scholarly journals Aged HCT-8 Cell Monolayers Support Cryptosporidium parvum Infection

2007 ◽  
Vol 73 (23) ◽  
pp. 7548-7551 ◽  
Author(s):  
Laura Y. Sifuentes ◽  
George D. Di Giovanni
Keyword(s):  
Water Quality ◽  
Cell Culture ◽  
Water Sample ◽  
Cryptosporidium Parvum ◽  
Water Samples ◽  
Sample Collection ◽  
Sample Processing ◽  
Cell Monolayers ◽  
Immunofluorescent Assay ◽  
Naturally Occurring

ABSTRACT Cell culture assays in various formats have been used to study the infectivity of Cryptosporidium spp. as well as to determine the infectivity of naturally occurring oocysts in water. Currently, cell culture assays for infectious Cryptosporidium spp. in water have largely been limited to practice in research laboratories. One obstacle to the routine use of Cryptosporidium cell culture assays for the analysis of water samples is the coordination of water sample collection and processing with readiness of cell culture monolayers. For most Cryptosporidium cell culture assays, monolayers are allowed to develop for 24 to 48 h to reach 80 to 100% confluence prior to inoculation. In this study, we used immunofluorescent assay microscopy to evaluate freshly confluent (2-day-old) and aged (8- to 67-day-old) HCT-8 cell monolayers for their ability to support Cryptosporidium parvum infection. HCT-8 monolayers as old as 67 days were clearly shown to support infection. In two of three experiments, aged monolayers (8- to 11-day-old and 11- to 22-day-old, respectively) developed the same number of C. parvum clusters of infection as freshly confluent monolayers. Results suggest that it may be possible to use cell monolayers from freshly confluent to 3 weeks old on hand for infectivity assays without having to schedule sample processing to coincide with development of freshly confluent monolayers. This would make Cryptosporidium cell culture assays much more feasible for water quality and utility laboratories.

Comparison of Cell Culture and a Poliovirus Gene Probe Assay for the Detection of Enteroviruses in Environmental Water Samples

1993 ◽  
Vol 27 (3-4) ◽  
pp. 311-314 ◽  
Author(s):  
Aaron B. Margolin ◽  
Charles P. Gerba ◽  
Kenneth J. Richardson ◽  
Jaime E. Naranjo
Keyword(s):  
Cell Culture ◽  
Activated Sludge ◽  
Water Samples ◽  
Cdna Probe ◽  
Culture Method ◽  
Tidal River ◽  
Nucleic Acid Hybridization ◽  
Treated Wastewater ◽  
Gene Probe ◽  
Naturally Occurring

Nucleic acid hybridization provides a rapid non-cell culture method for the detection of enteric viruses in water. The purpose of this work was to compare the detection of naturally occurring enteroviruses by cell culture with their detection by a poliovirus gene probe in various types of water samples. Samples of activated sludge effluent, tertiary treated wastewater (activated sludge, filtration and passage through reverse osmosis), ground water, surface water and tidal river water were processed through 1 MDS Virozorb filters to concentrate any naturally occurring virus. Viruses were eluted from the filters with pH 9.5 beef extract and reduced in volume by flocculation to 20-30 ml. These concentrates were then assayed in the BGM cell line by the cytopathogenic effects (CPE) method and by a poliovirus cDNA probe (base pairs 115-7440) labeled with 32P. A total of 233 samples were assayed in this manner. In slightly more than 93% of the samples gene probe and cell culture yielded the same results. Of these samples 36 were positive by gene probe and 28 by cell culture assay. Positive samples for gene probe were confirmed by treatment with NaOH or RNAse and then reprobed. Samples demonstrating CPE upon primary passage were confirmed positive by subsequent passage of cell lysate on a new monolayer of BGM cells. Ten samples were positive by gene probe and negative by cell culture, and 4 samples were negative by gene probe and positive by cell culture.

Evaluation of flow-cytometry for the monitoring of infectious human rotavirus in water

1997 ◽  
Vol 35 (11-12) ◽  
pp. 451-453
Author(s):  
F. X. Abad ◽  
A. Bosch ◽  
J. Comas ◽  
D. Villalba ◽  
R. M. Pintó
Keyword(s):  
Flow Cytometry ◽  
Water Samples ◽  
Wild Type ◽  
Cell Monolayers ◽  
Naturally Occurring ◽  
Human Rotavirus ◽  
Faecal Samples

A method has been developed for the detection of infectious human rotavirus (HRV), based on infection of MA104 and CaCo-2 cell monolayers and ulterior flow cytometry. The sensitivity of the flow cytometry procedure for the cell-adapted HRV enabled the detection of 200 and 2 MPNCU in MA104 and CaCo-2 cells, respectively. Flow cytometry performed five days after infection of CaCo-2 enabled the detection of naturally occurring wild-type HRV in faecal samples and concentrated water samples.

scholarly journals Behavior and Performance of Natural Coagulants in Polluted Water

2020 ◽  
Vol 8 (5) ◽  
pp. 1096-1100
Keyword(s):  
Water Quality ◽  
Water Sample ◽  
Water Samples ◽  
Polluted Water ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Natural Coagulants ◽  
And Performance ◽  
Physical And Chemical ◽  
Domestic Purpose

In this project, Polluted water sample from a Pond is collected for treating it by using Natural Coagulants. The pond is located in Madarpakkam Village, near Gummudipoondi, Tamilnadu, India. There were 4 water samples collected from four different points around the pond at depth of 0.5m, 1m, 1.5m and 2m. The water samples were analyzed for physical and chemical parameters with reference to the code IS 10500-1991. The objective of this project is to reduce the level of turbidity and bacteriological contaminants from water using locally available Natural Coagulants and to reduce the concentration of particular matter such as suspended particles, parasites, bacteria, algae, virus and fungi and to make use of the treated water for domestic purpose, as it reduces cost about 30% to 70%, the usage of these Natural coagulants will be more in future. It is nontoxic and non-corrosive. So, it is safe for human health. The usage of Natural coagulants can be increased in future as it increases the Water quality.

scholarly journals IMPACTS OF DUMPSITE ON SOIL AND GROUNDWATER QUALITY: A CASE STUDY OF ERINFUN COMMUNITY, ADO EKITI, SOUTHWESTERN NIGERIA

2021 ◽  
Vol 5 (2) ◽  
pp. 112-116
Author(s):  
F., F. Akinola ◽  
M., O. Lasisi ◽  
B., S. Awe
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Sample ◽  
Biochemical Oxygen Demand ◽  
Water Samples ◽  
Physicochemical Parameters ◽  
Oxygen Demand ◽  
Soil Samples ◽  
World Health ◽  
Health Organization

Groundwater pollution has increased as a result of poor waste disposal practices in developing countries. The purpose of this study was to determine the levels of physicochemical parameters and heavy metal concentrations in order to investigate the impact of dumpsites on groundwater and soil quality in Erinfun community. Four (4) water samples were collected hand dug well and four (4) soil samples designated Ss1 to Ss4 were collected at distances of 10, 20, 30 and 40 m, respectively, away from the waste dumpsite. Physicochemical parameters and traces such as odour, colour, taste and temperature, as well as Biochemical Oxygen Demand, Chemical Oxygen Demand, Dissolve Oxygen, Total Dissolve Solid, pH, and chloride were measured in collected water samples. Collected soil samples were also analyzed for heavy metals such as Magnesium, Zinc, Iron, Chromium, and Lead. All the physical parameters of the water samples analysed were found not to be within the acceptable limit of World Health Organization and Nigerian Standard of Drinking Water Quality standards. The chemical constituents tested were within the acceptable limit of World Health Organization and Nigerian Standard of Drinking Water Quality except for the Biochemical Oxygen Demand, Chemical Oxygen Demand and Dissolve Oxygen of water samples 1 and 2, respectively. The concentration of trace metals in water sample test were within health limit except for Magnesium and Iron which has the highest concentrations in water sample 1 at 10 m away from dumpsite (61.00 mg/l and 0.46 mg/l). Consequently, open dumpsites are discouraged, and constructed standard landfills with appropriate monitoring guidelines are recommended.

scholarly journals Analisa Kadar Kromium VI [Cr (VI)] Air di Kecamatan Tanggulangin, Sidoarjo

2017 ◽  
Vol 1 (2) ◽  
pp. 55
Author(s):  
Ary Andini
Keyword(s):  
Water Quality ◽  
Water Sample ◽  
Water Samples ◽  
System Error ◽  
Oil Drilling ◽  
Standard Curve ◽  
Chromium Vi ◽  
Drilling System

Tanggulangin is one of the sub-districts in Sidoarjo district affected by Lapindo mudflow caused by oil drilling system error located in the area of Siring, Porong. Therefore it had impacted the water quality in around Lapindo mudflow area. The aim of this research was to analysis the levels of water Cr (VI) Tanggulangin district. Water sample was analyzed by spectrophotometer with wavelength of 540 nm and done using two (2) steps ,which were(1) establishment of standard curve and (2) determination of Cr (VI) in water. Ten (10) water samples were taken from various villages in the subdistrict Tanggulangin. Based on the results on level of water chromium VI [Cr (VI)] analyze showed 4 of 10 samples had levels of water Cr (VI)higher than standard 0,05 mg/L (PP No.82 tahun 2001), those sample were 1, 3,4 and 10.

scholarly journals Efficacy of Glutaraldehyde Disinfectant Against Cryptosporidium parvum in the Presence of Various Organic Soils

2003 ◽  
Vol 86 (1) ◽  
pp. 96-100 ◽  
Author(s):  
James Wilson ◽  
Aaron B Margolin
Keyword(s):  
Cell Culture ◽  
Cryptosporidium Parvum ◽  
Medical Equipment ◽  
Fetal Bovine Serum ◽  
Organic Soil ◽  
Organic Soils ◽  
Cell Monolayers ◽  
Glutaraldehyde Solution ◽  
Fetal Bovine

Abstract The opportunistic protozoan Cryptosporidium parvum is highly resistant to disinfectants, including those specifically used for processing reused medical equipment in hospitals. C. parvum oocysts were dried onto glass and steel grooved penicylinders and challenged with 2.5% glutaraldehyde solution in the presence of 3 types of soil with exposures at 10 min, 90 min, and 10 h. The influence of organic soils on disinfection was measured with 5% fetal bovine serum (FBS), 10% FBS, and 5 mg mucin/mL. An in vitro excystation procedure and cell culture infection assay were used to determine survivability of oocysts after the germicide challenge. In the presence of organic soil, all oocysts removed from carriers excysted and infected cell monolayers after all germicide contact times. However, excystation was observed only from oocysts that received no protection from organic soil after 10 h exposure. In these samples, no infection was observed in the cell monolayers. The results of this research demonstrate the importance of thorough cleaning of medical equipment before disinfection.

scholarly journals Bacteriological and Physicochemical Assessment of Water Quality of the Public Swimming Pools in Klang Valley Malaysia

2020 ◽  
Author(s):  
Muhammad Naim Rosli
Keyword(s):  
Water Quality ◽  
Water Sample ◽  
Water Samples ◽  
Physicochemical Parameters ◽  
National Standard ◽  
World Health ◽  
Personal Hygiene ◽  
Plate Count ◽  
Swimming Pools

Abstract Background: Water is essential for life and it is used for various activities such as drinking, bathing, and recreational purposes and also one of the modes of the transmission of the disease. Objective: The objective of this study to assess the present of bacteriological and the standard of physicochemical quality of swimming pools’ water in Lembah Klang, Malaysia. Methodology: Seven water sample collected from outdoor and indoor swimming pools (hotel, apartment and public swimming pool) in Lembah Klang, Malaysia. The water samples were collected in sterile bottles (30 ml). Physicochemical parameters were determined using water quality testing kit pH & chlorine apparatuses. The water sample than had cultured and incubated. The presence of bacteria was counted by total bacterial count method. Result: The ranges of mean values of the various chlorine level parameters of the selected water samples investigated for the apartment, hotel and municipal were, 0.56±1.01, 0.77±0.95 and 1.19±0.91. All the physicochemical parameters except conductivity values were within the permissible limits of the World Health Organization (WHO) standards 2006 and American National Standard for Water Quality in Public Pools and Spas (ANSI/APSP) 2015. The mean standard plate count of water samples from the selected apartment, hotel, and municipal’s swimming pools were, 28407.14±28469.05, 8192.86±10556.36 and 3257.14±6250.17which above the WHO Guideline limit, thus signifying contamination. Conclusion: The study recommends improvement in personal hygiene of swimmers, adequate cleansing of the pools and enforcement of standards by the government.

scholarly journals Study of interrelationship among water quality parameters in earthen pond and concrete tank

2015 ◽  
Author(s):  
Olawale Ahmed Onada ◽  
Ayoola Olusegun Akinwole ◽  
Emmanuel Kolawale Ajani
Keyword(s):  
Water Quality ◽  
Dissolved Oxygen ◽  
Water Samples ◽  
Water Quality Management ◽  
Quality Parameters ◽  
Water Quality Parameters ◽  
Limiting Factors ◽  
Sample Collection ◽  
Commercial Fish ◽  
Earthen Ponds

One of the major limiting factors in aquaculture production is poor water quality which can negatively affect the yield from aquaculture venture. There is therefore the need to study the rate of interrelationship among key water quality parameters in relation to water quality management and productivity. The level of interaction among dissolved oxygen, ammonia, pH, and temperature in two culturing facilities (Earthen and Concrete ponds) was therefore investigated. Water samples were collected from concrete and earthen fish ponds in a commercial fish farm in Ibadan. The concrete tanks and earthen ponds used for the experiment have the same stocking rate; number of stocked fish; water source and feeding rate and frequency. Water samples were collected randomly from 4 different points in each of the rearing facilities with a sampling bottle in the morning (8.00-8.30am) and evening (5.00-5.30pm) immediately after feeding; this is done weekly for 6weeks. Also the mortality of fish in the studied facilities was recorded. Four different physico-chemical parameters (Temperature, pH, Dissolved oxygen and Ammonia) were analyzed and measured using HATCH analysis water testing kit model FF-1A immediately after water sample collection. Data collected were analysed using ANOVA and correlation and were test for significance at p=0.05. The result showed that, the mean values of pH was (8.15±0.17; 8.69±0.17); temperature (27.95±1.88; 30.21±1.880C) and dissolved oxygen (4.79±3.98; 11.38±3.98mg/l) were obtained in the morning and evening respectively. Mean dissolved oxygen was (7.04±3.98; 9.12±3.98mg/l); pH (7.9±0.17; 8.9±0.17mg/l); and ammonia (0.5±0.24; 2.09±0.24) in concrete tanks and earthen ponds respectively. The highest values of temperature (33.000C); dissolved oxygen (13.00mg/l) were obtained in the evening. The values obtained for dissolved oxygen and temperatures showed significant variations between the time of the day. Mean mortality recorded was (1.2±1.07); (2.6±1.07) in concrete and earthen ponds respectively. Mortality recorded shows positive correlation with temperature and ammonia with correlation coefficient (r=0.18) and (r=0.54) respectively, however, ammonia level had direct significant relationship with mortality. It is concluded that there exist interrelationship among the key water quality parameters examined, and there values varies with time of the day and between different culture facilities.

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