scholarly journals Rediscovering Zygorhizidium affluens Canter: Molecular Taxonomy, Infectious Cycle, and Cryopreservation of a Chytrid Infecting the Bloom-Forming Diatom Asterionella formosa

2018 ◽  
Vol 84 (23) ◽  
Author(s):  
Cecilia Rad-Menéndez ◽  
Mélanie Gerphagnon ◽  
Andrea Garvetto ◽  
Paola Arce ◽  
Yacine Badis ◽  
...  
Keyword(s):  
Molecular Taxonomy ◽  
Cost Effective ◽  
Trophic Levels ◽  
Content Type ◽  
Long Term Storage ◽  
Asterionella Formosa ◽  
Infectious Cycle ◽  
Cryopreservation Method ◽  
Term Storage

ABSTRACT Parasitic Chytridiomycota (chytrids) are ecologically significant in various aquatic ecosystems, notably through their roles in controlling bloom-forming phytoplankton populations and in facilitating the transfer of nutrients from inedible algae to higher trophic levels. The diversity and study of these obligate parasites, while critical to understand the interactions between pathogens and their hosts in the environment, have been hindered by challenges inherent to their isolation and stable long-term maintenance under laboratory conditions. Here, we isolated an obligate chytrid parasite (CCAP 4086/1) on the freshwater bloom-forming diatom Asterionella formosa and characterized its infectious cycle under controlled conditions. Phylogenetic analyses based on 18S, 5.8S, and 28S ribosomal DNAs (rDNAs) revealed that this strain belongs to the recently described clade SW-I within the Lobulomycetales. All morphological features observed agree with the description of the known Asterionella parasite Zygorhizidium affluens Canter. We thus provide a phylogenetic placement for this chytrid and present a robust and simple assay that assesses both the infection success and the viability of the host. We also validate a cryopreservation method for stable and cost-effective long-term storage and demonstrate its recovery after thawing. All the above-mentioned tools establish a new gold standard for the isolation and long-term preservation of parasitic aquatic chytrids, thus opening new perspectives to investigate the diversity of these organisms and their physiology in a controlled laboratory environment. IMPORTANCE Despite their ecological relevance, parasitic aquatic chytrids are understudied, especially due to the challenges associated with their isolation and maintenance in culture. Here we isolated and established a culture of a chytrid parasite infecting the bloom-forming freshwater diatom Asterionella formosa. The chytrid morphology suggests that it corresponds to the Asterionella parasite known as Zygorhizidium affluens. The phylogenetic reconstruction in the present study supports the hypothesis that our Z. affluens isolate belongs to the order Lobulomycetales and clusters within the novel clade SW-I. We also validate a cryopreservation method for stable and cost-effective long-term storage of parasitic chytrids of phytoplankton. The establishment of a monoclonal pathosystem in culture and its successful cryopreservation opens the way to further investigate this ecologically relevant parasitic interaction.

The medium-term prospects for long-term storage systems

2017 ◽  
Vol 35 (1) ◽  
pp. 11-31 ◽  
Author(s):  
David Stuart Holmes Rosenthal
Keyword(s):  
Digital Media ◽  
Media Industry ◽  
Medium Term ◽  
Bulk Storage ◽  
Content Type ◽  
Long Term Storage ◽  
Storage Media ◽  
Technology Changes ◽  
Term Storage

Purpose Increasingly, the content that libraries collect is no longer on paper, a long-lived, medium whose technology changes very slowly and with which they have centuries of experience. Instead, it is stored on relatively short-lived digital media whose technology appears to change rapidly and with which they have little history. The paper aims to discuss this issue. Design/methodology/approach The storage media industry is highly competitive and is currently evolving rapidly as flash, a solid state medium, displaces spinning disk from many applications. Long-term archival storage is a small part of the total storage market. It typically re-uses media and systems intended for more general bulk storage. Findings What are the medium-term prospects for change in this market? Originality/value Much of this material has appeared in blog posts and talks aimed at storage experts, such as the recent DARPA workshop on future of storage. It is presented here for a librarian audience with the necessary additional exposition and background.

scholarly journals Long-Term Storage of Compound Solutions for High-Throughput Screening by Using a Novel 1536-Well Microplate

2009 ◽  
Vol 14 (5) ◽  
pp. 492-498 ◽  
Author(s):  
Martin Joseph Pfeifer ◽  
Guenther Scheel
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Cost Effective ◽  
The Novel ◽  
Long Term Storage ◽  
Plate Design ◽  
Minor Losses ◽  
Storage Times ◽  
Term Storage

This report describes the features and the performance of a new and significantly improved 1536-well microplate design. The design allows for simple, automation-friendly, and cost-effective storage of compound solutions for high-throughput screening. The plate design is based on Society for Biomolecular Sciences standards for microplates and can be molded from polystyrene or cycloolefin copolymer, thus making the plate suitable for use with acoustic dispensing as well as other conventional liquid dispensing in the nanoliter range. For a 9:1 DMSO/water mix as solvent, the novel plate design has shown to perform over 4 months with only minor losses in solvent. Thus, this novel plate design creates the basis for further reductions in compound storage volumes and allows for an increase in the storage times for microliter volumes for up to a year or more. The high protection against solvent evaporation is also visible for aqueous solutions, thus allowing for reduced edge effects during screening campaigns.

scholarly journals The Study of Soybean Chaff Pressing Process Depending on Its Fractional Composition and Humidity

2019 ◽  
Vol 5 (5) ◽  
pp. 160-164 ◽  
Author(s):  
V. Shishkin ◽  
E. Shulzhenko
Keyword(s):  
Fractional Composition ◽  
Complex Mixture ◽  
Cost Effective ◽  
Laboratory Unit ◽  
Long Term Storage ◽  
The Cost ◽  
Far Eastern ◽  
And Storage ◽  
Term Storage

A by-product of soybean production, as a non-grain part of the crop, can be used to meet the needs of livestock in providing complete feeding of livestock. Pressing the floor allows to reduce the volume of plant material in order to reduce the cost of storage and transportation, improve the safety of its nutrients during long-term storage. Therefore, the development of an efficient, cost-effective and less energy-intensive technology and a process line for compacting the soybean floor by pressing is a promising direction. The pile of the floor is a complex mixture of particles of different sizes, as well as voids filled with air. Equipment compaction chaff should provide a stable preparation of pellets under all the fluctuations of the moisture content and fractional composition of chaff. In 2018, research experiments were carried out in the Far Eastern Scientific–Research Institute of Mechanization and Electrification of Agriculture to study the process of pressing the soybean floor, depending on its fractional composition and humidity, on the manufactured laboratory unit for compaction with the production of soybean floor briquettes. It was found that when the humidity of the sample increases from 9 to 21%, the energy consumption decreases by 17.1%. The coefficient of compaction of the floor when the humidity changes almost does not change. By increasing the length of the sex particles from 14 to 87 mm, the compaction coefficient increases by 73.3% and the energy intensity by 6.2 %. For transportation and storage of the soybean floor briquette, it is recommended to pack it tightly with polyethylene film or tie it with twine, this will increase the shelf life and eliminate losses.

scholarly journals Offspring production from cryopreserved primordial germ cells in Drosophila

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Miho Asaoka ◽  
Yurina Sakamaki ◽  
Tatsuya Fukumoto ◽  
Kaori Nishimura ◽  
Masatoshi Tomaru ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Genetic Alteration ◽  
Germline Stem Cells ◽  
Offspring Production ◽  
Long Term Storage ◽  
Long Time ◽  
Cryopreservation Method ◽  
Term Storage

AbstractThere is an urgent need to cryopreserve Drosophila stocks that have been maintained as living cultures for a long time. Long-term culture increases the risk of accidental loss and of unwanted genetic alteration. Here, we report that cryopreserved primordial germ cells (PGCs) can produce F1 progeny when transplanted into hosts. The cryopreserved donor PGCs could form germline stem cells in host gonads and contributed to continuous offspring production. Furthermore, the ability to produce offspring did not appear to vary with either differences between donor strains or cryopreservation duration. Therefore, we propose that our cryopreservation method is feasible for long-term storage of various Drosophila strains. These results underscore the potential usefulness of our cryopreservation method for backing up living stocks to avoid either accidental loss or genetic alteration.

Clinical results of lyophilized human cadaver dura transplantation

1971 ◽  
Vol 34 (6) ◽  
pp. 770-773 ◽  
Author(s):  
William M. Abbott ◽  
Emmett L. Dupree
Keyword(s):  
Clinical Results ◽  
Complication Rates ◽  
Human Cadaver ◽  
Content Type ◽  
Dural Closure ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Neurosurgical Patients ◽  
Term Storage

✓ The clinical results of lyophilized human cadaver dura transplantation in 170 neurosurgical patients show that it is a safe and effective material for dural closure. It is associated with low complication rates and minimal cortical scarring and adhesions. The successful results have been attributed to the minimal foreign body reaction stimulated by freeze-dried tissue. These factors plus its capacity for safe and convenient long-term storage at room temperature make lyophilization the method of choice for preserving dura mater.

scholarly journals Development and Optimization of a Germination Assay and Long-Term Storage for Cannabis sativa Pollen

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 665 ◽  
Author(s):  
Daniel Gaudet ◽  
Narendra Singh Yadav ◽  
Aleksei Sorokin ◽  
Andriy Bilichak ◽  
Igor Kovalchuk
Keyword(s):  
Cannabis Sativa ◽  
Pollen Viability ◽  
Growth Stages ◽  
Long Term Storage ◽  
Whole Wheat ◽  
Germination Assay ◽  
And Storage ◽  
Cryopreservation Method ◽  
Term Storage

Pollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of Cannabis sativa pollen. Additionally, the bicellular nature of cannabis pollen was identified using DAPI (4′,6-diamidino-2-phenylindole) staining. A pollen germination assay was developed to assess cannabis pollen viability and used to demonstrate that pollen collected from different principal growth stages exhibited differential longevity. Finally, a simple and efficient method that employs pollen combined with baked whole wheat flour and subsequent desiccation under vacuum was developed for the long-term cryopreservation of C. sativa pollen. Using this method, pollen viability was maintained in liquid nitrogen after four months, suggesting long-term preservation of cannabis pollen.

scholarly journals Thermoadaptation-Directed Enzyme Evolution in an Error-Prone Thermophile Derived from Geobacillus kaustophilus HTA426

2014 ◽  
Vol 81 (1) ◽  
pp. 149-158 ◽  
Author(s):  
Hirokazu Suzuki ◽  
Jyumpei Kobayashi ◽  
Keisuke Wada ◽  
Megumi Furukawa ◽  
Katsumi Doi
Keyword(s):  
Bacillus Subtilis ◽  
Parent Strain ◽  
Enzyme Evolution ◽  
Denaturation Temperature ◽  
Content Type ◽  
Practical Applications ◽  
Long Term Storage ◽  
Geobacillus Kaustophilus ◽  
Term Storage

ABSTRACTThermostability is an important property of enzymes utilized for practical applications because it allows long-term storage and use as catalysts. In this study, we constructed an error-prone strain of the thermophileGeobacillus kaustophilusHTA426 and investigated thermoadaptation-directed enzyme evolution using the strain. A mutation frequency assay using the antibiotics rifampin and streptomycin revealed thatG. kaustophilushad substantially higher mutability thanEscherichia coliandBacillus subtilis. The predominant mutations inG. kaustophiluswere A · T→G · C and C · G→T · A transitions, implying that the high mutability ofG. kaustophiluswas attributable in part to high-temperature-associated DNA damage during growth. Among the genes that may be involved in DNA repair inG. kaustophilus, deletions of themutSL,mutY,ung, andmfdgenes markedly enhanced mutability. These genes were subsequently deleted to construct an error-prone thermophile that showed much higher (700- to 9,000-fold) mutability than the parent strain. The error-prone strain was auxotrophic for uracil owing to the fact that the strain was deficient in the intrinsicpyrFgene. Although the strain harboringBacillus subtilispyrFwas also essentially auxotrophic, cells became prototrophic after 2 days of culture under uracil starvation, generatingB. subtilisPyrF variants with an enhanced half-denaturation temperature of >10°C. These data suggest that this error-prone strain is a promising host for thermoadaptation-directed evolution to generate thermostable variants from thermolabile enzymes.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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