid Amplification ◽  
Molecular Diagnostic ◽  
Artificial Cells

AbstractMicrofluidic droplet generation affords precise, low volume, high throughput opportunities for molecular diagnostics. Isothermal DNA amplification with bioluminescent detection is a fast, low-cost, highly specific molecular diagnostic technique that is triggerable by temperature. Combining loop-mediated isothermal nucleic acid amplification (LAMP) and bioluminescent assay in real time (BART), with droplet microfluidics, should enable high-throughput, low copy, sequence-specific DNA detection by simple light emission. Stable, uniform LAMP–BART droplets are generated with low cost equipment. The composition and scale of these droplets are controllable and the bioluminescent output during DNA amplification can be imaged and quantified. Furthermore these droplets are readily incorporated into encapsulated droplet interface bilayers (eDIBs), or artificial cells, and the bioluminescence tracked in real time for accurate quantification off chip. Microfluidic LAMP–BART droplets with high stability and uniformity of scale coupled with high throughput and low cost generation are suited to digital DNA quantification at low template concentrations and volumes, where multiple measurement partitions are required. The triggerable reaction in the core of eDIBs can be used to study the interrelationship of the droplets with the environment and also used for more complex chemical processing via a self-contained network of droplets, paving the way for smart soft-matter diagnostics.

scholarly journals Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics

RSC Advances ◽  
2020 ◽  
Vol 10 (51) ◽  
pp. 30975-30981
Author(s):  
Adrian M. Nightingale ◽  
Sammer-ul Hassan ◽  
Kyriacos Makris ◽  
Wahida T. Bhuiyan ◽  
Terry J. Harvey ◽  
...  
Keyword(s):  
Droplet Microfluidics ◽  
Microfluidic Chips ◽  
Absorbance Measurement ◽  
Accessible Method

We present a widely accessible method for fabricating monolithic fluoropolymer microfluidic chips, which allows droplet absorbance measurement over multi-month periods.

Validation of the 3M Molecular Detection System for the Detection of Listeria in Meat, Seafood, Dairy, and Retail Environments

2013 ◽  
Vol 76 (5) ◽  
pp. 874-878 ◽  
Author(s):  
ESTHER D. FORTES ◽  
JOHN DAVID ◽  
BOB KOERITZER ◽  
MARTIN WIEDMANN
Keyword(s):  
Foodborne Pathogens ◽  
Molecular Detection ◽  
Detection System ◽  
Dna Amplification ◽  
Cross Contamination ◽  
Chi Square ◽  
Cultural Method ◽  
Detection Assay ◽  
Processing Plants ◽  
Sample Set

There is a continued need to develop improved rapid methods for detection of foodborne pathogens. The aim of this project was to evaluate the 3M Molecular Detection System (3M MDS), which uses isothermal DNA amplification, and the 3M Molecular Detection Assay Listeria using environmental samples obtained from retail delicatessens and meat, seafood, and dairy processing plants. Environmental sponge samples were tested for Listeria with the 3M MDS after 22 and 48 h of enrichment in 3M Modified Listeria Recovery Broth (3M mLRB); enrichments were also used for cultural detection of Listeria spp. Among 391 samples tested for Listeria, 74 were positive by both the 3M MDS and the cultural method, 310 were negative by both methods, 2 were positive by the 3M MDS and negative by the cultural method, and one sample was negative by the 3M MDS and positive by the cultural method. Four samples were removed from the sample set, prior to statistical analyses, due to potential cross-contamination during testing. Listeria isolates from positive samples represented L. monocytogenes, L. innocua, L. welshimeri, and L. seeligeri. Overall, the 3M MDS and culture-based detection after enrichment in 3M mLRB did not differ significantly (P > 0.05) with regard to the number of positive samples, when chi-square analyses were performed for (i) number of positive samples after 22 h, (ii) number of positive samples after 48 h, and (iii) number of positive samples after 22 and/or 48 h of enrichment in 3M mLRB. Among 288 sampling sites that were tested with duplicate sponges, 67 each tested positive with the 3M MDS and the traditional U.S. Food and Drug Administration Bacteriological Analytical Manual method, further supporting that the 3M MDS performs equivalently to traditional methods when used with environmental sponge samples.

scholarly journals Pressure-Free Assembling of Poly(Methyl Methacrylate) Microdevices via Microwave-Assisted Solvent Bonding and Its Biomedical Applications

Biosensors ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 526
Author(s):  
Kieu The Loan Trinh ◽  
Woo Ri Chae ◽  
Nae Yoon Lee
Keyword(s):  
Acetic Acid ◽  
Methyl Methacrylate ◽  
Biomedical Applications ◽  
External Pressure ◽  
Microfluidic Chips ◽  
Poly Methyl Methacrylate ◽  
Bonding Performance ◽  
Passive Micromixer ◽  
Solvent Bonding ◽  
Short Time

Poly(methyl methacrylate) (PMMA) has become an appealing material for manufacturing microfluidic chips, particularly for biomedical applications, because of its transparency and biocompatibility, making the development of an appropriate bonding strategy critical. In our research, we used acetic acid as a solvent to create a pressure-free assembly of PMMA microdevices. The acetic acid applied between the PMMA slabs was activated by microwave using a household microwave oven to tightly merge the substrates without external pressure such as clamps. The bonding performance was tested and a superior bond strength of 14.95 ± 0.77 MPa was achieved when 70% acetic acid was used. Over a long period, the assembled PMMA device with microchannels did not show any leakage. PMMA microdevices were also built as a serpentine 2D passive micromixer and cell culture platform to demonstrate their applicability. The results demonstrated that the bonding scheme allows for the easy assembly of PMMAs with a low risk of clogging and is highly biocompatible. This method provides for a simple but robust assembly of PMMA microdevices in a short time without requiring expensive instruments.

Portable and amplicon contamination prevention cartridges for DNA amplification coupled to lateral flow detection

2015 ◽  
Vol 7 (9) ◽  
pp. 3692-3696 ◽  
Author(s):  
Rui Wang ◽  
Fang Zhang ◽  
Zunzhong Ye ◽  
Jian Wu ◽  
Liu Wang ◽  
...  
Keyword(s):  
Dna Amplification ◽  
Lateral Flow ◽  
Cross Contamination ◽  
Flow Detection

With an enclosed and portable LFD cartridge, GMO can be tested specifically without the generation of cross-contamination.

scholarly journals OsciDrop: A versatile on-demand droplet generator

2021 ◽  
Author(s):  
Shun Ye ◽  
Xu Zheng ◽  
Caiming Li ◽  
Weihang Huang ◽  
Yi Tao ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Droplet Microfluidics ◽  
Droplet Generation ◽  
Microfluidic Chips ◽  
Droplet Generator ◽  
Dna Templates ◽  
On Demand ◽  
High Uniformity ◽  
Monodisperse Droplets

Droplet microfluidics has become a powerful tool in many biological and clinical applications to high-throughput encapsulate reactions with single-cell and single-molecular resolutions. Microfluidic chips, such as flow-focusing droplet generators, have become commonplace in microfluidic laboratories. However, the expensive and precision-demanding microfabrication hinders their widespread use in many biomedical laboratories and clinical facilities. Herein, we present a versatile chip-free droplet generator, termed as OsciDrop, for on-demand generating size-tunable droplets with high uniformity. OsciDrop segments the fluid flowing out of the orifice of a micropipette tip into droplets by oscillating the tip under the surface of a continuous oil phase. We investigated the factors influencing droplet generation by examining several control parameters. Results show that flow rate, oscillating amplitude, and frequency are key parameters to on-demand generate monodisperse droplets. Flexible, repeatable droplet generation by OsciDrop was successfully achieved. Importantly, using an optimal asymmetrical oscillation waveform, OsciDrop can controllably generate monodisperse droplets spanning a wide volume range (200 pL - 2 μL). To demonstrate the capability of OsciDrop for chip-free droplet assays, a digital loop-mediated isothermal amplification (dLAMP) was performed to absolutely quantify African swine fever virus (ASFV) DNA templates. The OsciDrop method opens up a feasible and versatile avenue to perform droplet-based assays, exhibiting full accessibility for chip-free droplet microfluidics.

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