Single Molecule Analysis Tool (SMAT) for Multiplexed Label-Free Assessment of Rare Cell Populations

Volume 3: Biomedical and Biotechnology Engineering ◽

10.1115/imece2014-40225 ◽

2014 ◽

Author(s):

Anjan Panneer Selvam ◽

Shalini Prasad

Keyword(s):

Lung Cancer ◽

Stem Cell ◽

Single Molecule ◽

Electrochemical Impedance ◽

Stem Cell Marker ◽

Analysis Tool ◽

Label Free ◽

Lung Cancer Stem Cells ◽

Single Molecule Analysis

A nanowell sensor for single molecular proteomic analysis of lung cancer has been designed. The nanowell sensor is an electrochemical immunoassay and comprises of a heterogenous nanoporous arrays integrated on to a gold microelectronic platform. The sensor operates on the principle of electrochemical impedance spectroscopy (EIS). Our approach to classification of lung cancer is based on screening for levels of expression of specific proteomic biomarkers associated with lung cancer stem cells. Proteomic activity for two lung cancer cell lines for two specific markers (ALDH1A1 and ALDH1A3) was quantified. Test samples prepared by synthetically spiking human pooled serum were tested and quantified for cancer stem cell marker activity. The lowest proteomic activity measured with (a) ALDH1A3 was 0.01 ng/mL and (b) ALDH1A1 was 1 ng/mL correlating to the detection of unit stem cell count.

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Label-free classification of neurons and glia in neural stem cell cultures using a hyperspectral imaging microscopy combined with machine learning

Scientific Reports ◽

10.1038/s41598-018-37241-y ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Hiroshi Ogi ◽

Sanzo Moriwaki ◽

Masahiko Kokubo ◽

Yuichiro Hikida ◽

Kyoko Itoh

Keyword(s):

Machine Learning ◽

Stem Cell ◽

Hyperspectral Imaging ◽

Neural Stem Cell ◽

Cell Cultures ◽

Label Free ◽

Free Classification

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PCR-free, microfluidic single molecule analysis of circulating nucleic acids in lung cancer patient serum

2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society ◽

10.1109/iembs.2011.6092070 ◽

2011 ◽

Author(s):

K. J. Liu ◽

Tza-Huei Wang

Keyword(s):

Lung Cancer ◽

Cancer Patient ◽

Nucleic Acids ◽

Single Molecule ◽

Lung Cancer Patient ◽

Circulating Nucleic Acids ◽

Single Molecule Analysis

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Clinical significance of putative cancer stem cell marker CD44 in different histological subtypes of lung cancer

Cancer Biomarkers ◽

10.3233/cbm-140424 ◽

2014 ◽

Vol 14 (6) ◽

pp. 457-467 ◽

Cited By ~ 24

Author(s):

Raheleh Roudi ◽

Zahra Madjd ◽

Alireza Korourian ◽

Mitra Mehrazma ◽

Saadat Molanae ◽

...

Keyword(s):

Lung Cancer ◽

Stem Cell ◽

Cancer Stem Cell ◽

Clinical Significance ◽

Stem Cell Marker ◽

Cancer Stem Cell Marker ◽

Cell Marker ◽

Histological Subtypes

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Significance of stem cell marker Nanog gene in the diagnosis and prognosis of lung cancer

Oncology Letters ◽

10.3892/ol.2016.4923 ◽

2016 ◽

Vol 12 (4) ◽

pp. 2507-2510 ◽

Cited By ~ 5

Author(s):

Zeng Liu ◽

Jing Zhang ◽

Honggang Kang ◽

Guiming Sun ◽

Baozhong Wang ◽

...

Keyword(s):

Lung Cancer ◽

Stem Cell ◽

Stem Cell Marker ◽

Cell Marker ◽

Diagnosis And Prognosis ◽

Nanog Gene

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Mechanistic Exploration of Cancer Stem Cell Marker Voltage-Dependent Calcium Channel α2δ1 Subunit-mediated Chemotherapy Resistance in Small-Cell Lung Cancer

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-17-1932 ◽

2018 ◽

Vol 24 (9) ◽

pp. 2148-2158 ◽

Cited By ~ 15

Author(s):

Jiangyong Yu ◽

Shuhang Wang ◽

Wei Zhao ◽

Jianchun Duan ◽

Zhijie Wang ◽

...

Keyword(s):

Lung Cancer ◽

Stem Cell ◽

Chemotherapy Resistance ◽

Small Cell ◽

Stem Cell Marker ◽

Cancer Stem Cell Marker ◽

Cell Marker ◽

Small Cell Lung ◽

Voltage Dependent Calcium Channel ◽

Voltage Dependent

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Evidence for circulating cancer stem-like cells and epithelial–mesenchymal transition phenotype in the pleurospheres derived from lung adenocarcinoma using liquid biopsy

Tumor Biology ◽

10.1177/1010428317695915 ◽

2017 ◽

Vol 39 (3) ◽

pp. 101042831769591 ◽

Cited By ~ 6

Author(s):

Sheefa Mirza ◽

Nayan Jain ◽

Rakesh Rawal

Keyword(s):

Lung Cancer ◽

Stem Cells ◽

Stem Cell ◽

Cancer Stem Cells ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Epithelial Mesenchymal Transition ◽

Tumor Stem Cells ◽

Mesenchymal Transition ◽

Lung Cancer Stem Cells

Lung cancer stem cells are supposed to be the main drivers of tumor initiation, maintenance, drug resistance, and relapse of the disease. Hence, identification of the cellular and molecular aspects of these cells is a prerequisite for targeted therapy of lung cancer. Currently, analysis of circulating tumor cells has the potential to become the main diagnostic technique to monitor disease progression or therapeutic response as it is non-invasive. However, accurate detection of circulating tumor cells has remained a challenge, as epithelial cell markers used so far are not always trustworthy for detecting circulating tumor cells, especially during epithelial–mesenchymal transition. As cancer stem cells are the only culprit to initiate metastatic tumors, our aim was to isolate and characterize circulating tumor stem cells rather than circulating tumor cells from the peripheral blood of NSCLC adenocarcinoma as limited data are available addressing the gene expression profiling of lung cancer stem cells. Here, we reveal that CD44(+)/CD24(−) population in circulation not only exhibit stem cell–related genes but also possess epithelial–mesenchymal transition characteristics. In conclusion, the use of one or more cancer stem cell markers along with epithelial, mesenchymal and epithelial mesenchymal transition markers will prospectively provide the most precise assessment of the threat for recurrence and metastatic disease and has a great potential for forthcoming applications in harvesting circulating tumor stem cells and their downstream applications. Our results will aid in developing diagnostic and prognostic modalities and personalized treatment regimens like dendritic cell–based immunotherapy that can be utilized for targeting and eliminating circulating tumor stem cells, to significantly reduce the possibility of relapse and improve clinical outcomes.

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Polymorphisms of the Stem Cell Marker Gene CD133 and the Risk of Lung Cancer in Chinese Population

Lung ◽

10.1007/s00408-016-9876-1 ◽

2016 ◽

Vol 194 (3) ◽

pp. 393-400 ◽

Cited By ~ 4

Author(s):

Qing-Feng Liu ◽

Zhi-Fei Zhang ◽

Guang-Jie Hou ◽

Guang-Yu Yang ◽

Yi He

Keyword(s):

Lung Cancer ◽

Stem Cell ◽

Chinese Population ◽

Marker Gene ◽

Stem Cell Marker ◽

Cell Marker

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Lung Cancer Stem Cells

Disease Markers ◽

10.1155/2008/396281 ◽

2008 ◽

Vol 24 (4-5) ◽

pp. 257-266 ◽

Cited By ~ 26

Author(s):

Sharon R. Pine ◽

Blair Marshall ◽

Lyuba Varticovski

Keyword(s):

Lung Cancer ◽

Stem Cells ◽

Stem Cell ◽

Cancer Stem Cells ◽

Cell Signaling ◽

Current Knowledge ◽

Lung Cancer Stem Cells ◽

Multipotent Differentiation ◽

Cancer Types ◽

Active Research

Lung cancer remains a major cause of cancer-related lethality because of high incidence and recurrence in spite of significant advances in staging and therapies. Recent data indicates that stem cells situated throughout the airways may initiate cancer formation. These putative stem cells maintain protumorigenic characteristics including high proliferative capacity, multipotent differentiation, drug resistance and long lifespan relative to other cells. Stem cell signaling and differentiation pathways are maintained within distinct cancer types, and destabilization of this machinery may participate in maintenance of cancer stem cells. Characterization of lung cancer stem cells is an area of active research and is critical for developing novel therapies. This review summarizes the current knowledge on stem cell signaling pathways and cell markers used to identify the lung cancer stem cells.

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