Formulasi Sabun Cair Dengan Kombinasi Minyak Kelapa Murni (VCO) dan Ekstrak Daun Miana (Coleus atropurpureus)

Saponification between alkaline salt and virgin coconut oil (VCO) will produce liquid soap. This is because the use of virgin coconut oil (VCO) in saponification is known to be very good because of the presence of lauric acid which has clean power and functions as an antimicrobial. The addition of miana leaf extract (Coleus atropurpureus) in the manufacture of liquid soap because it contains flavonoids, tannins, saponins, essential oils which also function as moisturizers and antioxidants. The purpose of this study was to determine the effectiveness of the best combination of pure coconut oil (VCO) liquid soap with variations in the concentration of miana leaf extract (Coleus atropurpureus) in reducing free radicals in the form of bacteria. In this study, a laboratory experimental method was used with the addition of miana leaf extract. A= 0% (w/w), B= 10% (w/w), C= 15% (w/w),) in 100 mL total virgin coconut oil (VCO) three times. The test parameters were in the form of a bacteriological test using the Total Plate Number (ALT) method. From the results of the study, it was found that the formulation of pure coconut oil (VCO) liquid soap with various concentrations of miana leaf extract (Coleus atropurpureus) could reduce free radicals such as bacteria.

Microbiological monitoring as a component of efficient prevention and treatment of purulent-septic infections in an orthopedics and traumatology department

Efficient monitoring of circulating purulent-septic infectious agents in a clinical setting and a study on antibiotic susceptibility of isolated strains of microorganisms allows identifying changes in the pathogen structure and trends in antibiotic resistance development, which helps to determine the tactics of antibacterial therapy and elaborate appropriate measures. The aim of the study. Retrospective analysis of the results of microbiological monitoring of purulent-septic infectious (PSI) agents in the Orthopedics and Traumatology Department (OTD) of the Zaporizhzhia Central Ambulance and Emergency Care Hospital over the period 2017–2020 to determine the main antibacterial drugs for empirical therapy. Materials and methods. We analyzed the bacteriological test results of 664 clinical material samples obtained from OTD patients using bacteriological examination statistical reporting and analytical data of the WHONET 5.6 software. Results. The main PSI pathogens in the OTD were from the ESKAPE group: E. coli, S. aureus, K. pneumoniae, A. baumannii, E. faecalis, P. aeruginosa and S. epidermidis, P. mirabilis, C. amycolatum. Isolates of E. faecalis were sensitive to vancomycin, linezolid, S. aureus – to linezolid, tigecycline, netilmicin, A. baumannii – to tigecycline. All P. aeruginosa strains were resistant to ticarcillin/clavulanate, cefepime, chloramphenicol, imipenem, meropenem, aztreonam, ciprofloxacin. E. coli and K. pneumoniae were resistant to ampicillin, ticarcillin/clavulanate, aztreonam, ceftriaxone, cefepime. The number of isolates sensitive to piperacillin/tazobactam, carbapenems, levofloxacin, gentamicin, amikacin, chloramphenicol ranged from 37 % to 65 %. Conclusions. E. coli, S. aureus, K. pneumoniae, A. baumannii, E. faecalis, P. aeruginosa, S. epidermidis, P. mirabilis, C. amycolatum play an important role in the structure of PSI pathogens in the Orthopedics and Traumatology Department of Zaporizhzhia Central Ambulance and Emergency Care Hospital. The antibiotics of choice as the antibacterial empirical therapy for enterococcal infections are vancomycin, linezolid, for staphylococcal infections – vancomycin, linezolid, tigecycline, netilmicin. PSI pathogens continually evolve developing antibiotic resistance, and it is of particular importance to monitor antibiotic susceptibility of microorganisms within the OTD.

Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods

Background: In cattle, many serotypes of Salmonella enterica are responsible for a wide variety of clinical manifestations, which can cause considerable economic loss. Some serotypes can cause cows to abort sporadically, such as the Dublin serotype. This study was carried out on different cattle farms in the Algiers region to determine the prevalence of Salmonella Dublin using bacteriological and immunological methods.Methodology: The prevalence of Salmonella was determined by bacteriological analysis in accordance with the reference method AFNOR NF U 47-100 on faecal samples collected from 184 cattle belonging to 19 different farms, and serotyping for S. Dublin. Immunological analysis by enzyme-linked immunosorbent assay (ELISA) for S. Dublin was carried out on milk samples collected from 91 cattle. A survey of case (n=5) and control (n=14) farms for comparative analysis was performed to demonstrate a link between abortion in cows and prevalence of S. Dublin with both bacteriological and immunological methods. Sensitivity, specificity, Cohen Kappa coefficient, McNemar test odds ratios, and confidence intervals were calculated using Winepiscope 2.0 and StatA 9.1 software,and p<0.05 was considered as statistically significant.Results: The bacteriological results showed a prevalence of 7.6% (95%CI: 3-10), for Salmonella and serotyping revealed a prevalence for S. Dublin of 2.7%. The immunological analysis of milk by the ELISA technique revealed a prevalence of 13.2% (95%CI: 5-20) for S. Dublin. The comparative study between immunological results from milk and bacteriological results from faeces for detecting S. Dublin showed poor agreement between the two tests (k=0.25), with enzyme immunoassay being significantly more sensitive than the bacteriological test (p<0.05). The results of the survey did not demonstrate a clear association between bacteriological detection of S. Dublin in faeces and abortion in cows (OR=8.66, 95%CI: 0.58-130.12). However, with the immunological analysis of milk for S. Dublin, there was a significant positive association (OR=62.33, 95%CI: 2.13-18.22) between a positive antibody response to S. Dublin in milk and the presence of abortions on the farm.Conclusion: In view of these results, we can conclude that Salmonella infections should systematically feature in the differential diagnosis of abortions in dairy cattle in Algeria. Keywords: S. Dublin, cattle, faeces, milk, abortion, immunology, bacteriology, Algiers

Disease burden of bloodstream infections caused by antimicrobial-resistant bacteria: a population-level study—Japan, 2015-2018

SummaryBackgroundAntimicrobial resistance (AMR) is a global health problem. However, quantitative evaluation of its disease burden is challenging. This study aimed to estimate the disease burden of bloodstream infections (BSIs) caused by major antimicrobial-resistant bacteria in Japan between 2015 and 2018 in terms of disability-adjusted life-years (DALYs).MethodsWe estimated the DALYs of BSIs caused by the six major antimicrobial-resistant bacteria in Japan by utilising comprehensive national surveillance data of all routine bacteriological test results from more than 1,400 hospitals between 2015 and 2018. We modified the methodology of a previous study by Cassini and colleagues to enable comparison of our results with those in other countries.ResultsWe estimated that 135.8 (95% uncertainty interval [UI] 128.6-142.9) DALYs per 100,000 population was attributable to BSIs caused by the six antimicrobial-resistant bacteria in 2018. Staphylococcus aureus (MRSA), fluoroquinolone-resistant Escherichia coli (FQREC), and third-generation cephalosporin-resistant E. coli (3GREC) accounted for 88.6% of the total. The burden did not decrease during the study period and was highest in people aged 65 years or older.ConclusionOur results revealed for the first time the disease burden of BSIs caused by six major antimicrobial-resistant bacteria in Japan. The estimated disease burden associated with AMR in Japan is substantial and has not begun to decrease. Notably, the burden from FQREC and 3GREC has increased steadily and that from MRSA is larger than EU/EEA area, whereas that from other bacteria was comparatively small. Our results are expected to provide useful information for healthcare policymakers for prioritising interventions for AMR.FundingMinistry of Health, Labour and Welfare research grant (20HA2003); Japan Agency for Medical Research and Development Research Program on Emerging and Re-emerging Infectious Diseases (JP19fk0108061)

Cervical Canal Microbiota Features in Second Trimester of Pregnancy in Insuficiencia Istmicocervical with Clinical Signs of Vulvovaginitis Depending on Vaginal Discharge рH

Study Objective: To study the microbiological composition of cervical canal in insuficiencia istmicocervical (IIC) in patients with vulvovaginitis depending on vaginal discharge рH. Study Design: perspective study. Materials and Methods. The study included 67 patients of reproductive age with clinical signs of vulvovaginitis and IIC. Where vaginal discharge рН was > 4.5, patients were prescribed empirical Metrogil Plus; these women comprised the study group (n = 40). Where vaginal discharge рН was ≤ 4.5, patients were prescribed empirical Tergynan; these women were controls (n = 27). Baseline anthropometric, social, clinical and anamnestic data, pregnancy characteristics and parity were recorded; then women underwent examination, and samples were collected for analysis (bacterioscopy, vaginal discharge рН measurement, bacteriological test). On day 9–11 of hospitalisation, patients had another speculum examination; clinical symptoms dynamics, pH and bacterioscopic test results were evaluated. Satisfaction with therapy, compliance and therapy-related arverse events were recorded. Study Results. Various Staphylococcus spp. were the most prevalent cause of the conditions in the study group (n = 17; 42.5%). In controls this value was 12 (44.4%). Staphylococcus spp. were mostly opportunistic saprophytic flora and usually were not pathogens, except for Staphylococcus haemolyticus, which was found in 11 (27.5%) patients in the study group and in 3 (11.1%) controls. Lactobacillus spp. Was diagnosed more often in controls than in the study group: 12 (44.4%) vs. 9 (22.5%) (р = 0.05). The control group was diagnosed only with Lactobacillus crispatus, Lactobacillus jensenii; they also prevailed in the study group, together with Lactobacillus gasseri, Lactobacillus paracasei. At рН > 4.5, cervical canal culture was characterised with a variety of opportunistic pathogens and normal flora. After the therapy, patients in both groups did not complain of vaginal discharges. Physical examination demonstrated normal vaginal wall colour and moderate discharges. Vaginal discharge pH was significantly lower in the study group and was normal in controls. Conclusion. The majority of patients with vulvovaginitis and IIC in the second trimester of pregnancy had lower prevalence as well as lower absolute and relative amount of Lactobacillus spp. in their cervical canal discharge. At the same time, increase in vaginal рН is associated with changes in the quality ratio of lactic acid bacilli. Keywords: cervical canal microbiota, insuficiencia istmicocervical, vaginal discharge рH, vulvovaginitis

Bacteriological quality of commercially prepared fermented Ogi (Akamu) sold in Some Parts of Afikpo Area in Ebonyi State

In this study ogi (akamu) prepared from fermented maize sold in Enohia, Afikpo and Unwana were subjected to bacteriological test together with the laboratory prepared ogi for bacterial quality using the standard microbiological method. The commercial purchased ogi from the markets of (Enohia, Unwana and Afikpo) showed total bacterial growth of 5.6 × 107, 2.0 × 103, 4.2 × 102 and 3.6 × 106 respectively. Staphylococci growth count of 4.2×102, 2.9×102 and 3.5 × 102 were recorded respectively, while Coliform count recorded 2.0×103, 1.2×103 and 1.5×103 and Lactic acid bacterial loads of 3.6×106, 2.7×106 and 3.2 × 106 respectively. The bacteria isolated from the commercial and laboratory fermented pap were Lactobacillus sp, Staphylococcus sp, Leuconostoc sp, Micrococcus sp, Salmonella sp, E. coli, Citrobacter sp and Klebsiella sp. Lactobacillus sp Leuconostoc sp and Citrobacter sp were present in all the samples, Micrococcus sp and Klebsiella sp were isolated from Enohia and Unwana markets ogi respectively, while E. coli and Staphylococcus sp were present in Enohia, Unwana and Afikpo markets. Salmonella sp were isolated from the three markets. No pathogenic bacteria was isolated from the laboratory Prepared ogi (Akamu). Hence, there are chances of contracting food borne diseases from commercially prepared ogi in the local areas of study. Therefore, there is need for sanitary measures in the production of fermented cereals such as ogi so as to minimize the rate of food borne pathogens during processing and storage of such cereals.

Geographical distribution of Enterobacterales with a carbapenemase IMP-6 phenotype and its association with antimicrobial use: An analysis using comprehensive national surveillance data on antimicrobial resistance

Enterobacterales resistant to carbapenems, a class of last-resort antimicrobials, are ranked as an “urgent” and “critical” public health hazard by CDC and WHO. IMP-type carbapenemase-containing Enterobacterales are endemic in Japan, and blaIMP-6 is one of the notable carbapenemase genes responsible for the resistance. The gene is plasmid-encoded and confers resistance to meropenem, but not to imipenem. Therefore, IMP-6-producing Enterobacterales isolates are occasionally overlooked in clinical laboratories and are referred to as ‘stealth-type’. Since previous reports in Japan were confined only to some geographical regions, their distribution across prefectures and the factors affecting the distribution remain unclear. Here, we revealed the dynamics of the geographical distribution of Enterobacterales with IMP-6 phenotype associated with antimicrobial use in Japan. We utilized comprehensive national surveillance data of all routine bacteriological test results from more than 1,400 hospitals in 2015 and 2016 to enumerate Escherichia coli and Klebsiella pneumoniae isolates with the antimicrobial susceptibility pattern (phenotype) characteristic of IMP-6 (imipenem susceptible, meropenem resistant), and to tabulate the frequency of isolates with the phenotype for each prefecture. Isolates were detected in approximately half of all prefectures, and combined analysis with the national data of antimicrobial usage revealed a statistically significant association between the frequency and usage of not carbapenems but third-generation cephalosporins (p = 0.006, logistic mixed-effect regression) and a weaker association between the frequency and usage of fluoroquinolones (p = 0.043). The usage of third-generation cephalosporins and fluoroquinolones may select the strains with the IMP-6 phenotype, and contribute to their occasional spread. We expect the findings will promote antimicrobial stewardship to reduce the spread of the notable carbapenemase gene.

Survey of Salmonella infections in broiler farms in Iran during 2013-2014: a cross-sectional study

Background and Objectives: The aim of this study was to determine the prevalence of Salmonella at broiler breeder farms of Iran and investigate the factors underlying salmonellosis in these farms. This is a cross-sectional investigation conducted in 23 provinces of Iran. Materials and Methods: Fecal samples were collected from 139 broiler breeder farms in the country and standard bacteriological tests were carried out on the samples for the isolation of Salmonella. The serological tests were then applied for the samples that were positive in the bacteriological test. The information on the sampled farms extracted from the Iran GIS-VET Monitoring and Surveillance System was used for the analysis of the risk factors. Results: A total of 11 farms out of the 139 sampled farms were infected with Salmonella with the largest number of infected cases related to Tehran and Fars Provinces. Conclusion: The statistical analysis results showed that flocks with older ages and farms with larger number of houses are at greater risk of Salmonella infection.

Characteristics of migrants to Japan with TB and implications for pre-migration TB screening

Background Japan plans to introduce pre-entry tuberculosis (TB) screening for those traveling from the Philippines, China, Vietnam, Nepal, Indonesia, and Myanmar, who are intending to stay in Japan for more than 90 days, in 2020. According to its screening algorithm, migrants will be screened by chest X-ray and those with abnormal findings will be asked to undergo sputum testing. We analyzed the characteristics of foreign-born TB patients coming from the above six countries, to identify some potential issues. Methods Data on foreign-born patients who had been newly notified with active pulmonary TB (PTB) in 2018 and 2017, and who had entered Japan in the same year they were notified, was extracted from the Japan TB Surveillance system. We assumed that these patients approximated to those hypothetical migrants who would be detected by pre-entry TB screening, and analyzed them by basic socio-demographic characteristics, radiological findings, and bacteriological test results. Results In 2017 and 2018, a total of 1,915 foreign-born PTB patients were notified from the above six countries. Of them, 19.9% (n = 381) had entered Japan in the same year they were notified. Among the 381, 23.9% (n = 50) had cavitary lesion, 27.3% (n = 57) were smear positive, and 48.9% (n = 73) were culture positive. 34.4% (n = 72) were both smear and culture negative, and the diagnosis would have depended on radiographical presentation. However, among the 72, the majority i.e. 87.5%, presented with non-cavitary pulmonary involvement. Conclusions Considering that approximately a third of TB patients who would may detected by the pre-entry screening are likely to be bacteriologically negative, Japan must ensure that the clinics conducting the pre-entry TB screening have access to high-quality radiography with expert interpretations, such as teleradiology and computer-assisted reading. Key messages A third of TB patients who would may detected by the pre-entry screening are likely to be bacteriologically negative. It is critical that clinics conducting screening have access to high-quality radiography with expert interpretations, such as teleradiology and computer-assisted reading.