Structural and Mechanical Multi-Scale Characterization of White New Zealand Rabbit Achilles Tendon

2012 ◽  
Author(s):  
Cyril J. F. Kahn ◽  
Dominique Dumas ◽  
Elmira Arab-Tehrany ◽  
Vanessa Marie ◽  
Nguyen Tran ◽  
...  
Keyword(s):  
Second Harmonic ◽  
Dynamic Properties ◽  
Test Machine ◽  
Force Microscopy ◽  
Multi Scale ◽  
Atomic Force ◽  
Linear Viscoelastic ◽  
Scale Characterization ◽  
The Relationship

Tendons and ligaments are complex multi-scale collageneous structures playing a fundamental role in mouvement. Even if these tissues are extensively studied in the past decades, modeling their non-linear viscoelastic properties is still a tough challenge. In order to reveal the relationship between the multi-scale structures and the macroscopic mechanical properties, we used atomic force microscopy (AFM) and second harmonic generation (SHG) microscopy to study unstreateched microtome slices of rabbit Achilles tendons, and an Adamel Lomargy DY.22 tensile test machine to study the dynamic properties of these tissues. Based on our data, a Zener model was used to describe the dynamic loading and unloading cycles.

Structural and Morphological Characterization of Ultrathin Films of an Asymmetric Polydiacetylene

1996 ◽  
Vol 440 ◽  
Author(s):  
H. C. Wang ◽  
D. W. Cheong ◽  
J. Kumar ◽  
C. Sung ◽  
S. K. Tripathy
Keyword(s):  
Second Harmonic ◽  
Morphological Characterization ◽  
Lb Films ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Glass Substrates ◽  
Atomic Force ◽  
Transmission Electron ◽  
Air Water Interface

AbstractA soluble, asymmetrically substituted polydiacetylene, poly(BPOD), has been reported to form stable monolayers at the air-water interface by the Langmuir-Blodgett (LB) technique [2]. Preformed polydiacetylene has been deposited onto hydrophobic substrates as multilayers to form second order nonlinear optical thin films. Second harmonic generation was found to increase with the number of layers. From previous atomic force microscopy (AFM) studies backbone orientation along the dipping direction with an interchain spacing of about 5 A° was indicated [2].The film morphology and preferential molecular orientation of these LB films are further investigated by transmission electron microscopy (TEM). A specifically tailored sample preparation method for the ultrathin LB films was used. Multilayer films were deposited on hydrophobic collodion covered glass substrates for this purpose. Electron diffraction was employed to study the crystalline organization of mono and multilayers of LB films as well as cast films.

Characterization of Asymmetric Polydiacetylene Ultrathin Films

1995 ◽  
Vol 382 ◽  
Author(s):  
D. W. Cheong ◽  
V. Shivshankar ◽  
H. C. Wang ◽  
C. M. Sung ◽  
J. Kumar ◽  
...  
Keyword(s):  
Ultrathin Films ◽  
Second Harmonic ◽  
Film Deposition ◽  
Asymmetric Structure ◽  
Blodgett Film ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Atomic Force ◽  
Ft Ir

ABSTRACTNonlinear optical (NLO) ultrathin films of a preforrned asymmetric polydiacetylene have been fabricated by Z-type Langmuir-Blodgett film deposition from the air-water interface. Induced in-plane orientation of the polydiacetylene backbone on the substrates has been confirmed by UV/Vis, FT-IR dichroism, and degenerate four wave mixing (DFWM) studies. All the measurements indicate that the backbone is prefe rentially oriented along the dipping direction. Second harmonic generation study suggests that the LB multilayers form an asymmetric structure (Z-type) due to the accumulation of 2-dimensional ordered monolayer and the dominant induced second order polarization is in the plane of the film. The film morphology and molecular orientation have been investigated by atomic force microscopy (AFM).

Characterization of epitaxial CVD graphene on Ir(111)/α-Al2O3(0001) by photoelectron momentum microscopy

2022 ◽  
Author(s):  
Eri Hashimoto ◽  
Keigo Tamura ◽  
Hayato Yamaguchi ◽  
Takeshi Watanabe ◽  
Fumihiko Matsui ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Growth Conditions ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cvd Growth ◽  
Grown Graphene ◽  
Α Al2o3 ◽  
The Relationship ◽  
X Ray Absorption

Abstract We characterized CVD-grown graphene with high single-crystallinity on Ir(111)/α-Al2O3(0001) by photoelectron momentum microscopy. A multi-functional photoelectron momentum microscope (PMM), which is installed with element-specific valence band photoelectron spectroscopy and X-ray absorption spectroscopy, is a complementary characterization tool to conventional methods, such as Raman spectroscopy and atomic force microscopy, for comprehensive and quantitative characterization of graphene/Ir(111). Using PMM, we characterized the properties of CVD-grown graphene including the single-crystallinity, number of layers, crystal orientation, and degree of interaction between graphene and Ir(111) and clarified the relationship between these properties and the CVD growth conditions.

Nanometer-Scale Characterization of Ferroelectric Polymer Thin Films by Variable-Temperature Atomic Force Microscopy

2000 ◽  
Vol 39 (Part 1, No. 6B) ◽  
pp. 3830-3833 ◽  
Author(s):  
Takeshi Fukuma ◽  
Kei Kobayashi ◽  
Toshihisa Horiuchi ◽  
Hirofumi Yamada ◽  
Kazumi Matsushige
Keyword(s):  
Thin Films ◽  
Atomic Force Microscopy ◽  
Variable Temperature ◽  
Polymer Thin Films ◽  
Nanometer Scale ◽  
Force Microscopy ◽  
Ferroelectric Polymer ◽  
Atomic Force ◽  
Scale Characterization

Multi-scale characterization of thermoplastic starch structure using Second Harmonic Generation imaging and NMR

2018 ◽  
Vol 194 ◽  
pp. 80-88 ◽  
Author(s):  
Veronica Nessi ◽  
Agnès Rolland-Sabaté ◽  
Denis Lourdin ◽  
Frédéric Jamme ◽  
Chloé Chevigny ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Thermoplastic Starch ◽  
Multi Scale ◽  
Starch Structure ◽  
Scale Characterization ◽  
Second Harmonic Generation Imaging

Nanometer scale characterization of polymer films by atomic-force microscopy

2002 ◽  
Vol 181 (1) ◽  
pp. 457-466 ◽  
Author(s):  
Christian Teichert ◽  
Alfred Haas ◽  
Gernot M. Wallner ◽  
Reinhold W. Lang
Keyword(s):  
Atomic Force Microscopy ◽  
Polymer Films ◽  
Nanometer Scale ◽  
Force Microscopy ◽  
Atomic Force ◽  
Scale Characterization

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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