Specific Antibodies in Sera and Gastric Aspirates of Symptomatic and Asymptomatic Helicobacter pylori-Infected Subjects

ABSTRACT In this study we have determined systemic and local antibody responses against different Helicobacter pylori antigens inH. pylori-infected and noninfected subjects. In addition, we studied whether differences in antibody responses between patients with duodenal ulcers and asymptomatic H. pylori carriers might explain the different outcomes of infection. Sera and in most instances gastric aspirates were collected from 19 duodenal ulcer patients, 15 asymptomatic H. pylori carriers, and 20 noninfected subjects and assayed for specific antibodies against different H. pylori antigens, i.e., whole membrane proteins (MP), lipopolysaccharides, flagellin, urease, the neuraminyllactose binding hemagglutinin HpaA, and a 26-kDa protein, by enzyme-linked immunosorbent assay. The H. pylori-infected subjects had significantly higher antibody titers against MP, flagellin, and urease in both sera and gastric aspirates compared with the noninfected subjects. Furthermore, the antibody titers against HpaA were significantly elevated in sera but not in gastric aspirates from the infected subjects. However, no differences in antibody titers against any of the tested antigens could be detected between the duodenal ulcer patients and the asymptomatic H. pyloricarriers, either in sera or in gastric aspirates.

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Prevalence of Helicobacter pylori infection among patients undergoing upper gastrointestinal endoscopy

Asian Journal of Health Sciences ◽

10.15419/ajhs.v3i1.405 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Vudumula Vijaya Lakshmi

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Normal Population ◽

Gastric Ulcers ◽

Upper Gastrointestinal Endoscopy ◽

Contributing Factors ◽

Duodenal Ulcers ◽

Female Ratio ◽

Ulcer Disease ◽

H Pylori

Helicobacter pylori (H. pylori) has a role in the multifactorial etiology of peptic ulcer disease. A link between H. pylori infection and duodenal ulcer disease is now established. Other contributing factors and their interaction with the organism may initiate the ulcerative process. The fact that eradication of H. pylori infection leads to a long-term cure in the majority of duodenal ulcer patients and the fact that the prevalence of infection is higher in ulcer patients than in the normal population are cogent arguments in favor of it being the primary cause of the ulceration. This study was under taken at the Department of surgery, Narayana medical college, Nellore from January 2007 to July 2008. A total of 150 patients with duodenal ulcers, gastric ulcers, antral gastritis, gastric carcinoma and dyspepsia of any kind were studied. Maximum number of cases were in the age group of 31 years to 50 years among both sexes and number of cases gradually decreased after 50 years of age in males and females. Males were more in number and male to female ratio is (2.75:1) approximately 3:1.

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ABO blood group, secretor status and detection ofHelicobacter pyloriamong patients with gastric or duodenal ulcers

Epidemiology and Infection ◽

10.1017/s0950268800048366 ◽

1991 ◽

Vol 106 (2) ◽

pp. 221-229 ◽

Cited By ~ 50

Author(s):

A. Mentis ◽

C. C. Blackwell ◽

D. M. Weir ◽

C. Spiliadis ◽

A. Dailianas ◽

...

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Blood Group ◽

Local Population ◽

Abo Blood Group ◽

Duodenal Ulcers ◽

Secretor Status ◽

Gastric Or Duodenal Ulcers ◽

H Pylori

SUMMARYPatients (454) referred for gastroscopy to the General Hospital of Athens were examined to determine (1) if non-secretors were over-represented among patients with ulcers and (2) is there was an association with ABO blood group or secretor status and carriage ofHelicobacter pylori.Compared with the local population, among patients with either gastric ulcer (51) or duodenal ulcer (96) there was a significant increase in the proportion of those who were blood group O (P< 0·025); however, there were no significant differences in the proportions of non-secretors.H. pyloriwas identified in 62 % of the 454 patients: 59·5 % of those without evidence of ulcers; 62·5 % of those with gastric ulcer; 88% of those with duodenal ulcer (P< 0·0005). These bacteria were cultured more often and in higher numbers from patients with duodenal ulcer (P< 0·025). There was no association between ABO blood group and prevalence ofH. pylori. The prevalence ofH. pyloriamong non-secretors with gastric ulcer (12·5%) was significantly lower than that for non-secretors with duodenal ulcer (100%) (P< 0·0005). This was not observed for secretors.

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A Modified Enzyme-Linked Immunosorbent Assay for Measurement of Antibody Responses to Meningococcal C Polysaccharide That Correlate with Bactericidal Responses

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.5.4.479-485.1998 ◽

1998 ◽

Vol 5 (4) ◽

pp. 479-485 ◽

Cited By ~ 78

Author(s):

Dan M. Granoff ◽

Susan E. Maslanka ◽

George M. Carlone ◽

Brian D. Plikaytis ◽

George F. Santos ◽

...

Keyword(s):

Immunosorbent Assay ◽

Geometric Mean ◽

Correlation Coefficients ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

High Avidity ◽

Igg Antibody ◽

Standard Assay ◽

Bactericidal Antibody ◽

Antibody Titers

ABSTRACT The standardized enzyme-linked immunosorbent assay (ELISA) for measurement of serum immunoglobulin G (IgG) antibody responses to meningococcal C polysaccharide has been modified to employ assay conditions that ensure specificity and favor detection primarily of high-avidity antibodies. The modified and standard assays were used to measure IgG antibody concentrations in sera of toddlers vaccinated with meningococcal polysaccharide vaccine or a meningococcal C conjugate vaccine. The results were compared to the respective complement-mediated bactericidal antibody titers. In sera obtained after one or two doses of vaccine, the correlation coefficients, r, for the results of the standard assay and bactericidal antibody titers were 0.45 and 0.29, compared to 0.85 and 0.87, respectively, for the modified assay. With the standard assay, there were no significant differences between the geometric mean antibody responses of the two vaccine groups. In contrast, with the modified assay, 5- to 20-fold higher postvaccination antibody concentrations were measured in the conjugate than in the polysaccharide group. Importantly, the results of the modified assay, but not the standard ELISA, paralleled the respective geometric mean bactericidal antibody titers. Thus, by employing conditions that favor detection of higher-avidity IgG antibody, the modified ELISA provides results that correlate closely with measurements of antibody functional activity that are thought to be important in protection against meningococcal disease.

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Heterogeneous Antibody Responses in Tuberculosis

Infection and Immunity ◽

10.1128/iai.66.8.3936-3940.1998 ◽

1998 ◽

Vol 66 (8) ◽

pp. 3936-3940 ◽

Cited By ~ 162

Author(s):

Konstantin Lyashchenko ◽

Roberto Colangeli ◽

Michel Houde ◽

Hamdan Al Jahdali ◽

Dick Menzies ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Antibody Response ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Antigen Recognition ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Protein Antigens ◽

Tuberculosis Patients ◽

Specific Antibodies

ABSTRACT Antibody responses during tuberculosis were analyzed by an enzyme-linked immunosorbent assay with a panel of 10 protein antigens of Mycobacterium tuberculosis. It was shown that serum immunoglobulin G antibodies were produced against a variety of M. tuberculosis antigens and that the vast majority of sera from tuberculosis patients contained antibodies against one or more M. tuberculosis antigens. The number and the species of serologically reactive antigens varied greatly from individual to individual. In a given serum, the level of specific antibodies also varied with the antigen irrespective of the total number of antigens recognized by that particular serum. These findings indicate that person-to-person heterogeneity of antigen recognition, rather than recognition of particular antigens, is a key attribute of the antibody response in tuberculosis.

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Immunoblot Analysis of Humoral Immune Response to Helicobacter pylori in Children with and without Duodenal Ulcer

Journal of Clinical Microbiology ◽

10.1128/jcm.38.5.1777-1781.2000 ◽

2000 ◽

Vol 38 (5) ◽

pp. 1777-1781 ◽

Cited By ~ 24

Author(s):

Gifone A. Rocha ◽

Andreia M. R. Oliveira ◽

Dulciene M. M. Queiroz ◽

Anfrisina S. T. Carvalho ◽

Ana M. M. F. Nogueira

Keyword(s):

Immune Response ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Humoral Immune Response ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Method ◽

Predictive Values ◽

Humoral Immune ◽

Urease Test ◽

H Pylori

Several studies have demonstrated that enzyme-linked immunosorbent assay is not a sensitive and specific method to diagnoseHelicobacter pylori infection in children, especially in the younger ones. Since serum immune response can also be determined by immunoblotting and it permits the detection of antibodies to virulence factors such as CagA and VacA, we evaluated the accuracy of a commercial immunoblotting test to diagnose H. pyloriinfection and to assess the humoral immune response to differentH. pylori antigens in 122 children who underwent upper gastrointestinal endoscopy. The presence of H. pylori was determined in antral biopsy specimens by culture, preformed urease test, and histological analysis. H. pylori was identified by microbiological and histopathological methods in 66 children (including all of the 21 who had duodenal ulcer). Antibodies toH. pylori were detected in 63 infected children and in 8 noninfected ones. The sensitivity, specificity, and positive and negative predictive values of the immunoblotting test were 95.5, 85.7, 88.7, and 94.1%, respectively. The number of immunoreactive bands increased with age (P = 0.003), and the bands of 35 kDa (P = 0.013); 89 kDa, the VacA antigen (P = 0.001); and 116 kDa, the CagA antigen (P = 0.00004) were more frequently observed in older children. The frequency of the bands of 89 kDa (P = 0.001) and 116 kDa (P = 0.03) was higher in children with duodenal ulcer than in H. pylori-positive children without the disease. In conclusion, the immunoblotting test appears to be useful for the diagnosis of H. pylori infection in children, even in the younger ones.

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Limitations of Pneumovax® as a detection antigen in the measurement of serotype-specific antibodies by enzyme-linked immunosorbent assay

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1258/000456302760042164 ◽

2002 ◽

Vol 39 (4) ◽

pp. 398-403 ◽

Cited By ~ 4

Author(s):

ZM Huo ◽

J Miles ◽

PG Riches ◽

T Harris

Keyword(s):

Immunosorbent Assay ◽

Specific Antibody ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Carbohydrate Antigens ◽

Specific Antibodies ◽

Elisa System ◽

Background Measurement ◽

Polysaccharide Antigens ◽

The Individual

Background: Measurement of antibody responses to polysaccharide antigens is regarded as an important assessment of an individual's ability to respond to carbohydrate antigens. The currently used assays for the measurement of pneumococcal-specific antibody use the multi-serotype vaccine Pneumovax® as the detection antigen. Methods: An equal potency enzyme-linked immunosorbent assay (ELISA) system was used to compare the measurement of serotype-specific antibody with the multi-serotype assay. Results: Our results show that the concentration of specific antibody to Pneumovax is not related to the concentration of antibody to the individual serotypes. Neither is any correlation found between the antibody concentrations to any of the three single serotypes investigated, to the mixture of the three serotypes or to Pneumovax. Conclusion: We conclude that the measurement of the concentration of the specific antibody to the mixed serotypes present in Pneumovax has serious limitations when used to evaluate the protection acquired from Pneumovax immunization against any specific serotype.

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Immunoglobulin G Antibody Response to Infection with Coccoid Forms of Helicobacter pylori

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.5.1067-1071.2002 ◽

2002 ◽

Vol 9 (5) ◽

pp. 1067-1071 ◽

Cited By ~ 4

Author(s):

G. Figueroa ◽

G. Faúndez ◽

M. Troncoso ◽

P. Navarrete ◽

M. S. Toledo

Keyword(s):

Helicobacter Pylori ◽

Immunoglobulin G ◽

Gel Electrophoresis ◽

Potential Role ◽

Enzyme Linked Immunosorbent Assay ◽

Nonulcer Dyspepsia ◽

Duodenal Ulcers ◽

One Dimensional ◽

H Pylori ◽

Asymptomatic Individuals

ABSTRACT An increasing number of studies support a potential role for coccoid forms in Helicobacter pylori infection. Evidence for this was obtained through scanning microscopy, genetic analysis for virulence traits, examination of the presence and activity of key enzymes, and other methods. We studied the serum immunoglobulin G responses to coccoid H. pylori forms by enzyme-linked immunosorbent assay (ELISA) and immunoblotting and compared them with those of bacillary cells. Sera from a total of 295 infected individuals were studied; these included sera from 100 patients with duodenal ulcers, 98 patients with nonulcer dyspepsia, 11 patients with gastroduodenal cancer, and 86 asymptomatic individuals. Initially, we characterized and selected coccoid and bacillary antigenic preparations by one-dimensional (1-D) and 2-D gel electrophoresis and immunoblotting. Data showed that coccoid and bacillary preparations with comparable protein contents have similar patterns in 1-D and 2-D electrophoresis gels and antigenic recognition at blotting. These results revealed that coccoid and spiral antigens in ELISA can equally recognize specific antibodies to H. pylori in sera from infected individuals. The analysis of the spiral and coccoid preparations by Western blotting showed no major differences in antigen recognition. No specific bands or profiles associated with a single gastric condition were identified.

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Evaluation of Enzyme-Linked Immunosorbent Assay for the Diagnosis of Helicobacter pylori Infection in Children From Different Age Groups With and Without Duodenal Ulcer

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/00005176-199902000-00012 ◽

1999 ◽

Vol 28 (2) ◽

pp. 157-161 ◽

Cited By ~ 83

Author(s):

Andreia Maria Rocha de Oliveira ◽

Gifone Aguiar Rocha ◽

Dulciene Maria de Magalhães Queiroz ◽

Edilberto Nogueira Mendes ◽

Anfrisina Sales Teles de Carvalho ◽

...

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Immunosorbent Assay ◽

Age Groups ◽

Helicobacter Pylori Infection ◽

Enzyme Linked Immunosorbent Assay

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Latex Agglutination Turbidimetric Immunoassay Versus Enzyme-Linked Immunosorbent Assay for Helicobacter pylori: An Observational Study.

10.21203/rs.3.rs-120423/v1 ◽

2020 ◽

Author(s):

Yoshitaka Tokai ◽

Junko Fujisaki ◽

Naoki Ishizuka ◽

Hiroki Osumi ◽

Ken Namikawa ◽

...

Keyword(s):

Helicobacter Pylori ◽

Diagnostic Accuracy ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Latex Agglutination ◽

Concordance Rate ◽

Enzyme Linked Immunosorbent Assay ◽

H Pylori ◽

Antibody Levels ◽

Turbidimetric Immunoassay

Abstract Background: Helicobacter pylori antibody levels in the blood are currently measured using an enzyme-linked immunosorbent assay (ELISA). In April 2016, FUJIFILM Wako Pure Chemical Corporation launched the “L-type Wako Helicobacter pylori antibody J” test, which is based on the latex agglutination turbidimetric immunoassay. In this study, we investigated the usefulness of the Wako test. Methods: We measured H. pylori antibody levels using both ELISA and Wako tests in 180 patients who underwent esophagogastroduodenoscopy at our hospital between September 2017 and February 2019. Ninety patients had H. pylori infections. We calculated the diagnostic accuracy, sensitivity, and specificity of each test and the concordance rate between the two tests. If the lower limits of 90% confidence intervals (CI) for each diagnostic validity exceeded the 85% threshold, the usefulness of the diagnostic test was confirmed. Results: Diagnostic accuracy, sensitivity, and specificity were 94.4% (90% CI; 90.8–97.0%), 94.4% (90% CI; 88.7–97.8%), and 94.4% (90% CI; 88.7–97.8%), respectively, using the Wako test, and 94.4% (90% CI; 90.8–97.0%), 88.9% (90% CI; 81.9–93.8%), and 100% (90% CI; 96.0–100%), respectively, using ELISA. The concordance rate between the two tests was high (κ = 0.8444). Conclusions: We confirmed the usefulness of the Wako test, especially when screening for H. pylori infection, due to its high sensitivity.Trial registration: We retrospectively registered the data of this study.

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Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Pharmacia ◽

10.3897/pharmacia.67.e52568 ◽

2020 ◽

Vol 67 (4) ◽

pp. 393-396

Author(s):

Mykola V. Rybalkin ◽

Natalya V. Khokhlenkova ◽

Julia M. Azarenko ◽

Tatiana V. Diadiun ◽

Svitlana M. Kovalenko

Keyword(s):

Candida Albicans ◽

Therapeutic Effect ◽

Immunosorbent Assay ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Immune Protection ◽

Specific Antibodies ◽

Antibody Titers ◽

Stimulation Of

Therapeutic effect of C. albicans proteins in concentrations 1, 2, 3, 4 and 5 mg/ml has been examined in white mice. Animals were infected i.p. with suspension of C. albicans strain CCM 335–867 in the amount of 20 million cells per 1 ml volume. After 5 days and repeatedly after 14 days mice were injected s.c. in the upper part of right hind paw with proteins of Candida cells of volume 0.2 ml. 14 days after each injection, the determination of the animal body protective functions has been carried out by the titer of specific antibodies during immunoassay. According to the data obtained, it has been found that in the s.c. administration, after the first and second injection with C. albicans protein of concentration 1, 2, 3, 4 and 5 mg/ml antibody titers increased 2 times, indicating that there is no stimulation of immune protection.

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