scholarly journals Effects of Dried Distillers’ Grain on Fecal Prevalence and Growth of Escherichia coli O157 in Batch Culture Fermentations from Cattle

2007 ◽  
Vol 74 (1) ◽  
pp. 38-43 ◽  
Author(s):  
M. E. Jacob ◽  
J. T. Fox ◽  
J. S. Drouillard ◽  
D. G. Renter ◽  
T. G. Nagaraja
Keyword(s):  
Escherichia Coli ◽  
Batch Culture ◽  
Positive Association ◽  
Feedlot Cattle ◽  
Corn Silage ◽  
Distillers Grains ◽  
E Coli ◽  
Dried Distillers Grains ◽  
Distillers Grain ◽  
Dried Distillers Grain

ABSTRACT Distillers’ grains (DG), a by-product of ethanol production, are fed to cattle. Associations between Escherichia coli O157 prevalence and feeding of DG were investigated in feedlot cattle (n = 379) given one of three diets: steam-flaked corn (SFC) and 15% corn silage with 0 or 25% dried distillers’ grains (DDG) or SFC with 5% corn silage and 25% DDG. Ten fecal samples were collected from each pen weekly for 12 weeks to isolate E. coli O157. Cattle fed 25% DDG with 5 or 15% silage had a higher (P = 0.01) prevalence of E. coli O157 than cattle fed a diet without DDG. Batch culture ruminal or fecal microbial fermentations were conducted to evaluate the effect of DDG on E. coli O157 growth. The first study utilized microbial inocula from steers fed SFC or dry-rolled corn with 0 or 25% DDG and included their diet as the substrate. Ruminal microbial fermentations from steers fed DDG had higher E. coli O157 contents than ruminal microbial fermentations from steers fed no DDG (P < 0.05) when no substrate was included. Fecal fermentations showed no DDG effect on E. coli O157 growth. In the second study with DDG as a substrate, ruminal fermentations with 0.5 g DDG had higher (P < 0.01) E. coli O157 concentrations at 24 h than ruminal fermentations with 0, 1, or 2 g DDG. In fecal fermentations, 2 g DDG resulted in a higher concentration (P < 0.05) at 24 h than 0, 0.5, or 1 g DDG. The results indicate that there is a positive association between DDG and E. coli O157 in cattle, and the findings should have important ramifications for food safety.

scholarly journals Inclusion of Dried or Wet Distillers' Grains at Different Levels in Diets of Feedlot Cattle Affects Fecal Shedding of Escherichia coli O157:H7

2010 ◽  
Vol 76 (21) ◽  
pp. 7238-7242 ◽  
Author(s):  
M. E. Jacob ◽  
Z. D. Paddock ◽  
D. G. Renter ◽  
K. F. Lechtenberg ◽  
T. G. Nagaraja
Keyword(s):  
Escherichia Coli ◽  
Control Diet ◽  
Phase 1 ◽  
Feedlot Cattle ◽  
Distillers Grains ◽  
Phase 2 ◽  
Fecal Samples ◽  
Fecal Shedding ◽  
E Coli ◽  
Dried Distillers Grains

ABSTRACT Our objectives were to evaluate the prevalence of Escherichia coli O157:H7 in cattle fed diets supplemented with 20 or 40% dried distillers' grains (DG) (DDG) or wet DG (WDG) and assess whether removing DG from diets before slaughter affected fecal shedding of E. coli O157:H7. Eight hundred forty steers were allocated to 70 pens (12 steers/pen). Treatments were no DG (control), 20% DDG or WDG, and 40% DDG or WDG, and each was replicated in 14 pens. In phase 1, eight floor fecal samples were collected from each pen every 2 weeks for 12 weeks for isolation of E. coli O157:H7 and detection of high shedders. In phase 2, half of the pens with DG were transitioned to the no-DG control diet, and pen floor fecal samples were collected weekly from all pens for 4 weeks. During phase 1, prevalence of E. coli O157:H7 was 20.8% and 3.2% for high shedders. The form of DG had no significant effect on fecal E. coli O157:H7 shedding. The prevalence levels of E. coli O157:H7 and the numbers of high shedders were not different between diets with 0 or 20% DG; however, cattle fed 40% DG had a higher prevalence and more high shedders than cattle fed 0 or 20% DG (P ≤ 0.05). During phase 2, overall and high-shedder prevalence estimates were 3.3% and <0.1%, respectively, and there were no differences between those for different DG forms and inclusion levels or when DG was removed from diets. The form of DG had no impact on E. coli O157:H7; however, fecal shedding was associated with the DG inclusion level.

Effect of backgrounding and transition diets on fecal concentration and strain types of commensalEscherichia coliin beef cattle

2011 ◽  
Vol 91 (3) ◽  
pp. 449-458 ◽  
Author(s):  
R. Su ◽  
K. Munns ◽  
K. A. Beauchemin ◽  
K. Schwartzkopf-Genswein ◽  
L. Jin-Quan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Barley Grain ◽  
Transition Phase ◽  
E Coli ◽  
Strain Characterization ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
Concentrate Inclusion

Su, R., Munns, K., Beauchemin, K. A., Schwartzkopf-Genswein, K., Jin-Quan, L., Topp, E. and Sharma, R. 2011. Effect of backgrounding and transition diets on fecal concentration and strain types of commensal Escherichia coli in beef cattle. Can. J. Anim. Sci. 91: 449–458. The aim of the study was to assess the effect of four different backgrounding diets [55% corn silage in combination with 40% of either temper rolled barley-grain, wheat dried distillers' grain, corn dried distillers' grain or millrun (dry matter basis)] and a transition diet containing increasing amount of concentrate (60 to 90%) on fecal Escherichia coli in feedlot heifers. Heifers were fed backgrounding forage based diets for 91 d and then were shifted to a transition diet for an additional 18 d. Strain characterization of E. coli (n=224) was carried out at time time points over 109 d and indicated that heifers fed the barley–grain diet shed higher (P<0.001) total and tetracycline-resistant (Tetr) E. coli in the backgrounding phase. Of the total E. coli examined (n=224), 70.3% showed resistance to one or more of the 14 antimicrobials examined, which increased to 82.3% by day 109. Among the recovered E. coli, 23 phenotypes and 154 pulsed field gel electrophoresis patterns grouped into 38 clusters indicative of extensive E. coli diversity in heifers. Although supplementation of 40% barley-grain was correlated to higher total E. coli shedding in the backgrounding phase, the backgrounding diets did not influence strain clustering. Strains collected during the transition phase clustered more closely than those collected during the backgrounding phase. This increased strain clustering with high concentrate inclusion in the transition diet was observed within 18 d of the transition phase. Our study indicated variations in E. coli shedding rates and strain clustering in relation to time and diet.

Survival of Escherichia coli O157:H7 in ruminal or fecal contents incubated with corn or wheat dried distillers’ grains with solubles

2010 ◽  
Vol 56 (11) ◽  
pp. 890-895 ◽  
Author(s):  
H.E. Yang ◽  
W.Z. Yang ◽  
J.J. McKinnon ◽  
T.W. Alexander ◽  
Y.L. Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Volatile Fatty Acids ◽  
Barley Grain ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Distillers Grains With Solubles ◽  
The Media ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
Corn Ddgs

Dried distillers’ grain with solubles (DDGS) is a by-product of ethanol production, and its use as cattle feed has increased as a result of the expansion of the fuel ethanol industry. However, the inclusion of corn DDGS into feedlot diets may increase the shedding of Escherichia coli O157:H7. This study investigated whether corn or wheat DDGS at 2 concentrations (20% or 40% vs. 100% barley grain) affected the survival of E. coli O157:H7 in incubations of ruminal digesta and feces. Neither the type nor the level of DDGS had any effect on fermentation or the survival of E. coli O157:H7 in ruminal digesta. However, there was a time by DDGS interaction (p < 0.05), where the numbers of E. coli O157:H7 in feces did not differ after 4 or 12 h of incubation but were greater after 24 h in both 40% wheat and 40% corn DDGS as compared with other treatments. Additionally, after 24 h, the numbers of E. coli O157:H7 were greater in fecal incubations with corn DDGS than with wheat DDGS (p < 0.05). The differences in the numbers of E. coli O157:H7 were not attributable to changes in pH or in concentrations of volatile fatty acids in the media. These results suggest that the inclusion of high levels of corn or wheat DDGS in feedlot diets of cattle may encourage the survival of E. coli O157:H7 in feces.

scholarly journals Factors Associated with the Presence of Coliforms in the Feed and Water of Feedlot Cattle

2005 ◽  
Vol 71 (10) ◽  
pp. 6026-6032 ◽  
Author(s):  
Michael W. Sanderson ◽  
Jan M. Sargeant ◽  
David G. Renter ◽  
D. Dee Griffin ◽  
Robert A. Smith
Keyword(s):  
Positive Association ◽  
Cross Sectional Study ◽  
Feedlot Cattle ◽  
Negative Association ◽  
Corn Silage ◽  
Cross Sectional ◽  
E Coli ◽  
Water Ph ◽  
June July ◽  
Feed Samples

ABSTRACT The objective of this study was to investigate coliform counts in feedlot cattle water and feed rations and their associations with management, climate, fecal material, and water Escherichia coli O157 using a cross-sectional study design. Coliform counts were performed on feed samples from 671 pens on 70 feedlots and on water samples from 702 pens on 72 feedlots in four U.S. states collected between May and August 2001. Management and climate factors were obtained by survey and observation. Month of sampling (higher in May and June), presence of corn silage in the ration (negative association), temperature of the feed 1 in. (ca. 2.5 cm) below the surface at the time of sampling (negative association), and wind velocity at the time of sampling (positive association) were significantly associated with log10 coliform levels in feed. Month of sampling (lower in May versus June July and August), water pH (negative association), and water total solids (positive association) were significantly associated with log10 water coliform levels. Coliform counts in feed and water were not associated with prevalence of E. coli O157 in cattle feces or water. Management risk factors must be interpreted with caution but the results reported here do not support the use of coliform counts as a marker for E. coli O157 contamination of feed or water.

Effect of dried distillers' grain, soybean meal and grain or canola meal and grain-based supplements on forage intake and digestibility

2011 ◽  
Vol 91 (1) ◽  
pp. 123-132 ◽  
Author(s):  
A. Y. Van De Kerckhove ◽  
H. A. Lardner ◽  
P. Yu ◽  
J. J. McKinnon ◽  
K. Walburger
Keyword(s):  
Soybean Meal ◽  
Neutral Detergent Fiber ◽  
Distillers Grains ◽  
Barley Straw ◽  
Canola Meal ◽  
Passage Rate ◽  
Dried Distillers Grains ◽  
Forage Intake ◽  
Distillers Grain ◽  
Dried Distillers Grain

Van De Kerckhove, A. Y., Lardner, H. A., Yu, P., McKinnon J. J. and Walburger, K. 2011. Effect of dried distillers' grain, soybean meal and grain or canola meal and grain-based supplements on forage intake and digestibility. Can. J. Anim. Sci. 91: 123–132. Four ruminally cannulated beef heifers (72 wk of age) were individually fed a basal ration of 75% ground barley straw and 25% ground bromegrass hay [total digestible nutrients=46.3, crude protein (CP)=7.5 (% dry matter (DM))]. Heifers were supplemented with either (1) no supplement (CONT); (2) dried distillers' grains plus solubles [70:30 wheat:corn blend; dried distillers' grains plus solubles (DDGS)]; (3) commercial range pellet (COMM); or (4) barley grain and canola meal (BAR+CM). Forage intake, apparent total tract digestibility, passage rate, rate and extent of forage degradation, rumen pH and rumen ammonia nitrogen were measured. Forage intake, passage rate, and apparent total tract digestibility of DM, neutral detergent fiber, and acid detergent fiber were unaffected (P>0.41) by treatment. Apparent total tract digestibility of CP was increased (P=0.02) with supplements as compared with CONT, but did not differ (P>0.05) among DDGS, COMM, and BAR+CM. Ruminal pH was not affected (P=0.20) by treatment, but rumen ammonia-N was increased (P<0.01) with all three supplements. Potentially degradable and undegradable forage fractions were decreased (P<0.02) and there was a tendency (P=0.06) for the rate of forage DM degradation to increase with supplementation. Supplementing forage diets with either DDGS, grain-soybean-canola- or grain-canola-based supplements did not increase the intake or digestibility of a forage-based diet. More research, however, is required to study the feasibility of feeding these supplements at greater levels with forage-based beef cattle diets.

Fecal Shedding in Cattle Inoculated with Escherichia coli O157:H7 and Fed Corn or Wheat Distillers' Dried Grain with Solubles

2013 ◽  
Vol 76 (1) ◽  
pp. 114-118 ◽  
Author(s):  
J. HALLEWELL ◽  
L. R. BARBIERI ◽  
J. E. THOMAS ◽  
K. STANFORD ◽  
T. A. McALLISTER
Keyword(s):  
Escherichia Coli ◽  
Barley Grain ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Immunomagnetic Bead ◽  
E Coli ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
Feeding Diets ◽  
The Impact

Feeding corn dried distillers' grain with solubles (DDGS) has been linked to increased fecal shedding of Escherichia coli O157:H7 in cattle. A study was conducted to compare the impact of three diets containing (dry matter basis) 40% corn DDGS, 40% wheat DDGS, or 20% corn and 20% wheat mixed DDGS to a standard barley grain finishing diet on fecal shedding in cattle challenged with a 1010 CFU mixture of four nalidixic acid–resistant E. coli O157:H7 strains. Rectal grab samples (n = 544) were collected over 70 days and screened for E. coli O157:H7 by direct plating and immunomagnetic bead separation. Feeding diets containing DDGS had no effect (P &gt; 0.05) on the intensity or duration of fecal shedding of E. coli O157:H7 compared with the standard barley grain finishing diet.

Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets with or without Wet Distillers Grains with Solubles†

2009 ◽  
Vol 72 (8) ◽  
pp. 1624-1633 ◽  
Author(s):  
J. E. WELLS ◽  
S. D. SHACKELFORD ◽  
E. D. BERRY ◽  
N. KALCHAYANAND ◽  
M. N. GUERINI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Distillers Grains ◽  
Escherichia Coli O157 ◽  
Finishing Cattle ◽  
Average Percentage ◽  
E Coli ◽  
Distillers Grains With Solubles ◽  
Phase 0 ◽  
Wet Distillers Grains

The objective of this study was to determine if wet distillers grains with solubles (WDGS) from corn in diets affected Escherichia coli O157:H7 in growing and finishing cattle; steers (n = 603) were randomly assigned to diets with or without WDGS. Hide and fecal samples were collected monthly (October through June) from each animal for enumeration and enrichment of E. coli O157:H7. In the growing phase (0 or 13.9% WDGS diets), fecal prevalence for E. coli O157:H7 in steers fed a diet with WDGS was twice that of the prevalence in control steers (P &lt; 0.001). In the finishing phase (0 or 40% WDGS diets), the average prevalence in feces (P &lt; 0.001) and on hides (P &lt; 0.001) was higher for cattle fed WDGS. The average percentage of fecal E. coli O157:H7 enumerable samples during the finishing phase for cattle fed WDGS was 2.7% compared with 0.1% for control steers (P &lt; 0.001). The average percentage of E. coli O157:H7 enumerable hide samples was not different between diets, but the cattle fed WDGS had higher levels (P &lt; 0.05) of the pathogen. Animals fed WDGS had higher levels of E. coli (P &lt; 0.001), higher pH values (P &lt; 0.001), and lower concentrations of l-lactate (P &lt; 0.001) in feces than those values of the control steers. These results indicate that feeding 40% WDGS could increase the level and prevalence of E. coli O157:H7 in and on feedlot cattle when E. coli O157:H7 is seasonally low.

22 Effects of Housing Cow-calf Pairs on Drylots vs Pasture on Calf Performance and Behavior Through Weaning

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 24-24
Author(s):  
Megan Myerscough ◽  
Wes Chapple ◽  
William T Meteer ◽  
Daniel W Shike
Keyword(s):  
Corn Silage ◽  
Time Interaction ◽  
Creep Feed ◽  
Calf Performance ◽  
Ad Libitum ◽  
Cow Calf ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
And Behavior ◽  
Fence Line

Abstract The objectives were to analyze the effects of housing cow-calf pairs in drylots or pasture on calf performance and behavior through weaning. Simmental × Angus (2 yr; 108/yr; 81 ± 15.3 d postpartum) spring-calving cows were stratified by age, BW, BCS, and calf sex and allotted to six groups/yr. Groups were randomly allotted to 1 of 2 treatments: drylot (DL) or pasture (PAST). Calves in the drylot had ad libitum access to a diet consisting of corn silage, dried distillers grain, corn stalks, dry rolled corn, and soybean hulls. Calves on PAST received creep feed three weeks prior to weaning. Calf BW was measured on d 0, 55, and 110. Calves were fence-line weaned on d 110. Behavior was observed for two days after weaning. Data were analyzed using the MIXED procedure of SAS. Drylot calves had greater (P &lt; 0.01) BW at d 55 and 110. There was treatment by time interactions (P &lt; 0.01) for lying and eating on d 111. More PAST calves were lying at h 2 and 3 and eating at h 11 and 12. More DL calves were lying at h 9, 10, 11, and 12. More DL calves were eating at h 2 and tended to at h 3. There was also a treatment by time interaction (P &lt; 0.01) for vocalizations on d 111. More PAST calves vocalized at h 1, however, DL calves vocalized more at h 4 and tended to at h 5. There was treatment by time interactions (P &lt; 0.01) for eating and walking on d 112. More DL calves were eating at h 2, 3, 4, 7, and 9. More PAST calves were walking at h 1 and 2. There was also a treatment by time interaction (P &lt; 0.01) for vocalizations on d 112. More PAST calves vocalized at h 1 and 10. In conclusion, housing cow-calf pairs in drylots improved preweaning performance and altered postweaning behavior.

Monitoring Escherichia coli O157:H7 in Inoculated and Naturally Colonized Feedlot Cattle and Their Environment

2005 ◽  
Vol 68 (1) ◽  
pp. 26-33 ◽  
Author(s):  
K. STANFORD ◽  
S. J. BACH ◽  
T. H. MARX ◽  
S. JONES ◽  
J. R. HANSEN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Mitigation Strategies ◽  
Escherichia Coli O157 ◽  
Feeding Period ◽  
Sample Collection ◽  
E Coli ◽  
Challenge Study ◽  
On Farm ◽  
Feed Samples

On-farm methods of monitoring Escherichia coli O157:H7 were assessed in 30 experimentally inoculated steers housed in four pens over a 12-week period and in 202,878 naturally colonized feedlot cattle housed in 1,160 pens on four commercial Alberta feedlots over a 1-year period. In the challenge study, yearling steers were experimentally inoculated with 1010 CFU of a four-strain mixture of nalidixic acid–resistant E. coli O157:H7. After inoculation, shedding of E. coli O157:H7 was monitored weekly by collecting rectal fecal samples (FEC), oral swabs (ORL), pooled fecal pats (PAT), manila ropes (ROP) orally accessed for 4 h, feed samples, water, and water bowl interface. Collection of FEC from all animals per pen provided superior isolation (P &lt; 0.01) of E. coli O157:H7 compared with other methods, although labor and animal restraint requirements for fecal sample collection were high. When one sample was collected per pen of animals, E. coli O157:H7 was more likely to be detected from the ROP than from the FEC, PAT, or ORL (P &lt; 0.001). In the commercial feedlot study, samples were limited to ROP and PAT, and E. coli O157:H7 was isolated in 18.8% of PAT and 6.8% of ROP samples. However, for animals that had been resident in the feedlot pen for at least 1 month, isolation of E. coli O157:H7 from ROP was not different from that from PAT (P = 0.35). Pens of animals on feed for &lt;30 days were six times more likely to shed E. coli O157:H7 than were animals on feed for &gt;30 days. However, change in diet did not affect shedding of the organism (P &gt; 0.23) provided that animals had acclimated to the feedlot for 1 month or longer. Findings from this study indicate the importance of introduction of mitigation strategies early in the feeding period to reduce transference and the degree to which E. coli O157:H7 is shed into the environment.

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