Design and Evaluation of a Lactobacillus manihotivorans Species-Specific rRNA-Targeted Hybridization Probe and Its Application to the Study of Sour Cassava Fermentation
2000 ◽
Vol 66
(5)
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pp. 2224-2226
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Keyword(s):
ABSTRACT Based on 16S rRNA sequence comparison, we have designed a 20-mer oligonucleotide that targets a region specific to the speciesLactobacillus manihotivorans recently isolated from sour cassava fermentation. The probe recognized the rRNA obtained from all the L. manihotivorans strains tested but did not recognize 56 strains of microorganisms from culture collections or directly isolated from sour cassava, including 29 species of lactic acid bacteria. This probe was then successfully used in quantitative RNA blots and demonstrated the importance of L. manihotivoransin the fermentation of sour cassava starch, which could represent up to 20% of total lactic acid bacteria.
1995 ◽
Vol 21
(2)
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pp. 126-130
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2019 ◽
Vol 56
(9)
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pp. 3969-3979
Keyword(s):
2003 ◽
Vol 66
(3)
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pp. 355-363
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Keyword(s):
2009 ◽
Vol 135
(1)
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pp. 53-59
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Keyword(s):
2005 ◽
Vol 105
(2)
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pp. 213-219
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2014 ◽
Vol 884-885
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pp. 485-489