Influence of storage conditions on the LIDT of very high damage resistance mirrors

A microbial profile of smoked dry herring stored under various storage conditions for up to 6 months at ambient temperature was determined. The fish were stored in polyethylene bags, polyethylene bags with desiccant, the traditional oven storage, modified storage structure, and freezer (control) storage. The fish stored in polyethylene bags with or without desiccant spoiled rapidly. Microbial counts were also very high. The microbial counts of fish stored in the traditional oven and the modified structure were small, even less than that stored in freezer. The storage losses were only 4% for the modified structure while the traditional oven storage was 29%. The major pathogenic bacterium found in smoked dry herring was Staphylococcus aureus.

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Robotic and Automated Systems in the Pharmaceutical QC

JALA Journal of the Association for Laboratory Automation ◽

10.1016/s1535-5535-04-00047-4 ◽

2000 ◽

Vol 5 (1) ◽

pp. 40-43 ◽

Cited By ~ 2

Author(s):

Franz Zick ◽

Manfred Fischer ◽

Byk Gulden

Keyword(s):

Storage Conditions ◽

Test Methods ◽

Stability Study ◽

Robotic Systems ◽

Automated Systems ◽

Method Transfer ◽

Enteric Coated ◽

Manual Testing ◽

Release Testing ◽

Very High

Since the beginning of this decade the use of automated or robotic-systems for analytical purposes has decreased rapidely. Starting from the analysis of agriculturing products or medical samples, these instruments are now more and more common in the pharmaceutical industry. The systems are used for R&D purposes mainly in the area of combinatorial chemistry to analyze the huge amount of synthesis products in the biomedical labs and in chemical-physical laboratories of Quality Contol Units. Extensive stability studies, which have to be perfomed for new drug applications, and the release testing of medicinal products with a very high manufacturing batch-rate per year need highly effective analytical methods. Thus, Byk Gulden has introduced two automated systems based on Zymark products (TPWII and MultiDose), for the stability tests of an enteric-coated tablet. The number of batches, pack types and storage conditions (total number of samples: appr. 40 for the 3- and 6-months timepoints), for this stability study was required automation of the most time consuming test methods for purity, assay and dissolution of the tablets, because of the tight schedule for timepoints in the first year of storage (in general: two weeks). In order to have these automated systems in place for routine testing in a very short time, the method transfer from the manual procedure as well as the optimization and validation work (MTOV), were done completely by the supplier, Zymark. The other system, which is a customer designed robotic system built also by Zymark, is used for the release testing of an x-ray contrast medium with a very high batch-rate per year (approxmimately four batches per day that have to be analyzed). This enormous testing frequency is hardly to be realized by manual methods. Experiences with an already existent robotic systems has shown that the laboratory flow-through times are 30 to 50% decreased compared to manual testing.

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DoE-Assisted Development of a Novel Glycosaminoglycan-Based Injectable Formulation for Viscosupplementation

Pharmaceutics ◽

10.3390/pharmaceutics12070681 ◽

2020 ◽

Vol 12 (7) ◽

pp. 681

Author(s):

Marta Cicognani ◽

Silvia Rossi ◽

Gabriele Vecchi ◽

Andrea Maria Giori ◽

Franca Ferrari

Keyword(s):

Synovial Fluid ◽

Viscoelastic Properties ◽

Pilot Scale ◽

Storage Conditions ◽

International Council ◽

Injectable Formulation ◽

Full Factorial ◽

Very High ◽

Lubrication Properties

The aim of the present work was the development of a novel glycosaminoglycan (GAG)-based injectable formulation intended for intra-articular administration that should best mimic the healthy synovial fluid. Hyaluronic acid (HA) was chosen among GAG polymers, since it is the most abundant component of the synovial fluid. A DoE (Design of Experiment) approach was used for the development of a formulation containing two HA (very high (VHMW) and low (LMW) molecular weight) grades. The rationale for this choice is that so far, no commercial product based on a single HA grade or even on binary HA mixture possesses optimal viscoelastic properties in comparison with healthy synovial fluid. A full factorial design was chosen to investigate the influence of concentration and relative fraction of the two polymer grades (retained as factors of the model) on formulation functional (viscosity and viscoelastic) properties, which are considered response variables. Thanks to the DoE approach, the composition of the optimized HA formulation was found. The addition to such formulation of an injectable grade fat-free soy phospholipid, which was rich in phosphatidylcholine (PC), resulted in improved lubrication properties. The final HA + PC formulation, packaged in pre-filled sterile syringes, was stable in long-term and accelerated ICH (International Council for Harmonisation) storage conditions. The overall results pointed out the formulation suitability for further steps of pharmaceutical developments, namely for the passage to pilot scale.

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Use of faecal pollution indicators to estimate pathogen die off conditions in source separated faeces in Kathmandu Valley, Nepal

Journal of Water and Health ◽

10.2166/wh.2009.149 ◽

2008 ◽

Vol 7 (1) ◽

pp. 97-107 ◽

Cited By ~ 8

Author(s):

Anjali Manandhar Sherpa ◽

Denis Byamukama ◽

Roshan R. Shrestha ◽

Raimund Haberl ◽

Robert L. Mach ◽

...

Keyword(s):

Storage Time ◽

Reduction Rate ◽

Storage Conditions ◽

Kathmandu Valley ◽

Chemical Parameters ◽

E Coli ◽

Phosphorous Content ◽

Physical And Chemical ◽

Very High ◽

Faecal Indicators

As the introduction and promotion of dehydrating toilets progresses, the safety of handling and reuse of their biosolids remains a question. A detailed study to understand the storage conditions and the fate of selected faecal indicators was conducted on four urine diverting dehydrating toilet units, using ash as a major additive, in Kathmandu Valley, Nepal. Presumptive Escherichia coli, total coliforms, enterococci and different fractions of Clostridium perfringens were investigated under field storage conditions. In addition, chemo-physical and chemical (carbon, nitrogen, phosphorous content) parameters were investigated. Observed temperature was low in all the four toilets with a median of 24.0°C, which was in the same range as the ambient temperature. pH was below the desired range of >9 and moisture level was very high (>60%). No single factor of the studied chemo-physical and chemical parameters could be found by statistical analysis to have accounted for the reduction of the indicators in any of the toilets. By time series analysis of the investigated strata in the faecal heaps (n=96), the determined reduction rate showed increasing persistence characteristics for E. coli, coliforms and enterococci with respective average log10 reduction of −0.4, −0.3 and −0.2 per month (p<0.001). No significant reduction was observed for the different fractions of C. perfringens determined for the non-pasteurised and pasteurised fraction at 60°C and 85°C. 72% of randomly selected and analysed samples (n=36) were found to contain helminthes eggs. The used 6 months storage time did not prove sufficient to reach appropriate safety levels for handling and reuse of the biosolids.

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Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.581148 ◽

2021 ◽

Vol 8 ◽

Author(s):

Julia Ramos Sampaio ◽

Rodrigo Pedro Soares ◽

Thiago Doria Barral ◽

Gabriela Porfirio Passos ◽

Maisa Santos Fonseca ◽

...

Keyword(s):

Leishmania Infantum ◽

Point Of Care ◽

High Specificity ◽

Storage Conditions ◽

Canine Leishmaniasis ◽

Lower Sensitivity ◽

Immune Recognition ◽

Specificity And Sensitivity ◽

Lower Specificity ◽

Very High

Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative.

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Effect of tomato post-harvest treatment and storage conditions on the biological activity of benomyl and methylthiophanate residues in fruits and their processed products

Acta Agrobotanica ◽

10.5586/aa.1982.027 ◽

2013 ◽

Vol 35 (2) ◽

pp. 269-275

Author(s):

H. Borecka ◽

H. Parynow

Keyword(s):

Biological Activity ◽

Storage Period ◽

Low Pressure ◽

Storage Conditions ◽

Post Harvest ◽

Fungicide Residues ◽

Processed Products ◽

And Storage ◽

Very High

The biological activity of benomyl and methylthiophanate residues in tomatoes and their processed products was examined. Fungicide residues were most active in concentrated pulp of tomatoes, less in pulp and the least in fresh fruits. The fungicide residues activity was very high after 90 days of storage of processed products. The pulp and concentrated pulp of tomatoes treated and stored for 14 days at 15°C, also contained very active fungicide residues. The translocation of benomyl from treated tomato skin into the tissue begun slowly. This process was faster in green or yellow fruits, but it was the fastest in small green fruits. The influence of storage conditions on the activity of fungicide residues depended on the storage length. The residues were the least active in fruits stored under low pressure of 38 mm Hg for 14 days, but this activity increased during the storage period and the residues were very active after 34 days of tomato storage.

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Long-term performances of very high-laser-damage resistance mirrors

10.1117/12.472401 ◽

2003 ◽

Cited By ~ 2

Author(s):

Guillaume Ravel ◽

Philippe Bouchut ◽

Pierre Garrec ◽

Bernard Andre ◽

Carol Le Diraison ◽

...

Keyword(s):

Laser Damage ◽

Damage Resistance ◽

High Laser ◽

Very High

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Very High Resolution Analysis of the Dynamics of a Coronal Plasmoid

International Astronomical Union Colloquium ◽

10.1017/s0252921100026117 ◽

1994 ◽

Vol 144 ◽

pp. 593-596

Author(s):

O. Bouchard ◽

S. Koutchmy ◽

L. November ◽

J.-C. Vial ◽

J. B. Zirker

Keyword(s):

High Resolution ◽

Solar Eclipse ◽

Total Solar Eclipse ◽

Field Of View ◽

Small Field ◽

High Resolution Analysis ◽

Ccd Observations ◽

Resolution Analysis ◽

Very High ◽

Small Field Of View

AbstractWe present the results of the analysis of a movie taken over a small field of view in the intermediate corona at a spatial resolution of 0.5“, a temporal resolution of 1 s and a spectral passband of 7 nm. These CCD observations were made at the prime focus of the 3.6 m aperture CFHT telescope during the 1991 total solar eclipse.

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Monte Carlo Algorithms for Moments of Transition Arrays

International Astronomical Union Colloquium ◽

10.1017/s0252921100107468 ◽

1988 ◽

Vol 102 ◽

pp. 79-81

Author(s):

A. Goldberg ◽

S.D. Bloom

Keyword(s):

Monte Carlo ◽

State Vector ◽

Basis Vector ◽

Collective State ◽

Dispersion Characteristics ◽

Dipole Strength ◽

The Third ◽

Monte Carlo Algorithms ◽

Third Moment ◽

Very High

AbstractClosed expressions for the first, second, and (in some cases) the third moment of atomic transition arrays now exist. Recently a method has been developed for getting to very high moments (up to the 12th and beyond) in cases where a “collective” state-vector (i.e. a state-vector containing the entire electric dipole strength) can be created from each eigenstate in the parent configuration. Both of these approaches give exact results. Herein we describe astatistical(or Monte Carlo) approach which requires onlyonerepresentative state-vector |RV> for the entire parent manifold to get estimates of transition moments of high order. The representation is achieved through the random amplitudes associated with each basis vector making up |RV>. This also gives rise to the dispersion characterizing the method, which has been applied to a system (in the M shell) with≈250,000 lines where we have calculated up to the 5th moment. It turns out that the dispersion in the moments decreases with the size of the manifold, making its application to very big systems statistically advantageous. A discussion of the method and these dispersion characteristics will be presented.

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Decomposition of the metastable beta titanium alloy Ti-15-3

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075117 ◽

1983 ◽

Vol 41 ◽

pp. 264-265

Author(s):

Y. L. Chen ◽

S. Fujlshiro

Keyword(s):

Low Cost ◽

High Strength ◽

Alpha Phase ◽

Thick Sheet ◽

Omega Phase ◽

Beta Titanium Alloy ◽

Beta Titanium ◽

Beta Matrix ◽

Metastable Beta ◽

Very High

Metastable beta titanium alloys have been known to have numerous advantages such as cold formability, high strength, good fracture resistance, deep hardenability, and cost effectiveness. Very high strength is obtainable by precipitation of the hexagonal alpha phase in a bcc beta matrix in these alloys. Precipitation hardening in the metastable beta alloys may also result from the formation of transition phases such as omega phase. Ti-15-3 (Ti-15V- 3Cr-3Al-3Sn) has been developed recently by TIMET and USAF for low cost sheet metal applications. The purpose of the present study was to examine the aging characteristics in this alloy.The composition of the as-received material is: 14.7 V, 3.14 Cr, 3.05 Al, 2.26 Sn, and 0.145 Fe. The beta transus temperature as determined by optical metallographic method was about 770°C. Specimen coupons were prepared from a mill-annealed 1.2 mm thick sheet, and solution treated at 827°C for 2 hr in argon, then water quenched. Aging was also done in argon at temperatures ranging from 316 to 616°C for various times.

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