scholarly journals Complete Nucleotide Sequence of the First KPC-2- and SHV-12-Encoding IncX Plasmid, pKpS90, from Klebsiella pneumoniae

2012 ◽  
Vol 57 (1) ◽  
pp. 618-620 ◽  
Author(s):  
Najiby Kassis-Chikhani ◽  
Lionel Frangeul ◽  
Laurence Drieux ◽  
Christian Sengelin ◽  
Vincent Jarlier ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Complete Nucleotide Sequence ◽  
Sequence Type ◽  
University Hospital ◽  
Incompatibility Group ◽  
Content Type

ABSTRACTWe report the complete nucleotide sequence of the pKpS90 plasmid, carrying theblaKPC-2andblaSHV-12genes. This plasmid was isolated from a sequence type 258 (ST258)Klebsiella pneumoniaestrain responsible for an outbreak in a French university hospital in 2009. pKpS90 is a 53,286-bp plasmid that belongs to the IncX incompatibility group. pKpS90 consists of a backbone from IncX plasmids, in which the KPC-2-encoding Tn4401transposon and ablaSHV-12-encoding region have been inserted.

scholarly journals Complete Nucleotide Sequence of KPC-3-Encoding Plasmid pKpQIL in the Epidemic Klebsiella pneumoniae Sequence Type 258

2010 ◽  
Vol 54 (10) ◽  
pp. 4493-4496 ◽  
Author(s):  
Azita Leavitt ◽  
Inna Chmelnitsky ◽  
Yehuda Carmeli ◽  
Shiri Navon-Venezia
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Dna Sequence ◽  
Complete Nucleotide Sequence ◽  
Sequence Type ◽  
Incompatibility Group ◽  
Carbapenem Resistant ◽  
Transmissible Plasmid ◽  
Clone Sequence

ABSTRACT We have determined the entire DNA sequence of plasmid pKpQIL, the bla KPC-3-carrying plasmid harbored by the carbapenem-resistant Klebsiella pneumoniae clone sequence type 258 (ST 258) in Israel. pKpQIL is a 113,637-bp, self-transmissible plasmid that belongs to the incompatibility group IncFII. It consists of a large backbone of a pKPN4-like plasmid and carries the bla KPC-3-containing Tn4401a transposon of a pNYC-like plasmid.

scholarly journals Complete Sequence of ablaKPC-2-Harboring IncFIIK1Plasmid from a Klebsiella pneumoniae Sequence Type 258 Strain

2013 ◽  
Vol 57 (3) ◽  
pp. 1542-1545 ◽  
Author(s):  
Liang Chen ◽  
Kalyan D. Chavda ◽  
Roberto G. Melano ◽  
Michael R. Jacobs ◽  
Michael H. Levi ◽  
...  
Keyword(s):  
New York ◽  
New York City ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
York City ◽  
Complete Sequence ◽  
Sequence Type ◽  
Content Type ◽  
City Area ◽  
Carbapenem Resistant

ABSTRACTWe report the nucleotide sequence of a novelblaKPC-2-harboring IncFIIK1plasmid, pBK32179, isolated from a carbapenem-resistantKlebsiella pneumoniaeST258 strain from a New York City patient. pBK32179 is 165 kb long, consists of a large backbone of pKPN3-like plasmid, and carries an 18.5-kbblaKPC-2-containing element that is highly similar to plasmid pKpQIL. pBK32179-like plasmids were identified in 8.3% of strains in a collection of 96K. pneumoniaeisolates from hospitals in the New York City area.

scholarly journals Carbapenemase-Producing Klebsiella pneumoniae, a Key Pathogen Set for Global Nosocomial Dominance

2015 ◽  
Vol 59 (10) ◽  
pp. 5873-5884 ◽  
Author(s):  
Johann D. D. Pitout ◽  
Patrice Nordmann ◽  
Laurent Poirel
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Membrane Permeability ◽  
Recombination Event ◽  
Sequence Type ◽  
Human Pathogen ◽  
Incompatibility Group ◽  
Content Type ◽  
The World ◽  
Outer Membrane Permeability

ABSTRACTThe management of infections due toKlebsiella pneumoniaehas been complicated by the emergence of antimicrobial resistance, especially to carbapenems. Resistance to carbapenems inK. pneumoniaeinvolves multiple mechanisms, including the production of carbapenemases (e.g., KPC, NDM, VIM, OXA-48-like), as well as alterations in outer membrane permeability mediated by the loss of porins and the upregulation of efflux systems. The latter two mechanisms are often combined with high levels of other types of β-lactamases (e.g., AmpC).K. pneumoniaesequence type 258 (ST258) emerged during the early to mid-2000s as an important human pathogen and has spread extensively throughout the world. ST258 comprises two distinct lineages, namely, clades I and II, and it seems that ST258 is a hybrid clone that was created by a large recombination event between ST11 and ST442. Incompatibility group F plasmids withblaKPChave contributed significantly to the success of ST258. The optimal treatment of infections due to carbapenemase-producingK. pneumoniaeremains unknown. Some newer agents show promise for treating infections due to KPC producers; however, effective options for the treatment of NDM producers remain elusive.

scholarly journals Characterization of pKP1433, a Novel KPC-2-Encoding Plasmid from Klebsiella pneumoniae Sequence Type 340

2013 ◽  
Vol 57 (7) ◽  
pp. 3427-3429 ◽  
Author(s):  
C. C. Papagiannitsis ◽  
V. Miriagou ◽  
P. Giakkoupi ◽  
L. S. Tzouvelekis ◽  
A. C. Vatopoulos
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Content Type ◽  
Extensive Sequence

ABSTRACTThe nucleotide sequence of pKP1433 (55,417 bp), ablaKPC-2-carrying plasmid fromKlebsiella pneumoniaesequence type 340, was determined. pKP1433 displayed extensive sequence and structural similarities with the IncN plasmids possessing the KPC-2-encoding Tn4401bisoform. However, the replication, partitioning, and stability of pKP1433 were determined by sequences related to diverse non-IncN plasmids.

scholarly journals Complete Nucleotide Sequence of the IncN Plasmid Encoding IMP-6 and CTX-M-2 from Emerging Carbapenem-Resistant Enterobacteriaceae in Japan

2014 ◽  
Vol 59 (2) ◽  
pp. 1356-1359 ◽  
Author(s):  
Shizuo Kayama ◽  
Norifumi Shigemoto ◽  
Ryuichi Kuwahara ◽  
Kenshiro Oshima ◽  
Hideki Hirakawa ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Dna Sequence ◽  
Complete Nucleotide Sequence ◽  
Carbapenem Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
The Family ◽  
Carbapenem Resistant Enterobacteriaceae

ABSTRACTWe have determined the DNA sequence ofKlebsiella pneumoniaemultidrug resistance plasmid pKPI-6, which is a self-transmissible IncN-type plasmid. pKPI-6 harboringblaIMP-6andblaCTX-M-2confers a stealth-type carbapenem resistance phenotype on members of the familyEnterobacteriaceaethat is not detectable with imipenem. pKPI-6 is already epidemic in Japan, favoring the dissemination of IMP-6 and CTX-M-2 in members of the familyEnterobacteriaceae.

scholarly journals Complete Nucleotide Sequence of pCTX-M360, an Intermediate Plasmid between pEL60 and pCTX-M3, from a Multidrug-Resistant Klebsiella pneumoniae Strain Isolated in China

2009 ◽  
Vol 53 (12) ◽  
pp. 5291-5293 ◽  
Author(s):  
Wen-han Zhu ◽  
Lan Luo ◽  
Jia-yi Wang ◽  
Xiao-hong Zhuang ◽  
Ling Zhong ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Complete Nucleotide Sequence ◽  
Multidrug Resistant ◽  
Incompatibility Group ◽  
Extended Spectrum

ABSTRACT In this work we report the characterization of plasmid pCTX-M360, isolated from a Klebsiella pneumoniae strain from China and encoding the CTX-M-3 extended-spectrum β-lactamase. Sequence analysis of pCTX-M360 revealed extensive similarity with pEL60 and pCTX-M3, two other enterobacterial plasmids of the IncL/M incompatibility group. Compared to pEL60, pCTX-M360 contains several insertions but lacks most of a 27-kb insert found in pCTX-M3, suggesting that it could be an evolutionary intermediate between pEL60 and pCTX-M3.

scholarly journals Complete Nucleotide Sequence of an Escherichia coli Sequence Type 410 Strain Carrying bla NDM-5 on an IncF Multidrug Resistance Plasmid and bla OXA-181 on an IncX3 Plasmid

2018 ◽  
Vol 6 (5) ◽  
Author(s):  
Søren Overballe-Petersen ◽  
Louise Roer ◽  
Kim Ng ◽  
Frank Hansen ◽  
Ulrik S. Justesen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Nucleotide Sequence ◽  
Resistance Genes ◽  
Complete Nucleotide Sequence ◽  
Sequence Type ◽  
Nanopore Sequencing ◽  
Incompatibility Group ◽  
E Coli ◽  
Resistance Plasmid

ABSTRACT Using Nanopore sequencing, we describe here the circular genome of an Escherichia coli sequence type 410 (ST410) strain with five closed plasmids. A large 111-kb incompatibility group F (IncF) plasmid harbored bla NDM-5 and 16 other resistance genes. A 51-kb IncX3 plasmid carried QnrS1 and bla OXA-181 . E. coli isolates with both bla NDM-5 and bla OXA-181 carbapenemases are rare.

scholarly journals Complete Sequence of ablaKPC-Harboring Cointegrate Plasmid Isolated from Escherichia coli

2015 ◽  
Vol 59 (5) ◽  
pp. 2956-2959 ◽  
Author(s):  
Kalyan D. Chavda ◽  
Liang Chen ◽  
Michael R. Jacobs ◽  
Albert D. Rojtman ◽  
Robert A. Bonomo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Horizontal Transfer ◽  
Complete Nucleotide Sequence ◽  
Complete Sequence ◽  
Plasmid Transfer ◽  
Content Type

ABSTRACTHorizontal transfer ofblaKPC-harboring plasmids contributes significantly to the inter- and intraspecies spread ofKlebsiella pneumoniaecarbapenemase (KPC). Here we report the complete nucleotide sequence of ablaKPC-harboring IncFIA plasmid, pBK32533, fromEscherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates howblaKPCcan be spread from a nonconjugative plasmid through cointegration.

scholarly journals Complete Sequence of a Novel IncR-F33:A–:B– Plasmid, pKP1034, HarboringfosA3,blaKPC-2,blaCTX-M-65,blaSHV-12, andrmtBfrom an Epidemic Klebsiella pneumoniae Sequence Type 11 Strain in China

2015 ◽  
Vol 60 (3) ◽  
pp. 1343-1348 ◽  
Author(s):  
Dai-Rong Xiang ◽  
Jun-Jie Li ◽  
Zi-Ke Sheng ◽  
Hai-Ying Yu ◽  
Mei Deng ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Mainland China ◽  
Complete Sequence ◽  
Resistance Rate ◽  
Sequence Type ◽  
University Hospital ◽  
Content Type ◽  
Resistance Determinants ◽  
Fosfomycin Resistance ◽  
Positive Isolate

A high fosfomycin resistance rate was observed inKlebsiella pneumoniaecarbapenemase (KPC)-producingK. pneumoniae(KPC-KP) in our previous study, but little is known about its mechanisms. In this study, we explored the prevalence of plasmid-mediated fosfomycin resistance determinants among fosfomycin-resistant KPC-KP strains from a Chinese university hospital and determined the complete sequence of a novelfosA3-carrying plasmid isolated from an epidemicK. pneumoniaesequence type (ST) 11 strain. A total of 97 KPC-KP strains were studied, of which 57 (58.8%) were resistant to fosfomycin, including 44 (45.4%) harboringfosA3and 1 harboringfosA. AllfosA3-positive strains belonged to the dominant ST11-pulse type (PT) A clone according to multilocus sequence typing and pulsed-field gel electrophoresis, suggesting clonal dissemination. ThefosA-positive isolate belonged to ST11-PTE. ThefosA3-carrying plasmid pKP1034 is 136,848 bp in length and is not self-transmissible. It is a multireplicon plasmid belonging to IncR-F33:A−: B−. BesidesfosA3, a variety of other resistance determinants, includingblaKPC-2,rmtB,blaCTX-M-65, andblaSHV-12, are identified in pKP1034, which would allow for coselection offosA3by most β-lactams and/or aminoglycosides and facilitate its dissemination despite limited use of fosfomycin in China. Detailed comparisons with related plasmids revealed that pKP1034 is highly mosaic and might have evolved from alarming recombination of theblaKPC-2-carrying plasmid pKPC-LK30 from Taiwan and the epidemicfosA3-carrying plasmid pHN7A8 from mainland China.

scholarly journals Complete Nucleotide Sequence of pKOI-34, an IncL/M Plasmid CarryingblaIMP-34in Klebsiella oxytoca Isolated in Japan

2016 ◽  
Vol 60 (5) ◽  
pp. 3156-3162 ◽  
Author(s):  
Norimitsu Shimada ◽  
Shizuo Kayama ◽  
Norifumi Shigemoto ◽  
Junzo Hisatsune ◽  
Ryuichi Kuwahara ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Complete Nucleotide Sequence ◽  
Erwinia Amylovora ◽  
Klebsiella Oxytoca ◽  
Class 1 Integron ◽  
Content Type ◽  
The Core ◽  
Core Module ◽  
Class 1

ABSTRACTWe determined the complete nucleotide sequence of a self-transmissible IncL/M plasmid, pKOI-34, from aKlebsiella oxytocaisolate. pKOI-34 possessed the core structure of an IncL/M plasmid found inErwinia amylovora, pEL60, with two mobile elements inserted, a transposon carrying the arsenic resistance operon and a Tn21-like core module (tnpandmermodules) piggybackingblaIMP-34as a class 1 integron, In808, whereblaIMP-34confers a resistance to carbapenems inK. oxytocaandKlebsiella pneumoniae.

Sign in / Sign up

Export Citation Format

Share Document