scholarly journals Dual Role of the Oligopeptide Permease Opp3 during Growth of Staphylococcus aureus in Milk

2009 ◽  
Vol 75 (10) ◽  
pp. 3355-3357 ◽  
Author(s):  
Elise Borez�e-Durant ◽  
Aurelia Hiron ◽  
Jean-Christophe Piard ◽  
Vincent Juillard
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Nitrogen Nutrition ◽  
Dual Role ◽  
Diauxic Growth ◽  
Extracellular Proteases ◽  
Expression Levels ◽  
Oligopeptide Permease

ABSTRACT Staphylococcus aureus RN6390 presents a diauxic growth in milk, due to amino acid limitation. Inactivation of the oligopeptide permease Opp3 (dedicated to the nitrogen nutrition of the strain) not only affects the growth of the strain but also results in reduced expression levels of three major extracellular proteases.

Dual role of D-amino acid oxidase in experimental pain models

2019 ◽  
Vol 855 ◽  
pp. 98-102 ◽  
Author(s):  
Jasmine Sharma ◽  
Ritu Kulshrestha ◽  
Nirmal Singh ◽  
Amteshwar Singh Jaggi
Keyword(s):  
Amino Acid ◽  
Experimental Pain ◽  
Dual Role ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Pain Models

scholarly journals Expression of the Biofilm-Associated Genes in Methicillin-Resistant Staphylococcus aureus in Biofilm and Planktonic Conditions

2018 ◽  
Vol 19 (11) ◽  
pp. 3487 ◽  
Author(s):  
Barbara Kot ◽  
Hubert Sytykiewicz ◽  
Iwona Sprawka
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Pcr Analysis ◽  
Gene Encoding ◽  
Methicillin Resistant ◽  
Expression Levels ◽  
Fibrinogen Binding ◽  
Genes Encoding ◽  
Laminin Binding Protein

The role of genes that are essential for development of Staphylococcus aureus biofilm during infection is not fully known. mRNA from two methicillin-resistant S. aureus strains that formed weak and strong biofilm on polystyrene plates were isolated at five time points from cells grown in biofilm and planktonic culture. Quantitative real-time PCR analysis showed that the expression levels of investigated genes under biofilm conditions were significantly higher than under planktonic conditions. The expression levels of the gene encoding elastin binding protein (ebps) and laminin binding protein (eno) were significantly increased in biofilm at 3 h, both in strongly and weakly adhering strain. The peak expression of fib gene encoding fibrinogen binding protein was found at 6 and 8 h in the case of strongly and weakly adhering strain, respectively. The expression of icaA and icaD genes in both strains was significantly higher under biofilm conditions when comparing to planktonic cells during 12 h. The expression level of the genes encoding binding proteins and the glucosamine polymer polysaccharide intercellular adhesin (PIA) slowly decreased after 24 h. Finally, we found that the expression levels of genes encoding binding factors in weakly adhering strain were significantly lower than in strongly adhering strain.

scholarly journals Role of the (p)ppGpp Synthase RSH, a RelA/SpoT Homolog, in Stringent Response and Virulence of Staphylococcus aureus

2010 ◽  
Vol 78 (5) ◽  
pp. 1873-1883 ◽  
Author(s):  
Tobias Geiger ◽  
Christiane Goerke ◽  
Michaela Fritz ◽  
Tina Schäfer ◽  
Knut Ohlsen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Acid Metabolism ◽  
Stringent Response ◽  
Essential Amino Acids ◽  
Transcriptional Analysis ◽  
Wild Type ◽  
Stringent Control ◽  
Nutrient Limitations

ABSTRACT In most bacteria, nutrient limitations provoke the stringent control through the rapid synthesis of the alarmones pppGpp and ppGpp. Little is known about the stringent control in the human pathogen Staphylococcus aureus, partly due to the essentiality of the major (p)ppGpp synthase/hydrolase enzyme RSH (RelA/SpoT homolog). Here, we show that mutants defective only in the synthase domain of RSH (rsh syn) are not impaired in growth under nutrient-rich conditions. However, these mutants were more sensitive toward mupirocin and were impaired in survival when essential amino acids were depleted from the medium. RSH is the major enzyme responsible for (p)ppGpp synthesis in response to amino acid deprivation (lack of Leu/Val) or mupirocin treatment. Transcriptional analysis showed that the RSH-dependent stringent control in S. aureus is characterized by repression of genes whose products are predicted to be involved in the translation machinery and by upregulation of genes coding for enzymes involved in amino acid metabolism and transport which are controlled by the repressor CodY. Amino acid starvation also provoked stabilization of the RNAs coding for major virulence regulators, such as SaeRS and SarA, independently of RSH. In an animal model, the rsh syn mutant was shown to be less virulent than the wild type. Virulence could be restored by the introduction of a codY mutation into the rsh syn mutant. These results indicate that stringent conditions are present during infection and that RSH-dependent derepression of CodY-regulated genes is essential for virulence in S. aureus.

scholarly journals The Role of Amino Acid Substitution in HepT Toward Menaquinone Isoprenoid Chain Length Definition and Lysocin E Sensitivity in Staphylococcus aureus

2020 ◽  
Vol 11 ◽  
Author(s):  
Suresh Panthee ◽  
Atmika Paudel ◽  
Hiroshi Hamamoto ◽  
Anne-Catrin Uhlemann ◽  
Kazuhisa Sekimizu
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Chain Length ◽  
Amino Acid Substitution

Role of the hprT–ftsH locus in Staphylococcus aureus

Microbiology ◽  
2004 ◽  
Vol 150 (2) ◽  
pp. 373-381 ◽  
Author(s):  
James K. Lithgow ◽  
Eileen Ingham ◽  
Simon J. Foster
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Methyl Viologen ◽  
Potassium Tellurite ◽  
Mutagenesis Screen ◽  
Acid Methyl ◽  
Starvation Survival ◽  
Membrane Bound ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

The roles of two adjacent genes in the Staphylococcus aureus chromosome with functions in starvation survival and the response to stressful conditions have been characterized. One of these, hprT, encoding a hypoxanthine–guanine phosphoribosyltransferase homologue, was initially identified in a transposon mutagenesis screen. Mutation of hprT affects starvation survival in amino-acid-limiting conditions and the ability of S. aureus to grow in high-salt concentrations. Downstream of hprT is ftsH, which encodes a membrane-bound, ATP- and Zn2+-dependent ‘AAA’-type protease. Mutation of ftsH in S. aureus leads to pleiotropic defects including slower growth, sensitivity to salt, acid, methyl viologen and potassium tellurite stresses, and reduced survival in amino-acid- or phosphate-limiting conditions. Both hprT–lacZ and ftsH–lacZ gene fusions are expressed maximally in the post-exponential phase of growth. Although secretion of exoproteins is not affected, an ftsH mutant is attenuated in a murine skin lesion model of pathogenicity.

The Dual Role of Practitioner and Member of the Academic Community: Comment.

1970 ◽  
Vol 1 (3) ◽  
pp. 281-281
Author(s):  
Jean Paul Smith
Keyword(s):  
Academic Community ◽  
Dual Role

Dual role of B7 costimulation in obesity-related non-alcoholic steatohepatitis (NASH) and metabolic dysregulation

2014 ◽  
Vol 122 (03) ◽  
Author(s):  
A Chatzigeorgiou ◽  
R Garcia-Martin ◽  
KJ Chung ◽  
I Alexaki ◽  
A Klotzsche-von Ameln ◽  
...  
Keyword(s):  
Dual Role ◽  
Alcoholic Steatohepatitis ◽  
Metabolic Dysregulation
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