scholarly journals Microbial Enterotypes, Inferred by the Prevotella-to-Bacteroides Ratio, Remained Stable during a 6-Month Randomized Controlled Diet Intervention with the New Nordic Diet

2013 ◽  
Vol 80 (3) ◽  
pp. 1142-1149 ◽  
Author(s):  
Henrik M. Roager ◽  
Tine R. Licht ◽  
Sanne K. Poulsen ◽  
Thomas M. Larsen ◽  
Martin I. Bahl
Keyword(s):  
Gut Microbiota ◽  
Quantitative Pcr ◽  
Dietary Intervention ◽  
Plasma Cholesterol ◽  
Biological Significance ◽  
Pcr Analysis ◽  
Dietary Interventions ◽  
Content Type ◽  
Nordic Diet ◽  
New Nordic Diet

ABSTRACTIt has been suggested that the human gut microbiota can be divided into enterotypes based on the abundance of specific bacterial groups; however, the biological significance and stability of these enterotypes remain unresolved. Here, we demonstrated that subjects (n= 62) 18 to 65 years old with central obesity and components of metabolic syndrome could be grouped into two discrete groups simply by their relative abundance ofPrevotellaspp. divided byBacteroidesspp. (P/Bratio) obtained by quantitative PCR analysis. Furthermore, we showed that these groups remained stable during a 6-month, controlled dietary intervention, where the effect of consuming a diet in accord with the new Nordic diet (NND) recommendations as opposed to consuming the average Danish diet (ADD) on the gut microbiota was investigated. In this study, subjects (with and without stratification according toP/Bratio) did not reveal significant changes in 35 selected bacterial taxa quantified by quantitative PCR (ADD compared to NND) resulting from the dietary interventions. However, we found higher total plasma cholesterol within the high-P/Bgroup than in the low-P/Bgroup after the intervention. We propose that stratification of humans based simply on theirP/Bratio could allow better assessment of possible effects of interventions on the gut microbiota and physiological biomarkers.

Living with the New Nordic Diet

2014 ◽  
Vol 116 (8) ◽  
pp. 1247-1258 ◽  
Author(s):  
Arun Micheelsen ◽  
Lotte Holm ◽  
Katherine O’Doherty Jensen
Keyword(s):  
Public Health ◽  
Dietary Intervention ◽  
Research Centre ◽  
Dietary Recommendations ◽  
Sociological Study ◽  
Dietary Compliance ◽  
Content Type ◽  
Nordic Diet ◽  
New Nordic Diet ◽  
Dietary Intervention Trial

Purpose – Based on New Nordic Cuisine and Nordic dietary recommendations, the research centre OPUS has developed a healthy, sustainable and tasty New Nordic Diet (NND) with the goal of improving public health in Denmark. In order to determine the health potential of the NND, a six-month, controlled dietary intervention trial was conducted, in which participants procured NND foods at a specially designed intervention supermarket and prepared and consumed NND meals in their homes. The paper aims to discuss these issues. Design/methodology/approach – A qualitative sociological study was conducted among intervention participants in order to explore whether and how they appropriated this diet into their everyday food practices. Findings – Participants appropriated the NND by becoming co-producers of this diet, tailoring it to accord with individual preferences and the demands of everyday life. Findings indicate that while the taste of the NND is likely to appeal to wider groups of consumers, the tasks of food procurement and preparation are likely to constitute barriers to its adoption. The strictly controlled intervention setting enabled participants to learn how to comply with dietary composition despite their deviations from given menu-plans and recipes. The extent to which such compliance would be achieved outside this context by other consumers is questioned. Further studies are needed to determine whether more widespread consumption of the NND would in practice comply with Nordic dietary recommendations. Originality/value – The study provides insights relevant to health agencies, public health researchers and food companies regarding consumer appropriation of a dietary system, the health benefits of which are dependent upon dietary compliance.

scholarly journals Individualized Responses of Gut Microbiota to Dietary Intervention Modeled in Humanized Mice

mSystems ◽  
2016 ◽  
Vol 1 (5) ◽  
Author(s):  
Samuel A. Smits ◽  
Angela Marcobal ◽  
Steven Higginbottom ◽  
Justin L. Sonnenburg ◽  
Purna C. Kashyap
Keyword(s):  
Gut Microbiota ◽  
Dietary Intervention ◽  
Scientific Evidence ◽  
Marker Gene ◽  
Gastrointestinal Symptoms ◽  
Dietary Interventions ◽  
Inflammatory Bowel ◽  
Irritable Bowel ◽  
And Function ◽  
Generation Sequencing

ABSTRACT Dietary modification has long been used empirically to modify symptoms in inflammatory bowel disease, irritable bowel syndrome, and a diverse group of diseases with gastrointestinal symptoms. There is both anecdotal and scientific evidence to suggest that individuals respond quite differently to similar dietary changes, and the highly individualized nature of the gut microbiota makes it a prime candidate for these differences. To overcome the typical confounding factors of human dietary interventions, here we employ ex-germfree mice colonized by microbiotas of three different humans to test how different microbiotas respond to a defined change in carbohydrate content of diet by measuring changes in microbiota composition and function using marker gene-based next-generation sequencing and metabolomics. Our findings suggest that the same diet has very different effects on each microbiota’s membership and function, which may in turn explain interindividual differences in response to a dietary ingredient. Diet plays an important role in shaping the structure and function of the gut microbiota. The microbes and microbial products in turn can influence various aspects of host physiology. One promising route to affect host function and restore health is by altering the gut microbiome using dietary intervention. The individuality of the microbiome may pose a significant challenge, so we sought to determine how different microbiotas respond to the same dietary intervention in a controlled setting. We modeled gut microbiotas from three healthy donors in germfree mice and defined compositional and functional alteration following a change in dietary microbiota-accessible carbohydrates (MACs). The three gut communities exhibited responses that differed markedly in magnitude and in the composition of microbiota-derived metabolites. Adjustments in community membership did not correspond to the magnitude of changes in the microbial metabolites, highlighting potential challenges in predicting functional responses from compositional data and the need to assess multiple microbiota parameters following dietary interventions. IMPORTANCE Dietary modification has long been used empirically to modify symptoms in inflammatory bowel disease, irritable bowel syndrome, and a diverse group of diseases with gastrointestinal symptoms. There is both anecdotal and scientific evidence to suggest that individuals respond quite differently to similar dietary changes, and the highly individualized nature of the gut microbiota makes it a prime candidate for these differences. To overcome the typical confounding factors of human dietary interventions, here we employ ex-germfree mice colonized by microbiotas of three different humans to test how different microbiotas respond to a defined change in carbohydrate content of diet by measuring changes in microbiota composition and function using marker gene-based next-generation sequencing and metabolomics. Our findings suggest that the same diet has very different effects on each microbiota’s membership and function, which may in turn explain interindividual differences in response to a dietary ingredient. Author Video: An author video summary of this article is available.

Cost of New Nordic Diet school meals

2015 ◽  
Vol 117 (9) ◽  
pp. 2372-2386
Author(s):  
Jørgen Dejgård Jensen ◽  
Anne Vibeke Thorsen ◽  
Camilla Trab Damsgaard ◽  
Anja Biltoft-Jensen
Keyword(s):  
Food Waste ◽  
Organic Content ◽  
Portion Size ◽  
Economic Optimization ◽  
School Meals ◽  
Content Type ◽  
School Meal ◽  
Nordic Diet ◽  
Cost Differential ◽  
New Nordic Diet

Purpose – The purpose of this paper is to conduct economic evaluation of a school meal programme based on principles of a New Nordic Diet (NND) by assessing the costs of the NND lunch, compared with packed lunch from home, and investigating potential effects of adjusting the NND principles underlying the school meals on the costs and on the rate of food waste. Design/methodology/approach – The analysis combines recipes, dietary records and food waste data from a school meal intervention with collected price data within an economic optimization framework. Findings – A New Nordic School meal programme consisting of a morning snack and a hot lunch based on fixed seasonal menu plans and with 75 per cent organic content is 37 per cent more expensive in terms of ingredient costs than corresponding packed school meals. This cost differential can be almost halved by introducing more flexible scheduling of week plans and reducing the level of organic ambition to 60 per cent. Reducing portion sizes could reduce the cost differential by an extra 5 per cent, which would also reduce food waste by about 15 per cent. Originality/value – Higher costs and food waste in a restrictive ingredient sourcing school meal programme can be reduced by increased flexibility in meal scheduling, reduction in organic content and reduced average portion size.

scholarly journals Impact of Ceftiofur Injection on Gut Microbiota and Escherichia coli Resistance in Pigs

2015 ◽  
Vol 59 (9) ◽  
pp. 5171-5180 ◽  
Author(s):  
M. A. Fleury ◽  
G. Mourand ◽  
E. Jouy ◽  
F. Touzain ◽  
L. Le Devendec ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gut Microbiota ◽  
Treated Group ◽  
Conjugative Plasmid ◽  
Health Concern ◽  
Pcr Analysis ◽  
Contamination Level ◽  
Content Type ◽  
E Coli ◽  
The Impact

ABSTRACTResistance to extended-spectrum cephalosporins (ESCs) is an important health concern. Here, we studied the impact of the administration of a long-acting form of ceftiofur on the pig gut microbiota and ESC resistance inEscherichia coli. Pigs were orally inoculated with an ESC-resistantE. coliM63 strain harboring a conjugative plasmid carrying a gene conferring resistance,blaCTX-M-1. On the same day, they were given or not a unique injection of ceftiofur. Fecal microbiota were studied using quantitative PCR analysis of the main bacterial groups and quantification of short-chain fatty acids.E. coliand ESC-resistantE. coliwere determined by culture methods, and the ESC-resistantE. coliisolates were characterized. The copies of theblaCTX-M-1gene were quantified. After ceftiofur injection, the main change in gut microbiota was the significant but transitory decrease in theE. colipopulation. Acetate and butyrate levels were significantly lower in the treated group. In all inoculated groups,E. coliM63 persisted in most pigs, and theblaCTX-M-1gene was transferred to otherE. coli. Culture and PCR results showed that the ceftiofur-treated group shed significantly more resistant strains 1 and 3 days after ESC injection. Thereafter, on most dates, there were no differences between the groups, but notably, one pig in the nontreated group regularly excreted very high numbers of ESC-resistantE. coli, probably leading to a higher contamination level in its pen. In conclusion, the use of ESCs, and also the presence of high-shedding animals, are important features in the spread of ESC resistance.

scholarly journals Arr-cb Is a Rifampin Resistance Determinant Found Active or Cryptic in Clostridiumbolteae Strains

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Jean-Christophe Marvaud ◽  
Thierry Lambert
Keyword(s):  
Escherichia Coli ◽  
Gut Microbiota ◽  
Reverse Transcription ◽  
Recent Analysis ◽  
Pcr Analysis ◽  
Directed Mutagenesis ◽  
Human Gut ◽  
Content Type ◽  
Reverse Transcription Pcr ◽  
Rifampin Resistance

ABSTRACT Clostridium bolteae, which belongs to the Clostridium clostridioforme complex, is a member of the human gut microbiota. Recent analysis of seven genomes of C. bolteae revealed the presence of an arr-like gene. Among these strains, only 90A7 was found to be resistant to rifampin in the absence of alteration of RpoB. Cloning of arr-cb from 90A7 in Escherichia coli combined with directed mutagenesis demonstrated that Arr-cb was functional but that a Q127→R variant present in 90A9 and 90B3 was inactive. Quantitative reverse transcription-PCR analysis indicated that arr-cb was silent in the four remaining strains because of defective transcription. Thus, two independent mechanisms can make the probably intrinsic arr-cb gene of C. bolteae cryptic.

Effects of food additives on gut microbiota: friend or foe?

2019 ◽  
Vol 49 (5) ◽  
pp. 955-964
Author(s):  
Elif Inan-Eroglu ◽  
Aylin Ayaz
Keyword(s):  
Gut Microbiota ◽  
Food Additives ◽  
Experimental Studies ◽  
Metabolic Effects ◽  
Artificial Sweeteners ◽  
Processed Foods ◽  
Dietary Interventions ◽  
Content Type ◽  
Model Studies

PurposeRecent evidence suggests that especially processed foods may lead to undesirable metabolic effects in gut microbiota. The emulsifiers and artificial sweeteners that are added to processed foods may play a role in the progression of the diseases through the modulation of microbiota in mice. In this context, the purpose of this paper is to evaluate the effects of emulsifiers and artificial sweeteners.Design/methodology/approachThis paper presents a narrative review of the effects of emulsifiers and artificial sweeteners which are mainly in consumed in the Western diet, to the gut microbiota by mainly focusing on the experimental studies.FindingsAlthoughin vivostudies and animal model studies showed various adverse effects of sweeteners and emulsifiers to microbiota, studies should be conducted in humans to investigate the effects of these food additives to human microbiota by making dietary interventions in the context of ethical rules.Originality/valueIn future, studies will allow us to draw more definitive conclusion whether human population consuming sweeteners and emulsifiers are at risk.

scholarly journals Modulating the gut microbiota of humans by dietary intervention with plant glycans.

2020 ◽  
pp. AEM.02757-20
Author(s):  
Gerald W. Tannock
Keyword(s):  
Gut Microbiota ◽  
Dietary Intervention ◽  
Well Being ◽  
Human Colon ◽  
Molecular Signaling ◽  
Dietary Interventions ◽  
Additional Energy ◽  
Chemical Complexity ◽  
Bacterial Consortia ◽  
Physiological Processes

The human colon contains a community of microbial species, mostly bacteria, which is often referred to as the gut microbiota. The community is considered essential to human well-being by conferring additional energy harvesting capacity, niche exclusion of pathogens, and molecular signaling activities that are integrated into human physiological processes. Plant polysaccharides (glycans, dietary fiber) are an important source of carbon and energy that supports the maintenance and functioning of the gut microbiota. Therefore, the daily quantity and quality of plant glycans consumed by the human host has the potential to influence health. Members of the gut microbiota differ in ability to utilize different types of plant glycans. Dietary interventions with specific glycans could modulate the microbiota, counteracting ecological perturbations that disrupt the intricate relationships between microbiota and host (dysbiosis). This review considers prospects and research options for modulation of the gut microbiota by the formulation of diets that, when consumed habitually, would correct dysbiosis by building diverse consortia that boost functional resilience. Traditional ‘prebiotics’ favor bifidobacteria and lactobacilli, whereas dietary mixtures of plant glycans that are varied in chemical complexity would promote high diversity microbiotas. It is concluded that research should aim at improving knowledge of bacterial consortia that, through shared nourishment, degrade and ferment plant glycans. The consortia may vary in composition from person to person, but functional outputs will be consistent in a given context because of metabolic redundancy among bacteria. Thus the individuality of gut microbiotas could be encompassed, functional resilience encouraged, and correction of dysbiosis achieved.

scholarly journals Comparison of the Effects of Environmental Parameters on Growth Rates of Vibrio vulnificus Biotypes I, II, and III by Culture and Quantitative PCR Analysis

2011 ◽  
Vol 77 (12) ◽  
pp. 4200-4207 ◽  
Author(s):  
Eva Chase ◽  
Valerie J. Harwood
Keyword(s):  
Quantitative Pcr ◽  
Growth Rates ◽  
Environmental Changes ◽  
Vibrio Vulnificus ◽  
Environmental Parameters ◽  
Defined Medium ◽  
Pcr Analysis ◽  
Human Pathogens ◽  
Content Type ◽  
Specific Growth Rates

ABSTRACTVibrio vulnificusis a natural inhabitant of estuarine waters. The three known biotypes include (i) most human pathogens, (ii) primarily eel pathogens, and (iii) pathogens associated with fish and with human wound infections in Israel. Despite the frequently lethal consequences ofV. vulnificusinfections, the growth rates of the various biotypes and their response to environmental changes are not well characterized. We compared the specific growth rates (μ) of a representative of each biotype by culture and quantitative PCR (qPCR) analysis in a defined medium under varied pH, temperature, and salinity. Growth rates based on culturable concentrations were always higher than those based on qPCR estimates; however, both enumeration methods yielded comparable results on the influence of environmental factors on growth rates. Temperature (25°C, 30°C, 37°C), pH (7.0, 8.0), and salinity (5 to 40‰) all had significant effects on the μ of each biotype. Temperature had the greatest effect on the μ of biotype 1 (CMCP6), whereas salinity had the greatest effect on the μ of biotypes 2 (ATCC 33147) and 3 (302/99). The biotypes' growth rates varied significantly; biotype 1 grew most rapidly, while biotype 3 grew most slowly. The highest growth rates were achieved at 37°C, pH 7.0, and salinities of 15 to 30‰ (μ = 4.0, 2.9, and 2.4 generations h−1for biotypes 1, 2, and 3, respectively). Other strains of the biotypes yielded comparable results, suggesting that the physiological responses of the biotypes are differentially affected by parameters that are highly variable both in estuarine environments and between the free-living and pathogen states ofV. vulnificus.

scholarly journals Biomarkers of a Healthy Nordic Diet—From Dietary Exposure Biomarkers to Microbiota Signatures in the Metabolome

Nutrients ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 27 ◽  
Author(s):  
Rikard Landberg ◽  
Kati Hanhineva
Keyword(s):  
Gut Microbiota ◽  
Observational Studies ◽  
Large Scale ◽  
Dietary Assessment ◽  
Dietary Exposure ◽  
Current Status ◽  
Dietary Interventions ◽  
Nordic Diet ◽  
Health And Disease ◽  
The Impact

Whole diets and dietary patterns are increasingly highlighted in modern nutrition and health research instead of single food items or nutrients alone. The Healthy Nordic Diet is a dietary pattern typically associated with beneficial health outcomes in observational studies, but results from randomized controlled trials are mixed. Dietary assessment is one of the greatest challenges in observational studies and compliance is a major challenge in dietary interventions. During the last decade, research has shown the great importance of the gut microbiota in health and disease. Studies have have both shown that the Nordic diet affects the gut microbiota and that the gut microbiota predicts the effects of such a diet. Rapid technique developments in the area of high-throughput mass spectrometry have enabled the large-scale use of metabolomics both as an objective measurement of dietary intake as well as in providing the final readout of the endogenous metabolic processes and the impact of the gut microbiota. In this review, we give an update on the current status on biomarkers that reflect a Healthy Nordic Diet or individual components thereof (food intake biomarkers), biomarkers that show the effects of a Healthy Nordic Diet and biomarkers reflecting the role of a Healthy Nordic Diet on the gut microbiota as well as how the gut microbiota or derived molecules may be used to predict the effects of a Healthy Nordic Diet on different outcomes.

Herbal dietary interventions for weight loss among regularly exercising women in Turkey

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Elif Didem Ors ◽  
Zeynep Goktas
Keyword(s):  
Weight Loss ◽  
Dietary Intervention ◽  
Fat Free Mass ◽  
Healthy Weight ◽  
Turkish Women ◽  
Dietary Interventions ◽  
Herbal Supplements ◽  
Body Perception ◽  
Content Type ◽  
Exercising Women

Purpose Obesity is a chronic disease that is conjoined with increased mortality. Parallel to rising obesity rates, herbal dietary interventions to lose weight are also increasing. The purpose of this study is to assess and evaluate the use of herbal dietary interventions for weight loss among regularly exercising women. Design/methodology/approach A total of 545 regularly exercising women (aged 19–64 years) were recruited from several local sports centers. A questionnaire form to evaluate demographic characteristics, nutritional habits and herbal dietary intervention habits was administered by a trained dietitian. Anthropometric measurements including weight, height, fat mass, total body water and fat free mass were measured using a bioelectrical impedance analysis device. Findings A total of 244 participants (44.8%) used herbal dietary interventions for weight loss within the last year. The most commonly used herbal dietary interventions were green tea (59.4%), lemon juice (27%), herbal tea mix (20.9%) and cinnamon powder (17.6%), respectively. Of the 244 women who used herbal dietary interventions for weight loss, 42.2% claimed that by using these products they experienced weight loss. Herbal dietary interventions were significantly higher among women with obesity (61.9%) than women with a healthy weight (37.5%). The use of herbal supplements increased among women who consider themselves as being overweight (p < 0.001). Furthermore, herbal dietary interventions decreased with more frequent attempts for a weight-loss diet (p < 0.001). Originality/value To the best of the authors’ knowledge, this study represents a first attempt at assessing the use of herbal dietary supplements for weight loss among regularly exercising Turkish women. With the increase of body mass index and body perception being as overweight, herbal dietary interventions were also increased. In particular, women with obesity (61.9%) used more herbal dietary interventions than women with healthy weight (37.5%). Moreover, women with an overweight body perception used more herbal supplements than those who thought they had a healthy body weight.

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