scholarly journals Fourier Transform Infrared and Raman Spectroscopy for Characterization of Listeria monocytogenes Strains

2006 ◽  
Vol 72 (1) ◽  
pp. 228-232 ◽  
Author(s):  
Astrid Oust ◽  
Trond Møretrø ◽  
Kristine Naterstad ◽  
Ganesh D. Sockalingum ◽  
Isabelle Adt ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Listeria Monocytogenes ◽  
Fragment Length Polymorphism ◽  
Fourier Transform Infrared ◽  
Aflp Analysis ◽  
Aflp Data ◽  
Infrared And Raman Spectroscopy

ABSTRACT The purpose of this study was to characterize the variation in biochemical composition of 89 strains of Listeria monocytogenes with different susceptibilities towards sakacin P, using Fourier transform infrared (FTIR) spectroscopy and Raman spectroscopy. The strains were also analyzed using amplified fragment length polymorphism (AFLP) analysis. Based on their susceptibilities to sakacin P, the 89 strains have previously been divided into two groups. Using the FTIR spectra and AFLP data, the strains were basically differentiated into the same two groups. Analyses of the FTIR and Raman spectra revealed that the strains in the two groups contained differences in the compositions of carbohydrates and fatty acids. The relevance of the variation in the composition of carbohydrates with respect to the variation in the susceptibility towards sakacin P for the L. monocytogenes strains is discussed.

Characterization of the Adsorption of Nucleic Acid Bases onto Ferrihydrite via Fourier Transform Infrared and Surface-Enhanced Raman Spectroscopy and X-ray Diffractometry

Astrobiology ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 728-738 ◽  
Author(s):  
José E. Canhisares-Filho ◽  
Cristine E.A. Carneiro ◽  
Henrique de Santana ◽  
Alexandre Urbano ◽  
Antonio C.S. da Costa ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Nucleic Acid ◽  
Fourier Transform Infrared ◽  
Surface Enhanced Raman Spectroscopy ◽  
Nucleic Acid Bases ◽  
X Ray ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Determination of structural changes in microwaved rice starch using Fourier transform infrared and Raman spectroscopy

2012 ◽  
Vol 64 (8) ◽  
pp. 598-606 ◽  
Author(s):  
Daming Fan ◽  
Wenrui Ma ◽  
Liyun Wang ◽  
Jianlian Huang ◽  
Jianxin Zhao ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Structural Changes ◽  
Fourier Transform Infrared ◽  
Rice Starch ◽  
Infrared And Raman Spectroscopy

scholarly journals Characterization of Verticillium albo-atrum Field Isolates Using Pathogenicity Data and AFLP Analysis

Plant Disease ◽  
2003 ◽  
Vol 87 (6) ◽  
pp. 633-638 ◽  
Author(s):  
Sebastjan Radišek ◽  
Jernej Jakše ◽  
Andrej Simončič ◽  
Branka Javornik
Keyword(s):  
Fragment Length Polymorphism ◽  
Economic Losses ◽  
Aflp Analysis ◽  
Mild Form ◽  
Aflp Data ◽  
Green Pepper ◽  
Clear Separation ◽  
Pathogenicity Tests ◽  
Verticillium Albo Atrum

Since 1997, hop wilt induced by a virulent pathotype of Verticillium albo-atrum has caused considerable economic losses in hop fields in Slovenia. In all, 20 isolates of V. albo-atrum, including 12 from plants affected with the lethal form (PG2) of hop wilt, 6 from plants with the mild form (PG1), 1 from cucumber, and 1 from petunia, as well as 1 isolate of V. dahliae each from hop and green pepper, were analyzed by amplified fragment length polymorphism (AFLP). Differences in the virulence of hop isolates were confirmed by pathogenicity tests on hop cultivars. The AFLP method was optimized for analysis of these fungi and 7 of 39 primer combinations tested were used for the analysis of polymorphism among isolates. Cluster analysis of AFLP data divided the isolates into two, well-separated V. albo-atrum and V. dahliae clusters, confirming that the two species are genetically distinct. Within the V. albo-atrum cluster, isolates were further separated into two distinct groups: the A1 group contained PG1 hop pathotype and cucumber and petunia isolates, and the A2 group all hop isolates of the PG2 pathotype. Minor genetic variation was detected within pathotype-associated AFLP groups, but the clear separation of V. albo-atrum hop isolates according to their level of virulence shows genetic differentiation among hop V. albo-atrum pathotypes.

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