Cryo-shocked cancer cells for targeted drug delivery and vaccination

Live cells have been vastly engineered into drug delivery vehicles to leverage their targeting capability and cargo release behavior. Here, we describe a simple method to obtain therapeutics-containing “dead cells” by shocking live cancer cells in liquid nitrogen to eliminate pathogenicity while preserving their major structure and chemotaxis toward the lesion site. In an acute myeloid leukemia (AML) mouse model, we demonstrated that the liquid nitrogen–treated AML cells (LNT cells) can augment targeted delivery of doxorubicin (DOX) toward the bone marrow. Moreover, LNT cells serve as a cancer vaccine and promote antitumor immune responses that prolong the survival of tumor-bearing mice. Preimmunization with LNT cells along with an adjuvant also protected healthy mice from AML cell challenge.

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Well-defined single polymer nanoparticles for the antibody-targeted delivery of chemotherapeutic agents

Polymer Chemistry ◽

10.1039/c4py01250j ◽

2015 ◽

Vol 6 (8) ◽

pp. 1286-1299 ◽

Cited By ~ 13

Author(s):

D. D. Lane ◽

D. Y. Chiu ◽

F. Y. Su ◽

S. Srinivasan ◽

H. B. Kern ◽

...

Keyword(s):

Drug Delivery ◽

Targeted Drug Delivery ◽

Targeted Delivery ◽

Raft Polymerization ◽

Chemotherapeutic Agents ◽

Polymer Nanoparticles ◽

Drug Delivery Vehicles ◽

Molecular Weights ◽

Delivery Vehicles ◽

Targeted Drug

Second generation polymeric brushes with molecular weights in excess of 106 Da were synthesize via RAFT polymerization for use as antibody targeted drug delivery vehicles.

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Targeted Drug Delivery in Lipid-like Nanocages and Extracellular Vesicles

Acta Naturae ◽

10.32607/20758251-2019-11-2-28-41 ◽

2019 ◽

Vol 11 (2) ◽

pp. 28-41 ◽

Cited By ~ 2

Author(s):

A. V. Sokolov ◽

N. N. Kostin ◽

L. A. Ovchinnikova ◽

Y. A. Lomakin ◽

A. A. Kudriaeva

Keyword(s):

Drug Delivery ◽

Extracellular Vesicles ◽

Targeted Drug Delivery ◽

Targeted Delivery ◽

Structural Similarity ◽

Biological Properties ◽

Drug Delivery Vehicles ◽

Delivery Vehicles ◽

Targeted Drug ◽

High Level

The possibility of targeted drug delivery to a specific tissue, organ, or cell has opened new promising avenues in treatment development. The technology of targeted delivery aims to create multifunctional carriers that are capable of long circulation in the patients organism and possess low toxicity at the same time. The surface of modern synthetic carriers has high structural similarity to the cell membrane, which, when combined with additional modifications, also promotes the transfer of biological properties in order to penetrate physiological barriers effectively. Along with artificial nanocages, further efforts have recently been devoted to research into extracellular vesicles that could serve as natural drug delivery vehicles. This review provides a detailed description of targeted delivery systems that employ lipid and lipid-like nanocages, as well as extracellular vesicles with a high level of biocompatibility, highlighting genetically encoded drug delivery vehicles.

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Tunable immunonanoparticle binding to cancer cells: thermodynamic analysis of targeted drug delivery vehicles

Soft Matter ◽

10.1039/b814204a ◽

2009 ◽

Vol 5 (5) ◽

pp. 1074 ◽

Cited By ~ 23

Author(s):

Karyn Ho ◽

Yakov Lapitsky ◽

Meng Shi ◽

Molly S. Shoichet

Keyword(s):

Drug Delivery ◽

Cancer Cells ◽

Thermodynamic Analysis ◽

Targeted Drug Delivery ◽

Drug Delivery Vehicles ◽

Delivery Vehicles ◽

Targeted Drug

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Active targeting of cancer cells using gemcitabine conjugated platinum nanoparticles

10.51415/10321/2642 ◽

2017 ◽

Author(s):

◽

Kriya Odayar

Keyword(s):

Drug Delivery ◽

Cell Death ◽

Cancer Cells ◽

Cell Line ◽

Platinum Nanoparticles ◽

Targeted Drug Delivery ◽

Caspase 3 ◽

Drug Delivery Vehicles ◽

Delivery Vehicles ◽

Targeted Drug

Nanotechnology is explained as the science of engineered materials and systems on a molecular scale. This innovation is currently used in a wide variety of applications which include using these nanoparticles as drug delivery vehicles. Such nanocarriers are relatively smaller than 100 nm in size with the ability to convey therapeutic drugs to a number of disease sites. Platinum-based nanoparticles have been extensively used in a number of applications namely catalysts, gas sensors, glucose sensors and cancer therapy. The properties of platinum nanoparticles (PtNP’s) typically depend on characteristics such as shape, particle size, elemental composition and structure, all of which can be manipulated and controlled in the fabrication process. Their unique size in the nanometer scale makes platinum nanoparticles an ideal candidate as targeted drug delivery vehicles. To target an anticancer drug to a diseased site is a distinctive feature of most studies, which aim to transfer an adequate dosage of the drug to cancer cells. Transport systems used as carriers of anticancer drugs offer numerous advantages, which include improved efficacy and a decrease in toxicity towards healthy cells when compared to standard drugs. The aim of this study was to determine the effect of platinum nanoparticles, gemcitabine and gemcitabine conjugated platinum nanoparticles (Hybrids) against cancer cells and healthy cells and to determine the mode of cell death and cell death pathways using flow cytometry. Platinum nanoparticles were synthesized via the reduction of hexachloroplatinic acid using sodium borohydride in the presence of capping agents. Synthesized platinum nanoparticles and the hybrids were characterized by observing peaks at 301 nm and 379 nm respectively using UV-visible spectroscopy. TEM images revealed that the PtNP’s and the conjugate compounds were spherical in shape with core sizes of 1.14 nm - 1.65 nm and 1.53 - 2.66 nm respectively. The bioactivity platinum nanoparticles, gemcitabine and the hybrids were investigated using MCF7 and Melanoma cancer cells at different concentrations from 0.10 to 100 µg/ml. Results indicated that conjugated nanoparticles induced the highest cell inhibition against both cell lines compared to gemcitabine and platinum nanoparticles. Bioactivity against PBMC (peripheral blood mononuclear) cells indicated that all three compounds show little or no effect towards the healthy cell line compared to the control. Melanoma cell line was used to determine the mode of cell death. Apoptosis was detected using Annexin V-FITC to detect membrane changes, JC-1 to detect a loss in mitochondrial membrane potential and caspase-3 assay kits. Results indicated that a significant amount of cell death was caused by cleavage of caspase-3. Nanoparticle drug delivery is an area that has shown significant promise in cancer treatment. Interaction of nanoparticles with human cells is an interesting topic for understanding toxicity and developing potential drug candidates. Imagine, something that is atleast or more than 80,000 times smaller than the edge of the ridge on a fingertip and unlocks a new wilderness into cancer research. Nanotechnology, known as the science of minute, is changing the approach to cancer and especially future diagnosis and treatment. Nanotechnology permits scientists to fabricate new apparatuses that are definitely smaller than cells, giving them the chance to attack tumor diseased cells. This innovation not just empowers practitioners to recognize malignancies prior but additionally holds the guarantee of halting cancer growth before it further develops. This progressive approach is so exact, specialists will in future be able to outline a unique treatment for an individual’s own restorative and hereditary profile. Researchers are designing nanoparticles that detect and destroy diseased cells and this optimistic innovation could be personalized for targeted drug delivery, enhanced imaging and ongoing affirmation of cancer cell death. The National Cancer Institute remains hopeful that facilitated development, nanotechnology will drastically change cancer treatment.

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Functionalization of Reduced Graphene Oxide via Thiol–Maleimide “Click” Chemistry: Facile Fabrication of Targeted Drug Delivery Vehicles

ACS Applied Materials & Interfaces ◽

10.1021/acsami.7b08433 ◽

2017 ◽

Vol 9 (39) ◽

pp. 34194-34203 ◽

Cited By ~ 24

Author(s):

Yavuz Oz ◽

Alexandre Barras ◽

Rana Sanyal ◽

Rabah Boukherroub ◽

Sabine Szunerits ◽

...

Keyword(s):

Drug Delivery ◽

Graphene Oxide ◽

Click Chemistry ◽

Reduced Graphene Oxide ◽

Targeted Drug Delivery ◽

Drug Delivery Vehicles ◽

Reduced Graphene ◽

Facile Fabrication ◽

Delivery Vehicles ◽

Targeted Drug

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Development of novel PLGA contrast agents for use as ultrasound targeted drug delivery vehicles

10.17918/etd-182 ◽

2021 ◽

Author(s):

Dalia El-Sherif

Keyword(s):

Drug Delivery ◽

Contrast Agents ◽

Targeted Drug Delivery ◽

Drug Delivery Vehicles ◽

Delivery Vehicles ◽

Targeted Drug

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Nano-Biomimetic Drug Delivery Vehicles: Potential Approaches for COVID-19 Treatment

Molecules ◽

10.3390/molecules25245952 ◽

2020 ◽

Vol 25 (24) ◽

pp. 5952

Author(s):

Bwalya A. Witika ◽

Pedzisai A. Makoni ◽

Larry L. Mweetwa ◽

Pascal V. Ntemi ◽

Melissa T. R. Chikukwa ◽

...

Keyword(s):

Drug Delivery ◽

Targeted Delivery ◽

Inflammatory Diseases ◽

Cell Types ◽

Charge Composition ◽

Drug Delivery Vehicles ◽

Therapeutic Outcomes ◽

Delivery Vehicles ◽

The Impact

The current COVID-19 pandemic has tested the resolve of the global community with more than 35 million infections worldwide and numbers increasing with no cure or vaccine available to date. Nanomedicines have an advantage of providing enhanced permeability and retention and have been extensively studied as targeted drug delivery strategies for the treatment of different disease. The role of monocytes, erythrocytes, thrombocytes, and macrophages in diseases, including infectious and inflammatory diseases, cancer, and atherosclerosis, are better understood and have resulted in improved strategies for targeting and in some instances mimicking these cell types to improve therapeutic outcomes. Consequently, these primary cell types can be exploited for the purposes of serving as a “Trojan horse” for targeted delivery to identified organs and sites of inflammation. State of the art and potential utilization of nanocarriers such as nanospheres/nanocapsules, nanocrystals, liposomes, solid lipid nanoparticles/nano-structured lipid carriers, dendrimers, and nanosponges for biomimicry and/or targeted delivery of bioactives to cells are reported herein and their potential use in the treatment of COVID-19 infections discussed. Physicochemical properties, viz., hydrophilicity, particle shape, surface charge, composition, concentration, the use of different target-specific ligands on the surface of carriers, and the impact on carrier efficacy and specificity are also discussed.

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Facile synthesis of Prussian blue nanoparticles as pH-responsive drug carriers for combined photothermal-chemo treatment of cancer

RSC Advances ◽

10.1039/c6ra24979e ◽

2017 ◽

Vol 7 (1) ◽

pp. 248-255 ◽

Cited By ~ 26

Author(s):

Huajian Chen ◽

Yan Ma ◽

Xianwen Wang ◽

Xiaoyi Wu ◽

Zhengbao Zha

Keyword(s):

Drug Delivery ◽

Prussian Blue ◽

Cancer Cells ◽

Drug Carriers ◽

High Efficacy ◽

Ph Responsive ◽

Facile Synthesis ◽

Drug Delivery Vehicles ◽

Prussian Blue Nanoparticles ◽

Delivery Vehicles

Multifunctional PEGylated PB-DOX NPs with a lipid-PEG shell were developed as a gram-scale manner and used as novel pH-responsive drug delivery vehicles for combined photothermal-chemo treatment of cancer cells with high efficacy.

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