scholarly journals Comparison of Colilert-18 with miniaturised most probable number method for monitoring of Escherichia coli in bathing water

2015 ◽  
Vol 14 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Ananda Tiwari ◽  
Seppo I. Niemelä ◽  
Asko Vepsäläinen ◽  
Jarkko Rapala ◽  
Seija Kalso ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
False Negative ◽  
Reference Method ◽  
Water Quality Monitoring ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Negative Results ◽  
Bathing Water

The purpose of this equivalence study was to compare an alternative method, Colilert-18 Quanti-Tray (ISO 9308-2) with the European bathing water directive (2006/7/EC) reference method, the miniaturised most probable number (MMPN) method (ISO 9308-3), for the analysis of Escherichia coli. Six laboratories analysed a total of 263 bathing water samples in Finland. The comparison was carried out according to ISO 17994:2004. The recovery of E. coli using the Colilert-18 method was 7.0% and 8.6% lower than that of the MMPN method after 48 hours and 72 hours of incubation, respectively. The confirmation rate of presumptive E. coli-positive wells in the Colilert-18 and MMPN methods was high (97.8% and 98.0%, respectively). However, the testing of presumptive E. coli-negative but coliform bacteria-positive (yellow but not fluorescent) Colilert-18 wells revealed 7.3% false negative results. There were more false negatives in the naturally contaminated waters than in the samples spiked with waste water. The difference between the recovery of Colilert-18 and the MMPN method was considered not significant, and subsequently the methods are considered as equivalent for bathing water quality monitoring in Finland. Future bathing water method equivalence verification studies may use the data reported herein. The laboratories should make sure that any wells showing even minor fluorescence will be determined as positive for E. coli.

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals Comparison of the Compact Dry EC with the Most Probable Number Method (AOAC Official Method 966.24) for Enumeration of Escherichia coli and Coliform Bacteria in Raw Meats: Performance-Tested MethodSM 110402

2006 ◽  
Vol 89 (1) ◽  
pp. 100-114 ◽  
Author(s):  
Hidemasa Kodaka ◽  
Shingo Mizuochi ◽  
Hajime Teramura ◽  
Tadanobu Nirazuka ◽  
David Goins ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Soluble ◽  
Test Method ◽  
Gelling Agent ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Official Method ◽  
Probable Number ◽  
E Coli ◽  
Raw Meats

Abstract Compact Dry E. coli/Coliform Count (EC) is a ready-to-use test method for the enumeration of Escherichia coli and coliform bacteria in food. The plates are presterilized and contain culture medium and a cold water-soluble gelling agent. The medium should be rehydrated with 1 mL diluted sample inoculated onto the center of the self-diffusible medium, allowing the solution to diffuse by capillary action. The plate can be incubated at 35C for 2024 h and the colonies counted without any further working steps. The Compact Dry EC medium plates were validated as an analysis tool for determining colony-forming units (CFU) of E. coli and coliform bacteria from a variety of raw meats using 5 different types of raw meats. The performance tests were conducted at 35C. In all studies performed, no apparent differences were observed between the Compact Dry ECmethod and theAOAC Official Method 966.24 results. For the accuracy claim (n = 75), a correlation factor of r2 = 0.93 (E. coli) and r2 = 0.93 (coliform bacteria) could be assigned, as stated in the application for Performance-Tested MethodSM.

Comparison of the SimPlate Coliform and Escherichia coli Test with Petrifilm, Three-Tube MPN, and VRBA + MUG Methods for Enumerating Coliforms and E. coli in Food

1998 ◽  
Vol 61 (4) ◽  
pp. 444-449 ◽  
Author(s):  
D. E. TOWNSEND ◽  
R. L. IRVING ◽  
A. NAQUI
Keyword(s):  
Escherichia Coli ◽  
Correlation Coefficients ◽  
Raw Milk ◽  
Food Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Additional Food ◽  
Suitable Alternative ◽  
Probable Number ◽  
E Coli

SimPlate for coliforms and Escherichia coli (CEc) is a new method for the detection and quantification of coliforms and E. coli in food. Internal validation of the method was carried out at IDEXX Laboratories (Westbrook, ME) with 180 food samples representing a variety of different food matrices and compared against three-tube MPN (most probable number), VRBA (violet red bile agar) + MUG, and Petrifilm (E. coli count) methods. SimPlate CEc was highly correlated with each of these methods for the quantification of coliform bacteria (r ≥ 0.90). An insignificant number of food samples were found to contain E. coli; therefore, no meaningful correlation data could be generated. Four hundred forty-four additional food samples were tested at five collaborating laboratories for the presence of coliforms and E. coli using SimPlate CEc and either VRBA + MUG or Petrifilm (E. coli count). Regression analysis of data from SimPlate for CEc versus Petrifilm E. coli count plates generated correlation coefficients (r) of at least 0.89 for total coliforms and at least 0.90 for generic E. coli. Correlation coefficients between SimPlate for CEc and VRBA + MUG data were at least 0.90 for coliforms and at least 0.86 for E. coli. SimPlate for CEc demonstrated better recovery of E. coli than Petrifilm when high populations of bacteria were present. E. coli was not detected in 20 of 50 (40%) raw milk samples tested by the Petrifilm method due to the presence of interfering coliform and noncoliform bacteria. It is concluded that SimPlate for CEc is a suitable alternative for determining numbers of coliform bacteria and E. coli in food.

Incidence and Behavior of Salmonella and Escherichia coli on Whole and Sliced Zucchini Squash (Cucurbita pepo) Fruit

2010 ◽  
Vol 73 (8) ◽  
pp. 1423-1429 ◽  
Author(s):  
JAVIER CASTRO-ROSAS ◽  
EVA MARÍA SANTOS LÓPEZ ◽  
CARLOS ALBERTO GÓMEZ-ALDAPA ◽  
CESAR ABELARDO GONZÁLEZ RAMÍREZ ◽  
JOSÉ ROBERTO VILLAGOMEZ-IBARRA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inoculum Level ◽  
Probable Number ◽  
Initial Inoculum ◽  
E Coli ◽  
Thermotolerant Coliforms ◽  
Salmonella Serotypes ◽  
And Behavior

The incidence of coliform bacteria (CB), thermotolerant coliforms (TC), Escherichia coli, and Salmonella was determined for zucchini squash fruit. In addition, the behavior of four serotypes of Salmonella and a cocktail of three E. coli strains on whole and sliced zucchini squash at 25 ± 2°C and 3 to 5°C was tested. Squash fruit was collected in the markets of Pachuca city, Hidalgo State, Mexico. CB, TC, E. coli, and Salmonella were detected in 100, 70, 62, and 10% of the produce, respectively. The concentration ranged from 3.8 to 7.4 log CFU per sample for CB, and <3 to 1,100 most probable number per sample for TC and E. coli. On whole fruit stored at 25 ± 2°C or 3 to 5°C, no growth was observed for any of the tested microorganisms or cocktails thereof. After 15 days at 25 ± 2°C, the tested Salmonella serotypes had decreased from an initial inoculum level of 7 log CFU to <1 log, and at 3 to 5°C they decreased to approximately 2 log. Survival of E. coli was significantly greater than for the Salmonella strains at the same times and temperatures; after 15 days, at 25 ± 2°C E. coli cocktail strains had decreased to 3.4 log CFU per fruit and at 3 to 5°C they decreased to 3.6 log CFU per fruit. Both the Salmonella serotypes and E. coli strains grew when inoculated onto sliced squash: after 24 h at 25 ± 2°C, both bacteria had grown to approximately 6.5 log CFU per slice. At 3 to 5°C, the bacterial growth was inhibited. The squash may be an important factor contributing to the endemicity of Salmonella in Mexico.

ColiComplete® Substrate-Supporting Disc Method for Confirmed Detection of Total Coliforms and Escherichia coli in All Foods: Comparative Study

1994 ◽  
Vol 77 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Philip T Feldsine ◽  
Maria T Falbo-Nelson ◽  
David L Hustead
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Total Coliforms ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Better Than

Abstract The ColiComplete® substrate-supporting disc (SSD) method for simultaneous confirmed total coliform count and Escherichia coli determination in all foods was compared with the AOAC most probable number (MPN) methods 966.23 and 966.24. In this comparative study, 20 water and food types were analyzed; 7 of these foods were naturally contaminated with coliform bacteria, 6 food types were naturally contaminated with E. coli, and the remaining foods were inoculated with coliform bacteria and/or E. coli. Data were analyzed separately for total coliform bacteria and for E. coli. Mean log MPN counts were determined by the SSD method and the appropriate AOAC MPN procedure. Results were then analyzed for mean log MPN differences and variance, according to methods described by AOAC INTERNATIONAL Results for both total conforms and E. coli indicate that the SSD method is equivalent to or better than AOAC MPN methods 966.23 and 966.24.

scholarly journals Two Rapid Methods for Detection of Escherichia coli Exceeding 104/g Action Levels: Precollaborative Study

2006 ◽  
Vol 89 (5) ◽  
pp. 1317-1326
Author(s):  
Michael A Grant ◽  
Jane S Wernberg ◽  
Khanh T Van ◽  
Angelina M Albert
Keyword(s):  
Escherichia Coli ◽  
Membrane Filtration ◽  
Reference Method ◽  
Most Probable Number ◽  
Lauryl Sulfate ◽  
Probable Number ◽  
E Coli ◽  
Potential Alternative ◽  
Aoac Method ◽  
Action Levels

Abstract The current AOAC Method 966.24 for enumeration of Escherichia coli in foods uses a most probable number (MPN) procedure with extensive confirmation steps. Two new methods based on membrane filtration (MF) were compared to the MPN reference method for detection of high levels of E. coli in 5 food types, some of which represent categories for which the U.S. Food and Drug Administration (FDA) mandates additional testing if an action level of 104/g E. coli is exceeded. Ground beef, which is not FDA regulated, was also tested. The 5 food types were all inoculated at 3 levels: 102/g, ≥104/g, and ≥105/g E. coli. An MF protocol using either m-ColiBlue24® (CB) or lauryl sulfate tryptose plus BCIG (LST/BCIG) was an effective potential alternative to the reference method. Sensitivity and specificity for both CB and LST/BCIG were 98 and 100%, respectively. Agreement between MPN and both CB and LST/BCIG was 98%. The 2 proposed methods allow completion of both presumptive and confirmatory steps in 13 days, whereas the reference method requires as many as 11 days. Exclusivity testing with 50 non-E. coli strains indicated 100% were correctly ruled out by the proposed protocols. Inclusivity testing was used to determine whether typical results were obtained after incubation of E. coli cultures on CB or LST/BCIG for 24 h. Of 50 E. coli strains tested, 100% yielded typical results after incubation on CB, and 98% yielded typical results after incubation on LST/BCIG.

scholarly journals Detection of Escherichia coli Contamination using Most Probable Number (MPN) methods in Chicken Meats in Market of Surabaya

2021 ◽  
Vol 4 (1) ◽  
pp. 118
Author(s):  
Dhandy Koesoemo Wardhana ◽  
Devi Ayu Safitri ◽  
Suwaibatul Annisa ◽  
Mustofa Helmi Effendi ◽  
Nenny Harijani
Keyword(s):  
Escherichia Coli ◽  
Chicken Meat ◽  
Most Probable Number ◽  
Consumer Health ◽  
Meat Processing ◽  
Probable Number ◽  
Minimum Requirement ◽  
E Coli ◽  
Negative Results ◽  
Contaminated Food

Foodborne diseases are diseases transmitted through contaminated food and are one of the main public health problems. The bacteria that usually contaminates chicken meat is Escherichia coli. E. coli contamination in chicken meat can affect consumer health. Chicken meat processing and market conditions affect the level of bacteria contamination. This study aims to measure the contamination of E. coli bacteria in chicken meat in Surabaya's markets. The method which used to count and estimate the number of E. coli bacteria found in chicken meat in this study was Most Probable Number (MPN). The results showed 20 samples (33.3%) from 60 samples gave negative results. This indicated that only 20 samples of chicken meat were safe for consumption because the MPN value is <1 × 101 CFU/g while the remaining 40 samples (66.7%) were not safe for consumption because the MPN value is> 1 × 101 CFU/g. The standard of SNI 3924: 2009 concerning the minimum requirement for the amount of E. coli contamination in chicken meat is 1×101 CFU/g so that the contamination of E. coli which found in chicken meat in Surabaya was high.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Validation of RAPID'E. coli 2 for Enumeration and Differentiation of Escherichia coli and Other Coliform Bacteria in Selected FoodsPerformance-Tested MethodSM 050601

2007 ◽  
Vol 90 (5) ◽  
pp. 1284-1315 ◽  
Author(s):  
Wendy F Lauer ◽  
Frederic L Martinez ◽  
Asmita Patel
Keyword(s):  
Simultaneous Detection ◽  
Reference Method ◽  
Raw Milk ◽  
Coli Strain ◽  
Cottage Cheese ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Fermented Sausage ◽  
Probable Number ◽  
E Coli

Abstract RAPID'E. coli 2 agar (Bio-Rad Laboratories, Hercules, CA) is a chromogenic medium for differentiation and enumeration of E. coli and non-E. coli coliform bacteria in food. The principle of RAPID'E. coli 2 medium relies on simultaneous detection of 2 enzymatic activities, -d-glucuronidase (GLUC) and -d-galactosidase (GAL). Coliforms, other than E. coli (GAL+/GLUC), form blue to green colonies, whereas, specifically, E. coli (GAL+/GLUC+) form violet colonies. Eleven foods (raw ground beef, raw boneless pork, fermented sausage, processed ham, processed turkey, frozen turkey breast, raw ground chicken, cottage cheese, ricotta cheese, raw milk, and dry infant formula) were validated, comparing the performance of RAPID'E. coli 2 agar to the reference method AOAC 966.24. Two sample incubation temperatures were evaluated, 37 and 44C, testing a mixture of naturally and artificially contaminated foods. If naturally contaminated food was not available, thematrix was artificially inoculated with one E. coli strain and one non-E. coli coliform strain. Method comparison studies demonstrated some statistical differences between the 2 methods, which are expected when a plating method is compared to a most probable number method. Inclusivity and exclusivity rates of the medium were 99 and 94, respectively. The RAPID'E. coli 2 method was shown to be stable when minor variations were introduced.

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

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