scholarly journals Low Distortion Solvent Bonding of Microfluidic Chips

2016 ◽  
Vol 141 ◽  
pp. 130-137 ◽  
Author(s):  
Shu Pei Ng ◽  
Florencia Edith Wiria ◽  
Nam Beng Tay
Keyword(s):  
Microfluidic Chips ◽  
Low Distortion ◽  
Solvent Bonding

Production of Fluoropolymer Microchips for Droplet Microfluidics and DNA Amplification

2010 ◽  
Author(s):  
Stefano Begolo ◽  
Guillaume Colas ◽  
Laurent Malaquin ◽  
Jean-Louis Viovy
Keyword(s):  
Low Temperatures ◽  
Dna Amplification ◽  
Droplet Microfluidics ◽  
Cross Contamination ◽  
Uniform Pressure ◽  
Microfluidic Chips ◽  
Fabrication Technique ◽  
Flexible Membrane ◽  
Low Surface Energy ◽  
Solvent Bonding

In this paper we present a novel fabrication technique for production of monolithic microfluidic chips made from a fluoropolymer (Dyneon THV). This material retains numerous properties of commonly used fluoropolymers (low surface energy and compatibility with chemicals such as organic solvents or fluorinated oil), and is easily processable at relatively low temperatures (lower than 180°C). We used hot embossing to mold microstructures on flat sheets of this polymer. The microchips are sealed through a combination of thermal and solvent bonding by applying uniform pressure with a flexible membrane. These closed channels can be used for the production and circulation of aqueous droplets in fluorinated oil. This droplet microfluidic configuration is suitable for DNA amplification since it avoids cross contamination between adjacent droplets.

scholarly journals Microfabrication of Nonplanar Polymeric Microfluidics

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 491
Author(s):  
Pin-Chuan Chen ◽  
Chung-Ying Lee ◽  
Lynh Duong
Keyword(s):  
Dimensional Space ◽  
Three Dimensional ◽  
Microlens Array ◽  
Microfluidic Chips ◽  
Pmma Substrate ◽  
In Vitro Characterization ◽  
First Case ◽  
Definition Of ◽  
Solvent Bonding

For four decades, microfluidics technology has been used in exciting, state-of-the-art applications. This paper reports on a novel fabrication approach in which micromachining is used to create nonplanar, three-dimensional microfluidic chips for experiments. Several parameters of micromachining were examined to enhance the smoothness and definition of surface contours in the nonplanar poly(methyl methacrylate) (PMMA) mold inserts. A nonplanar PMMA/PMMA chip and a nonplanar polydimethylsiloxane (PDMS)/PMMA chip were fabricated to demonstrate the efficacy of the proposed approach. In the first case, a S-shape microchannel was fabricated on the nonplanar PMMA substrate and sealed with another nonplanar PMMA via solvent bonding. In the second case, a PDMS membrane was casted from two nonplanar PMMA substrates and bonded on hemispherical PMMA substrate via solvent bonding for use as a microlens array (MLAs). These examples demonstrate the effectiveness of micromachining in the fabrication of nonplanar microfluidic chips directly on a polymeric substrate, as well as in the manufacture of nonplanar mold inserts for use in creating PDMS/PMMA microfluidic chips. This technique facilitates the creation of nonplanar microfluidic chips for applications requiring a three-dimensional space for in vitro characterization.

scholarly journals Pressure-Free Assembling of Poly(Methyl Methacrylate) Microdevices via Microwave-Assisted Solvent Bonding and Its Biomedical Applications

Biosensors ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 526
Author(s):  
Kieu The Loan Trinh ◽  
Woo Ri Chae ◽  
Nae Yoon Lee
Keyword(s):  
Acetic Acid ◽  
Methyl Methacrylate ◽  
Biomedical Applications ◽  
External Pressure ◽  
Microfluidic Chips ◽  
Poly Methyl Methacrylate ◽  
Bonding Performance ◽  
Passive Micromixer ◽  
Solvent Bonding ◽  
Short Time

Poly(methyl methacrylate) (PMMA) has become an appealing material for manufacturing microfluidic chips, particularly for biomedical applications, because of its transparency and biocompatibility, making the development of an appropriate bonding strategy critical. In our research, we used acetic acid as a solvent to create a pressure-free assembly of PMMA microdevices. The acetic acid applied between the PMMA slabs was activated by microwave using a household microwave oven to tightly merge the substrates without external pressure such as clamps. The bonding performance was tested and a superior bond strength of 14.95 ± 0.77 MPa was achieved when 70% acetic acid was used. Over a long period, the assembled PMMA device with microchannels did not show any leakage. PMMA microdevices were also built as a serpentine 2D passive micromixer and cell culture platform to demonstrate their applicability. The results demonstrated that the bonding scheme allows for the easy assembly of PMMAs with a low risk of clogging and is highly biocompatible. This method provides for a simple but robust assembly of PMMA microdevices in a short time without requiring expensive instruments.

Analysis of the Dynamics of the Electric Capacitance of a Cell Monolayer at the Late Stages of Caco-2 cell Differentiation

2019 ◽  
Vol 35 (6) ◽  
pp. 87-90
Author(s):  
S.V. Nikulin ◽  
V.A. Petrov ◽  
D.A. Sakharov
Keyword(s):  
Impedance Spectroscopy ◽  
Cell Monolayer ◽  
Microfluidic Chips ◽  
Russian Science ◽  
Electric Capacitance ◽  
A Cell ◽  
Static Conditions ◽  
Late Stages

The real-time monitoring of electric capacitance (impedance spectroscopy) allowed obtaining evidence that structures which look like intestinal villi can be formed during the cultivation under static conditions as well as during the cultivation in microfluidic chips. It was shown in this work via transcriptome analysis that the Hh signaling pathway is involved in the formation of villus-like structures in vitro, which was previously shown for their formation in vivo. impedance spectroscopy, intestine, villi, electric capacitance, Hh The study was funded by the Russian Science Foundation (Project 16-19-10597).

Biomedical Devices for Pathogen Detection Using Microfluidic Chips

2014 ◽  
Vol 11 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Ming-Yuan-Lee ◽  
Chih-Hui Yang ◽  
Keng-Shiang Huang
Keyword(s):  
Pathogen Detection ◽  
Biomedical Devices ◽  
Microfluidic Chips

scholarly journals DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dezhi Feng ◽  
Jing Su ◽  
Yi Xu ◽  
Guifang He ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Reaction Time ◽  
Electrochemical Detection ◽  
Detection System ◽  
Detection Performance ◽  
Pdms Layer ◽  
Microfluidic Chips ◽  
Serum Samples ◽  
Gold Nanoflowers ◽  
Dna Tetrahedron

AbstractProstate-specific antigen (PSA) is the most widely used biomarker for the early diagnosis of prostate cancer. Existing methods for PSA detection are burdened with some limitations and require improvement. Herein, we developed a novel microfluidic–electrochemical (μFEC) detection system for PSA detection. First, we constructed an electrochemical biosensor based on screen-printed electrodes (SPEs) with modification of gold nanoflowers (Au NFs) and DNA tetrahedron structural probes (TSPs), which showed great detection performance. Second, we fabricated microfluidic chips by DNA TSP-Au NF-modified SPEs and a PDMS layer with designed dense meandering microchannels. Finally, the μFEC detection system was achieved based on microfluidic chips integrated with the liquid automatic conveying unit and electrochemical detection platform. The μFEC system we developed acquired great detection performance for PSA detection in PBS solution. For PSA assays in spiked serum samples of the μFEC system, we obtained a linear dynamic range of 1–100 ng/mL with a limit of detection of 0.2 ng/mL and a total reaction time <25 min. Real serum samples of prostate cancer patients presented a strong correlation between the “gold-standard” chemiluminescence assays and the μFEC system. In terms of operation procedure, cost, and reaction time, our method was superior to the current methods for PSA detection and shows great potential for practical clinical application in the future.

Kinetic Control of Length and Morphology of Segmented Polymeric Nanofibers in Microfluidic Chips

Langmuir ◽  
2020 ◽  
Vol 36 (44) ◽  
pp. 13364-13370
Author(s):  
Zhengping Tan ◽  
Zaiyan Hou ◽  
Ke Wang ◽  
Yuce Li ◽  
Lianbin Zhang ◽  
...  
Keyword(s):  
Kinetic Control ◽  
Microfluidic Chips ◽  
Polymeric Nanofibers

scholarly journals Strategy for fast manufacturing of 3D hydrodynamic focusing multilayer microfluidic chips and its application for flow-based synthesis of gold nanoparticles

2021 ◽  
Vol 25 (8) ◽  
Author(s):  
Yanwei Wang ◽  
Michael Seidel
Keyword(s):  
Gold Nanoparticles ◽  
Microfluidic Devices ◽  
Microfluidic Chips ◽  
Proof Of Concept ◽  
Hydrodynamic Focusing ◽  
Pressure Sensitive Adhesive ◽  
Pmma Sheet ◽  
Pressure Sensitive ◽  
Working Device ◽  
Channel Structures

AbstractFabrication of 3D microfluidic devices is normally quite expensive and tedious. A strategy was established to rapidly and effectively produce multilayer 3D microfluidic chips which are made of two layers of poly(methyl methacrylate) (PMMA) sheets and three layers of double-sided pressure sensitive adhesive (PSA) tapes. The channel structures were cut in each layer by cutting plotter before assembly. The structured channels were covered by a PMMA sheet on top and a PMMA carrier which contained threads to connect with tubing. A large variety of PMMA slides and PSA tapes can easily be designed and cut with the help of a cutting plotter. The microfluidic chip was manually assembled by a simple lamination process.The complete fabrication process from device design concept to working device can be completed in minutes without the need of expensive equipment such as laser, thermal lamination, and cleanroom. This rapid frabrication method was applied for design of a 3D hydrodynamic focusing device for synthesis of gold nanoparticles (AuNPs) as proof-of-concept. The fouling of AuNPs was prevented by means of a sheath flow. Different parameters such as flow rate and concentration of reagents were controlled to achieve AuNPs of various sizes. The sheet-based fabrication method offers a possibility to create complex microfluidic devices in a rapid, cheap and easy way.

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