Dynamic Culture Improves Mechanical Functionality of MSC-Laden Tissue Engineered Constructs in a Depth-Dependent Manner

2011 ◽  
Author(s):  
Megan J. Farrell ◽  
Eric S. Comeau ◽  
Robert L. Mauck
Keyword(s):  
Mechanical Properties ◽  
Local Equilibrium ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Nutrient Supply ◽  
Cartilage Tissue ◽  
Dependent Manner ◽  
Depth Dependence ◽  
Dynamic Culture ◽  
The Impact

Limitations associated with the use of autologous chondrocytes (CH) for cartilage tissue engineering beget the need for alternative cell sources. Mesenchymal stem cells (MSC) are clinically attractive due to their ability to undergo chondrogenesis in three-dimensional culture [1,2]; however, when compared to CH, MSC fail to develop functional equivalence [2,3]. We have previously shown a marked depth-dependence in local equilibrium modulus of MSC-laden gels, with the superficial zones (where maximal media exchange occurs) considerably stiffer than regions removed from nutrient supply (center and bottom of construct); less dramatic depth-dependence was observed in CH-laden gels [4]. Similarly, other studies have shown depth-dependent properties in CH-laden gels with the construct edge generally stiffer than the center [5]. Given this apparent influence of nutrient supply, the objective of the current study was to assess the impact of dynamic culture (via orbital shaking) on the development of depth-dependent mechanical properties in both MSC and CH-laden hydrogels. Furthermore, we assessed cell viability and matrix content throughout the construct depth to determine the mechanism by which this depth-dependency arises. We hypothesized that improved nutrient transport would reduce construct inhomogeneity (particularly for MSC-laden constructs) and improve bulk mechanical properties.

scholarly journals PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Liangquan Peng ◽  
Yong He ◽  
Weimin Zhu ◽  
Wei Lu ◽  
Yong Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Low Temperature ◽  
Composite Scaffold ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Low Temperature Deposition ◽  
Temperature Deposition

Abstract Background Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering. Methods The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined. Results Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold. Conclusion The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.

Tissue-Engineered Cartilage in Three-Dimensional Cultured Chondrocytes by Ultrasound Stimulation and Collagen Sponge as Scaffold

2009 ◽  
Author(s):  
Toshihiko Shiraishi ◽  
Ietomo Matsunaga ◽  
Shin Morishita ◽  
Ryohei Takeuchi ◽  
Tomoyuki Saito ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Three Dimensional ◽  
Collagen Sponge ◽  
Cartilage Tissue ◽  
Mechanical Environment ◽  
Ultrasound Stimulation ◽  
Scaffold Structure ◽  
Matrix Production ◽  
Engineered Cartilage ◽  
Cultured Chondrocytes

This paper describes the effects of ultrasound stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen sponges as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis). It has been shown that cell proliferation and matrix production can be facilitated by considering the mechanical environment of the cultured chondrocytes and the mechanical properties of the scaffold structure used in the culture and of the stimulation used.

Experimental Study on Mechanical Properties of Rubber Used for Damping Bearing

2011 ◽  
Vol 189-193 ◽  
pp. 1132-1136 ◽  
Author(s):  
Yong Xu Zhao ◽  
Wen Jun Hu ◽  
Jun Mei ◽  
Niu Wei ◽  
Jian Jun Xie
Keyword(s):  
Mechanical Properties ◽  
Low Temperature ◽  
Three Dimensional ◽  
Middle Part ◽  
Loading Conditions ◽  
Deflection Curve ◽  
Rubber Bearing ◽  
Components Analysis ◽  
The Impact ◽  
Load Deflection Curve

After testing on T-type rubber bearing under tensile, compression and shear mechanical properties under different temperature in this paper. Obtained load deflection curve and destructive mode under different loading conditions at -40 and normal temperature of rubber components. Analysis the impact of temperature and the loading conditions that effect on load-elongation and destructive mode of T-type damping rubber structure. It showed that T-end rubber bearing has different kinds of deformation under different force-giving methods. Under compression, the stress pattern of the rubber bearing is three-dimensional and middle rubber bear the greatest force. Under tensile loading, the middle part of the rubber contract and the side with smaller lateral section has greater shrinkage; moreover, damage occurred in the area with stress concentration and weak strength. Under shearing action, extrude faces appeared with crinkle and damage occurred in the middle part of extrude faces. At the low temperature-40 , rubber support still has great elastic properties. The low temperature has a big effect on tensile properties and has little effect on damage properties.

Poly(N -isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three-dimensional stem cell culture and cartilage tissue engineering

2016 ◽  
Vol 104 (11) ◽  
pp. 2764-2774 ◽  
Author(s):  
Amir Mellati ◽  
Meisam Valizadeh Kiamahalleh ◽  
S. Hadi Madani ◽  
Sheng Dai ◽  
Jingxiu Bi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Stem Cell Culture ◽  
Chitosan Scaffold ◽  
And Cartilage

Impact response of a low-cost randomly oriented fiber foam core sandwich panel

2018 ◽  
Vol 52 (25) ◽  
pp. 3429-3444 ◽  
Author(s):  
Ezequiel Buenrostro ◽  
Daniel Whisler
Keyword(s):  
Mechanical Properties ◽  
Sandwich Panel ◽  
Low Cost ◽  
Three Dimensional ◽  
Cost Effective ◽  
Foam Core ◽  
Fiber Reinforced ◽  
Manufacturing Method ◽  
Manufacturing Techniques ◽  
The Impact

Three-dimensional fiber-reinforced foam cores may have improved mechanical properties under specific strain rates and fiber volumes. But its performance as a core in a composite sandwich structure has not been fully investigated. This study explored different manufacturing techniques for the three-dimensional fiber-reinforced foam core using existing literature as a guideline to provide a proof of concept for a low-cost and easily repeatable method comprised of readily available materials. The mechanical properties of the fiber-reinforced foam were determined using a three-point bend test and compared to unreinforced polyurethane foam. The foam was then used in a sandwich panel and subjected to dynamic loading by means of a gas gun (103 s−1). High-strain impact tests validated previously published studies by showing, qualitatively and quantitatively, an 18–20% reduction in the maximum force experienced by the fiber-reinforced core and its ability to dissipate the impact force in the foam core sandwich panel. The results show potential for this cost-effective manufacturing method to produce an improved composite foam core sandwich panel for applications where high-velocity impacts are probable. This has the potential to reduce manufacturing and operating costs while improving performance.

scholarly journals Three-Dimensional Bioprinting of Cartilage by the Use of Stem Cells: A Strategy to Improve Regeneration

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1749 ◽  
Author(s):  
Livia Roseti ◽  
Carola Cavallo ◽  
Giovanna Desando ◽  
Valentina Parisi ◽  
Mauro Petretta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Life Quality ◽  
Chemical Properties ◽  
Cartilage Tissue ◽  
Layer By Layer ◽  
Search Terms ◽  
Layer Deposition ◽  
Fine Control

Cartilage lesions fail to heal spontaneously, leading to the development of chronic conditions which worsen the life quality of patients. Three-dimensional scaffold-based bioprinting holds the potential of tissue regeneration through the creation of organized, living constructs via a “layer-by-layer” deposition of small units of biomaterials and cells. This technique displays important advantages to mimic natural cartilage over traditional methods by allowing a fine control of cell distribution, and the modulation of mechanical and chemical properties. This opens up a number of new perspectives including personalized medicine through the development of complex structures (the osteochondral compartment), different types of cartilage (hyaline, fibrous), and constructs according to a specific patient’s needs. However, the choice of the ideal combination of biomaterials and cells for cartilage bioprinting is still a challenge. Stem cells may improve material mimicry ability thanks to their unique properties: the immune-privileged status and the paracrine activity. Here, we review the recent advances in cartilage three-dimensional, scaffold-based bioprinting using stem cells and identify future developments for clinical translation. Database search terms used to write this review were: “articular cartilage”, “menisci”, “3D bioprinting”, “bioinks”, “stem cells”, and “cartilage tissue engineering”.

scholarly journals Biosafety evaluation of culture-expanded human chondrocytes with growth factor cocktail: a preclinical study

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maimonah-Eissa Al-Masawa ◽  
Wan Safwani Wan Kamarul Zaman ◽  
Kien-Hui Chua
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Preclinical Study ◽  
Culture Conditions ◽  
Cartilage Tissue ◽  
Tumour Suppressor Genes ◽  
Population Doublings ◽  
The Impact

AbstractThe scarcity of chondrocytes is a major challenge for cartilage tissue engineering. Monolayer expansion is necessary to amplify the limited number of chondrocytes needed for clinical application. Growth factors are often added to improve monolayer culture conditions, promoting proliferation, and enhancing chondrogenesis. Limited knowledge on the biosafety of the cell products manipulated with growth factors in culture has driven this study to evaluate the impact of growth factor cocktail supplements in chondrocyte culture medium on chondrocyte genetic stability and tumorigenicity. The growth factors were basic fibroblast growth factor (b-FGF), transforming growth factor β2 (TGF β2), insulin-like growth factor 1 (IGF-1), insulin-transferrin-selenium (ITS), and platelet-derived growth factor (PD-GF). Nasal septal chondrocytes cultured in growth factor cocktail exhibited a significantly high proliferative capacity. Comet assay revealed no significant DNA damage. Flow cytometry showed chondrocytes were mostly at G0-G1 phase, exhibiting normal cell cycle profile with no aneuploidy. We observed a decreased tumour suppressor genes’ expression (p53, p21, pRB) and no TP53 mutations or tumour formation after 6 months of implantation in nude mice. Our data suggest growth factor cocktail has a low risk of inducing genotoxic and tumorigenic effects on chondrocytes up to passage 6 with 16.6 population doublings. This preclinical tumorigenicity and genetic instability evaluation is crucial for further clinical works.

Three-Dimensional Porous Scaffolds with Biomimetic Microarchitecture and Bioactivity for Cartilage Tissue Engineering

2019 ◽  
Vol 11 (40) ◽  
pp. 36359-36370 ◽  
Author(s):  
Yaqiang Li ◽  
Yanqun Liu ◽  
Xiaowei Xun ◽  
Wei Zhang ◽  
Yong Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Porous Scaffolds

scholarly journals Mechanical loading inhibits hypertrophy in chondrogenically differentiating hMSCs within a biomimetic hydrogel

2016 ◽  
Vol 4 (20) ◽  
pp. 3562-3574 ◽  
Author(s):  
E. A. Aisenbrey ◽  
S. J. Bryant
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mechanical Loading ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cartilage Tissue

Three dimensional hydrogels are a promising vehicle for delivery of adult human bone-marrow derived mesenchymal stem cells (hMSCs) for cartilage tissue engineering.

scholarly journals Three-Dimensional Printing Strategies for Irregularly Shaped Cartilage Tissue Engineering: Current State and Challenges

2022 ◽  
Vol 9 ◽  
Author(s):  
Hui Wang ◽  
Zhonghan Wang ◽  
He Liu ◽  
Jiaqi Liu ◽  
Ronghang Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Future Research ◽  
Engineering Construction ◽  
Three Dimensional Printing ◽  
Current State ◽  
Dimensional Printing

Although there have been remarkable advances in cartilage tissue engineering, construction of irregularly shaped cartilage, including auricular, nasal, tracheal, and meniscus cartilages, remains challenging because of the difficulty in reproducing its precise structure and specific function. Among the advanced fabrication methods, three-dimensional (3D) printing technology offers great potential for achieving shape imitation and bionic performance in cartilage tissue engineering. This review discusses requirements for 3D printing of various irregularly shaped cartilage tissues, as well as selection of appropriate printing materials and seed cells. Current advances in 3D printing of irregularly shaped cartilage are also highlighted. Finally, developments in various types of cartilage tissue are described. This review is intended to provide guidance for future research in tissue engineering of irregularly shaped cartilage.

Sign in / Sign up

Export Citation Format

Share Document