scholarly journals Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration

2018 ◽  
Vol 4 (1) ◽  
pp. e1601453 ◽  
Author(s):  
Kyoungwon Park ◽  
Yung Kuo ◽  
Volodymyr Shvadchak ◽  
Antonino Ingargiola ◽  
Xinghong Dai ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Membrane Insertion ◽  
Semiconductor Voltage

scholarly journals Membrane insertion of‐ and membrane potential sensing by semiconductor voltage nanosensors: feasibility demonstration

2016 ◽  
Author(s):  
Kyoungwon Park ◽  
Yung Kuo ◽  
Volodymyr Shvadchak ◽  
Antonino Ingargiola ◽  
Xinghong Dai ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Stark Effect ◽  
Semiconductor Nanoparticles ◽  
Field Of View ◽  
Large Field ◽  
Membrane Insertion ◽  
Quantum Confined Stark Effect ◽  
Long Duration ◽  
Semiconductor Voltage ◽  
Inorganic Semiconductor

AbstractWe develop membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. These voltage nanosensors are designed to self-insert into the cell membrane and optically record the membrane potential via the quantum confined Stark effect, with single-particle sensitivity. We present here the approach, design rules, and feasibility proves for this concept. With further improvements, semiconductor nanoparticles could potentially be used to study signals from many neurons in a large field-of-view over a long duration. Moreover, they could potentially report and resolve voltage signals on the nanoscale.

scholarly journals Feasibility analysis of semiconductor voltage nanosensors for neuronal membrane potential sensing

2019 ◽  
Author(s):  
Anastasia Ludwig ◽  
Pablo Serna ◽  
Lion Morgenstein ◽  
Gaoling Yang ◽  
Omri Bar-Elli ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Electric Fields ◽  
Stark Effect ◽  
High Sensitivity ◽  
Time Lapse ◽  
Neuronal Membrane ◽  
Attractive Alternative ◽  
Wide Field ◽  
Particle Level ◽  
Semiconductor Voltage

AbstractIn the last decade, optical imaging methods have significantly improved our understanding of the information processing principles in the brain. Although many promising tools have been designed, sensors of membrane potential are lagging behind the rest. Semiconductor nanoparticles are an attractive alternative to classical voltage indicators, such as voltage-sensitive dyes and proteins. Such nanoparticles exhibit high sensitivity to external electric fields via the quantum-confined Stark effect. Here we report the development of lipid-coated semiconductor voltage-sensitive nanorods (vsNRs) that self-insert into the neuronal membrane. We describe a workflow to detect and process the photoluminescent signal of vsNRs after wide-field time-lapse recordings. We also present data indicating that vsNRs are feasible for sensing membrane potential in neurons at a single-particle level. This shows the potential of vsNRs for detection of neuronal activity with unprecedentedly high spatial and temporal resolution.

scholarly journals Repolarization of the membrane potential of blood platelets after complement damage: evidence for a Ca++ -dependent exocytotic elimination of C5b-9 pores

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 556-561 ◽  
Author(s):  
PJ Sims ◽  
T Wiedmer
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Serum Proteins ◽  
Blood Platelets ◽  
Membrane Damage ◽  
Membrane Insertion ◽  
Ion Conductance ◽  
Platelet Surface ◽  
Membrane Pore ◽  
Membrane Rupture

Abstract Gel-filtered blood platelets exposed to complement proteins C5b-9 have previously been shown to undergo a reversible depolarization of membrane potential (Em) in the absence of lytic plasma membrane rupture. In this paper, we examine the mechanism by which C5b-9 damaged platelets restore their basal electrochemical state, despite increased ion conductance due to membrane insertion of these cytolytic serum proteins. Repolarization of Em after formation of the C5b-9 membrane pore is shown to be accompanied by a Ca++-dependent vesiculation of the platelet surface, which results in the release of these proteins from the plasma membrane and a restoration of the membrane's functional integrity. This exocytotic elimination of C5b-9 complexes from the plasma membrane is accompanied by a ouabain-inhibitable repolarization of Em, which presumably reflects restoration of transmembrane cation gradients by the plasma membrane Na/K ATPase. The role of external Ca++ in the platelet's response to membrane-insertion of the C5b-9 proteins is discussed both in the context of the known cellular effects of this ion and in the context of recent observations suggesting sublytic changes in platelet function after complement-mediated plasma membrane damage.

scholarly journals Repolarization of the membrane potential of blood platelets after complement damage: evidence for a Ca++ -dependent exocytotic elimination of C5b-9 pores

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 556-561 ◽  
Author(s):  
PJ Sims ◽  
T Wiedmer
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Serum Proteins ◽  
Blood Platelets ◽  
Membrane Damage ◽  
Membrane Insertion ◽  
Ion Conductance ◽  
Platelet Surface ◽  
Membrane Pore ◽  
Membrane Rupture

Gel-filtered blood platelets exposed to complement proteins C5b-9 have previously been shown to undergo a reversible depolarization of membrane potential (Em) in the absence of lytic plasma membrane rupture. In this paper, we examine the mechanism by which C5b-9 damaged platelets restore their basal electrochemical state, despite increased ion conductance due to membrane insertion of these cytolytic serum proteins. Repolarization of Em after formation of the C5b-9 membrane pore is shown to be accompanied by a Ca++-dependent vesiculation of the platelet surface, which results in the release of these proteins from the plasma membrane and a restoration of the membrane's functional integrity. This exocytotic elimination of C5b-9 complexes from the plasma membrane is accompanied by a ouabain-inhibitable repolarization of Em, which presumably reflects restoration of transmembrane cation gradients by the plasma membrane Na/K ATPase. The role of external Ca++ in the platelet's response to membrane-insertion of the C5b-9 proteins is discussed both in the context of the known cellular effects of this ion and in the context of recent observations suggesting sublytic changes in platelet function after complement-mediated plasma membrane damage.

scholarly journals An amino acid sequence which directs membrane insertion causes loss of membrane potential.

1988 ◽  
Vol 263 (23) ◽  
pp. 11575-11583 ◽  
Author(s):  
J I Horabin ◽  
R E Webster
Keyword(s):  
Membrane Potential ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Membrane Insertion

Measurement of spontaneous neuronal membrane activity by time lapse confocal microscopy

1995 ◽  
Vol 53 ◽  
pp. 972-973
Author(s):  
R H. Selinfreund ◽  
A. H. Cornell-Bell
Keyword(s):  
Membrane Potential ◽  
Confocal Microscopy ◽  
Patch Clamp ◽  
Electrical Activity ◽  
Time Lapse ◽  
Neuronal Firing ◽  
Neuronal Membrane ◽  
Pituitary Cells ◽  
Patch Clamp Recording ◽  
Spontaneous Electrical Activity

Cellular electrophysiological properties are normally monitored by standard patch clamp techniques . The combination of membrane potential dyes with time-lapse laser confocal microscopy provides a more direct, least destructive rapid method for monitoring changes in neuronal electrical activity. Using membrane potential dyes we found that spontaneous action potential firing can be detected using time-lapse confocal microscopy. Initially, patch clamp recording techniques were used to verify spontaneous electrical activity in GH4\C1 pituitary cells. It was found that serum depleted cells had reduced spontaneous electrical activity. Brief exposure to the serum derived growth factor, IGF-1, reconstituted electrical activity. We have examined the possibility of developing a rapid fluorescent assay to measure neuronal activity using membrane potential dyes. This neuronal regeneration assay has been adapted to run on a confocal microscope. Quantitative fluorescence is then used to measure a compounds ability to regenerate neuronal firing.The membrane potential dye di-8-ANEPPS was selected for these experiments. Di-8- ANEPPS is internalized slowly, has a high signal to noise ratio (40:1), has a linear fluorescent response to change in voltage.

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

Ca2+ Accumulation in Isolated Rat Heart Mitochondria under Maintenance of Mitochondrial Membrane Potential

2016 ◽  
Vol 7 (4) ◽  
pp. 309-319
Author(s):  
Alina Yu. Budko ◽  
Nataliya A. Strutynska ◽  
Iryna Yu. Okhay ◽  
Olena M. Semenykhina ◽  
Vadim F. Sagach
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Rat Heart ◽  
Mitochondrial Membrane ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Isolated Rat
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