scholarly journals Two-temperature membrane filter method for enumerating fecal coliform bacteria from chlorinated effluents.

1977 ◽  
Vol 33 (6) ◽  
pp. 1259-1264 ◽  
Author(s):  
B L Green ◽  
E M Clausen ◽  
W Litsky
1975 ◽  
Vol 29 (4) ◽  
pp. 532-536 ◽  
Author(s):  
Robert E. Rose ◽  
Edwin E. Geldreich ◽  
Warren Litsky

1980 ◽  
Vol 43 (7) ◽  
pp. 563-563
Author(s):  
E. P. MERRILL

Of 658 individual water supplies tested over an 8-year period, 69% of drilled wells, 62% of driven wells, 27% of dug wells and 32% of springs were judged acceptable on the basis of a single test for total coliforms. The acceptability standard consisted of 0 to 1 coliforms/100 ml of sample by the membrane filter method or a Most Probable Number index of less than 2.2 (presumptive and confirmed tests).


1975 ◽  
Vol 29 (4) ◽  
pp. 532-536
Author(s):  
Robert E. Rose ◽  
Edwin E. Geldreich ◽  
Warren Litsky

1993 ◽  
Vol 39 (11) ◽  
pp. 1066-1070 ◽  
Author(s):  
Lois C. Shadix ◽  
Michele E. Dunnigan ◽  
Eugene W. Rice

A two-step membrane filter procedure was evaluated to determine the ability to differentiate Escherichia coli from other coliform bacteria recovered from water. M-Endo LES agar incubated at 35 °C for 24 ± 2 h was used as the initial isolation medium. Membranes containing coliform colonies were transferred to nutrient agar plus 4-methylumbelliferyl β-D-glucuronide (MUG) and incubated for an additional 4 h at 35 °C. Escherichia coli colonies were distinguished by fluorescence when viewed under a long-wavelength ultraviolet light. A total of 119 MUG-positive colonies were isolated from 15 water sources, of which 115 (96.6%) were identified as E. coli. An examination of 182 pure culture environmental E. coli isolates revealed that 167 isolates (91.8%) exhibited fluorescence on the nutrient agar plus MUG medium. Survivors of E. coli cultures exposed to chlorination were also capable of producing a positive MUG reaction.Key words: membrane filtration, 4-methylumbelliferyl β-D-glucuronide (MUG), Escherichia coli, water.


2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).


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