Evaluation of a Novel Scaffold Material for Tendon Tissue Engineering

2007 ◽  
Author(s):  
Victor S. Nirmalanandhan ◽  
Kirsten R. C. Kinneberg ◽  
Natalia Juncosa-Melvin ◽  
Heather M. Powell ◽  
Marepalli Rao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Type I Collagen ◽  
Tendon Repair ◽  
Maximum Force ◽  
Attractive Alternative ◽  
Mechanical Resistance ◽  
Type I ◽  
Novel Scaffold

Tissue engineering offers an attractive alternative to direct repair or reconstruction of soft tissue injuries. Tissue engineered constructs containing mesenchymal stem cells (MSCs) seeded in commercially available type I collagen sponges (P1076, Kensey Nash Corporation, Exton, PA) are currently being used within our laboratory to repair tendon injuries in rabbit models [1]. When introduced into the wound site, mechanically stimulated stem cell-collagen sponge constructs exhibit 50% greater maximum force and stiffness at 12 weeks compared to values for static controls [1]. However, these constructs often lack the maximum force sufficient to resist the peak in vivo forces acting on the repair site [2, 3]. Insufficient repair biomechanics can be attributed to the poor initial mechanical resistance provided by the collagen sponges to replace the function of the lost tendon before its degradation and replacement with new extracellular matrix. This current study seeks to identify a biologically-derived scaffold with improved mechanical integrity that could be used in stem cell-based tissue engineered constructs for tendon repair.

Application of Tissue Engineering Techniques for Rotator Cuff Regeneration Using a Chitosan-Based Hyaluronan Hybrid Fiber Scaffold

2005 ◽  
Vol 33 (8) ◽  
pp. 1193-1201 ◽  
Author(s):  
Tadanao Funakoshi ◽  
Tokifumi Majima ◽  
Norimasa Iwasaki ◽  
Naoki Suenaga ◽  
Naohiro Sawaguchi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Rotator Cuff ◽  
Mechanical Strength ◽  
Type I Collagen ◽  
Tangent Modulus ◽  
Rotator Cuff Tears ◽  
Type I ◽  
Scaffold Materials ◽  
Novel Scaffold

Background The current surgical procedures for irreparable rotator cuff tears have considerable limitations. Tissue engineering techniques using novel scaffold materials offer potential alternatives for managing these conditions. Hypothesis A chitosan-based hyaluronan hybrid scaffold could enhance type I collagen products with seeded fibroblasts and thereby increase the mechanical strength of regenerated tendon in vivo. Study Design Controlled laboratory study. Methods The scaffolds were created from chitosan-based hyaluronan hybrid polymer fibers. Forty-eight rabbit infraspinatus tendons and their humeral insertions were removed to create defects. Each defect was covered with a fibroblast-seeded scaffold (n = 16) or a non-fibroblast-seeded scaffold (n = 16). In the other 16 shoulders, the rotator cuff defect was left free as the control. At 4 and 12 weeks after surgery, the engineered tendons were assessed by histological, immunohistochemical (n = 2), and biomechanical (n = 6) analyses. Results Type I collagen was only seen in the fibroblast-seeded scaffold and increased in the regenerated tissue. The tensile strength and tangent modulus in the fibroblast-seeded scaffold were significantly improved from 4 to 12 weeks postoperatively. The fibroblast-seeded scaffold had a significantly greater tangent modulus than did the non-fibroblast-seeded scaffold and the control at 12 weeks. Conclusion This scaffold material enhanced the production of type I collagen and led to improved mechanical strength in the regenerated tissues of the rotator cuff in vivo. Clinical Relevance Rotator cuff regeneration is feasible using this tissue engineering technique.

Combined Effect of Glycosaminoglycan and Mechanical Stimulation on the In Vitro Biomechanics of Tissue Engineered Tendon Constructs

2007 ◽  
Author(s):  
Kirsten R. C. Kinneberg ◽  
Victor S. Nirmalanandhan ◽  
Heather M. Powell ◽  
Steven T. Boyce ◽  
David L. Butler
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Rabbit Model ◽  
The United States ◽  
Maximum Force ◽  
Attractive Alternative ◽  
Type I ◽  
Post Surgery ◽  
Scaffold Materials

Tissue engineering offers an attractive alternative to direct repair or reconstruction of injuries to tendons, ligaments and capsular structures that represent almost 45% of the 32 million musculoskeletal injuries that occur each year in the United States [1]. Mesenchymal stem cell (MSC)-seeded collagen constructs are currently being used by our group to repair tendon injuries in the rabbit model [2, 3]. Although these cell-assisted repairs exhibit 50% greater maximum force and stiffness at 12 weeks compared to values for natural repair, tissues often lack the maximum force sufficient to resist the peak in vivo forces acting on the repair site [3]. Our laboratory has previously demonstrated that in vitro construct stiffness and repair stiffness at 12 weeks post surgery are positively correlated [4]. Therefore, in an effort to further improve the repair outcome using tissue engineering, we continue our investigation of scaffold materials to create stiffer MSC-collagen constructs. Our group has recently evaluated two scaffold materials, type I collagen sponges fabricated within the Engineered Skin Lab (ESL, Shriners Hospitals for Children) by a freezing and lyophilization process with and without glycosaminoglycan (chondroitin-6-sulfate; GAG) [5] and found the ESL sponges to significantly improve biomechanical properties of the constructs compared to sponges we currently use in the lab (P1076, Kensey Nash Corporation, Exton, PA). This study also demonstrated that GAG significantly upregulates collagen type I, decorin, and fibronectin gene expression (unpublished results).

scholarly journals Estrogen-induced breast cancer is the result of disruption of asymmetric cell division of the stem cell

2010 ◽  
Vol 1 (2) ◽  
Author(s):  
Jose Russo ◽  
Kara Snider ◽  
Julia S. Pereira ◽  
Irma H. Russo
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Type I Collagen ◽  
Asymmetric Cell Division ◽  
Type I ◽  
In Vivo Models ◽  
Pregnancy And Lactation ◽  
In Vitro Systems

AbstractStem cells have the unique potential to divide asymmetrically to generate daughters with distinct fates, one which remains a stem cell and the other which turns into a cell committed to differentiation. By dividing asymmetrically, stem cells maintain the stem cell pool and simultaneously generate committed cells that reconstitute the organ, for example, to prepare the breast for a new pregnancy after involution from a previous pregnancy and lactation process. In addition to the in vivo models of mammary morphogenesis, there are in vitro systems that make the ductulogenic pattern of breast epithelia growth more amenable to study in critically determined conditions. The human breast epithelial cells MCF-10F formed tubules when grown in type I collagen and we demonstrated that treatment of these cells with 17β-estradiol (E

scholarly journals Tenogenically differentiated adipose-derived stem cells are effective in Achilles tendon repair in vivo

2018 ◽  
Vol 9 ◽  
pp. 204173141881118 ◽  
Author(s):  
Jolanta B Norelli ◽  
Dawid P Plaza ◽  
Drew N Stal ◽  
Anish M Varghese ◽  
Haixiang Liang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Collagen Type ◽  
Tendon Repair ◽  
Collagen Type I ◽  
Platelet Derived Growth Factor ◽  
Type I ◽  
Adipose Derived Stem Cells ◽  
Adipose Derived Stem Cell

The purpose of this study was to characterize rat adipose-derived stem cells, induce adipose-derived stem cell tenogenesis, and analyze adipose-derived stem cell effects on tendon repair in vivo. Adipose-derived stem cells demonstrated an immunomodulatory, pro-angiogenic, and pro-proliferatory profile in vitro. Tenogenesis was induced for 1, 7, 14, and 21 days with 24 combinations of growth differentiation factor-5, 6, and 7 and platelet-derived growth factor–BB. Adipose-derived stem cells expression of scleraxis and collagen type I increased the most after 14 days of induction with growth differentiation factor-6 and platelet-derived growth factor–BB. Achilles excision defects injected with hydrogel alone (Gp2), with undifferentiated (Gp3) adipose-derived stem cells, or tenogenically differentiated (Gp4) adipose-derived stem cells exhibited improved tissue repair compared with untreated tendons (Gp1). Addition of adipose-derived stem cells improved tissue cytoarchitecture and increased expression of collagen type I and III, scleraxis, and tenomodulin. Adipose-derived stem cells significantly improved biomechanical properties (ultimate load and elastic toughness) over time more than hydrogel alone, while tenogenically differentiated adipose-derived stem cells improved the mean histological score and collagen fiber dispersion range closest to normal tendon. In addition, tendon sections treated with GFP-adipose-derived stem cells exhibited green fluorescence and positive GFP immunostaining on microscopy confirming the in vivo survival of adipose-derived stem cells that were injected into tendon defects to support the effects of adipose-derived stem cells on tissue up to 4.5 weeks post injury.

scholarly journals Human Stem Cell Derived Osteocytes in Bone-on-Chip

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1443-1455
Author(s):  
E. Budyn ◽  
N. Gaci ◽  
S. Sanders ◽  
M. Bensidhoum ◽  
E. Schmidt ◽  
...  
Keyword(s):  
Stem Cell ◽  
Type I Collagen ◽  
Mechanical Load ◽  
Human Bone ◽  
Type I ◽  
Native Bone ◽  
On Chip

ABSTRACTHuman mesenchymal stem cells were reseeded in decellularized human bone subject to a controlled mechanical loading to create a bone-on-chip that was cultured for over 26 months. The cell morphology and their secretome were characterized using immunohistochemistry and in situ immunofluorescence under confocal microscopy. The presence of stem cell derived osteocytes was confirmed at 547 days. Different cell populations were identified. Some cells were connected by long processes and formed a network. Comparison of the MSCs in vitro reorganization and calcium response to in situ mechanical stimulation were compared to MLOY4 cells reseeded on human bone. The bone-on-chip produced an ECM of which the strength was nearly a quarter of native bone after 109 days and that contained calcium minerals at 39 days and type I collagen at 256 days. The cytoplasmic calcium concentration variations seemed to adapt to the expected in vivo mechanical load at the successive stages of cell differentiation in agreement with studies using fluid shear flow stimulation. Some degree of bone-like formation over a long period of time with the formation of a newly formed matrix was observed.

scholarly journals Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

2021 ◽  
Vol 8 (3) ◽  
pp. 39
Author(s):  
Britani N. Blackstone ◽  
Summer C. Gallentine ◽  
Heather M. Powell
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Collagen Type I ◽  
The Body ◽  
Pool Data ◽  
Type I ◽  
High Concentrations ◽  
Increased Strength

Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.

In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

2021 ◽  
pp. 039139882110255
Author(s):  
Sara Anajafi ◽  
Azam Ranjbar ◽  
Monireh Torabi-Rahvar ◽  
Naser Ahmadbeigi
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cultured Cells ◽  
Morphological Evidence ◽  
Cell Aggregates ◽  
Plla Scaffold ◽  
Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

scholarly journals Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays

2021 ◽  
Vol 19 ◽  
pp. 228080002198969
Author(s):  
Min-Xia Zhang ◽  
Wan-Yi Zhao ◽  
Qing-Qing Fang ◽  
Xiao-Feng Wang ◽  
Chun-Ye Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Type I Collagen ◽  
Inhibition Zone ◽  
Negative Control ◽  
Type I ◽  
Nih3t3 Cells ◽  
Post Operation

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

scholarly journals Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 775
Author(s):  
Olimpia Ortiz-Arrabal ◽  
Ramón Carmona ◽  
Óscar-Darío García-García ◽  
Jesús Chato-Astrain ◽  
David Sánchez-Porras ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Ex Vivo ◽  
Cartilage Damage ◽  
Human Mesenchymal Stem Cells ◽  
In Silico Analysis ◽  
Cartilage Tissue ◽  
Advanced Therapy Medicinal Product ◽  
Advanced Therapy ◽  
Novel Scaffold

Because cartilage has limited regenerative capability, a fully efficient advanced therapy medicinal product is needed to treat severe cartilage damage. We evaluated a novel biomaterial obtained by decellularizing sturgeon chondral endoskeleton tissue for use in cartilage tissue engineering. In silico analysis suggested high homology between human and sturgeon collagen proteins, and ultra-performance liquid chromatography confirmed that both types of cartilage consisted mainly of the same amino acids. Decellularized sturgeon cartilage was recellularized with human chondrocytes and four types of human mesenchymal stem cells (MSC) and their suitability for generating a cartilage substitute was assessed ex vivo and in vivo. The results supported the biocompatibility of the novel scaffold, as well as its ability to sustain cell adhesion, proliferation and differentiation. In vivo assays showed that the MSC cells in grafted cartilage disks were biosynthetically active and able to remodel the extracellular matrix of cartilage substitutes, with the production of type II collagen and other relevant components, especially when adipose tissue MSC were used. In addition, these cartilage substitutes triggered a pro-regenerative reaction mediated by CD206-positive M2 macrophages. These preliminary results warrant further research to characterize in greater detail the potential clinical translation of these novel cartilage substitutes.

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