An engineered cell-laden adhesive hydrogel promotes craniofacial bone tissue regeneration in rats

2020 ◽  
Vol 12 (534) ◽  
pp. eaay6853 ◽  
Author(s):  
Mohammad Mahdi Hasani-Sadrabadi ◽  
Patricia Sarrion ◽  
Sevda Pouraghaei ◽  
Yee Chau ◽  
Sahar Ansari ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Craniofacial Bone ◽  
Oral Environment ◽  
Tunable Mechanical Properties ◽  
Craniofacial Tissue

Cell-laden hydrogels are widely used in tissue engineering and regenerative medicine. However, many of these hydrogels are not optimized for use in the oral environment, where they are exposed to blood and saliva. To address these challenges, we engineered an alginate-based adhesive, photocrosslinkable, and osteoconductive hydrogel biomaterial (AdhHG) with tunable mechanical properties. The engineered hydrogel was used as an injectable mesenchymal stem cell (MSC) delivery vehicle for craniofacial bone tissue engineering applications. Subcutaneous implantation in mice confirmed the biodegradability, biocompatibility, and osteoconductivity of the hydrogel. In a well-established rat peri-implantitis model, application of the adhesive hydrogel encapsulating gingival mesenchymal stem cells (GMSCs) resulted in complete bone regeneration around ailing dental implants with peri-implant bone loss. Together, we have developed a distinct bioinspired adhesive hydrogel with tunable mechanical properties and biodegradability that effectively delivers patient-derived dental-derived MSCs. The hydrogel is photocrosslinkable and, due to the presence of MSC aggregates and hydroxyapatite microparticles, promotes bone regeneration for craniofacial tissue engineering applications.

scholarly journals 3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1962 ◽  
Author(s):  
Abdullah M. Cakmak ◽  
Semra Unal ◽  
Ali Sahin ◽  
Faik N. Oktar ◽  
Mustafa Sengor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Bioactive Materials ◽  
Degradation Rates

Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.

PCL and PCL/PLA Scaffolds for Bone Tissue Regeneration

2013 ◽  
Vol 683 ◽  
pp. 168-171 ◽  
Author(s):  
Tatiana Patrício ◽  
Antonio Gloria ◽  
Paulo J. Bártolo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Cell Attachment ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Printing System

This paper investigates the use of PCL and PCL/PLA scaffolds, produced using a novel additive biomanufacturing system called BioCell Printing, for bone tissue engineering applications. Results show that the BioCell Printing system produces scaffolds with regular and reproducible architecture, presenting no toxicity and enhancing cell attachment and proliferation. It was also possible to observe that the addition of PLA to PCL scaffolds strongly improves the biomechanical performance of the constructs.

scholarly journals Bone Regeneration in Critical-Sized Bone Defects Treated with Additively Manufactured Porous Metallic Biomaterials: The Effects of Inelastic Mechanical Properties

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1992
Author(s):  
Marianne Koolen ◽  
Saber Amin Yavari ◽  
Karel Lietaert ◽  
Ruben Wauthle ◽  
Amir A. Zadpoor ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bulk Material ◽  
Bone Tissue Regeneration ◽  
Bony Tissue ◽  
Metallic Biomaterials ◽  
Cp Ti ◽  
Porous Biomaterials

Additively manufactured (AM) porous metallic biomaterials, in general, and AM porous titanium, in particular, have recently emerged as promising candidates for bone substitution. The porous design of such materials allows for mimicking the elastic mechanical properties of native bone tissue and showed to be effective in improving bone regeneration. It is, however, not clear what role the other mechanical properties of the bulk material such as ductility play in the performance of such biomaterials. In this study, we compared the bone tissue regeneration performance of AM porous biomaterials made from the commonly used titanium alloy Ti6Al4V-ELI with that of commercially pure titanium (CP-Ti). CP-Ti was selected because of its high ductility as compared to Ti6Al4V-ELI. Critical-sized (6 mm diameter) femoral defects in rats were treated with implants made from both Ti6Al4V-ELI and CP-Ti. Bone regeneration was assessed up to 11 weeks using micro-CT scanning. The regenerated bone volume was assessed ex vivo followed by histology and biomechanical testing to assess osseointegration of the implants. The bony defects treated with AM CP-Ti implants generally showed higher volumes of regenerated bone as compared to those treated with AM Ti6Al4V-ELI. The torsional strength of the two titanium groups were similar however, and both considerably lower than those measured for intact bony tissue. These findings show the importance of material type and ductility of the bulk material in the ability for bone tissue regeneration of AM porous biomaterials.

Fabrication of a chitosan/bioglass three-dimensional porous scaffold for bone tissue engineering applications

2014 ◽  
Vol 2 (38) ◽  
pp. 6611-6618 ◽  
Author(s):  
Jun Yang ◽  
Teng Long ◽  
Nan-Fei He ◽  
Ya-Ping Guo ◽  
Zhen-An Zhu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Bone Defects ◽  
Engineering Applications

A chitosan/bioglass three-dimensional porous scaffold with excellent biocompatibility and mechanical properties has been developed for the treatment of bone defects.

scholarly journals Is There a Governing Role of Osteocytes in Bone Tissue Regeneration?

2020 ◽  
Vol 18 (5) ◽  
pp. 541-550
Author(s):  
Wei Cao ◽  
Marco N. Helder ◽  
Nathalie Bravenboer ◽  
Gang Wu ◽  
Jianfeng Jin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Remodeling ◽  
Tissue Regeneration ◽  
Bone Cells ◽  
Bone Tissue Regeneration ◽  
Osteoblast Activity

Abstract Purpose of Review Bone regeneration plays an important role in contemporary clinical treatment. Bone tissue engineering should result in successful bone regeneration to restore congenital or acquired bone defects in the human skeleton. Osteocytes are thought to have a governing role in bone remodeling by regulating osteoclast and osteoblast activity, and thus bone loss and formation. In this review, we address the so far largely unknown role osteocytes may play in bone tissue regeneration. Recent Findings Osteocytes release biochemical signaling molecules involved in bone remodeling such as prostaglandins, nitric oxide, Wnts, and insulin-like growth factor-1 (IGF-1). Treatment of mesenchymal stem cells in bone tissue engineering with prostaglandins (e.g., PGE2, PGI2, PGF2α), nitric oxide, IGF-1, or Wnts (e.g., Wnt3a) improves osteogenesis. Summary This review provides an overview of the functions of osteocytes in bone tissue, their interaction with other bone cells, and their role in bone remodeling. We postulate that osteocytes may have a pivotal role in bone regeneration as well, and consequently that the bone regeneration process may be improved effectively and rapidly if osteocytes are optimally used and stimulated.

Nanomaterials for bone tissue regeneration: updates and future perspectives

Nanomedicine ◽  
2019 ◽  
Vol 14 (22) ◽  
pp. 2987-3006 ◽  
Author(s):  
Michael J Hill ◽  
Baowen Qi ◽  
Rasoul Bayaniahangar ◽  
Vida Araban ◽  
Zahra Bakhtiary ◽  
...  
Keyword(s):  
Biological Sciences ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Multidisciplinary Approach ◽  
Bone Tissue Regeneration ◽  
Future Perspectives ◽  
Made In

Joint replacement and bone reconstructive surgeries are on the rise globally. Current strategies for implants and bone regeneration are associated with poor integration and healing resulting in repeated surgeries. A multidisciplinary approach involving basic biological sciences, tissue engineering, regenerative medicine and clinical research is required to overcome this problem. Considering the nanostructured nature of bone, expertise and resources available through recent advancements in nanobiotechnology enable researchers to design and fabricate devices and drug delivery systems at the nanoscale to be more compatible with the bone tissue environment. The focus of this review is to present the recent progress made in the rationale and design of nanomaterials for tissue engineering and drug delivery relevant to bone regeneration.

Nanomaterials-based Cell Osteogenic Differentiation and Bone Regeneration

2021 ◽  
Vol 16 (1) ◽  
pp. 36-47
Author(s):  
Tianxu Zhang ◽  
Yang Gao ◽  
Weitong Cui ◽  
Yanjing Li ◽  
Dexuan Xiao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Bone Repair ◽  
Rapid Development ◽  
Physical And Mechanical Properties ◽  
Unique Chemical

With the rapid development of nanotechnology, various nanomaterials have been applied to bone repair and regeneration. Due to the unique chemical, physical and mechanical properties, nanomaterials could promote stem cells osteogenic differentiation, which has great potentials in bone tissue engineering and exploiting nanomaterials-based bone regeneration strategies. In this review, we summarized current nanomaterials with osteo-induction ability, which could be potentially applied to bone tissue engineering. Meanwhile, the unique properties of these nanomaterials and their effects on stem cell osteogenic differentiation are also discussed. Furthermore, possible signaling pathways involved in the nanomaterials- induced cell osteogenic differentiation are also highlighted in this review.

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